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P08465

- MET2_YEAST

UniProt

P08465 - MET2_YEAST

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Protein

Homoserine O-acetyltransferase

Gene

MET2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei162 – 1621Sequence Analysis
Active sitei430 – 4301Sequence Analysis

GO - Molecular functioni

  1. homoserine O-acetyltransferase activity Source: SGD

GO - Biological processi

  1. homoserine metabolic process Source: SGD
  2. methionine biosynthetic process Source: SGD
  3. sulfur amino acid metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-441.
YEAST:YNL277W-MONOMER.
UniPathwayiUPA00051; UER00074.

Protein family/group databases

MEROPSiS33.A41.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine O-acetyltransferase (EC:2.3.1.31)
Alternative name(s):
Homoserine O-trans-acetylase
Gene namesi
Name:MET2
Ordered Locus Names:YNL277W
ORF Names:N0615
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL277w.
SGDiS000005221. MET2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Homoserine O-acetyltransferasePRO_0000155758Add
BLAST

Proteomic databases

MaxQBiP08465.
PaxDbiP08465.

Expressioni

Gene expression databases

GenevestigatoriP08465.

Interactioni

Protein-protein interaction databases

BioGridi35564. 12 interactions.
DIPiDIP-3985N.
IntActiP08465. 2 interactions.
MINTiMINT-525497.
STRINGi4932.YNL277W.

Structurei

3D structure databases

ProteinModelPortaliP08465.
SMRiP08465. Positions 28-249, 341-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. HTA family.Curated

Phylogenomic databases

eggNOGiCOG2021.
GeneTreeiENSGT00530000067193.
HOGENOMiHOG000246301.
InParanoidiP08465.
KOiK00641.
OMAiNRFDANC.
OrthoDBiEOG7M3J94.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR008220. Homoserine_AcTrfase.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000443. Homoser_Ac_trans. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
TIGRFAMsiTIGR01392. homoserO_Ac_trn. 1 hit.

Sequencei

Sequence statusi: Complete.

P08465 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHTLKSKTL QELDIEEIKE TNPLLKLVQG QRIVQVPELV LESGVVINNF
60 70 80 90 100
PIAYKTWGTL NEAGDNVLVI CHALTGSADV ADWWGPLLGN DLAFDPSRFF
110 120 130 140 150
IICLNSMGSP YGSFSPLTIN EETGVRYGPE FPLCTVRDDV RAHRIVLDSL
160 170 180 190 200
GVKSIACVIG GSMGGMLSLE WAAMYGKEYV KNMVALATSA RHSAWCISWS
210 220 230 240 250
EAQRQSIYSD PNYLDGYYPV EEQPVAGLSA ARMSALLTYR TRNSFENKFS
260 270 280 290 300
RRSPSIAQQQ KAQREETRKP STVSEHSLQI HNDGYKTKAS TAIAGISGQK
310 320 330 340 350
GQSVVSTASS SDSLNSSTSM TSVSSVTGEV KDIKPAQTYF SAQSYLRYQG
360 370 380 390 400
TKFINRFDAN CYIAITRKLD THDLARDRVD DITEVLSTIQ QPSLIIGIQS
410 420 430 440 450
DGLFTYSEQE FLAEHIPKSQ LEKIESPEGH DAFLLEFKLI NKLIVQFLKT
460 470 480
NCKAITDAAP RAWGGDVGND ETKTSVFGEA EEVTNW
Length:486
Mass (Da):53,659
Last modified:October 1, 1996 - v2
Checksum:iFD5E8B9D0CE8C707
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti429 – 48658GHDAF…EVTNW → ATMPSYWSLS(PubMed:3552887)CuratedAdd
BLAST
Sequence conflicti429 – 48658GHDAF…EVTNW → ATMPSYWSLS1 PublicationCuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15675 Genomic DNA. Translation: AAA34775.1.
AJ001940 Genomic DNA. Translation: CAA05109.1.
Z71553 Genomic DNA. Translation: CAA96188.1.
BK006947 Genomic DNA. Translation: DAA10284.1.
PIRiS63251.
RefSeqiNP_014122.1. NM_001183115.1.

Genome annotation databases

EnsemblFungiiYNL277W; YNL277W; YNL277W.
GeneIDi855444.
KEGGisce:YNL277W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15675 Genomic DNA. Translation: AAA34775.1 .
AJ001940 Genomic DNA. Translation: CAA05109.1 .
Z71553 Genomic DNA. Translation: CAA96188.1 .
BK006947 Genomic DNA. Translation: DAA10284.1 .
PIRi S63251.
RefSeqi NP_014122.1. NM_001183115.1.

3D structure databases

ProteinModelPortali P08465.
SMRi P08465. Positions 28-249, 341-448.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35564. 12 interactions.
DIPi DIP-3985N.
IntActi P08465. 2 interactions.
MINTi MINT-525497.
STRINGi 4932.YNL277W.

Protein family/group databases

MEROPSi S33.A41.

Proteomic databases

MaxQBi P08465.
PaxDbi P08465.

Protocols and materials databases

DNASUi 855444.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL277W ; YNL277W ; YNL277W .
GeneIDi 855444.
KEGGi sce:YNL277W.

Organism-specific databases

CYGDi YNL277w.
SGDi S000005221. MET2.

Phylogenomic databases

eggNOGi COG2021.
GeneTreei ENSGT00530000067193.
HOGENOMi HOG000246301.
InParanoidi P08465.
KOi K00641.
OMAi NRFDANC.
OrthoDBi EOG7M3J94.

Enzyme and pathway databases

UniPathwayi UPA00051 ; UER00074 .
BioCyci MetaCyc:MONOMER-441.
YEAST:YNL277W-MONOMER.

Miscellaneous databases

NextBioi 979343.

Gene expression databases

Genevestigatori P08465.

Family and domain databases

Gene3Di 3.40.50.1820. 2 hits.
InterProi IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR008220. Homoserine_AcTrfase.
[Graphical view ]
Pfami PF00561. Abhydrolase_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000443. Homoser_Ac_trans. 1 hit.
SUPFAMi SSF53474. SSF53474. 2 hits.
TIGRFAMsi TIGR01392. homoserO_Ac_trn. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence."
    Langin T., Faugeron G., Goyon C., Nicolas A., Rossignol J.-L.
    Gene 49:283-293(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Hell R.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMET2_YEAST
AccessioniPrimary (citable) accession number: P08465
Secondary accession number(s): D6W0R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2240 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3