P08465 (MET2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine O-acetyltransferase
EC=2.3.1.31

Alternative name(s):
Homoserine O-trans-acetylase

Gene names

Name:	MET2
Ordered Locus Names:	YNL277W
ORF Names:	N0615

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.
Miscellaneous	Present with 2240 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the AB hydrolase superfamily. HTA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	homoserine metabolic process Inferred from direct assay PubMed 3301801. Source: SGD methionine biosynthetic process Inferred from mutant phenotype PubMed 6035500. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11914276. Source: SGD
Molecular_function	homoserine O-acetyltransferase activity Inferred from mutant phenotype PubMed 3301801. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Homoserine O-acetyltransferase		PRO_0000155758
Sites
	Active site	162	1	Potential
	Active site	430	1	Potential
Experimental info
	Sequence conflict	429 – 486	58	GHDAF…EVTNW → ATMPSYWSLS Ref.1
	Sequence conflict	429 – 486	58	GHDAF…EVTNW → ATMPSYWSLS Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08465 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: FD5E8B9D0CE8C707	FASTA	486	53,659
	10 20 30 40 50 60 MSHTLKSKTL QELDIEEIKE TNPLLKLVQG QRIVQVPELV LESGVVINNF PIAYKTWGTL 70 80 90 100 110 120 NEAGDNVLVI CHALTGSADV ADWWGPLLGN DLAFDPSRFF IICLNSMGSP YGSFSPLTIN 130 140 150 160 170 180 EETGVRYGPE FPLCTVRDDV RAHRIVLDSL GVKSIACVIG GSMGGMLSLE WAAMYGKEYV 190 200 210 220 230 240 KNMVALATSA RHSAWCISWS EAQRQSIYSD PNYLDGYYPV EEQPVAGLSA ARMSALLTYR 250 260 270 280 290 300 TRNSFENKFS RRSPSIAQQQ KAQREETRKP STVSEHSLQI HNDGYKTKAS TAIAGISGQK 310 320 330 340 350 360 GQSVVSTASS SDSLNSSTSM TSVSSVTGEV KDIKPAQTYF SAQSYLRYQG TKFINRFDAN 370 380 390 400 410 420 CYIAITRKLD THDLARDRVD DITEVLSTIQ QPSLIIGIQS DGLFTYSEQE FLAEHIPKSQ 430 440 450 460 470 480 LEKIESPEGH DAFLLEFKLI NKLIVQFLKT NCKAITDAAP RAWGGDVGND ETKTSVFGEA EEVTNW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence." Langin T., Faugeron G., Goyon C., Nicolas A., Rossignol J.-L. Gene 49:283-293(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Hell R. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15675 Genomic DNA. Translation: AAA34775.1. AJ001940 Genomic DNA. Translation: CAA05109.1. Z71553 Genomic DNA. Translation: CAA96188.1. BK006947 Genomic DNA. Translation: DAA10284.1.
PIR	S63251.
RefSeq	NP_014122.1. NM_001183115.1.
3D structure databases
ProteinModelPortal	P08465.
SMR	P08465. Positions 28-249, 341-448.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3985N.
IntAct	P08465. 2 interactions.
MINT	MINT-525497.
STRING	4932.YNL277W.
Protein family/group databases
MEROPS	S33.A41.
Proteomic databases
PaxDb	P08465.
Protocols and materials databases
DNASU	855444.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL277W; YNL277W; YNL277W.
GeneID	855444.
KEGG	sce:YNL277W.
Organism-specific databases
CYGD	YNL277w.
SGD	S000005221. MET2.
Phylogenomic databases
eggNOG	COG2021.
GeneTree	ENSGT00530000067193.
HOGENOM	HOG000246301.
KO	K00641.
OMA	YGPEFPL.
OrthoDB	EOG4FBN2F.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-441.
UniPathway	UPA00051; UER00074.
Gene expression databases
Genevestigator	P08465.
GermOnline	YNL277W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR008220. Homoserine_AcTrfase. [Graphical view]
Pfam	PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PIRSF	PIRSF000443. Homoser_Ac_trans. 1 hit.
TIGRFAMs	TIGR01392. homoserO_Ac_trn. 1 hit.
ProtoNet	Search...
Other
NextBio	979343.

Entry information

Entry name

MET2_YEAST

Accession

Primary (citable) accession number: P08465
Secondary accession number(s): D6W0R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program