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P08465

- MET2_YEAST

UniProt

P08465 - MET2_YEAST

Protein

Homoserine O-acetyltransferase

Gene

MET2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei162 – 1621Sequence Analysis
    Active sitei430 – 4301Sequence Analysis

    GO - Molecular functioni

    1. homoserine O-acetyltransferase activity Source: SGD

    GO - Biological processi

    1. homoserine metabolic process Source: SGD
    2. methionine biosynthetic process Source: SGD
    3. sulfur amino acid metabolic process Source: SGD

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-441.
    YEAST:YNL277W-MONOMER.
    UniPathwayiUPA00051; UER00074.

    Protein family/group databases

    MEROPSiS33.A41.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homoserine O-acetyltransferase (EC:2.3.1.31)
    Alternative name(s):
    Homoserine O-trans-acetylase
    Gene namesi
    Name:MET2
    Ordered Locus Names:YNL277W
    ORF Names:N0615
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL277w.
    SGDiS000005221. MET2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 486486Homoserine O-acetyltransferasePRO_0000155758Add
    BLAST

    Proteomic databases

    MaxQBiP08465.
    PaxDbiP08465.

    Expressioni

    Gene expression databases

    GenevestigatoriP08465.

    Interactioni

    Protein-protein interaction databases

    BioGridi35564. 11 interactions.
    DIPiDIP-3985N.
    IntActiP08465. 2 interactions.
    MINTiMINT-525497.
    STRINGi4932.YNL277W.

    Structurei

    3D structure databases

    ProteinModelPortaliP08465.
    SMRiP08465. Positions 28-249, 341-448.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. HTA family.Curated

    Phylogenomic databases

    eggNOGiCOG2021.
    GeneTreeiENSGT00530000067193.
    HOGENOMiHOG000246301.
    KOiK00641.
    OMAiNRFDANC.
    OrthoDBiEOG7M3J94.

    Family and domain databases

    Gene3Di3.40.50.1820. 2 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR008220. Homoserine_AcTrfase.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000443. Homoser_Ac_trans. 1 hit.
    SUPFAMiSSF53474. SSF53474. 2 hits.
    TIGRFAMsiTIGR01392. homoserO_Ac_trn. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P08465-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHTLKSKTL QELDIEEIKE TNPLLKLVQG QRIVQVPELV LESGVVINNF    50
    PIAYKTWGTL NEAGDNVLVI CHALTGSADV ADWWGPLLGN DLAFDPSRFF 100
    IICLNSMGSP YGSFSPLTIN EETGVRYGPE FPLCTVRDDV RAHRIVLDSL 150
    GVKSIACVIG GSMGGMLSLE WAAMYGKEYV KNMVALATSA RHSAWCISWS 200
    EAQRQSIYSD PNYLDGYYPV EEQPVAGLSA ARMSALLTYR TRNSFENKFS 250
    RRSPSIAQQQ KAQREETRKP STVSEHSLQI HNDGYKTKAS TAIAGISGQK 300
    GQSVVSTASS SDSLNSSTSM TSVSSVTGEV KDIKPAQTYF SAQSYLRYQG 350
    TKFINRFDAN CYIAITRKLD THDLARDRVD DITEVLSTIQ QPSLIIGIQS 400
    DGLFTYSEQE FLAEHIPKSQ LEKIESPEGH DAFLLEFKLI NKLIVQFLKT 450
    NCKAITDAAP RAWGGDVGND ETKTSVFGEA EEVTNW 486
    Length:486
    Mass (Da):53,659
    Last modified:October 1, 1996 - v2
    Checksum:iFD5E8B9D0CE8C707
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti429 – 48658GHDAF…EVTNW → ATMPSYWSLS(PubMed:3552887)CuratedAdd
    BLAST
    Sequence conflicti429 – 48658GHDAF…EVTNW → ATMPSYWSLS1 PublicationCuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15675 Genomic DNA. Translation: AAA34775.1.
    AJ001940 Genomic DNA. Translation: CAA05109.1.
    Z71553 Genomic DNA. Translation: CAA96188.1.
    BK006947 Genomic DNA. Translation: DAA10284.1.
    PIRiS63251.
    RefSeqiNP_014122.1. NM_001183115.1.

    Genome annotation databases

    EnsemblFungiiYNL277W; YNL277W; YNL277W.
    GeneIDi855444.
    KEGGisce:YNL277W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15675 Genomic DNA. Translation: AAA34775.1 .
    AJ001940 Genomic DNA. Translation: CAA05109.1 .
    Z71553 Genomic DNA. Translation: CAA96188.1 .
    BK006947 Genomic DNA. Translation: DAA10284.1 .
    PIRi S63251.
    RefSeqi NP_014122.1. NM_001183115.1.

    3D structure databases

    ProteinModelPortali P08465.
    SMRi P08465. Positions 28-249, 341-448.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35564. 11 interactions.
    DIPi DIP-3985N.
    IntActi P08465. 2 interactions.
    MINTi MINT-525497.
    STRINGi 4932.YNL277W.

    Protein family/group databases

    MEROPSi S33.A41.

    Proteomic databases

    MaxQBi P08465.
    PaxDbi P08465.

    Protocols and materials databases

    DNASUi 855444.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL277W ; YNL277W ; YNL277W .
    GeneIDi 855444.
    KEGGi sce:YNL277W.

    Organism-specific databases

    CYGDi YNL277w.
    SGDi S000005221. MET2.

    Phylogenomic databases

    eggNOGi COG2021.
    GeneTreei ENSGT00530000067193.
    HOGENOMi HOG000246301.
    KOi K00641.
    OMAi NRFDANC.
    OrthoDBi EOG7M3J94.

    Enzyme and pathway databases

    UniPathwayi UPA00051 ; UER00074 .
    BioCyci MetaCyc:MONOMER-441.
    YEAST:YNL277W-MONOMER.

    Miscellaneous databases

    NextBioi 979343.

    Gene expression databases

    Genevestigatori P08465.

    Family and domain databases

    Gene3Di 3.40.50.1820. 2 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR008220. Homoserine_AcTrfase.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000443. Homoser_Ac_trans. 1 hit.
    SUPFAMi SSF53474. SSF53474. 2 hits.
    TIGRFAMsi TIGR01392. homoserO_Ac_trn. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence."
      Langin T., Faugeron G., Goyon C., Nicolas A., Rossignol J.-L.
      Gene 49:283-293(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Hell R.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMET2_YEAST
    AccessioniPrimary (citable) accession number: P08465
    Secondary accession number(s): D6W0R8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2240 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3