P08456 (PSS_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 121.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: CDP-diacylglycerol--serine O-phosphatidyltransferase EC=2.7.8.8 Alternative name(s): Phosphatidylserine synthase | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 276 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine. |
| Pathway | |
| Subcellular location | Microsome. Endoplasmic reticulum. Mitochondrion outer membrane; Peripheral membrane protein. |
| Sequence similarities | Belongs to the CDP-alcohol phosphatidyltransferase class-I family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Phospholipid biosynthesis |
| Cellular component | Endoplasmic reticulum Membrane Microsome Mitochondrion Mitochondrion outer membrane |
| Molecular function | Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | CDP-diacylglycerol metabolic process Inferred from electronic annotation. Source: InterPro phosphatidylserine biosynthetic processInferred from direct assay. Source: SGD |
| Cellular component | endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from direct assay. Source: SGD microsomeInferred from direct assay. Source: SGD mitochondrial outer membraneInferred from direct assay. Source: SGD |
| Molecular function | CDP-diacylglycerol-serine O-phosphatidyltransferase activity Inferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.2 | ||||||
| Chain | 2 – 276 | 275 | CDP-diacylglycerol--serine O-phosphatidyltransferase | PRO_0000056801 | |||||
Amino acid modifications | |||||||||
| Modified residue | 4 | 1 | Phosphoserine Ref.8 Ref.10 | ||||||
| Modified residue | 32 | 1 | Phosphotyrosine Ref.10 | ||||||
| Modified residue | 34 | 1 | Phosphoserine Ref.5 Ref.6 Ref.8 Ref.10 | ||||||
| Modified residue | 42 | 1 | Phosphoserine Ref.5 Ref.8 | ||||||
| Modified residue | 46 | 1 | Phosphoserine Ref.8 Ref.9 Ref.10 | ||||||
| Modified residue | 47 | 1 | Phosphoserine Ref.7 Ref.8 Ref.9 Ref.10 | ||||||
| Modified residue | 50 | 1 | Phosphoserine Ref.8 Ref.10 | ||||||
| Modified residue | 53 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 54 | 1 | Phosphothreonine Ref.10 | ||||||
Experimental info | |||||||||
| Sequence conflict | 123 | 1 | G → A in BAA00121. Ref.2 | ||||||
| Sequence conflict | 191 | 1 | R → T in BAA00121. Ref.2 | ||||||
| Sequence conflict | 276 | 1 | P → A in BAA00121. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence and characterization of the yeast PSS gene encoding phosphatidylserine synthase." Nikawa J., Tsukagoschi Y., Kodaki T., Yamashita S. Eur. J. Biochem. 167:7-12(1987) [PubMed: 3040403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Primary structure and product characterization of the Saccharomyces cerevisiae CHO1 gene that encodes phosphatidylserine synthase." Kiyono K., Miura K., Kushima Y., Hikiji T., Fukushima M., Shibuya I., Ohta A. J. Biochem. 102:1089-1100(1987) [PubMed: 2830250] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W.  Davis R.W.Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [5] | "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34 AND SER-42, MASS SPECTROMETRY. Strain: 2124. |
| [6] | "A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, MASS SPECTROMETRY. Strain: SUB592. |
| [7] | "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, MASS SPECTROMETRY. Strain: YAL6B. |
| [8] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-34; SER-42; SER-46; SER-47 AND SER-50, MASS SPECTROMETRY. Strain: ADR376. |
| [9] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-47, MASS SPECTROMETRY. |
| [10] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; TYR-32; SER-34; SER-46; SER-47; SER-50; THR-53 AND THR-54, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X05944 Genomic DNA. Translation: CAA29376.1. D00171 Genomic DNA. Translation: BAA00121.1. U18778 Genomic DNA. Translation: AAB64559.1. BK006939 Genomic DNA. Translation: DAA07679.1. |
| PIR | S00080. |
| RefSeq | NP_010943.1. NM_001178917.1. |
3D structure databases | |
| ProteinModelPortal | P08456. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-5331N. |
| IntAct | P08456. 102 interactions. |
| MINT | MINT-521670. |
| STRING | P08456. |
Proteomic databases | |
| PeptideAtlas | P08456. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YER026C; YER026C; YER026C. |
| GeneID | 856748. |
| KEGG | sce:YER026C. |
| NMPDR | fig|4932.3.peg.2004. |
Organism-specific databases | |
| SGD | S000000828. CHO1. |
Phylogenomic databases | |
| eggNOG | fuNOG06964. |
| GeneTree | EFGT00050000004312. |
| HOGENOM | HBG735258. |
| OMA | FYSIVSC. |
| OrthoDB | EOG4R7ZKX. |
Enzyme and pathway databases | |
| BRENDA | 2.7.8.8. 984. |
Gene expression databases | |
| ArrayExpress | P08456. |
| Genevestigator | P08456. |
| GermOnline | YER026C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR004533. CDP-diaglyc--ser_O-PTrfase. IPR016271. CDP-diaglyc--ser_O-PTrfase_fun. IPR000462. CDP-OH_P_trans. [Graphical view] |
| KO | K00998. |
| Pfam | PF01066. CDP-OH_P_transf. 1 hit. [Graphical view] |
| PIRSF | PIRSF000852. Phosphatidylserine_synth_fun. 1 hit. |
| TIGRFAMs | TIGR00473. PssA. 1 hit. |
| PROSITE | PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 982893. |
Entry information
| Entry name | PSS_YEAST | ||||||||
| Accession | Primary (citable) accession number: P08456 Secondary accession number(s): D3DLS5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |