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P08461 (ODP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

EC=2.3.1.12
Alternative name(s):
70 kDa mitochondrial autoantigen of primary biliary cirrhosis
Short name=PBC
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2
Short name=PDC-E2
Short name=PDCE2
Gene names
Name:Dlat
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length632 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.

Catalytic activity

Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine.

Cofactor

Binds 1 lipoyl cofactor covalently.

Subunit structure

Part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules. Interacts with PDK2 and PDK3 By similarity.

Subcellular location

Mitochondrion matrix.

Tissue specificity

Expressed in flagella of epididymal sperm. Ref.5

Involvement in disease

Primary biliary cirrhosis (PBC) is an autoimmune disease characterized by inflammatory obliteration of intra-hepatic bile duct, leading to liver cell damage and cirrhosis. The E2 component of pyruvate dehydrogenase complex is the autoantigen for PBC.

Sequence similarities

Belongs to the 2-oxoacid dehydrogenase family.

Contains 2 lipoyl-binding domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7777Mitochondrion Ref.2
Chain78 – 632555Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
PRO_0000162298

Regions

Domain83 – 15775Lipoyl-binding 1
Domain209 – 28375Lipoyl-binding 2
Region344 – 37532E3- and/or E1-component binding domain Potential
Region456 – 632177Catalytic By similarity
Region600 – 61112CoA-binding By similarity

Sites

Active site6051 Potential
Active site6091 Potential

Amino acid modifications

Modified residue2491N6-lipoyllysine
Modified residue4511N6-acetyllysine By similarity
Modified residue4581N6-succinyllysine By similarity
Modified residue5321N6-succinyllysine By similarity

Experimental info

Sequence conflict150 – 1523VGS → IGC in BAA01504. Ref.2
Sequence conflict163 – 1642DI → GP in BAA20956. Ref.3
Sequence conflict163 – 1642DI → GP in AAA41813. Ref.3
Sequence conflict601 – 63232LSCDH…VTMLL → HSAVIIELWMEQLEPSGLL in BAA20956. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P08461 [UniParc].

Last modified December 12, 2006. Version 3.
Checksum: 3F0EDBD44D93EB68

FASTA63267,166
        10         20         30         40         50         60 
MWRVCARRVQ SAVPRAGFRA RWATLKGPRT GPAAVRCGSG IPSYGVRSLC GWSYGSATVP 

        70         80         90        100        110        120 
RNRILQQLLG SPSRRSYSLP PHQKVPLPSL SPTMQAGTIA RWEKKEGEKI SEGDLIAEVE 

       130        140        150        160        170        180 
TDKATVGFES LEECYMAKIL VPEGTRDVPV GSIICITVEK PQDIEAFKNY TLDSATAATQ 

       190        200        210        220        230        240 
AAPAPAAAPA AAPAAPSASA PGSSYPVHMQ IVLPALSPTM TMGTVQRWEK KVGEKLSEGD 

       250        260        270        280        290        300 
LLAEIETDKA TIGFEVQEEG YLAKILVPEG TRDVPLGTPL CIIVEKQEDI AAFADYRPTE 

       310        320        330        340        350        360 
VTSLKPQAPP PVPPPVAAVP PIPQPLAPTP SAAPAGPKGR VFVSPLAKKL AAEKGIDLTQ 

       370        380        390        400        410        420 
VKGTGPEGRI IKKDIDSFVP TKAAPAAAAA APPGPRVAPT PAGVFIDIPI SNIRRVIAQR 

       430        440        450        460        470        480 
LMQSKQTIPH YYLSVDVNMG EVLLVRKELN KMLEGKGKIS VNDFIIKASA LACLKVPEAN 

       490        500        510        520        530        540 
SSWMDTVIRQ NHVVDVSVAV STPAGLITPI VFNAHIKGLE TIASDVVSLA SKAREGKLQP 

       550        560        570        580        590        600 
HEFQGGTFTI SNLGMFGIKN FSAIINPPQA CILAIGASED KLIPADNEKG FDVASVMSVT 

       610        620        630 
LSCDHRVVDG AVGAQWLAEF KKYLEKPVTM LL 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[2]"Molecular cloning of dihydrolipoamide acetyltransferase of the rat pyruvate dehydrogenase complex: sequence comparison and evolutionary relationship to other dihydrolipoamide acyltransferases."
Matuda S., Nakano K., Ohta S., Shimura M., Yamanaka T., Nakagawa S., Titani K., Miyata T.
Biochim. Biophys. Acta 1131:114-118(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-632, PROTEIN SEQUENCE OF 78-91.
Tissue: Heart.
[3]"Identification and specificity of a cDNA encoding the 70 kd mitochondrial antigen recognized in primary biliary cirrhosis."
Gershwin M.E., McKay I.R., Sturgess A., Coppel R.L.
J. Immunol. 138:3525-3531(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 163-632.
Tissue: Liver.
[4]Lubec G., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 397-414, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
[5]"Identification of novel immunodominant epididymal sperm proteins using combinatorial approach."
Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.
Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC107440 mRNA. Translation: AAI07441.1.
D10655 mRNA. Translation: BAA01504.1.
D00092 mRNA. Translation: BAA20956.1.
M16075 mRNA. Translation: AAA41813.1.
PIRS21766.
RefSeqNP_112287.1. NM_031025.1.
UniGeneRn.15413.

3D structure databases

ProteinModelPortalP08461.
SMRP08461. Positions 83-173, 204-305, 394-632.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP08461. 1 interaction.
STRING10116.ENSRNOP00000032890.

PTM databases

PhosphoSiteP08461.

2D gel databases

World-2DPAGE0004:P08461.

Proteomic databases

PaxDbP08461.
PRIDEP08461.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000032152; ENSRNOP00000032890; ENSRNOG00000009994.
GeneID81654.
KEGGrno:81654.
UCSCRGD:619859. rat.

Organism-specific databases

CTD1737.
RGD619859. Dlat.

Phylogenomic databases

eggNOGCOG0508.
GeneTreeENSGT00740000115255.
HOGENOMHOG000281566.
HOVERGENHBG005063.
InParanoidP08461.
KOK00627.
OMAPISNIRK.
OrthoDBEOG7K3TKW.
PhylomeDBP08461.
TreeFamTF106145.

Gene expression databases

GenevestigatorP08461.

Family and domain databases

Gene3D3.30.559.10. 1 hit.
4.10.320.10. 1 hit.
InterProIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR001078. 2-oxoacid_DH_actylTfrase.
IPR000089. Biotin_lipoyl.
IPR023213. CAT-like_dom.
IPR004167. E3-bd.
IPR006257. LAT1.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamPF00198. 2-oxoacid_dh. 1 hit.
PF00364. Biotin_lipoyl. 2 hits.
PF02817. E3_binding. 1 hit.
[Graphical view]
SUPFAMSSF47005. SSF47005. 1 hit.
SSF51230. SSF51230. 2 hits.
TIGRFAMsTIGR01349. PDHac_trf_mito. 1 hit.
PROSITEPS50968. BIOTINYL_LIPOYL. 2 hits.
PS00189. LIPOYL. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio615200.
PROP08461.

Entry information

Entry nameODP2_RAT
AccessionPrimary (citable) accession number: P08461
Secondary accession number(s): Q3B7V7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families