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P08426 (TRY3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cationic trypsin-3

EC=3.4.21.4
Alternative name(s):
Cationic trypsin III
Pretrypsinogen III
Gene names
Name:Try3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secretedextracellular space.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processDigestion
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdigestion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515
Propeptide16 – 249Activation peptide
PRO_0000028211
Chain25 – 247223Cationic trypsin-3
PRO_0000028212

Regions

Domain25 – 245221Peptidase S1

Sites

Active site641Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2011Charge relay system By similarity
Metal binding761Calcium By similarity
Metal binding781Calcium; via carbonyl oxygen By similarity
Metal binding811Calcium; via carbonyl oxygen By similarity
Metal binding861Calcium By similarity
Site1951Required for specificity By similarity

Amino acid modifications

Disulfide bond31 ↔ 161 By similarity
Disulfide bond49 ↔ 65 By similarity
Disulfide bond133 ↔ 234 By similarity
Disulfide bond140 ↔ 207 By similarity
Disulfide bond172 ↔ 186 By similarity
Disulfide bond197 ↔ 221 By similarity

Sequences

Sequence LengthMass (Da)Tools
P08426 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: D74892BAA584E4A8

FASTA24726,269
        10         20         30         40         50         60 
MKALIFLAFL GAAVALPLDD DDDKIVGGYT CQKNSLPYQV SLNAGYHFCG GSLINSQWVV 

        70         80         90        100        110        120 
SAAHCYKSRI QVRLGEHNID VVEGGEQFID AAKIIRHPSY NANTFDNDIM LIKLNSPATL 

       130        140        150        160        170        180 
NSRVSTVSLP RSCGSSGTKC LVSGWGNTLS SGTNYPSLLQ CLDAPVLSDS SCKSSYPGKI 

       190        200        210        220        230        240 
TSNMFCLGFL EGGKDSCQGD SGGPVVCNGQ LQGVVSWGYG CAQKGKPGVY TKVCNYVNWI 


QQTVAAN 

« Hide

References

[1]"Isolation and characterization of a cDNA encoding rat cationic trypsinogen."
Fletcher T.S., Alhadeff M., Craik C.S., Largman C.
Biochemistry 26:3081-3086(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16624 mRNA. Translation: AAA41985.1.
PIRA27547.
RefSeqNP_775150.1. NM_173127.1.
UniGeneRn.9817.

3D structure databases

ProteinModelPortalP08426.
SMRP08426. Positions 25-247.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000018042.

Protein family/group databases

MEROPSS01.151.

PTM databases

PhosphoSiteP08426.

Proteomic databases

PaxDbP08426.
PRIDEP08426.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID286911.
KEGGrno:286911.
UCSCRGD:708437. rat.

Organism-specific databases

CTD5646.
RGD708437. LOC286911.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP08426.
KOK01312.
PhylomeDBP08426.

Gene expression databases

GenevestigatorP08426.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio625019.

Entry information

Entry nameTRY3_RAT
AccessionPrimary (citable) accession number: P08426
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 14, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries