Nid1

Functionⁱ

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell-matrix adhesion
Source: RGD
Inferred from sequence orthologyⁱ
- 12243745
extracellular matrix organization
Source: RGD
Inferred from sequence orthologyⁱ
- 18757743
glomerular basement membrane development
Source: RGD
Inferred from sequence orthologyⁱ
- 14566019
positive regulation of cell-substrate adhesion
Source: RGD
Inferred from sequence orthologyⁱ
- 18757743

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell adhesion

Biological process

Cell adhesion

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nidogen-1 Short name: NID-1 Alternative name(s): Entactin
Gene namesⁱ	Name:Nid1 Synonyms:Nid
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	3178. Nid1.

RGDⁱ

3178. Nid1.

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Basement membrane, Extracellular matrix, Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000055634	‹1 – ›324	Nidogen-1Add BLAST		›324

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	97	N-linked (GlcNAc...) asparagineSequence analysis	1
Modified residueⁱ	200	SulfotyrosineSequence analysis	1
Modified residueⁱ	205	SulfotyrosineSequence analysis	1
Disulfide bondⁱ	299 ↔ 312	PROSITE-ProRule annotation
Disulfide bondⁱ	306 ↔ 321	PROSITE-ProRule annotation

Post-translational modificationⁱ

N- and O-glycosylated.

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbⁱ	P08460.
PeptideAtlas More...PeptideAtlasⁱ	P08460.
PRIDEⁱ	P08460.

Interactionⁱ

Subunit structureⁱ

Interacts with FBLN1 and LGALS3BP. Interacts with PLXDC1 (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Plxdc1	Q91ZV7	2	EBI-8280787,EBI-8280807	From Mus musculus.

With

Entry

#Exp.

IntAct

Notes

Plxdc1

Q91ZV7

2

EBI-8280787,EBI-8280807

From Mus musculus.

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActⁱ	P08460. 1 interactor.
Molecular INTeraction database More...MINTⁱ	P08460.
STRINGⁱ	10116.ENSRNOP00000003349.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P08460.
SMRⁱ	P08460.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	16 – 178	NIDOPROSITE-ProRule annotationAdd BLAST		163
Domainⁱ	295 – ›324	EGF-likePROSITE-ProRule annotationAdd BLAST		›30

Keywords - Domainⁱ

EGF-like domain

Phylogenomic databases

eggNOGⁱ	KOG1214. Eukaryota. ENOG410XR8N. LUCA.
HOGENOMⁱ	HOG000072712.
InParanoidⁱ	P08460.
PhylomeDBⁱ	P08460.

Family and domain databases

InterProⁱ	View protein in InterPro IPR003886. NIDO_dom.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06119. NIDO. 1 hit.
SMARTⁱ	View protein in SMART SM00539. NIDO. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00010. ASX_HYDROXYL. 1 hit. PS51220. NIDO. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Fragment.

P08460-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
EFHPGTFPPS FGSVAPFLAD LDTTDGLGNV YYREDLSPFI IQMAAEYVQR 
        60         70         80         90        100
GFPEVSFQPT SVVVVTWESM APYGGPSGSL VEEGKRNTFQ AVLASSNSSS 
       110        120        130        140        150
YAIFLYPDDG LQFFTTFSKK DENQVPAVVG FSKGLEGFLW KSNGAYNIFA 
       160        170        180        190        200
NDRESIENLA KSSNAGHQGV WVFEIGSPAT AKGVVPADVN LDVDDDGADY 
       210        220        230        240        250
EDEDYDLQTS HLGLEDVATQ PFPSHSPRRG YPDPHNVPRT LAPSYEATER 
       260        270        280        290        300
PHGIPTERTK SFQLPVERFP QKHPQVIDVD EVEETGVVFS YNTGSQQTCA 
       310        320 
NNRHQCSVHA ECRDYATGFC CRCV

Length:324

Mass (Da):35,661

Last modified:May 1, 1992 - v2

Checksum:ⁱCBDEAD3337019027

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Non-terminal residueⁱ	1			1
Non-terminal residueⁱ	324			1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M15797 mRNA. Translation: AAA41120.1. Sequence problems. AJ224450 mRNA. Translation: CAA11963.1.
PIRⁱ	S14828.
UniGeneⁱ	Rn.105658.

Genome annotation databases

UCSC genome browser More...UCSCⁱ	RGD:3178. rat.

UCSCⁱ

RGD:3178. rat.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P08460	F1LM84	Rattus norvegicus (Rat)	324	UniRef100_P08460	Cluster: Nidogen-1 (Fragment)	2

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P08460	P10493 UPI0000111A96 UPI00001B94C4 A0A1U7QPE1 UPI0004541513 UPI0007DA76CB UPI0007DAA0C0 UPI000A3069D4 UPI00038BC388 UPI000B4F4175 +4	Mus musculus (Mouse) Mesocricetus auratus (Golden hamster) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Mus pahari (Gairdner's shrew-mouse) (Coelomys pahari) Microtus ochrogaster (Prairie vole) Meriones unguiculatus (Mongolian jird) (Mongolian gerbil) Peromyscus maniculatus bairdii (Prairie deer mouse) Rattus norvegicus (Rat) Mus caroli (Ryukyu mouse) (Ricefield mouse)	1,245	UniRef90_P10493	Cluster: Nidogen-1	15

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P08460	P10493 UPI0000111A96 UPI00001B94C4 A0A1U7QPE1 UPI000A3069D4 UPI0007DAA0C0 UPI0004541513 UPI0007DA76CB UPI00038BC388 UPI000B4F4175 +41	Mus musculus (Mouse) Mesocricetus auratus (Golden hamster) Mus pahari (Gairdner's shrew-mouse) (Coelomys pahari) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) Microtus ochrogaster (Prairie vole) Meriones unguiculatus (Mongolian jird) (Mongolian gerbil) Peromyscus maniculatus bairdii (Prairie deer mouse) Rattus norvegicus (Rat) Mus caroli (Ryukyu mouse) (Ricefield mouse) Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus) And more	1,245	UniRef50_P10493	Cluster: Nidogen-1	52

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M15797 mRNA. Translation: AAA41120.1. Sequence problems. AJ224450 mRNA. Translation: CAA11963.1.
PIRⁱ	S14828.
UniGeneⁱ	Rn.105658.

3D structure databases

ProteinModelPortalⁱ	P08460.
SMRⁱ	P08460.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	P08460. 1 interactor.
Molecular INTeraction database More...MINTⁱ	P08460.
STRINGⁱ	10116.ENSRNOP00000003349.

Proteomic databases

PaxDbⁱ	P08460.
PeptideAtlas More...PeptideAtlasⁱ	P08460.
PRIDEⁱ	P08460.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

UCSC genome browser More...UCSCⁱ	RGD:3178. rat.

UCSCⁱ

RGD:3178. rat.

Organism-specific databases

Rat genome database More...RGDⁱ	3178. Nid1.

RGDⁱ

3178. Nid1.

Phylogenomic databases

eggNOGⁱ	KOG1214. Eukaryota. ENOG410XR8N. LUCA.
HOGENOMⁱ	HOG000072712.
InParanoidⁱ	P08460.
PhylomeDBⁱ	P08460.

Family and domain databases

InterProⁱ	View protein in InterPro IPR003886. NIDO_dom.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06119. NIDO. 1 hit.
SMARTⁱ	View protein in SMART SM00539. NIDO. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00010. ASX_HYDROXYL. 1 hit. PS51220. NIDO. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NID1_RAT
Accessionⁱ	P08460Primary (citable) accession number: P08460 Secondary accession number(s): Q712L9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	May 1, 1992
	Last modified:	February 28, 2018
	This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P08460 (NID1_RAT)

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Nidogen-1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

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<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ