Reviewed, UniProtKB/Swiss-Prot P08426 (TRY3_RAT)

Names and origin

Protein names

Recommended name:
    Cationic trypsin-3
    EC=3.4.21.4
Alternative name(s):
    Cationic trypsin III
    Pretrypsinogen III

Gene names

Name:

Try3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 24	9	Activation peptide		PRO_0000028211
	Chain	25 – 247	223	Cationic trypsin-3		PRO_0000028212
Regions
	Domain	25 – 245	221	Peptidase S1
Sites
	Active site	64	1	Charge relay system By similarity
	Active site	108	1	Charge relay system By similarity
	Active site	201	1	Charge relay system By similarity
	Metal binding	76	1	Calcium By similarity
	Metal binding	78	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	81	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	86	1	Calcium By similarity
	Site	195	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	31 ↔ 161		By similarity
	Disulfide bond	49 ↔ 65		By similarity
	Disulfide bond	133 ↔ 234		By similarity
	Disulfide bond	140 ↔ 207		By similarity
	Disulfide bond	172 ↔ 186		By similarity
	Disulfide bond	197 ↔ 221		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08426-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: D74892BAA584E4A8	FASTA	247	26,269
	10 20 30 40 50 60 MKALIFLAFL GAAVALPLDD DDDKIVGGYT CQKNSLPYQV SLNAGYHFCG GSLINSQWVV 70 80 90 100 110 120 SAAHCYKSRI QVRLGEHNID VVEGGEQFID AAKIIRHPSY NANTFDNDIM LIKLNSPATL 130 140 150 160 170 180 NSRVSTVSLP RSCGSSGTKC LVSGWGNTLS SGTNYPSLLQ CLDAPVLSDS SCKSSYPGKI 190 200 210 220 230 240 TSNMFCLGFL EGGKDSCQGD SGGPVVCNGQ LQGVVSWGYG CAQKGKPGVY TKVCNYVNWI QQTVAAN

References

[1]	"Isolation and characterization of a cDNA encoding rat cationic trypsinogen." Fletcher T.S., Alhadeff M., Craik C.S., Largman C. Biochemistry 26:3081-3086(1987) [PubMed: 3607011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16624 mRNA. Translation: AAA41985.1.
IPI	IPI00211212.
PIR	A27547.
RefSeq	NP_775150.1.
UniGene	Rn.9817
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
SMR	P08426. Positions 25-247.
ModBase	Search...
Protein family/group databases
MEROPS	S01.151.
Proteomic databases
PRIDE	P08426.
Genome annotation databases
Ensembl	ENSRNOG00000013382. Rattus norvegicus. [Contig view]
GeneID	286911.
KEGG	rno:286911.
Organism-specific databases
RGD	708437. LOC286911.
Phylogenomic databases
HOVERGEN	P08426.
Enzyme and pathway databases
BRENDA	3.4.21.4. 248.
Gene expression databases
ArrayExpress	P08426.
GermOnline	ENSRNOG00000013382. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	625019.

Entry information

Entry name

TRY3_RAT

Accession

Primary (citable) accession number: P08426

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)