P08431 (GAL7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactose-1-phosphate uridylyltransferase
Short name=Gal-1-P uridylyltransferase
EC=2.7.7.12

Alternative name(s):
UDP-glucose--hexose-1-phosphate uridylyltransferase

Gene names

Name:	GAL7
Ordered Locus Names:	YBR018C
ORF Names:	YBR0226

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	UDP-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-galactose.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Pathway	Carbohydrate metabolism; galactose metabolism.
Sequence similarities	Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Ligand	Iron Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	galactose catabolic process via UDP-galactose Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	UDP-glucose:hexose-1-phosphate uridylyltransferase activity Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 366	365	Galactose-1-phosphate uridylyltransferase		PRO_0000169893
Sites
	Active site	182	1	Tele-UMP-histidine intermediate By similarity
	Metal binding	54	1	Zinc By similarity
	Metal binding	57	1	Zinc By similarity
	Metal binding	124	1	Zinc By similarity
	Metal binding	180	1	Zinc By similarity
	Metal binding	198	1	Iron By similarity
	Metal binding	297	1	Iron By similarity
	Metal binding	314	1	Iron By similarity
	Metal binding	316	1	Iron By similarity
Experimental info
	Sequence conflict	11	1	H → Y in AAA34628. Ref.5
	Sequence conflict	58	1	P → H in AAA34628. Ref.5
	Sequence conflict	85 – 86	2	RL → S in AAA34627. Ref.1
	Sequence conflict	267	1	V → A in AAA34627. Ref.1
	Sequence conflict	345	1	T → I in AAA34627. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P08431 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 960F75532D2621DC	FASTA	366	42,385
	10 20 30 40 50 60 MTAEEFDFSS HSHRRYNPLT DSWILVSPHR AKRPWLGQQE AAYKPTAPLY DPKCYLCPGN 70 80 90 100 110 120 KRATGNLNPR YESTYIFPND YAAVRLDQPI LPQNDSNEDN LKNRLLKVQS VRGNCFVICF 130 140 150 160 170 180 SPNHNLTIPQ MKQSDLVHIV NSWQALTDDL SREARENHKP FKYVQIFENK GTAMGCSNLH 190 200 210 220 230 240 PHGQAWCLES IPSEVSQELK SFDKYKREHN TDLFADYVKL ESREKSRVVV ENESFIVVVP 250 260 270 280 290 300 YWAIWPFETL VISKKKLASI SQFNQMVKED LASILKQLTI KYDNLFETSF PYSMGIHQAP 310 320 330 340 350 360 LNATGDELSN SWFHMHFYPP LLRSATVRKF LVGFELLGEP QRDLTSEQAA EKLRNLDGQI HYLQRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the Saccharomyces cerevisiae GAL7 gene." Tajima M., Nogi Y., Fukasawa T. Yeast 1:67-77(1985) [PubMed: 2851900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and functional analysis of a 7.2 kb fragment of Saccharomyces cerevisiae chromosome II including GAL7 and GAL10 and a new essential open reading frame." Schaaff-Gerstenschlaeger I., Schindwolf T., Lehnert W., Rose M., Zimmermann F.K. Yeast 11:79-83(1995) [PubMed: 7762304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Sequence of the Saccharomyces GAL region and its transcription in vivo." Citron B.A., Donelson J.E. J. Bacteriol. 158:269-278(1984) [PubMed: 6715281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-185. Strain: Carlsbergensis.
[6]	"Nucleotide sequence of the transcriptional initiation region of the yeast GAL7 gene." Nogi Y., Fukasawa T. Nucleic Acids Res. 11:8555-8568(1983) [PubMed: 6324089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21, PROTEIN SEQUENCE OF 2-8.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81324 Genomic DNA. Translation: CAA57105.1. M12348 Genomic DNA. Translation: AAA34627.1. X00215 Genomic DNA. Translation: CAA25039.1. Z35887 Genomic DNA. Translation: CAA84960.1. K01752 Genomic DNA. Translation: AAA34628.1. BK006936 Genomic DNA. Translation: DAA07140.1.
PIR	XNBYUG. S45873.
RefSeq	NP_009574.1. NM_001178366.1.
3D structure databases
ProteinModelPortal	P08431.
SMR	P08431. Positions 5-365.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5357N.
IntAct	P08431. 9 interactions.
MINT	MINT-504266.
STRING	P08431.
Proteomic databases
PeptideAtlas	P08431.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR018C; YBR018C; YBR018C.
GeneID	852306.
KEGG	sce:YBR018C.
NMPDR	fig|4932.3.peg.269.
Organism-specific databases
SGD	S000000222. GAL7.
Phylogenomic databases
eggNOG	fuNOG06817.
GeneTree	EFGT00050000004427.
HOGENOM	HBG600409.
OMA	GIHQAPL.
OrthoDB	EOG45QMP2.
Gene expression databases
ArrayExpress	P08431.
Genevestigator	P08431.
GermOnline	YBR018C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001937. GalP_UDPtransf1. IPR019779. GalP_UDPtransf1_His-AS. IPR005850. GalP_Utransf_C. IPR005849. GalP_Utransf_N. IPR011146. His_triad-like_motif. IPR011151. His_triad_motif. [Graphical view]
Gene3D	G3DSA:3.30.428.10. His_triad_motif. 2 hits.
KO	K00965.
PANTHER	PTHR11943. GalP_UDPtransf1. 1 hit.
Pfam	PF02744. GalP_UDP_tr_C. 1 hit. PF01087. GalP_UDP_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000808. GalT. 1 hit.
SUPFAM	SSF54197. His_triad-like_motif. 2 hits.
TIGRFAMs	TIGR00209. GalT_1. 1 hit.
PROSITE	PS00117. GAL_P_UDP_TRANSF_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970978.

Entry information

Entry name

GAL7_YEAST

Accession

Primary (citable) accession number: P08431
Secondary accession number(s): D6VQ20, P04398

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 117 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program