Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactose-1-phosphate uridylyltransferase

Gene

GAL7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541ZincPROSITE-ProRule annotation
Metal bindingi57 – 571ZincPROSITE-ProRule annotation
Metal bindingi124 – 1241ZincPROSITE-ProRule annotation
Metal bindingi180 – 1801ZincPROSITE-ProRule annotation
Active sitei182 – 1821Tele-UMP-histidine intermediatePROSITE-ProRule annotation
Metal bindingi198 – 1981IronBy similarity
Metal bindingi297 – 2971IronBy similarity
Metal bindingi314 – 3141IronBy similarity
Metal bindingi316 – 3161IronBy similarity

GO - Molecular functioni

GO - Biological processi

  • galactose catabolic process via UDP-galactose Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YBR018C-MONOMER.
ReactomeiR-SCE-70370. Galactose catabolism.
UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose-1-phosphate uridylyltransferase (EC:2.7.7.12)
Short name:
Gal-1-P uridylyltransferase
Alternative name(s):
UDP-glucose--hexose-1-phosphate uridylyltransferase
Gene namesi
Name:GAL7
Ordered Locus Names:YBR018C
ORF Names:YBR0226
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR018C.
SGDiS000000222. GAL7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 366365Galactose-1-phosphate uridylyltransferasePRO_0000169893Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP08431.

PTM databases

iPTMnetiP08431.

Interactioni

Protein-protein interaction databases

BioGridi32721. 43 interactions.
DIPiDIP-5357N.
IntActiP08431. 4 interactions.
MINTiMINT-504266.

Structurei

3D structure databases

ProteinModelPortaliP08431.
SMRiP08431. Positions 5-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016188.
HOGENOMiHOG000230490.
InParanoidiP08431.
KOiK00965.
OMAiGQWVLVC.
OrthoDBiEOG70ZZXT.

Family and domain databases

Gene3Di3.30.428.10. 2 hits.
InterProiIPR001937. GalP_UDPtransf1.
IPR019779. GalP_UDPtransf1_His-AS.
IPR005850. GalP_Utransf_C.
IPR005849. GalP_Utransf_N.
IPR011146. HIT-like.
[Graphical view]
PANTHERiPTHR11943. PTHR11943. 1 hit.
PfamiPF02744. GalP_UDP_tr_C. 1 hit.
PF01087. GalP_UDP_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000808. GalT. 1 hit.
SUPFAMiSSF54197. SSF54197. 2 hits.
TIGRFAMsiTIGR00209. galT_1. 1 hit.
PROSITEiPS00117. GAL_P_UDP_TRANSF_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAEEFDFSS HSHRRYNPLT DSWILVSPHR AKRPWLGQQE AAYKPTAPLY
60 70 80 90 100
DPKCYLCPGN KRATGNLNPR YESTYIFPND YAAVRLDQPI LPQNDSNEDN
110 120 130 140 150
LKNRLLKVQS VRGNCFVICF SPNHNLTIPQ MKQSDLVHIV NSWQALTDDL
160 170 180 190 200
SREARENHKP FKYVQIFENK GTAMGCSNLH PHGQAWCLES IPSEVSQELK
210 220 230 240 250
SFDKYKREHN TDLFADYVKL ESREKSRVVV ENESFIVVVP YWAIWPFETL
260 270 280 290 300
VISKKKLASI SQFNQMVKED LASILKQLTI KYDNLFETSF PYSMGIHQAP
310 320 330 340 350
LNATGDELSN SWFHMHFYPP LLRSATVRKF LVGFELLGEP QRDLTSEQAA
360
EKLRNLDGQI HYLQRL
Length:366
Mass (Da):42,385
Last modified:January 23, 2007 - v4
Checksum:i960F75532D2621DC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111H → Y in AAA34628 (PubMed:6715281).Curated
Sequence conflicti58 – 581P → H in AAA34628 (PubMed:6715281).Curated
Sequence conflicti85 – 862RL → S in AAA34627 (PubMed:2851900).Curated
Sequence conflicti267 – 2671V → A in AAA34627 (PubMed:2851900).Curated
Sequence conflicti345 – 3451T → I in AAA34627 (PubMed:2851900).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81324 Genomic DNA. Translation: CAA57105.1.
M12348 Genomic DNA. Translation: AAA34627.1.
X00215 Genomic DNA. Translation: CAA25039.1.
Z35887 Genomic DNA. Translation: CAA84960.1.
K01752 Genomic DNA. Translation: AAA34628.1.
BK006936 Genomic DNA. Translation: DAA07140.1.
PIRiS45873. XNBYUG.
RefSeqiNP_009574.1. NM_001178366.1.

Genome annotation databases

EnsemblFungiiYBR018C; YBR018C; YBR018C.
GeneIDi852306.
KEGGisce:YBR018C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81324 Genomic DNA. Translation: CAA57105.1.
M12348 Genomic DNA. Translation: AAA34627.1.
X00215 Genomic DNA. Translation: CAA25039.1.
Z35887 Genomic DNA. Translation: CAA84960.1.
K01752 Genomic DNA. Translation: AAA34628.1.
BK006936 Genomic DNA. Translation: DAA07140.1.
PIRiS45873. XNBYUG.
RefSeqiNP_009574.1. NM_001178366.1.

3D structure databases

ProteinModelPortaliP08431.
SMRiP08431. Positions 5-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32721. 43 interactions.
DIPiDIP-5357N.
IntActiP08431. 4 interactions.
MINTiMINT-504266.

PTM databases

iPTMnetiP08431.

Proteomic databases

MaxQBiP08431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR018C; YBR018C; YBR018C.
GeneIDi852306.
KEGGisce:YBR018C.

Organism-specific databases

EuPathDBiFungiDB:YBR018C.
SGDiS000000222. GAL7.

Phylogenomic databases

GeneTreeiENSGT00390000016188.
HOGENOMiHOG000230490.
InParanoidiP08431.
KOiK00965.
OMAiGQWVLVC.
OrthoDBiEOG70ZZXT.

Enzyme and pathway databases

UniPathwayiUPA00214.
BioCyciYEAST:YBR018C-MONOMER.
ReactomeiR-SCE-70370. Galactose catabolism.

Miscellaneous databases

PROiP08431.

Family and domain databases

Gene3Di3.30.428.10. 2 hits.
InterProiIPR001937. GalP_UDPtransf1.
IPR019779. GalP_UDPtransf1_His-AS.
IPR005850. GalP_Utransf_C.
IPR005849. GalP_Utransf_N.
IPR011146. HIT-like.
[Graphical view]
PANTHERiPTHR11943. PTHR11943. 1 hit.
PfamiPF02744. GalP_UDP_tr_C. 1 hit.
PF01087. GalP_UDP_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000808. GalT. 1 hit.
SUPFAMiSSF54197. SSF54197. 2 hits.
TIGRFAMsiTIGR00209. galT_1. 1 hit.
PROSITEiPS00117. GAL_P_UDP_TRANSF_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of the Saccharomyces cerevisiae GAL7 gene."
    Tajima M., Nogi Y., Fukasawa T.
    Yeast 1:67-77(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence and functional analysis of a 7.2 kb fragment of Saccharomyces cerevisiae chromosome II including GAL7 and GAL10 and a new essential open reading frame."
    Schaaff-Gerstenschlaeger I., Schindwolf T., Lehnert W., Rose M., Zimmermann F.K.
    Yeast 11:79-83(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Sequence of the Saccharomyces GAL region and its transcription in vivo."
    Citron B.A., Donelson J.E.
    J. Bacteriol. 158:269-278(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-185.
    Strain: Carlsbergensis.
  6. "Nucleotide sequence of the transcriptional initiation region of the yeast GAL7 gene."
    Nogi Y., Fukasawa T.
    Nucleic Acids Res. 11:8555-8568(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21, PROTEIN SEQUENCE OF 2-8.
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGAL7_YEAST
AccessioniPrimary (citable) accession number: P08431
Secondary accession number(s): D6VQ20, P04398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 154 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.