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Protein

Sporulation-specific protein 1

Gene

SPS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase required for spore wall development.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei47 – 471ATPPROSITE-ProRule annotation
Active sitei141 – 1411Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 329ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • ascospore formation Source: SGD
  • ascospore wall assembly Source: SGD
  • protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Meiosis, Sporulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30039-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-75153. Apoptotic execution phase.

Names & Taxonomyi

Protein namesi
Recommended name:
Sporulation-specific protein 1 (EC:2.7.11.1)
Gene namesi
Name:SPS1
Ordered Locus Names:YDR523C
ORF Names:D9719.27
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR523C.
SGDiS000002931. SPS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Sporulation-specific protein 1PRO_0000086679Add
BLAST

PTM databases

iPTMnetiP08458.

Interactioni

Protein-protein interaction databases

BioGridi32573. 85 interactions.
DIPiDIP-6598N.
IntActiP08458. 4 interactions.
MINTiMINT-673664.

Structurei

3D structure databases

ProteinModelPortaliP08458.
SMRiP08458. Positions 10-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 272255Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00810000125395.
HOGENOMiHOG000234203.
InParanoidiP08458.
KOiK12771.
OMAiQEIFFLA.
OrthoDBiEOG7DFXMX.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESKEISIRS RTPPSKLYSI QSCIGRGNFG DVYKAVDRVT QEIVAIKVVN
60 70 80 90 100
LEHSDEDIEL LAQEIFFLAE LKSPLITNYI ATMLEDVSMW IVMEYCGGGS
110 120 130 140 150
CSDLLKRSYV NGLPEEKVSF IIHEVTLGLK YLHEQRKIHR DIKAANILLN
160 170 180 190 200
EEGMVKLGDF GVSGHIRSTL KRDTFVGTPY WMAPEVVCCE VDGYNEKADI
210 220 230 240 250
WSLGITTYEL LKGLPPLSKY DPMKVMTNLP KRKPPKLQGP FSDAAKDFVA
260 270 280 290 300
GCLVKTPADR PSAYNLLSFE FVKNITITNL KSDVDLIKQK KVQERYTKVP
310 320 330 340 350
KYPLQNRLYK NSNTVRGKEF WNFESTRLST TQISKEELSP ITQDSPTSSL
360 370 380 390 400
NMESPYLLHG QTVTPITNPS SSSFRKCTQP VFELDSGMDI DSGCPNAQAE
410 420 430 440 450
TEIVPLSNHN KKHKKNDIQA LKIEKFDYLK NIVSHILNRM YDRARDDETR
460 470 480 490
KYVNEMLKQF IKTEANVPGF NEVFIEEISL RIEAIKKGFV
Length:490
Mass (Da):55,704
Last modified:November 1, 1995 - v2
Checksum:i6143055E85BAF4FF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti454 – 4541N → NVN (PubMed:9169867).Curated
Sequence conflicti469 – 4691G → R in AAA35079 (PubMed:9169867).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13018 Genomic DNA. Translation: AAA64833.1.
U33057 Genomic DNA. Translation: AAB64963.1.
M13629 Genomic DNA. Translation: AAA35079.1.
BK006938 Genomic DNA. Translation: DAA12353.1.
PIRiA55162. S47946.
RefSeqiNP_010811.1. NM_001180831.1.

Genome annotation databases

EnsemblFungiiYDR523C; YDR523C; YDR523C.
GeneIDi852135.
KEGGisce:YDR523C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13018 Genomic DNA. Translation: AAA64833.1.
U33057 Genomic DNA. Translation: AAB64963.1.
M13629 Genomic DNA. Translation: AAA35079.1.
BK006938 Genomic DNA. Translation: DAA12353.1.
PIRiA55162. S47946.
RefSeqiNP_010811.1. NM_001180831.1.

3D structure databases

ProteinModelPortaliP08458.
SMRiP08458. Positions 10-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32573. 85 interactions.
DIPiDIP-6598N.
IntActiP08458. 4 interactions.
MINTiMINT-673664.

PTM databases

iPTMnetiP08458.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR523C; YDR523C; YDR523C.
GeneIDi852135.
KEGGisce:YDR523C.

Organism-specific databases

EuPathDBiFungiDB:YDR523C.
SGDiS000002931. SPS1.

Phylogenomic databases

GeneTreeiENSGT00810000125395.
HOGENOMiHOG000234203.
InParanoidiP08458.
KOiK12771.
OMAiQEIFFLA.
OrthoDBiEOG7DFXMX.

Enzyme and pathway databases

BioCyciYEAST:G3O-30039-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-75153. Apoptotic execution phase.

Miscellaneous databases

NextBioi970540.
PROiP08458.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutation of the SPS1-encoded protein kinase of Saccharomyces cerevisiae leads to defects in transcription and morphology during spore formation."
    Friesen H., Lunz R., Doyle S., Segall J.
    Genes Dev. 8:2162-2175(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Characterization and mutational analysis of a cluster of three genes expressed preferentially during sporulation of Saccharomyces cerevisiae."
    Percival-Smith A., Segall J.
    Mol. Cell. Biol. 6:2443-2451(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 396-490.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSPS1_YEAST
AccessioniPrimary (citable) accession number: P08458
Secondary accession number(s): D6VTE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.