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Protein

Ornithine decarboxylase

Gene

SPE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.1 Publication

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-ornithine = putrescine + CO2.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Enzyme regulationi

Inhibited by antizyme (AZ) OAZ1 in response to polyamine levels. AZ inhibits the assembly of the functional homodimer by binding to ODC monomers and targeting them for ubiquitin-independent proteolytic destruction by the 26S proteasome.1 Publication

Pathwayi: putrescine biosynthesis via L-ornithine pathway

This protein is involved in step 1 of the subpathway that synthesizes putrescine from L-ornithine.1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Ornithine decarboxylase (SPE1)
This subpathway is part of the pathway putrescine biosynthesis via L-ornithine pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes putrescine from L-ornithine, the pathway putrescine biosynthesis via L-ornithine pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei244Stacks against the aromatic ring of pyridoxal phosphate and stabilizes reaction intermediatesBy similarity1
Binding sitei247Pyridoxal phosphateBy similarity1
Binding sitei286Pyridoxal phosphate; via amino nitrogenBy similarity1
Active sitei411Proton donor; shared with dimeric partnerBy similarity1
Binding sitei412Substrate; shared with dimeric partnerBy similarity1
Binding sitei441Pyridoxal phosphateBy similarity1

GO - Molecular functioni

  • ornithine decarboxylase activity Source: SGD

GO - Biological processi

  • pantothenate biosynthetic process Source: SGD
  • putrescine biosynthetic process Source: SGD
  • putrescine biosynthetic process from ornithine Source: GO_Central

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processPolyamine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YKL184W-MONOMER
YEAST:YKL184W-MONOMER
ReactomeiR-SCE-351143 Agmatine biosynthesis
R-SCE-351202 Metabolism of polyamines
UniPathwayiUPA00535; UER00288

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine decarboxylase (EC:4.1.1.171 Publication)
Short name:
ODC
Gene namesi
Name:SPE11 Publication
Synonyms:ORD1
Ordered Locus Names:YKL184WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL184W
SGDiS000001667 SPE1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001499091 – 466Ornithine decarboxylaseAdd BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP08432
PRIDEiP08432

Interactioni

Subunit structurei

Homodimer (PubMed:2669750). Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi33938502 interactors.
IntActiP08432 6 interactors.
STRINGi4932.YKL184W

Structurei

3D structure databases

ProteinModelPortaliP08432
SMRiP08432
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni318 – 321Pyridoxal phosphate bindingBy similarity4
Regioni362 – 363Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000011560
HOGENOMiHOG000274133
InParanoidiP08432
KOiK01581
OMAiNDKFSSG
OrthoDBiEOG092C2Q2K

Family and domain databases

Gene3Di2.40.37.103 hits
3.20.20.101 hit
InterProiView protein in InterPro
IPR009006 Ala_racemase/Decarboxylase_C
IPR022643 De-COase2_C
IPR022657 De-COase2_CS
IPR022644 De-COase2_N
IPR022653 De-COase2_pyr-phos_BS
IPR000183 Orn/DAP/Arg_de-COase
IPR002433 Orn_de-COase
IPR029066 PLP-binding_barrel
PfamiView protein in Pfam
PF02784 Orn_Arg_deC_N, 1 hit
PF00278 Orn_DAP_Arg_deC, 1 hit
PRINTSiPR01179 ODADCRBXLASE
PR01182 ORNDCRBXLASE
SUPFAMiSSF50621 SSF50621, 1 hit
SSF51419 SSF51419, 1 hit
PROSITEiView protein in PROSITE
PS00878 ODR_DC_2_1, 1 hit
PS00879 ODR_DC_2_2, 1 hit

Sequencei

Sequence statusi: Complete.

P08432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTQVGNAL SSSTTTLVDL SNSTVTQKKQ YYKDGETLHN LLLELKNNQD
60 70 80 90 100
LELLPHEQAH PKIFQALKAR IGRINNETCD PGEENSFFIC DLGEVKRLFN
110 120 130 140 150
NWVKELPRIK PFYAVKCNPD TKVLSLLAEL GVNFDCASKV EIDRVLSMNI
160 170 180 190 200
SPDRIVYANP CKVASFIRYA ASKNVMKSTF DNVEELHKIK KFHPESQLLL
210 220 230 240 250
RIATDDSTAQ CRLSTKYGCE MENVDVLLKA IKELGLNLAG VSFHVGSGAS
260 270 280 290 300
DFTSLYKAVR DARTVFDKAA NEYGLPPLKI LDVGGGFQFE SFKESTAVLR
310 320 330 340 350
LALEEFFPVG CGVDIIAEPG RYFVATAFTL ASHVIAKRKL SENEAMIYTN
360 370 380 390 400
DGVYGNMNCI LFDHQEPHPR TLYHNLEFHY DDFESTTAVL DSINKTRSEY
410 420 430 440 450
PYKVSIWGPT CDGLDCIAKE YYMKHDVIVG DWFYFPALGA YTSSAATQFN
460
GFEQTADIVY IDSELD
Length:466
Mass (Da):52,285
Last modified:August 1, 1988 - v1
Checksum:i2EBD7EE8CFAA67CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02777 Genomic DNA Translation: AAA34829.1
X74151 Genomic DNA Translation: CAA52254.1
Z28184 Genomic DNA Translation: CAA82027.1
BK006944 Genomic DNA Translation: DAA08982.1
PIRiA28437 DCBYO
RefSeqiNP_012737.1, NM_001179750.1

Genome annotation databases

EnsemblFungiiYKL184W; YKL184W; YKL184W
GeneIDi853651
KEGGisce:YKL184W

Similar proteinsi

Entry informationi

Entry nameiDCOR_YEAST
AccessioniPrimary (citable) accession number: P08432
Secondary accession number(s): D6VX16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: March 28, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome