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P08427

- SFTPA_RAT

UniProt

P08427 - SFTPA_RAT

Protein

Pulmonary surfactant-associated protein A

Gene

Sftpa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 3 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei21 – 211Not glycosylated
    Metal bindingi215 – 2151Calcium
    Metal bindingi217 – 2171Calcium; via carbonyl oxygen
    Metal bindingi234 – 2341Calcium
    Metal bindingi235 – 2351Calcium

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to mechanical stimulus Source: RGD
    2. cellular response to nitric oxide Source: RGD
    3. circadian rhythm Source: RGD
    4. positive regulation of phagocytosis Source: RGD
    5. respiratory gaseous exchange Source: UniProtKB-KW
    6. response to epidermal growth factor Source: RGD
    7. response to glucocorticoid Source: RGD
    8. response to hormone Source: RGD
    9. response to hyperoxia Source: RGD
    10. response to hypoxia Source: RGD
    11. response to interleukin-6 Source: RGD
    12. response to lipopolysaccharide Source: RGD
    13. response to retinoic acid Source: RGD
    14. response to vitamin A Source: RGD

    Keywords - Biological processi

    Gaseous exchange

    Keywords - Ligandi

    Calcium, Lectin, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pulmonary surfactant-associated protein A
    Short name:
    PSAP
    Short name:
    PSP-A
    Short name:
    SP-A
    Gene namesi
    Name:Sftpa1
    Synonyms:Sftp-1, Sftp1, Sftpa
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 16

    Organism-specific databases

    RGDi3665. Sftpa1.

    Subcellular locationi

    GO - Cellular componenti

    1. collagen trimer Source: UniProtKB-KW
    2. cytoplasmic vesicle Source: RGD
    3. extracellular space Source: RGD
    4. multivesicular body Source: RGD
    5. proteinaceous extracellular matrix Source: UniProtKB-SubCell
    6. rough endoplasmic reticulum Source: RGD

    Keywords - Cellular componenti

    Extracellular matrix, Secreted, Surface film

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Add
    BLAST
    Chaini21 – 248228Pulmonary surfactant-associated protein APRO_0000017462Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 – 26InterchainPROSITE-ProRule annotation
    Modified residuei30 – 3014-hydroxyproline1 Publication
    Modified residuei33 – 3314-hydroxyproline1 Publication
    Modified residuei36 – 3614-hydroxyproline1 Publication
    Modified residuei42 – 4214-hydroxyproline1 Publication
    Modified residuei54 – 5414-hydroxyproline1 Publication
    Modified residuei57 – 5714-hydroxyproline1 Publication
    Modified residuei63 – 6314-hydroxyproline1 Publication
    Modified residuei67 – 6714-hydroxyproline1 Publication
    Modified residuei70 – 7014-hydroxyproline1 Publication
    Modified residuei76 – 7614-hydroxyproline1 Publication
    Disulfide bondi155 ↔ 2461 PublicationPROSITE-ProRule annotation
    Glycosylationi207 – 2071N-linked (GlcNAc...)Curated
    Disulfide bondi224 ↔ 2381 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Hydroxylation

    Proteomic databases

    PaxDbiP08427.
    PRIDEiP08427.

    Expressioni

    Gene expression databases

    GenevestigatoriP08427.

    Interactioni

    Subunit structurei

    Oligomeric complex of 6 set of homotrimers.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000039850.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi105 – 12723
    Beta strandi132 – 1343
    Beta strandi137 – 14610
    Helixi148 – 15710
    Beta strandi160 – 1623
    Helixi168 – 18114
    Beta strandi185 – 1917
    Beta strandi198 – 2003
    Beta strandi201 – 2033
    Beta strandi224 – 2274
    Beta strandi233 – 2364
    Beta strandi242 – 2487

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GIEmodel-A101-248[»]
    1R13X-ray2.10A101-248[»]
    1R14X-ray2.50A101-248[»]
    3PAKX-ray1.90A101-248[»]
    3PAQX-ray2.10A101-248[»]
    3PARX-ray2.30A101-248[»]
    3PBFX-ray1.80A101-248[»]
    ProteinModelPortaliP08427.
    SMRiP08427. Positions 104-248.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08427.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 10073Collagen-likeAdd
    BLAST
    Domaini133 – 248116C-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SFTPA family.Curated
    Contains 1 C-type lectin domain.PROSITE-ProRule annotation
    Contains 1 collagen-like domain.Curated

    Keywords - Domaini

    Collagen, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG315755.
    GeneTreeiENSGT00700000104102.
    HOGENOMiHOG000085660.
    HOVERGENiHBG108270.
    InParanoidiP08427.
    KOiK10067.
    OMAiEMYTDGK.
    OrthoDBiEOG7HXCVB.
    PhylomeDBiP08427.
    TreeFamiTF330481.

    Family and domain databases

    Gene3Di3.10.100.10. 1 hit.
    InterProiIPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR008160. Collagen.
    [Graphical view]
    PfamiPF01391. Collagen. 2 hits.
    PF00059. Lectin_C. 1 hit.
    [Graphical view]
    SMARTiSM00034. CLECT. 1 hit.
    [Graphical view]
    SUPFAMiSSF56436. SSF56436. 1 hit.
    PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08427-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLCSLAFTL FLTVVAGIKC NVTDVCAGSP GIPGAPGNHG LPGRDGRDGV    50
    KGDPGPPGPM GPPGGMPGLP GRDGLPGAPG APGERGDKGE PGERGLPGFP 100
    AYLDEELQTE LYEIKHQILQ TMGVLSLQGS MLSVGDKVFS TNGQSVNFDT 150
    IKEMCTRAGG NIAVPRTPEE NEAIASIAKK YNNYVYLGMI EDQTPGDFHY 200
    LDGASVNYTN WYPGEPRGQG KEKCVEMYTD GTWNDRGCLQ YRLAVCEF 248
    Length:248
    Mass (Da):26,289
    Last modified:October 1, 1996 - v3
    Checksum:iCAA0203009E682A5
    GO

    Sequence cautioni

    The sequence AAA41972.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti78 – 781A → G in AAA41973. (PubMed:2901856)Curated
    Sequence conflicti78 – 781A → G in CAA31573. (PubMed:2901856)Curated
    Sequence conflicti78 – 781A → G in CAA31574. (PubMed:2901856)Curated
    Sequence conflicti84 – 841E → G in AAA41973. (PubMed:2901856)Curated
    Sequence conflicti84 – 841E → G in CAA31573. (PubMed:2901856)Curated
    Sequence conflicti84 – 841E → G in CAA31574. (PubMed:2901856)Curated
    Sequence conflicti139 – 1391Missing in AAA41972. (PubMed:3579914)Curated
    Sequence conflicti156 – 1561T → TF in AAA41972. (PubMed:3579914)Curated
    Sequence conflicti180 – 1801K → N in CAA31574. (PubMed:2901856)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33201 mRNA. Translation: AAA41973.1.
    X13176 mRNA. Translation: CAA31573.1.
    X13177 mRNA. Translation: CAA31574.1.
    M15754 mRNA. Translation: AAA41972.1. Different initiation.
    U43092 Genomic DNA. Translation: AAA85516.1.
    BC085353 mRNA. Translation: AAH85353.1.
    PIRiA29299. LNRTPS.
    RefSeqiNP_001257574.1. NM_001270645.1.
    NP_001257576.1. NM_001270647.1.
    NP_059025.2. NM_017329.2.
    UniGeneiRn.11343.

    Genome annotation databases

    EnsembliENSRNOT00000047870; ENSRNOP00000039850; ENSRNOG00000011438.
    GeneIDi24773.
    KEGGirno:24773.
    UCSCiRGD:3665. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33201 mRNA. Translation: AAA41973.1 .
    X13176 mRNA. Translation: CAA31573.1 .
    X13177 mRNA. Translation: CAA31574.1 .
    M15754 mRNA. Translation: AAA41972.1 . Different initiation.
    U43092 Genomic DNA. Translation: AAA85516.1 .
    BC085353 mRNA. Translation: AAH85353.1 .
    PIRi A29299. LNRTPS.
    RefSeqi NP_001257574.1. NM_001270645.1.
    NP_001257576.1. NM_001270647.1.
    NP_059025.2. NM_017329.2.
    UniGenei Rn.11343.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GIE model - A 101-248 [» ]
    1R13 X-ray 2.10 A 101-248 [» ]
    1R14 X-ray 2.50 A 101-248 [» ]
    3PAK X-ray 1.90 A 101-248 [» ]
    3PAQ X-ray 2.10 A 101-248 [» ]
    3PAR X-ray 2.30 A 101-248 [» ]
    3PBF X-ray 1.80 A 101-248 [» ]
    ProteinModelPortali P08427.
    SMRi P08427. Positions 104-248.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000039850.

    Proteomic databases

    PaxDbi P08427.
    PRIDEi P08427.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000047870 ; ENSRNOP00000039850 ; ENSRNOG00000011438 .
    GeneIDi 24773.
    KEGGi rno:24773.
    UCSCi RGD:3665. rat.

    Organism-specific databases

    CTDi 653509.
    RGDi 3665. Sftpa1.

    Phylogenomic databases

    eggNOGi NOG315755.
    GeneTreei ENSGT00700000104102.
    HOGENOMi HOG000085660.
    HOVERGENi HBG108270.
    InParanoidi P08427.
    KOi K10067.
    OMAi EMYTDGK.
    OrthoDBi EOG7HXCVB.
    PhylomeDBi P08427.
    TreeFami TF330481.

    Miscellaneous databases

    EvolutionaryTracei P08427.
    NextBioi 604356.
    PROi P08427.

    Gene expression databases

    Genevestigatori P08427.

    Family and domain databases

    Gene3Di 3.10.100.10. 1 hit.
    InterProi IPR001304. C-type_lectin.
    IPR016186. C-type_lectin-like.
    IPR018378. C-type_lectin_CS.
    IPR016187. C-type_lectin_fold.
    IPR008160. Collagen.
    [Graphical view ]
    Pfami PF01391. Collagen. 2 hits.
    PF00059. Lectin_C. 1 hit.
    [Graphical view ]
    SMARTi SM00034. CLECT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56436. SSF56436. 1 hit.
    PROSITEi PS00615. C_TYPE_LECTIN_1. 1 hit.
    PS50041. C_TYPE_LECTIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat pulmonary surfactant protein A is expressed as two differently sized mRNA species which arise from differential polyadenylation of one transcript."
      Fisher J.H., Emrie P.A., Shannon J., Sano K., Hattler B., Mason R.J.
      Biochim. Biophys. Acta 950:338-345(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lung.
    2. "Isolation and sequence of a cDNA clone for the rat pulmonary surfactant-associated protein (PSP-A)."
      Sano K., Fisher J.H., Mason R.J., Kuroki Y., Schilling J., Benson B., Voelker D.
      Biochem. Biophys. Res. Commun. 144:367-374(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, HYDROXYLATION AT PRO-30; PRO-33; PRO-36; PRO-42; PRO-54; PRO-57; PRO-63; PRO-67; PRO-70 AND PRO-76.
    3. "Sequence of rat surfactant protein A gene and functional mapping of its upstream region."
      Smith C.I., Rosenberg E., Reisher S.R., Li F., Kefalides P., Fisher A.B., Feinstein S.I.
      Am. J. Physiol. 269:L603-L612(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Lung.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    5. "Crystal structure of trimeric carbohydrate recognition and neck domains of surfactant protein A."
      Head J.F., Mealy T.R., McCormack F.X., Seaton B.A.
      J. Biol. Chem. 278:43254-43260(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 101-248, CALCIUM-BINDING SITES, DISULFIDE BONDS.

    Entry informationi

    Entry nameiSFTPA_RAT
    AccessioniPrimary (citable) accession number: P08427
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3