P08456 (PSS_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 135.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: CDP-diacylglycerol--serine O-phosphatidyltransferase EC=2.7.8.8 Alternative name(s): Phosphatidylserine synthase | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›  |
Protein attributes
| Sequence length | 276 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine. |
| Pathway | |
| Subcellular location | Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. |
| Sequence similarities | Belongs to the CDP-alcohol phosphatidyltransferase class-I family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism |
| Cellular component | Endoplasmic reticulum Membrane Microsome Mitochondrion Mitochondrion outer membrane |
| Domain | Transmembrane Transmembrane helix |
| Molecular function | Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | phosphatidylethanolamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway phosphatidylserine biosynthetic processInferred from direct assay PubMed 6088519. Source: SGD |
| Cellular_component | integral to membrane Inferred from direct assay PubMed 6274497. Source: SGD mitochondrial outer membraneInferred from direct assay PubMed 3005242. Source: SGD |
| Molecular_function | CDP-diacylglycerol-serine O-phosphatidyltransferase activity Inferred from direct assay PubMed 6088519. Source: SGD |
| Complete GO annotation... | |
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.2 | ||||||
| Chain | 2 – 276 | 275 | CDP-diacylglycerol--serine O-phosphatidyltransferase | PRO_0000056801 | |||||
Regions | |||||||||
| Transmembrane | 82 – 102 | 21 | Helical; Potential | ||||||
| Transmembrane | 163 – 183 | 21 | Helical; Potential | ||||||
| Transmembrane | 210 – 230 | 21 | Helical; Potential | ||||||
| Transmembrane | 248 – 268 | 21 | Helical; Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 4 | 1 | Phosphoserine Ref.8 Ref.10 | ||||||
| Modified residue | 32 | 1 | Phosphotyrosine Ref.10 | ||||||
| Modified residue | 34 | 1 | Phosphoserine Ref.5 Ref.6 Ref.8 Ref.10 | ||||||
| Modified residue | 42 | 1 | Phosphoserine Ref.5 Ref.8 | ||||||
| Modified residue | 46 | 1 | Phosphoserine Ref.8 Ref.9 Ref.10 | ||||||
| Modified residue | 47 | 1 | Phosphoserine Ref.7 Ref.8 Ref.9 Ref.10 | ||||||
| Modified residue | 50 | 1 | Phosphoserine Ref.8 Ref.10 | ||||||
| Modified residue | 53 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 54 | 1 | Phosphothreonine Ref.10 | ||||||
Experimental info | |||||||||
| Sequence conflict | 123 | 1 | G → A in BAA00121. Ref.2 | ||||||
| Sequence conflict | 191 | 1 | R → T in BAA00121. Ref.2 | ||||||
| Sequence conflict | 276 | 1 | P → A in BAA00121. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence and characterization of the yeast PSS gene encoding phosphatidylserine synthase." Nikawa J., Tsukagoschi Y., Kodaki T., Yamashita S. Eur. J. Biochem. 167:7-12(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Primary structure and product characterization of the Saccharomyces cerevisiae CHO1 gene that encodes phosphatidylserine synthase." Kiyono K., Miura K., Kushima Y., Hikiji T., Fukushima M., Shibuya I., Ohta A. J. Biochem. 102:1089-1100(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W.  Davis R.W.Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [5] | "Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34 AND SER-42, MASS SPECTROMETRY. Strain: 2124. |
| [6] | "A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, MASS SPECTROMETRY. Strain: SUB592. |
| [7] | "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, MASS SPECTROMETRY. Strain: YAL6B. |
| [8] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-34; SER-42; SER-46; SER-47 AND SER-50, MASS SPECTROMETRY. Strain: ADR376. |
| [9] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-47, MASS SPECTROMETRY. |
| [10] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; TYR-32; SER-34; SER-46; SER-47; SER-50; THR-53 AND THR-54, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X05944 Genomic DNA. Translation: CAA29376.1. D00171 Genomic DNA. Translation: BAA00121.1. U18778 Genomic DNA. Translation: AAB64559.1. BK006939 Genomic DNA. Translation: DAA07679.1. |
| PIR | S00080. |
| RefSeq | NP_010943.3. NM_001178917.3. |
3D structure databases | |
| ProteinModelPortal | P08456. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-5331N. |
| IntAct | P08456. 1 interaction. |
| MINT | MINT-521670. |
| STRING | 4932.YER026C. |
Proteomic databases | |
| PaxDb | P08456. |
| PeptideAtlas | P08456. |
Protocols and materials databases | |
| DNASU | 856748. |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YER026C; YER026C; YER026C. |
| GeneID | 856748. |
| KEGG | sce:YER026C. |
Organism-specific databases | |
| SGD | S000000828. CHO1. |
Phylogenomic databases | |
| eggNOG | COG1183. |
| HOGENOM | HOG000229371. |
| KO | K00998. |
| OMA | RGFHLAD. |
| OrthoDB | EOG4R7ZKX. |
Enzyme and pathway databases | |
| BioCyc | YEAST:YER026C-MONOMER. |
| BRENDA | 2.7.8.8. 984. |
| UniPathway | UPA00558; UER00615. |
Gene expression databases | |
| Genevestigator | P08456. |
| GermOnline | YER026C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR004533. CDP-diaglyc--ser_O-PTrfase. IPR016271. CDP-diaglyc--ser_O-PTrfase_fun. IPR000462. CDP-OH_P_trans. [Graphical view] |
| Pfam | PF01066. CDP-OH_P_transf. 1 hit. [Graphical view] |
| PIRSF | PIRSF000852. Phosphatidylserine_synth_fun. 1 hit. |
| TIGRFAMs | TIGR00473. pssA. 1 hit. |
| PROSITE | PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 982893. |
Entry information
| Entry name | PSS_YEAST | ||||||||
| Accession | Primary (citable) accession number: P08456 Secondary accession number(s): D3DLS5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |