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Protein

Modification methylase NgoPII

Gene

ngoPIIM

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the NgoPII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3609. M.NgoLII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase NgoPII (EC:2.1.1.37)
Short name:
M.NgoPII
Alternative name(s):
Cytosine-specific methyltransferase NgoPII
Gene namesi
Name:ngoPIIM
Synonyms:dcrM
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879001 – 330Modification methylase NgoPIIAdd BLAST330

Interactioni

Protein-protein interaction databases

STRINGi528357.NgonPI_010100010200.

Structurei

3D structure databases

ProteinModelPortaliP08455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 330SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST330

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DVF. Bacteria.
COG0270. LUCA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08455-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIISLFSGC GGLDLGFEKA GFEIPAANEY DKTIWATFKA NHPKTHLIEG
60 70 80 90 100
DIRKIKEEDF PEEIDGIIGG PPCQSWSEAG ALRGIDDARG QLFFDYIRIL
110 120 130 140 150
KSKQPKFFLA ENVSGMLANR HNGAVQNLLK MFDGCGYDVT LTMANAKDYG
160 170 180 190 200
VAQERKRVFY IGFRKDLEIK FSFPKGSTVE DKDKITLKDV IWDLQDTAVP
210 220 230 240 250
SAPQNKTNPD AVNNNEYFTG SFSPIFMSRN RVKAWDEQGF TVQASGRQCQ
260 270 280 290 300
LHPQAPKMEK HGANDYRFAA GKETLYRRMT VREVARIQGF PDNFKFIYQN
310 320 330
VNDAYKMIGN AVPVNLAYEI AAAIKKTLER
Length:330
Mass (Da):37,222
Last modified:February 1, 1994 - v2
Checksum:i0A384A98B1EB03E1
GO

Sequence cautioni

The sequence AAA17019 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA30038 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA36888 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06965 Genomic DNA. Translation: CAA30038.1. Different initiation.
X52661 Genomic DNA. Translation: CAA36888.1. Different initiation.
L14564 Unassigned DNA. Translation: AAA17019.1. Different initiation.
PIRiS00920. CTNHP2.
RefSeqiWP_003690024.1. NZ_LOIB01000055.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06965 Genomic DNA. Translation: CAA30038.1. Different initiation.
X52661 Genomic DNA. Translation: CAA36888.1. Different initiation.
L14564 Unassigned DNA. Translation: AAA17019.1. Different initiation.
PIRiS00920. CTNHP2.
RefSeqiWP_003690024.1. NZ_LOIB01000055.1.

3D structure databases

ProteinModelPortaliP08455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi528357.NgonPI_010100010200.

Protein family/group databases

REBASEi3609. M.NgoLII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105DVF. Bacteria.
COG0270. LUCA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR031303. C5_meth_CS.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTP2_NEIGO
AccessioniPrimary (citable) accession number: P08455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1994
Last modified: October 5, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.