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P08455

- MTP2_NEIGO

UniProt

P08455 - MTP2_NEIGO

Protein

Modification methylase NgoPII

Gene

ngoPIIM

Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the NgoPII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3609. M.NgoLII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase NgoPII (EC:2.1.1.37)
    Short name:
    M.NgoPII
    Alternative name(s):
    Cytosine-specific methyltransferase NgoPII
    Gene namesi
    Name:ngoPIIM
    Synonyms:dcrM
    OrganismiNeisseria gonorrhoeae
    Taxonomic identifieri485 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Modification methylase NgoPIIPRO_0000087900Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP08455.
    SMRiP08455. Positions 1-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 330330SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08455-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIISLFSGC GGLDLGFEKA GFEIPAANEY DKTIWATFKA NHPKTHLIEG    50
    DIRKIKEEDF PEEIDGIIGG PPCQSWSEAG ALRGIDDARG QLFFDYIRIL 100
    KSKQPKFFLA ENVSGMLANR HNGAVQNLLK MFDGCGYDVT LTMANAKDYG 150
    VAQERKRVFY IGFRKDLEIK FSFPKGSTVE DKDKITLKDV IWDLQDTAVP 200
    SAPQNKTNPD AVNNNEYFTG SFSPIFMSRN RVKAWDEQGF TVQASGRQCQ 250
    LHPQAPKMEK HGANDYRFAA GKETLYRRMT VREVARIQGF PDNFKFIYQN 300
    VNDAYKMIGN AVPVNLAYEI AAAIKKTLER 330
    Length:330
    Mass (Da):37,222
    Last modified:February 1, 1994 - v2
    Checksum:i0A384A98B1EB03E1
    GO

    Sequence cautioni

    The sequence AAA17019.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA30038.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA36888.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06965 Genomic DNA. Translation: CAA30038.1. Different initiation.
    X52661 Genomic DNA. Translation: CAA36888.1. Different initiation.
    L14564 Unassigned DNA. Translation: AAA17019.1. Different initiation.
    PIRiS00920. CTNHP2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06965 Genomic DNA. Translation: CAA30038.1 . Different initiation.
    X52661 Genomic DNA. Translation: CAA36888.1 . Different initiation.
    L14564 Unassigned DNA. Translation: AAA17019.1 . Different initiation.
    PIRi S00920. CTNHP2.

    3D structure databases

    ProteinModelPortali P08455.
    SMRi P08455. Positions 1-328.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3609. M.NgoLII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the NgoPII methyltransferase gene from Neisseria gonorrhoeae P9: homologies with other enzymes recognizing the sequence 5'-GGCC-3'."
      Sullivan K.M., Saunders J.R.
      Nucleic Acids Res. 16:4369-4387(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: P9.
    2. "Nucleotide sequence and genetic organization of the NgoPII restriction-modification system of Neisseria gonorrhoeae."
      Sullivan K.M., Saunders J.R.
      Mol. Gen. Genet. 216:380-387(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: P9.
    3. "Natural variation of the NgoII restriction-modification system of Neisseria gonorrhoeae."
      Gunn J.S., Stein D.C.
      Gene 132:15-20(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 33084 / F62 / M-1914.

    Entry informationi

    Entry nameiMTP2_NEIGO
    AccessioniPrimary (citable) accession number: P08455
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3