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P08427

- SFTPA_RAT

UniProt

P08427 - SFTPA_RAT

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Protein

Pulmonary surfactant-associated protein A

Gene

Sftpa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei21 – 211Not glycosylated
Metal bindingi215 – 2151Calcium
Metal bindingi217 – 2171Calcium; via carbonyl oxygen
Metal bindingi234 – 2341Calcium
Metal bindingi235 – 2351Calcium

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to mechanical stimulus Source: RGD
  2. cellular response to nitric oxide Source: RGD
  3. circadian rhythm Source: RGD
  4. positive regulation of phagocytosis Source: RGD
  5. respiratory gaseous exchange Source: UniProtKB-KW
  6. response to epidermal growth factor Source: RGD
  7. response to glucocorticoid Source: RGD
  8. response to hormone Source: RGD
  9. response to hyperoxia Source: RGD
  10. response to hypoxia Source: RGD
  11. response to interleukin-6 Source: RGD
  12. response to lipopolysaccharide Source: RGD
  13. response to retinoic acid Source: RGD
  14. response to vitamin A Source: RGD
Complete GO annotation...

Keywords - Biological processi

Gaseous exchange

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_239501. Signal regulatory protein (SIRP) family interactions.
REACT_247122. Toll Like Receptor 4 (TLR4) Cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein A
Short name:
PSAP
Short name:
PSP-A
Short name:
SP-A
Gene namesi
Name:Sftpa1
Synonyms:Sftp-1, Sftp1, Sftpa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 16

Organism-specific databases

RGDi3665. Sftpa1.

Subcellular locationi

GO - Cellular componenti

  1. collagen trimer Source: UniProtKB-KW
  2. cytoplasmic vesicle Source: RGD
  3. extracellular space Source: RGD
  4. multivesicular body Source: RGD
  5. proteinaceous extracellular matrix Source: UniProtKB-KW
  6. rough endoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 248228Pulmonary surfactant-associated protein APRO_0000017462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 – 26InterchainPROSITE-ProRule annotation
Modified residuei30 – 3014-hydroxyproline1 Publication
Modified residuei33 – 3314-hydroxyproline1 Publication
Modified residuei36 – 3614-hydroxyproline1 Publication
Modified residuei42 – 4214-hydroxyproline1 Publication
Modified residuei54 – 5414-hydroxyproline1 Publication
Modified residuei57 – 5714-hydroxyproline1 Publication
Modified residuei63 – 6314-hydroxyproline1 Publication
Modified residuei67 – 6714-hydroxyproline1 Publication
Modified residuei70 – 7014-hydroxyproline1 Publication
Modified residuei76 – 7614-hydroxyproline1 Publication
Disulfide bondi155 ↔ 2461 PublicationPROSITE-ProRule annotation
Glycosylationi207 – 2071N-linked (GlcNAc...)Curated
Disulfide bondi224 ↔ 2381 PublicationPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

PaxDbiP08427.
PRIDEiP08427.

Expressioni

Gene expression databases

GenevestigatoriP08427.

Interactioni

Subunit structurei

Oligomeric complex of 6 set of homotrimers.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039850.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi105 – 12723Combined sources
Beta strandi132 – 1343Combined sources
Beta strandi137 – 14610Combined sources
Helixi148 – 15710Combined sources
Beta strandi160 – 1623Combined sources
Helixi168 – 18114Combined sources
Beta strandi185 – 1917Combined sources
Beta strandi198 – 2003Combined sources
Beta strandi201 – 2033Combined sources
Beta strandi224 – 2274Combined sources
Beta strandi233 – 2364Combined sources
Beta strandi242 – 2487Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIEmodel-A101-248[»]
1R13X-ray2.10A101-248[»]
1R14X-ray2.50A101-248[»]
3PAKX-ray1.90A101-248[»]
3PAQX-ray2.10A101-248[»]
3PARX-ray2.30A101-248[»]
3PBFX-ray1.80A101-248[»]
ProteinModelPortaliP08427.
SMRiP08427. Positions 104-248.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08427.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 10073Collagen-likeAdd
BLAST
Domaini133 – 248116C-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SFTPA family.Curated
Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 collagen-like domain.Curated

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiNOG315755.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiP08427.
KOiK10067.
OMAiEMYTDGK.
OrthoDBiEOG7HXCVB.
PhylomeDBiP08427.
TreeFamiTF330481.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
[Graphical view]
PfamiPF01391. Collagen. 2 hits.
PF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08427-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLCSLAFTL FLTVVAGIKC NVTDVCAGSP GIPGAPGNHG LPGRDGRDGV
60 70 80 90 100
KGDPGPPGPM GPPGGMPGLP GRDGLPGAPG APGERGDKGE PGERGLPGFP
110 120 130 140 150
AYLDEELQTE LYEIKHQILQ TMGVLSLQGS MLSVGDKVFS TNGQSVNFDT
160 170 180 190 200
IKEMCTRAGG NIAVPRTPEE NEAIASIAKK YNNYVYLGMI EDQTPGDFHY
210 220 230 240
LDGASVNYTN WYPGEPRGQG KEKCVEMYTD GTWNDRGCLQ YRLAVCEF
Length:248
Mass (Da):26,289
Last modified:October 1, 1996 - v3
Checksum:iCAA0203009E682A5
GO

Sequence cautioni

The sequence AAA41972.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781A → G in AAA41973. (PubMed:2901856)Curated
Sequence conflicti78 – 781A → G in CAA31573. (PubMed:2901856)Curated
Sequence conflicti78 – 781A → G in CAA31574. (PubMed:2901856)Curated
Sequence conflicti84 – 841E → G in AAA41973. (PubMed:2901856)Curated
Sequence conflicti84 – 841E → G in CAA31573. (PubMed:2901856)Curated
Sequence conflicti84 – 841E → G in CAA31574. (PubMed:2901856)Curated
Sequence conflicti139 – 1391Missing in AAA41972. (PubMed:3579914)Curated
Sequence conflicti156 – 1561T → TF in AAA41972. (PubMed:3579914)Curated
Sequence conflicti180 – 1801K → N in CAA31574. (PubMed:2901856)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33201 mRNA. Translation: AAA41973.1.
X13176 mRNA. Translation: CAA31573.1.
X13177 mRNA. Translation: CAA31574.1.
M15754 mRNA. Translation: AAA41972.1. Different initiation.
U43092 Genomic DNA. Translation: AAA85516.1.
BC085353 mRNA. Translation: AAH85353.1.
PIRiA29299. LNRTPS.
RefSeqiNP_001257574.1. NM_001270645.1.
NP_001257576.1. NM_001270647.1.
NP_059025.2. NM_017329.2.
UniGeneiRn.11343.

Genome annotation databases

EnsembliENSRNOT00000047870; ENSRNOP00000039850; ENSRNOG00000011438.
GeneIDi24773.
KEGGirno:24773.
UCSCiRGD:3665. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33201 mRNA. Translation: AAA41973.1 .
X13176 mRNA. Translation: CAA31573.1 .
X13177 mRNA. Translation: CAA31574.1 .
M15754 mRNA. Translation: AAA41972.1 . Different initiation.
U43092 Genomic DNA. Translation: AAA85516.1 .
BC085353 mRNA. Translation: AAH85353.1 .
PIRi A29299. LNRTPS.
RefSeqi NP_001257574.1. NM_001270645.1.
NP_001257576.1. NM_001270647.1.
NP_059025.2. NM_017329.2.
UniGenei Rn.11343.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GIE model - A 101-248 [» ]
1R13 X-ray 2.10 A 101-248 [» ]
1R14 X-ray 2.50 A 101-248 [» ]
3PAK X-ray 1.90 A 101-248 [» ]
3PAQ X-ray 2.10 A 101-248 [» ]
3PAR X-ray 2.30 A 101-248 [» ]
3PBF X-ray 1.80 A 101-248 [» ]
ProteinModelPortali P08427.
SMRi P08427. Positions 104-248.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000039850.

Proteomic databases

PaxDbi P08427.
PRIDEi P08427.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000047870 ; ENSRNOP00000039850 ; ENSRNOG00000011438 .
GeneIDi 24773.
KEGGi rno:24773.
UCSCi RGD:3665. rat.

Organism-specific databases

CTDi 653509.
RGDi 3665. Sftpa1.

Phylogenomic databases

eggNOGi NOG315755.
GeneTreei ENSGT00700000104102.
HOGENOMi HOG000085660.
HOVERGENi HBG108270.
InParanoidi P08427.
KOi K10067.
OMAi EMYTDGK.
OrthoDBi EOG7HXCVB.
PhylomeDBi P08427.
TreeFami TF330481.

Enzyme and pathway databases

Reactomei REACT_239501. Signal regulatory protein (SIRP) family interactions.
REACT_247122. Toll Like Receptor 4 (TLR4) Cascade.

Miscellaneous databases

EvolutionaryTracei P08427.
NextBioi 604356.
PROi P08427.

Gene expression databases

Genevestigatori P08427.

Family and domain databases

Gene3Di 3.10.100.10. 1 hit.
InterProi IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
[Graphical view ]
Pfami PF01391. Collagen. 2 hits.
PF00059. Lectin_C. 1 hit.
[Graphical view ]
SMARTi SM00034. CLECT. 1 hit.
[Graphical view ]
SUPFAMi SSF56436. SSF56436. 1 hit.
PROSITEi PS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rat pulmonary surfactant protein A is expressed as two differently sized mRNA species which arise from differential polyadenylation of one transcript."
    Fisher J.H., Emrie P.A., Shannon J., Sano K., Hattler B., Mason R.J.
    Biochim. Biophys. Acta 950:338-345(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Isolation and sequence of a cDNA clone for the rat pulmonary surfactant-associated protein (PSP-A)."
    Sano K., Fisher J.H., Mason R.J., Kuroki Y., Schilling J., Benson B., Voelker D.
    Biochem. Biophys. Res. Commun. 144:367-374(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, HYDROXYLATION AT PRO-30; PRO-33; PRO-36; PRO-42; PRO-54; PRO-57; PRO-63; PRO-67; PRO-70 AND PRO-76.
  3. "Sequence of rat surfactant protein A gene and functional mapping of its upstream region."
    Smith C.I., Rosenberg E., Reisher S.R., Li F., Kefalides P., Fisher A.B., Feinstein S.I.
    Am. J. Physiol. 269:L603-L612(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Lung.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  5. "Crystal structure of trimeric carbohydrate recognition and neck domains of surfactant protein A."
    Head J.F., Mealy T.R., McCormack F.X., Seaton B.A.
    J. Biol. Chem. 278:43254-43260(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 101-248, CALCIUM-BINDING SITES, DISULFIDE BONDS.

Entry informationi

Entry nameiSFTPA_RAT
AccessioniPrimary (citable) accession number: P08427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3