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P08455 (MTP2_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase NgoPII

Short name=M.NgoPII
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase NgoPII
Gene names
Name:ngoPIIM
Synonyms:dcrM
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the NgoPII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence caution

The sequence AAA17019.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA30038.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA36888.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330Modification methylase NgoPII
PRO_0000087900

Regions

Domain1 – 330330SAM-dependent MTase C5-type

Sites

Active site731 By similarity

Sequences

Sequence LengthMass (Da)Tools
P08455 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 0A384A98B1EB03E1

FASTA33037,222
        10         20         30         40         50         60 
MKIISLFSGC GGLDLGFEKA GFEIPAANEY DKTIWATFKA NHPKTHLIEG DIRKIKEEDF 

        70         80         90        100        110        120 
PEEIDGIIGG PPCQSWSEAG ALRGIDDARG QLFFDYIRIL KSKQPKFFLA ENVSGMLANR 

       130        140        150        160        170        180 
HNGAVQNLLK MFDGCGYDVT LTMANAKDYG VAQERKRVFY IGFRKDLEIK FSFPKGSTVE 

       190        200        210        220        230        240 
DKDKITLKDV IWDLQDTAVP SAPQNKTNPD AVNNNEYFTG SFSPIFMSRN RVKAWDEQGF 

       250        260        270        280        290        300 
TVQASGRQCQ LHPQAPKMEK HGANDYRFAA GKETLYRRMT VREVARIQGF PDNFKFIYQN 

       310        320        330 
VNDAYKMIGN AVPVNLAYEI AAAIKKTLER 

« Hide

References

[1]"Sequence analysis of the NgoPII methyltransferase gene from Neisseria gonorrhoeae P9: homologies with other enzymes recognizing the sequence 5'-GGCC-3'."
Sullivan K.M., Saunders J.R.
Nucleic Acids Res. 16:4369-4387(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: P9.
[2]"Nucleotide sequence and genetic organization of the NgoPII restriction-modification system of Neisseria gonorrhoeae."
Sullivan K.M., Saunders J.R.
Mol. Gen. Genet. 216:380-387(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: P9.
[3]"Natural variation of the NgoII restriction-modification system of Neisseria gonorrhoeae."
Gunn J.S., Stein D.C.
Gene 132:15-20(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 33084 / F62 / M-1914.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06965 Genomic DNA. Translation: CAA30038.1. Different initiation.
X52661 Genomic DNA. Translation: CAA36888.1. Different initiation.
L14564 Unassigned DNA. Translation: AAA17019.1. Different initiation.
PIRCTNHP2. S00920.

3D structure databases

ProteinModelPortalP08455.
SMRP08455. Positions 1-328.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3609. M.NgoLII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTP2_NEIGO
AccessionPrimary (citable) accession number: P08455
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries