P08440 (ALF_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Fructose-bisphosphate aldolase, cytoplasmic isozyme EC=4.1.2.13
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to karrikin Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to salt stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene cytosol Inferred from electronic annotation. Source: EnsemblPlants/Gramene mitochondrial envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleolus Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	copper ion binding Inferred from electronic annotation. Source: EnsemblPlants/Gramene fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Fructose-bisphosphate aldolase, cytoplasmic isozyme		PRO_0000216921
Sites
	Active site	183	1	Proton acceptor By similarity
	Active site	225	1	Schiff-base intermediate with dihydroxyacetone-P
	Binding site	52	1	Substrate
	Binding site	142	1	Substrate
	Site	355	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

Sequences

	Sequence		Length	Mass (Da)
	P08440 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 54B480978ECD1470	FASTA	355	38,604
	10 20 30 40 50 60 MSAYCGKYKD ELIKNAAYIG TPGKGILAAD ESTGTIGKRL SSINVENVEE NRRALRELLF 70 80 90 100 110 120 CCPGALQYIS GVILFEETLY QKTKDGKPFV DVLKEGGVLP GIKVDKGTIE VVGTDKETTT 130 140 150 160 170 180 QGHDDLGKRC AKYYEAGARF AKWRAVLKIG PNEPSQLAID LNAQGLARYA IICQENGLVP 190 200 210 220 230 240 IVEPEILVDG PHDIDRCAYV TETVLAACYK ALNEHHVLLE GTLLKPNMVT PGSDSKKVTP 250 260 270 280 290 300 EVIAEYTVRT LQRTVPAAVP AVLFLSGGQS EEEATRNLNA MNKLSTKKPW SLSFSFGRAL 310 320 330 340 350 QASTLKAWAG KVENLEKARA AFLARCKANS EATLGTYKGD AAADTESLHV KDYKY

References

[1]	"Anaerobically regulated aldolase gene of maize. A chimaeric origin?" Dennis E.S., Gerlach W.L., Walker J.C., Lavin M., Peacock W.J. J. Mol. Biol. 202:759-767(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The complete amino acid sequence for the anaerobically induced aldolase from maize derived from cDNA clones." Kelley P.M., Tolan D.R. Plant Physiol. 82:1076-1080(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12872 Genomic DNA. Translation: CAA31366.1. M16220 mRNA. Translation: AAA33435.1.
PIR	ADZM. S07789.
RefSeq	NP_001105336.1. NM_001111866.1.
UniGene	Zm.72659.
3D structure databases
ProteinModelPortal	P08440.
SMR	P08440. Positions 9-337.
ModBase	Search...
Proteomic databases
PRIDE	P08440.
ProMEX	P08440.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542261.
KEGG	zma:542261.
Organism-specific databases
Gramene	P08440.
MaizeGDB	24903.
Phylogenomic databases
HOGENOM	HOG000220876.
KO	K01623.
Enzyme and pathway databases
SABIO-RK	P08440.
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
PANTHER	PTHR11627. PTHR11627. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF_MAIZE

Accession

Primary (citable) accession number: P08440

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program