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Protein

CDP-diacylglycerol--serine O-phosphatidyltransferase

Gene

CHO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine.

Pathway: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--serine O-phosphatidyltransferase (CHO1)
  2. Phosphatidylserine decarboxylase proenzyme 2 (PSD2), Phosphatidylserine decarboxylase proenzyme 1, mitochondrial (PSD1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from CDP-diacylglycerol, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • CDP-diacylglycerol-serine O-phosphatidyltransferase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciYEAST:YER026C-MONOMER.
UniPathwayiUPA00558; UER00615.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-diacylglycerol--serine O-phosphatidyltransferase (EC:2.7.8.8)
Alternative name(s):
Phosphatidylserine synthase
Gene namesi
Name:CHO1
Synonyms:PSS, PSS1
Ordered Locus Names:YER026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER026C.
SGDiS000000828. CHO1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei82 – 10221HelicalSequence AnalysisAdd
BLAST
Transmembranei163 – 18321HelicalSequence AnalysisAdd
BLAST
Transmembranei210 – 23021HelicalSequence AnalysisAdd
BLAST
Transmembranei248 – 26821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: SGD
  • mitochondrial outer membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 276275CDP-diacylglycerol--serine O-phosphatidyltransferasePRO_0000056801Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41Phosphoserine3 Publications
Modified residuei34 – 341Phosphoserine3 Publications
Modified residuei42 – 421Phosphoserine2 Publications
Modified residuei46 – 461Phosphoserine2 Publications
Modified residuei47 – 471Phosphoserine2 Publications
Modified residuei50 – 501Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08456.
PeptideAtlasiP08456.

Interactioni

Protein-protein interaction databases

BioGridi36761. 40 interactions.
DIPiDIP-5331N.
IntActiP08456. 7 interactions.
MINTiMINT-521670.
STRINGi4932.YER026C.

Structurei

3D structure databases

ProteinModelPortaliP08456.
SMRiP08456. Positions 82-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1183.
HOGENOMiHOG000229371.
InParanoidiP08456.
KOiK17103.
OMAiCGMISKS.
OrthoDBiEOG7W15FB.

Family and domain databases

InterProiIPR004533. CDP-diaglyc--ser_O-PTrfase.
IPR016271. CDP-diaglyc--ser_O-PTrfase_fun.
IPR000462. CDP-OH_P_trans.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000852. Phosphatidylserine_synth_fun. 1 hit.
TIGRFAMsiTIGR00473. pssA. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVESDEDFAP QEFPHTDTDV IVNEHRDEND GYASDEVGGT LSRRASSIFS
60 70 80 90 100
INTTPLAPPN ATDIQKFTSD EHHFSMMRNL HMADYITMLN GFSGFYSIVS
110 120 130 140 150
CLRFTLTGKP HYVQRAHFFI LLGMCFDFLD GRVARLRNRS SLMGQELDSL
160 170 180 190 200
ADLVSFGVAP AAIAFAIGFQ TTFDVMILSF FVLCGLARLA RFNVTVAQLP
210 220 230 240 250
KDSSTGKSKY FEGLPMPTTL ALVLGMAYCV RKGLIFDNIP FGIFREDQIL
260 270
EFHPIILVFF IHGCGMISKS LKIPKP
Length:276
Mass (Da):30,805
Last modified:January 23, 2007 - v3
Checksum:iE52B1FC836B67D24
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti123 – 1231G → A in BAA00121 (PubMed:2830250).Curated
Sequence conflicti191 – 1911R → T in BAA00121 (PubMed:2830250).Curated
Sequence conflicti276 – 2761P → A in BAA00121 (PubMed:2830250).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05944 Genomic DNA. Translation: CAA29376.1.
D00171 Genomic DNA. Translation: BAA00121.1.
U18778 Genomic DNA. Translation: AAB64559.1.
BK006939 Genomic DNA. Translation: DAA07679.1.
PIRiS00080.
RefSeqiNP_010943.3. NM_001178917.3.

Genome annotation databases

EnsemblFungiiYER026C; YER026C; YER026C.
GeneIDi856748.
KEGGisce:YER026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05944 Genomic DNA. Translation: CAA29376.1.
D00171 Genomic DNA. Translation: BAA00121.1.
U18778 Genomic DNA. Translation: AAB64559.1.
BK006939 Genomic DNA. Translation: DAA07679.1.
PIRiS00080.
RefSeqiNP_010943.3. NM_001178917.3.

3D structure databases

ProteinModelPortaliP08456.
SMRiP08456. Positions 82-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36761. 40 interactions.
DIPiDIP-5331N.
IntActiP08456. 7 interactions.
MINTiMINT-521670.
STRINGi4932.YER026C.

Proteomic databases

PaxDbiP08456.
PeptideAtlasiP08456.

Protocols and materials databases

DNASUi856748.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER026C; YER026C; YER026C.
GeneIDi856748.
KEGGisce:YER026C.

Organism-specific databases

EuPathDBiFungiDB:YER026C.
SGDiS000000828. CHO1.

Phylogenomic databases

eggNOGiCOG1183.
HOGENOMiHOG000229371.
InParanoidiP08456.
KOiK17103.
OMAiCGMISKS.
OrthoDBiEOG7W15FB.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00615.
BioCyciYEAST:YER026C-MONOMER.

Miscellaneous databases

NextBioi982893.
PROiP08456.

Family and domain databases

InterProiIPR004533. CDP-diaglyc--ser_O-PTrfase.
IPR016271. CDP-diaglyc--ser_O-PTrfase_fun.
IPR000462. CDP-OH_P_trans.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000852. Phosphatidylserine_synth_fun. 1 hit.
TIGRFAMsiTIGR00473. pssA. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and characterization of the yeast PSS gene encoding phosphatidylserine synthase."
    Nikawa J., Tsukagoschi Y., Kodaki T., Yamashita S.
    Eur. J. Biochem. 167:7-12(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Primary structure and product characterization of the Saccharomyces cerevisiae CHO1 gene that encodes phosphatidylserine synthase."
    Kiyono K., Miura K., Kushima Y., Hikiji T., Fukushima M., Shibuya I., Ohta A.
    J. Biochem. 102:1089-1100(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-34; SER-42; SER-46; SER-47 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4 AND SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4; SER-34; SER-42; SER-46; SER-47 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSS_YEAST
AccessioniPrimary (citable) accession number: P08456
Secondary accession number(s): D3DLS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.