Nid1

Functionⁱ

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

GO - Biological processⁱ

cell-matrix adhesion Source: InterPro

Complete GO annotation...

Keywords - Biological processⁱ

Cell adhesion

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nidogen-1 Short name: NID-1 Alternative name(s): Entactin
Gene namesⁱ	Name:Nid1 Synonyms:Nid
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

RGDⁱ	3178. Nid1.

Subcellular locationⁱ

Secreted › extracellular space › extracellular matrix › basement membrane

GO - Cellular componentⁱ

basement membrane Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Basement membrane, Extracellular matrix, Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Chainⁱ	‹1 – ›324	›324	Nidogen-1		PRO_0000055634	Add BLAST

Amino acid modifications

Feature key	Position(s)	Length	Description
Glycosylationⁱ	97 – 97	1	N-linked (GlcNAc...)Sequence analysis
Modified residueⁱ	200 – 200	1	SulfotyrosineSequence analysis
Modified residueⁱ	205 – 205	1	SulfotyrosineSequence analysis
Disulfide bondⁱ	299 ↔ 312		PROSITE-ProRule annotation Manual assertion according to rulesⁱ PROSITE-ProRule:PRU00076
Disulfide bondⁱ	306 ↔ 321		PROSITE-ProRule annotation Manual assertion according to rulesⁱ PROSITE-ProRule:PRU00076

Post-translational modificationⁱ

N- and O-glycosylated.

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbⁱ	P08460.
PeptideAtlasⁱ	P08460.
PRIDEⁱ	P08460.

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSRNOG00000002461.

Bgeeⁱ

ENSRNOG00000002461.

Interactionⁱ

Subunit structureⁱ

Interacts with FBLN1 and LGALS3BP. Interacts with PLXDC1 (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Plxdc1	Q91ZV7	2	EBI-8280787,EBI-8280807	From a different organism.

With

Entry

#Exp.

IntAct

Notes

Plxdc1

Q91ZV7

2

EBI-8280787,EBI-8280807

From a different organism.

Protein-protein interaction databases

IntActⁱ	P08460. 1 interaction.
MINTⁱ	MINT-1892021.
STRINGⁱ	10116.ENSRNOP00000003349.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P08460.
SMRⁱ	P08460. Positions 298-324.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Domainⁱ	16 – 178	163	NIDOPROSITE-ProRule annotation Manual assertion according to rulesⁱ PROSITE-ProRule:PRU00570			Add BLAST
Domainⁱ	295 – ›324	›30	EGF-likePROSITE-ProRule annotation Manual assertion according to rulesⁱ PROSITE-ProRule:PRU00076			Add BLAST

Sequence similaritiesⁱ

Contains at least 1 EGF-like domain.Curated

Contains 1 NIDO domain.PROSITE-ProRule annotation

Keywords - Domainⁱ

EGF-like domain

Phylogenomic databases

eggNOGⁱ	KOG1214. Eukaryota. ENOG410XR8N. LUCA.
HOGENOMⁱ	HOG000072712.
InParanoidⁱ	P08460.
PhylomeDBⁱ	P08460.

Family and domain databases

InterProⁱ	IPR003886. NIDO_dom. [Graphical view]
Pfamⁱ	PF06119. NIDO. 1 hit. [Graphical view]
SMARTⁱ	SM00539. NIDO. 1 hit. [Graphical view]
PROSITEⁱ	PS00010. ASX_HYDROXYL. 1 hit. PS51220. NIDO. 1 hit. [Graphical view]

Sequenceⁱ

Sequence statusⁱ: Fragment.

P08460-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
EFHPGTFPPS FGSVAPFLAD LDTTDGLGNV YYREDLSPFI IQMAAEYVQR 
        60         70         80         90        100
GFPEVSFQPT SVVVVTWESM APYGGPSGSL VEEGKRNTFQ AVLASSNSSS 
       110        120        130        140        150
YAIFLYPDDG LQFFTTFSKK DENQVPAVVG FSKGLEGFLW KSNGAYNIFA 
       160        170        180        190        200
NDRESIENLA KSSNAGHQGV WVFEIGSPAT AKGVVPADVN LDVDDDGADY 
       210        220        230        240        250
EDEDYDLQTS HLGLEDVATQ PFPSHSPRRG YPDPHNVPRT LAPSYEATER 
       260        270        280        290        300
PHGIPTERTK SFQLPVERFP QKHPQVIDVD EVEETGVVFS YNTGSQQTCA 
       310        320 
NNRHQCSVHA ECRDYATGFC CRCV

Length:324

Mass (Da):35,661

Last modified:May 1, 1992 - v2

Checksum:ⁱCBDEAD3337019027

GO

Experimental Info

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Non-terminal residueⁱ	1 – 1	1
Non-terminal residueⁱ	324 – 324	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M15797 mRNA. Translation: AAA41120.1. Sequence problems. AJ224450 mRNA. Translation: CAA11963.1.
PIRⁱ	S14828.
UniGeneⁱ	Rn.105658.

Genome annotation databases

UCSCⁱ	RGD:3178. rat.

UCSCⁱ

RGD:3178. rat.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M15797 mRNA. Translation: AAA41120.1. Sequence problems. AJ224450 mRNA. Translation: CAA11963.1.
PIRⁱ	S14828.
UniGeneⁱ	Rn.105658.

3D structure databases

ProteinModelPortalⁱ	P08460.
SMRⁱ	P08460. Positions 298-324.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P08460. 1 interaction.
MINTⁱ	MINT-1892021.
STRINGⁱ	10116.ENSRNOP00000003349.

Proteomic databases

PaxDbⁱ	P08460.
PeptideAtlasⁱ	P08460.
PRIDEⁱ	P08460.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

UCSCⁱ	RGD:3178. rat.

UCSCⁱ

RGD:3178. rat.

Organism-specific databases

RGDⁱ	3178. Nid1.

Phylogenomic databases

eggNOGⁱ	KOG1214. Eukaryota. ENOG410XR8N. LUCA.
HOGENOMⁱ	HOG000072712.
InParanoidⁱ	P08460.
PhylomeDBⁱ	P08460.

Gene expression databases

Bgeeⁱ	ENSRNOG00000002461.

Bgeeⁱ

ENSRNOG00000002461.

Family and domain databases

InterProⁱ	IPR003886. NIDO_dom. [Graphical view]
Pfamⁱ	PF06119. NIDO. 1 hit. [Graphical view]
SMARTⁱ	SM00539. NIDO. 1 hit. [Graphical view]
PROSITEⁱ	PS00010. ASX_HYDROXYL. 1 hit. PS51220. NIDO. 1 hit. [Graphical view]
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ

NID1_RAT

Accessionⁱ

Primary (citable) accession number: P08460
Secondary accession number(s): Q712L9

Entry historyⁱ

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	May 1, 1992
Last modified:	September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry statusⁱ

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P08460 (NID1_RAT)

UniProt

P08460 - NID1_RAT

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Nidogen-1

Select a section on the left to see content.

<p>Provides any useful information about the protein, mostly biological knowledge.</p><p><a href='../manual/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>Provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.</p><p><a href='../manual/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>Provides information on the location and the topology of the mature protein in the cell.</p><p><a href='../manual/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>Provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.</p><p><a href='../manual/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>Provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.</p><p><a href='../manual/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>Provides information on the tertiary and secondary structure of a protein.</p><p><a href='../manual/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>Provides information on sequence similarities with other proteins and the domain(s) present in a protein.</p><p><a href='../manual/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>Provides information about the sequence similarity with other proteins.</p><p><a href='../manual/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>Displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.</p><p><a href='../manual/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>Is used to point to information related to entries and found in data collections other than UniProtKB.</p><p><a href='../manual/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

<p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>Contains any relevant information that doesn’t fit in any other defined sections</p><p><a href='../manual/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ