Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P08465 (MET2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homoserine O-acetyltransferase

EC=2.3.1.31
Alternative name(s):
Homoserine O-trans-acetylase
Gene names
Name:MET2
Ordered Locus Names:YNL277W
ORF Names:N0615
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.

Miscellaneous

Present with 2240 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the AB hydrolase superfamily. HTA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Homoserine O-acetyltransferase
PRO_0000155758

Sites

Active site1621 Potential
Active site4301 Potential

Experimental info

Sequence conflict429 – 48658GHDAF…EVTNW → ATMPSYWSLS Ref.1
Sequence conflict429 – 48658GHDAF…EVTNW → ATMPSYWSLS Ref.2

Sequences

Sequence LengthMass (Da)Tools
P08465 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: FD5E8B9D0CE8C707

FASTA48653,659
        10         20         30         40         50         60 
MSHTLKSKTL QELDIEEIKE TNPLLKLVQG QRIVQVPELV LESGVVINNF PIAYKTWGTL 

        70         80         90        100        110        120 
NEAGDNVLVI CHALTGSADV ADWWGPLLGN DLAFDPSRFF IICLNSMGSP YGSFSPLTIN 

       130        140        150        160        170        180 
EETGVRYGPE FPLCTVRDDV RAHRIVLDSL GVKSIACVIG GSMGGMLSLE WAAMYGKEYV 

       190        200        210        220        230        240 
KNMVALATSA RHSAWCISWS EAQRQSIYSD PNYLDGYYPV EEQPVAGLSA ARMSALLTYR 

       250        260        270        280        290        300 
TRNSFENKFS RRSPSIAQQQ KAQREETRKP STVSEHSLQI HNDGYKTKAS TAIAGISGQK 

       310        320        330        340        350        360 
GQSVVSTASS SDSLNSSTSM TSVSSVTGEV KDIKPAQTYF SAQSYLRYQG TKFINRFDAN 

       370        380        390        400        410        420 
CYIAITRKLD THDLARDRVD DITEVLSTIQ QPSLIIGIQS DGLFTYSEQE FLAEHIPKSQ 

       430        440        450        460        470        480 
LEKIESPEGH DAFLLEFKLI NKLIVQFLKT NCKAITDAAP RAWGGDVGND ETKTSVFGEA 


EEVTNW 

« Hide

References

« Hide 'large scale' references
[1]"The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence."
Langin T., Faugeron G., Goyon C., Nicolas A., Rossignol J.-L.
Gene 49:283-293(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Hell R.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M15675 Genomic DNA. Translation: AAA34775.1.
AJ001940 Genomic DNA. Translation: CAA05109.1.
Z71553 Genomic DNA. Translation: CAA96188.1.
BK006947 Genomic DNA. Translation: DAA10284.1.
PIRS63251.
RefSeqNP_014122.1. NM_001183115.1.

3D structure databases

ProteinModelPortalP08465.
SMRP08465. Positions 22-450.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35564. 11 interactions.
DIPDIP-3985N.
IntActP08465. 2 interactions.
MINTMINT-525497.
STRING4932.YNL277W.

Protein family/group databases

MEROPSS33.A41.

Proteomic databases

PaxDbP08465.

Protocols and materials databases

DNASU855444.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL277W; YNL277W; YNL277W.
GeneID855444.
KEGGsce:YNL277W.

Organism-specific databases

CYGDYNL277w.
SGDS000005221. MET2.

Phylogenomic databases

eggNOGCOG2021.
GeneTreeENSGT00530000067193.
HOGENOMHOG000246301.
KOK00641.
OMAEYFDANT.
OrthoDBEOG7M3J94.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-441.
YEAST:YNL277W-MONOMER.
UniPathwayUPA00051; UER00074.

Gene expression databases

GenevestigatorP08465.

Family and domain databases

InterProIPR000073. AB_hydrolase_1.
IPR008220. Homoserine_AcTrfase.
[Graphical view]
PfamPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFPIRSF000443. Homoser_Ac_trans. 1 hit.
TIGRFAMsTIGR01392. homoserO_Ac_trn. 1 hit.
ProtoNetSearch...

Other

NextBio979343.

Entry information

Entry nameMET2_YEAST
AccessionPrimary (citable) accession number: P08465
Secondary accession number(s): D6W0R8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways