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Protein

Pulmonary surfactant-associated protein A

Gene

Sftpa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi215Calcium1
Metal bindingi217Calcium; via carbonyl oxygen1
Metal bindingi234Calcium1
Metal bindingi235Calcium1

GO - Molecular functioni

GO - Biological processi

  • cellular response to mechanical stimulus Source: RGD
  • cellular response to nitric oxide Source: RGD
  • circadian rhythm Source: RGD
  • positive regulation of phagocytosis Source: RGD
  • respiratory gaseous exchange Source: UniProtKB-KW
  • response to epidermal growth factor Source: RGD
  • response to glucocorticoid Source: RGD
  • response to hormone Source: RGD
  • response to hyperoxia Source: RGD
  • response to hypoxia Source: RGD
  • response to interleukin-6 Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to retinoic acid Source: RGD
  • response to vitamin A Source: RGD
Complete GO annotation...

Keywords - Biological processi

Gaseous exchange

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-391160. Signal regulatory protein (SIRP) family interactions.
R-RNO-5683826. Surfactant metabolism.
R-RNO-5686938. Regulation of TLR by endogenous ligand.

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein A
Short name:
PSAP
Short name:
PSP-A
Short name:
SP-A
Gene namesi
Name:Sftpa1
Synonyms:Sftp-1, Sftp1, Sftpa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi3665. Sftpa1.

Subcellular locationi

GO - Cellular componenti

  • collagen trimer Source: UniProtKB-KW
  • cytoplasmic vesicle Source: RGD
  • extracellular space Source: RGD
  • multivesicular body Source: RGD
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
  • rough endoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Add BLAST20
ChainiPRO_000001746221 – 248Pulmonary surfactant-associated protein AAdd BLAST228

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26InterchainPROSITE-ProRule annotation
Modified residuei304-hydroxyproline1 Publication1
Modified residuei334-hydroxyproline1 Publication1
Modified residuei364-hydroxyproline1 Publication1
Modified residuei424-hydroxyproline1 Publication1
Modified residuei544-hydroxyproline1 Publication1
Modified residuei574-hydroxyproline1 Publication1
Modified residuei634-hydroxyproline1 Publication1
Modified residuei674-hydroxyproline1 Publication1
Modified residuei704-hydroxyproline1 Publication1
Modified residuei764-hydroxyproline1 Publication1
Disulfide bondi155 ↔ 246PROSITE-ProRule annotation1 Publication
Glycosylationi207N-linked (GlcNAc...)Curated1
Disulfide bondi224 ↔ 238PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei21Not glycosylated1

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

PaxDbiP08427.
PRIDEiP08427.

Expressioni

Gene expression databases

BgeeiENSRNOG00000011438.
GenevisibleiP08427. RN.

Interactioni

Subunit structurei

Oligomeric complex of 6 set of homotrimers.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039850.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi109 – 127Combined sources19
Beta strandi132 – 134Combined sources3
Beta strandi137 – 146Combined sources10
Helixi148 – 157Combined sources10
Beta strandi160 – 162Combined sources3
Helixi168 – 181Combined sources14
Beta strandi188 – 190Combined sources3
Beta strandi192 – 196Combined sources5
Beta strandi198 – 200Combined sources3
Beta strandi201 – 203Combined sources3
Turni219 – 221Combined sources3
Beta strandi224 – 227Combined sources4
Beta strandi233 – 236Combined sources4
Beta strandi242 – 248Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GIEmodel-A101-248[»]
1R13X-ray2.10A101-248[»]
1R14X-ray2.50A101-248[»]
3PAKX-ray1.90A101-248[»]
3PAQX-ray2.10A101-248[»]
3PARX-ray2.30A101-248[»]
3PBFX-ray1.80A101-248[»]
4WR9X-ray2.30A101-248[»]
4WRCX-ray1.80A101-248[»]
4WREX-ray1.75A101-248[»]
4WRFX-ray1.90A101-248[»]
4WUWX-ray2.40A101-248[»]
4WUXX-ray1.90A101-248[»]
5FFRX-ray2.20A101-248[»]
5FFSX-ray1.80A101-248[»]
5FFTX-ray2.20A101-248[»]
ProteinModelPortaliP08427.
SMRiP08427.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08427.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 100Collagen-likeAdd BLAST73
Domaini133 – 248C-type lectinPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Belongs to the SFTPA family.Curated
Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 collagen-like domain.Curated

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiP08427.
KOiK10067.
OMAiTPGDFHY.
OrthoDBiEOG091G0PU5.
PhylomeDBiP08427.
TreeFamiTF330481.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR016187. CTDL_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLCSLAFTL FLTVVAGIKC NVTDVCAGSP GIPGAPGNHG LPGRDGRDGV
60 70 80 90 100
KGDPGPPGPM GPPGGMPGLP GRDGLPGAPG APGERGDKGE PGERGLPGFP
110 120 130 140 150
AYLDEELQTE LYEIKHQILQ TMGVLSLQGS MLSVGDKVFS TNGQSVNFDT
160 170 180 190 200
IKEMCTRAGG NIAVPRTPEE NEAIASIAKK YNNYVYLGMI EDQTPGDFHY
210 220 230 240
LDGASVNYTN WYPGEPRGQG KEKCVEMYTD GTWNDRGCLQ YRLAVCEF
Length:248
Mass (Da):26,289
Last modified:October 1, 1996 - v3
Checksum:iCAA0203009E682A5
GO

Sequence cautioni

The sequence AAA41972 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78A → G in AAA41973 (PubMed:2901856).Curated1
Sequence conflicti78A → G in CAA31573 (PubMed:2901856).Curated1
Sequence conflicti78A → G in CAA31574 (PubMed:2901856).Curated1
Sequence conflicti84E → G in AAA41973 (PubMed:2901856).Curated1
Sequence conflicti84E → G in CAA31573 (PubMed:2901856).Curated1
Sequence conflicti84E → G in CAA31574 (PubMed:2901856).Curated1
Sequence conflicti139Missing in AAA41972 (PubMed:3579914).Curated1
Sequence conflicti156T → TF in AAA41972 (PubMed:3579914).Curated1
Sequence conflicti180K → N in CAA31574 (PubMed:2901856).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33201 mRNA. Translation: AAA41973.1.
X13176 mRNA. Translation: CAA31573.1.
X13177 mRNA. Translation: CAA31574.1.
M15754 mRNA. Translation: AAA41972.1. Different initiation.
U43092 Genomic DNA. Translation: AAA85516.1.
BC085353 mRNA. Translation: AAH85353.1.
PIRiA29299. LNRTPS.
RefSeqiNP_001257574.1. NM_001270645.1.
NP_001257576.1. NM_001270647.1.
NP_059025.2. NM_017329.2.
UniGeneiRn.11343.

Genome annotation databases

EnsembliENSRNOT00000047870; ENSRNOP00000039850; ENSRNOG00000011438.
GeneIDi24773.
KEGGirno:24773.
UCSCiRGD:3665. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33201 mRNA. Translation: AAA41973.1.
X13176 mRNA. Translation: CAA31573.1.
X13177 mRNA. Translation: CAA31574.1.
M15754 mRNA. Translation: AAA41972.1. Different initiation.
U43092 Genomic DNA. Translation: AAA85516.1.
BC085353 mRNA. Translation: AAH85353.1.
PIRiA29299. LNRTPS.
RefSeqiNP_001257574.1. NM_001270645.1.
NP_001257576.1. NM_001270647.1.
NP_059025.2. NM_017329.2.
UniGeneiRn.11343.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GIEmodel-A101-248[»]
1R13X-ray2.10A101-248[»]
1R14X-ray2.50A101-248[»]
3PAKX-ray1.90A101-248[»]
3PAQX-ray2.10A101-248[»]
3PARX-ray2.30A101-248[»]
3PBFX-ray1.80A101-248[»]
4WR9X-ray2.30A101-248[»]
4WRCX-ray1.80A101-248[»]
4WREX-ray1.75A101-248[»]
4WRFX-ray1.90A101-248[»]
4WUWX-ray2.40A101-248[»]
4WUXX-ray1.90A101-248[»]
5FFRX-ray2.20A101-248[»]
5FFSX-ray1.80A101-248[»]
5FFTX-ray2.20A101-248[»]
ProteinModelPortaliP08427.
SMRiP08427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039850.

Proteomic databases

PaxDbiP08427.
PRIDEiP08427.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000047870; ENSRNOP00000039850; ENSRNOG00000011438.
GeneIDi24773.
KEGGirno:24773.
UCSCiRGD:3665. rat.

Organism-specific databases

CTDi653509.
RGDi3665. Sftpa1.

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiP08427.
KOiK10067.
OMAiTPGDFHY.
OrthoDBiEOG091G0PU5.
PhylomeDBiP08427.
TreeFamiTF330481.

Enzyme and pathway databases

ReactomeiR-RNO-391160. Signal regulatory protein (SIRP) family interactions.
R-RNO-5683826. Surfactant metabolism.
R-RNO-5686938. Regulation of TLR by endogenous ligand.

Miscellaneous databases

EvolutionaryTraceiP08427.
PROiP08427.

Gene expression databases

BgeeiENSRNOG00000011438.
GenevisibleiP08427. RN.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR016187. CTDL_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSFTPA_RAT
AccessioniPrimary (citable) accession number: P08427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.