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P08456

- PSS_YEAST

UniProt

P08456 - PSS_YEAST

Protein

CDP-diacylglycerol--serine O-phosphatidyltransferase

Gene

CHO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine.

    Pathwayi

    GO - Molecular functioni

    1. CDP-diacylglycerol-serine O-phosphatidyltransferase activity Source: SGD

    GO - Biological processi

    1. phosphatidylethanolamine biosynthetic process Source: UniProtKB-UniPathway
    2. phosphatidylserine biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    BioCyciYEAST:YER026C-MONOMER.
    BRENDAi2.7.8.8. 984.
    UniPathwayiUPA00558; UER00615.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CDP-diacylglycerol--serine O-phosphatidyltransferase (EC:2.7.8.8)
    Alternative name(s):
    Phosphatidylserine synthase
    Gene namesi
    Name:CHO1
    Synonyms:PSS, PSS1
    Ordered Locus Names:YER026C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000828. CHO1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: SGD
    2. mitochondrial outer membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 276275CDP-diacylglycerol--serine O-phosphatidyltransferasePRO_0000056801Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei4 – 41Phosphoserine3 Publications
    Modified residuei34 – 341Phosphoserine3 Publications
    Modified residuei42 – 421Phosphoserine2 Publications
    Modified residuei46 – 461Phosphoserine2 Publications
    Modified residuei47 – 471Phosphoserine2 Publications
    Modified residuei50 – 501Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP08456.
    PeptideAtlasiP08456.

    Expressioni

    Gene expression databases

    GenevestigatoriP08456.

    Interactioni

    Protein-protein interaction databases

    BioGridi36761. 39 interactions.
    DIPiDIP-5331N.
    IntActiP08456. 7 interactions.
    MINTiMINT-521670.
    STRINGi4932.YER026C.

    Structurei

    3D structure databases

    ProteinModelPortaliP08456.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei82 – 10221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei163 – 18321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei210 – 23021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei248 – 26821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1183.
    HOGENOMiHOG000229371.
    KOiK17103.
    OMAiFNISANK.
    OrthoDBiEOG7W15FB.

    Family and domain databases

    InterProiIPR004533. CDP-diaglyc--ser_O-PTrfase.
    IPR016271. CDP-diaglyc--ser_O-PTrfase_fun.
    IPR000462. CDP-OH_P_trans.
    [Graphical view]
    PfamiPF01066. CDP-OH_P_transf. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000852. Phosphatidylserine_synth_fun. 1 hit.
    TIGRFAMsiTIGR00473. pssA. 1 hit.
    PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08456-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVESDEDFAP QEFPHTDTDV IVNEHRDEND GYASDEVGGT LSRRASSIFS    50
    INTTPLAPPN ATDIQKFTSD EHHFSMMRNL HMADYITMLN GFSGFYSIVS 100
    CLRFTLTGKP HYVQRAHFFI LLGMCFDFLD GRVARLRNRS SLMGQELDSL 150
    ADLVSFGVAP AAIAFAIGFQ TTFDVMILSF FVLCGLARLA RFNVTVAQLP 200
    KDSSTGKSKY FEGLPMPTTL ALVLGMAYCV RKGLIFDNIP FGIFREDQIL 250
    EFHPIILVFF IHGCGMISKS LKIPKP 276
    Length:276
    Mass (Da):30,805
    Last modified:January 23, 2007 - v3
    Checksum:iE52B1FC836B67D24
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti123 – 1231G → A in BAA00121. (PubMed:2830250)Curated
    Sequence conflicti191 – 1911R → T in BAA00121. (PubMed:2830250)Curated
    Sequence conflicti276 – 2761P → A in BAA00121. (PubMed:2830250)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05944 Genomic DNA. Translation: CAA29376.1.
    D00171 Genomic DNA. Translation: BAA00121.1.
    U18778 Genomic DNA. Translation: AAB64559.1.
    BK006939 Genomic DNA. Translation: DAA07679.1.
    PIRiS00080.
    RefSeqiNP_010943.3. NM_001178917.3.

    Genome annotation databases

    EnsemblFungiiYER026C; YER026C; YER026C.
    GeneIDi856748.
    KEGGisce:YER026C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05944 Genomic DNA. Translation: CAA29376.1 .
    D00171 Genomic DNA. Translation: BAA00121.1 .
    U18778 Genomic DNA. Translation: AAB64559.1 .
    BK006939 Genomic DNA. Translation: DAA07679.1 .
    PIRi S00080.
    RefSeqi NP_010943.3. NM_001178917.3.

    3D structure databases

    ProteinModelPortali P08456.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36761. 39 interactions.
    DIPi DIP-5331N.
    IntActi P08456. 7 interactions.
    MINTi MINT-521670.
    STRINGi 4932.YER026C.

    Proteomic databases

    PaxDbi P08456.
    PeptideAtlasi P08456.

    Protocols and materials databases

    DNASUi 856748.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER026C ; YER026C ; YER026C .
    GeneIDi 856748.
    KEGGi sce:YER026C.

    Organism-specific databases

    SGDi S000000828. CHO1.

    Phylogenomic databases

    eggNOGi COG1183.
    HOGENOMi HOG000229371.
    KOi K17103.
    OMAi FNISANK.
    OrthoDBi EOG7W15FB.

    Enzyme and pathway databases

    UniPathwayi UPA00558 ; UER00615 .
    BioCyci YEAST:YER026C-MONOMER.
    BRENDAi 2.7.8.8. 984.

    Miscellaneous databases

    NextBioi 982893.

    Gene expression databases

    Genevestigatori P08456.

    Family and domain databases

    InterProi IPR004533. CDP-diaglyc--ser_O-PTrfase.
    IPR016271. CDP-diaglyc--ser_O-PTrfase_fun.
    IPR000462. CDP-OH_P_trans.
    [Graphical view ]
    Pfami PF01066. CDP-OH_P_transf. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000852. Phosphatidylserine_synth_fun. 1 hit.
    TIGRFAMsi TIGR00473. pssA. 1 hit.
    PROSITEi PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and characterization of the yeast PSS gene encoding phosphatidylserine synthase."
      Nikawa J., Tsukagoschi Y., Kodaki T., Yamashita S.
      Eur. J. Biochem. 167:7-12(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Primary structure and product characterization of the Saccharomyces cerevisiae CHO1 gene that encodes phosphatidylserine synthase."
      Kiyono K., Miura K., Kushima Y., Hikiji T., Fukushima M., Shibuya I., Ohta A.
      J. Biochem. 102:1089-1100(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-34; SER-42; SER-46; SER-47 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4 AND SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-34; SER-42; SER-46; SER-47 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPSS_YEAST
    AccessioniPrimary (citable) accession number: P08456
    Secondary accession number(s): D3DLS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 143 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3