P08455 (MTP2_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase NgoPII
Short name=M.NgoPII
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase NgoPII

Gene names

Name:	ngoPIIM
Synonyms:	dcrM

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the NgoPII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.
Sequence caution	The sequence AAA17019.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA30038.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA36888.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Modification methylase NgoPII		PRO_0000087900
Sites
	Active site	73	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08455 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 0A384A98B1EB03E1	FASTA	330	37,222
	10 20 30 40 50 60 MKIISLFSGC GGLDLGFEKA GFEIPAANEY DKTIWATFKA NHPKTHLIEG DIRKIKEEDF 70 80 90 100 110 120 PEEIDGIIGG PPCQSWSEAG ALRGIDDARG QLFFDYIRIL KSKQPKFFLA ENVSGMLANR 130 140 150 160 170 180 HNGAVQNLLK MFDGCGYDVT LTMANAKDYG VAQERKRVFY IGFRKDLEIK FSFPKGSTVE 190 200 210 220 230 240 DKDKITLKDV IWDLQDTAVP SAPQNKTNPD AVNNNEYFTG SFSPIFMSRN RVKAWDEQGF 250 260 270 280 290 300 TVQASGRQCQ LHPQAPKMEK HGANDYRFAA GKETLYRRMT VREVARIQGF PDNFKFIYQN 310 320 330 VNDAYKMIGN AVPVNLAYEI AAAIKKTLER

References

[1]	"Sequence analysis of the NgoPII methyltransferase gene from Neisseria gonorrhoeae P9: homologies with other enzymes recognizing the sequence 5'-GGCC-3'." Sullivan K.M., Saunders J.R. Nucleic Acids Res. 16:4369-4387(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: P9.
[2]	"Nucleotide sequence and genetic organization of the NgoPII restriction-modification system of Neisseria gonorrhoeae." Sullivan K.M., Saunders J.R. Mol. Gen. Genet. 216:380-387(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: P9.
[3]	"Natural variation of the NgoII restriction-modification system of Neisseria gonorrhoeae." Gunn J.S., Stein D.C. Gene 132:15-20(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33084 / F62 / M-1914.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06965 Genomic DNA. Translation: CAA30038.1. Different initiation. X52661 Genomic DNA. Translation: CAA36888.1. Different initiation. L14564 Unassigned DNA. Translation: AAA17019.1. Different initiation.
PIR	CTNHP2. S00920.
3D structure databases
ProteinModelPortal	P08455.
SMR	P08455. Positions 1-328.
ModBase	Search...
Protein family/group databases
REBASE	3609. M.NgoLII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTP2_NEIGO

Accession

Primary (citable) accession number: P08455

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program