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Protein

Phenylalanine--tRNA ligase, mitochondrial

Gene

MSF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation.

Miscellaneous

Present with 1800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155SubstrateBy similarity1
Binding sitei302Substrate; via carbonyl oxygenBy similarity1
Binding sitei329Substrate; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phenylalanine-tRNA ligase activity Source: UniProtKB
  • tRNA binding Source: InterPro

GO - Biological processi

  • mitochondrial phenylalanyl-tRNA aminoacylation Source: SGD
  • phenylalanyl-tRNA aminoacylation Source: UniProtKB

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34202-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase, mitochondrial (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase
Short name:
PheRS
Gene namesi
Name:MSF1
Ordered Locus Names:YPR047W
ORF Names:YP9499.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR047W
SGDiS000006251 MSF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionAdd BLAST17
ChainiPRO_000003581718 – 469Phenylalanine--tRNA ligase, mitochondrialAdd BLAST452

Proteomic databases

MaxQBiP08425
PaxDbiP08425
PRIDEiP08425

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi36223, 87 interactors
DIPiDIP-3901N
IntActiP08425, 1 interactor
MINTiP08425
STRINGi4932.YPR047W

Structurei

3D structure databases

ProteinModelPortaliP08425
SMRiP08425
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini372 – 469FDX-ACBPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 129Substrate bindingBy similarity4
Regioni162 – 164Substrate bindingBy similarity3
Regioni169 – 171Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063467
HOGENOMiHOG000165163
InParanoidiP08425
KOiK01889
OMAiTYFPFTQ
OrthoDBiEOG092C2OPN

Family and domain databases

Gene3Di3.30.70.380, 1 hit
InterProiView protein in InterPro
IPR006195 aa-tRNA-synth_II
IPR005121 Fdx_antiC-bd
IPR036690 Fdx_antiC-bd_sf
IPR004530 Phe-tRNA-synth_IIc_mito
IPR002319 Phenylalanyl-tRNA_Synthase
PANTHERiPTHR11538:SF41 PTHR11538:SF41, 1 hit
PfamiView protein in Pfam
PF03147 FDX-ACB, 1 hit
PF01409 tRNA-synt_2d, 2 hits
SMARTiView protein in SMART
SM00896 FDX-ACB, 1 hit
SUPFAMiSSF54991 SSF54991, 1 hit
TIGRFAMsiTIGR00469 pheS_mito, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit
PS51447 FDX_ACB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLNRMMKTR TGLYRLYSTL KVPHVEINGI KYKTDPQTTN VTDSIIKLTD
60 70 80 90 100
RSLHLKESHP VGILRDLIEK KLNSVDNTFK IFNNFKPVVT TMENFDSLGF
110 120 130 140 150
PKDHPGRSKS DTYYINETHL LRTHTSAHEL ECFQKIRNDS DNIKSGFLIS
160 170 180 190 200
ADVYRRDEID KTHYPVFHQM EGATIWKRTK ADVGVKEPMY IEKIREDIRQ
210 220 230 240 250
VENLLNKENV KITVDDDTIP LKENNPKQEY MSDLEVDLCS QHLKRSIELI
260 270 280 290 300
VSEVFNKKIS SMIKNKANNT PKELKVRWIN AYFPWTAPSW EIEVWWQGEW
310 320 330 340 350
LELCGCGLIR QDVLLRAGYK PSETIGWAFG LGLDRIAMLL FEIPDIRLLW
360 370 380 390 400
SRDERFSRQF SKGLITSFKP YSKHPGSFRD VAFWLPEDKP DIHQVHENDL
410 420 430 440 450
MEIIRNIAGD LVESVKLVDS FTHPKTGRKS MCYRINYQSM DRNLTNAEVN
460
TLQDMVCSKL VKEYSVELR
Length:469
Mass (Da):54,829
Last modified:September 5, 2006 - v3
Checksum:iE728783D76C74C18
GO

Sequence cautioni

The sequence AAA34800 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA89167 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA94994 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86K → E in AAA34800 (PubMed:3029120).Curated1
Sequence conflicti233D → H in AAA34800 (PubMed:3029120).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02691 Genomic DNA Translation: AAA34800.1 Different initiation.
Z71255 Genomic DNA Translation: CAA94994.1 Different initiation.
Z49219 Genomic DNA Translation: CAA89167.1 Different initiation.
BK006949 Genomic DNA Translation: DAA11471.1
PIRiS54071 YFBYAM
RefSeqiNP_015372.2, NM_001184144.1

Genome annotation databases

EnsemblFungiiYPR047W; YPR047W; YPR047W
GeneIDi856160
KEGGisce:YPR047W

Similar proteinsi

Entry informationi

Entry nameiSYFM_YEAST
AccessioniPrimary (citable) accession number: P08425
Secondary accession number(s): D6W455, Q12410
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 171 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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