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P08424

- RENI_RAT

UniProt

P08424 - RENI_RAT

Protein

Renin

Gene

Ren1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

    Catalytic activityi

    Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

    Enzyme regulationi

    Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei102 – 1021PROSITE-ProRule annotation
    Active sitei287 – 2871PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. endopeptidase activity Source: RGD
    3. insulin-like growth factor receptor binding Source: RGD
    4. receptor binding Source: RGD

    GO - Biological processi

    1. angiotensin maturation Source: RGD
    2. beta-amyloid metabolic process Source: RGD
    3. cell maturation Source: Ensembl
    4. cellular response to drug Source: RGD
    5. drinking behavior Source: Ensembl
    6. hormone-mediated signaling pathway Source: Ensembl
    7. male gonad development Source: Ensembl
    8. mesonephros development Source: Ensembl
    9. regulation of blood pressure Source: RGD
    10. regulation of MAPK cascade Source: Ensembl
    11. renin-angiotensin regulation of aldosterone production Source: Ensembl
    12. response to cAMP Source: RGD
    13. response to cGMP Source: RGD
    14. response to drug Source: RGD
    15. response to immobilization stress Source: RGD
    16. response to lipopolysaccharide Source: RGD

    Keywords - Molecular functioni

    Aspartyl protease, Hydrolase, Protease

    Enzyme and pathway databases

    ReactomeiREACT_198807. Metabolism of Angiotensinogen to Angiotensins.

    Protein family/group databases

    MEROPSiA01.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Renin (EC:3.4.23.15)
    Alternative name(s):
    Angiotensinogenase
    Gene namesi
    Name:Ren1
    Synonyms:Ren
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi3554. Ren1.

    Subcellular locationi

    Secreted By similarity. Membrane By similarity
    Note: Associated to membranes via binding to ATP6AP2.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. extracellular space Source: RGD
    3. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626CuratedAdd
    BLAST
    Propeptidei27 – 6438Activation peptidePRO_0000026091Add
    BLAST
    Chaini65 – 402338ReninPRO_0000026092Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi115 ↔ 122By similarity
    Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi278 ↔ 282By similarity
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    PTM databases

    PhosphoSiteiP08424.

    Expressioni

    Gene expression databases

    GenevestigatoriP08424.

    Interactioni

    Subunit structurei

    Interacts with ATP6AP2.By similarity

    Protein-protein interaction databases

    BioGridi246843. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP08424.
    SMRiP08424. Positions 68-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase A1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG248684.
    GeneTreeiENSGT00710000106265.
    HOGENOMiHOG000197681.
    HOVERGENiHBG000482.
    InParanoidiP08424.
    KOiK01380.
    OMAiGSDPAYY.
    OrthoDBiEOG7HQN88.
    PhylomeDBiP08424.
    TreeFamiTF314990.

    Family and domain databases

    Gene3Di2.40.70.10. 2 hits.
    InterProiIPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR012848. Aspartic_peptidase_N.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view]
    PANTHERiPTHR13683. PTHR13683. 1 hit.
    PfamiPF07966. A1_Propeptide. 1 hit.
    PF00026. Asp. 1 hit.
    [Graphical view]
    PRINTSiPR00792. PEPSIN.
    SUPFAMiSSF50630. SSF50630. 1 hit.
    PROSITEiPS00141. ASP_PROTEASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08424-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGGRRMPLWA LLLLWTSCSF SLPTDTASFG RILLKKMPSV REILEERGVD    50
    MTRISAEWGE FIKKSSFTNV TSPVVLTNYL DTQYYGEIGI GTPSQTFKVI 100
    FDTGSANLWV PSTKCGPLYT ACEIHNLYDS SESSSYMENG TEFTIHYGSG 150
    KVKGFLSQDV VTVGGIIVTQ TFGEVTELPL IPFMLAKFDG VLGMGFPAQA 200
    VDGVIPVFDH ILSQRVLKEE VFSVYYSRES HLLGGEVVLG GSDPQHYQGN 250
    FHYVSISKAG SWQITMKGVS VGPATLLCEE GCMAVVDTGT SYISGPTSSL 300
    QLIMQALGVK EKRANNYVVN CSQVPTLPDI SFYLGGRTYT LSNMDYVQKN 350
    PFRNDDLCIL ALQGLDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL 400
    AR 402
    Length:402
    Mass (Da):44,276
    Last modified:February 1, 1996 - v2
    Checksum:iE4E22CEE449EAC3F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti200 – 2001A → V in CAA30082. (PubMed:3287330)Curated
    Sequence conflicti214 – 2152QR → HE in AAA42030. (PubMed:3039496)Curated
    Sequence conflicti271 – 2711V → L in CAA30082. (PubMed:3287330)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07033 mRNA. Translation: CAA30082.1.
    J02941 mRNA. Translation: AAA42030.1.
    M37278 Genomic DNA. Translation: AAA42031.1. Sequence problems.
    S60054 mRNA. Translation: AAP13916.1.
    BC078878 mRNA. Translation: AAH78878.1.
    PIRiA29991. RERTK.
    RefSeqiNP_036774.4. NM_012642.4.
    UniGeneiRn.9831.

    Genome annotation databases

    EnsembliENSRNOT00000003951; ENSRNOP00000003951; ENSRNOG00000002937.
    GeneIDi24715.
    KEGGirno:24715.
    UCSCiRGD:3554. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07033 mRNA. Translation: CAA30082.1 .
    J02941 mRNA. Translation: AAA42030.1 .
    M37278 Genomic DNA. Translation: AAA42031.1 . Sequence problems.
    S60054 mRNA. Translation: AAP13916.1 .
    BC078878 mRNA. Translation: AAH78878.1 .
    PIRi A29991. RERTK.
    RefSeqi NP_036774.4. NM_012642.4.
    UniGenei Rn.9831.

    3D structure databases

    ProteinModelPortali P08424.
    SMRi P08424. Positions 68-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246843. 1 interaction.

    Chemistry

    BindingDBi P08424.
    ChEMBLi CHEMBL2322.

    Protein family/group databases

    MEROPSi A01.007.

    PTM databases

    PhosphoSitei P08424.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000003951 ; ENSRNOP00000003951 ; ENSRNOG00000002937 .
    GeneIDi 24715.
    KEGGi rno:24715.
    UCSCi RGD:3554. rat.

    Organism-specific databases

    CTDi 5972.
    RGDi 3554. Ren1.

    Phylogenomic databases

    eggNOGi NOG248684.
    GeneTreei ENSGT00710000106265.
    HOGENOMi HOG000197681.
    HOVERGENi HBG000482.
    InParanoidi P08424.
    KOi K01380.
    OMAi GSDPAYY.
    OrthoDBi EOG7HQN88.
    PhylomeDBi P08424.
    TreeFami TF314990.

    Enzyme and pathway databases

    Reactomei REACT_198807. Metabolism of Angiotensinogen to Angiotensins.

    Miscellaneous databases

    NextBioi 604209.
    PROi P08424.

    Gene expression databases

    Genevestigatori P08424.

    Family and domain databases

    Gene3Di 2.40.70.10. 2 hits.
    InterProi IPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR012848. Aspartic_peptidase_N.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view ]
    PANTHERi PTHR13683. PTHR13683. 1 hit.
    Pfami PF07966. A1_Propeptide. 1 hit.
    PF00026. Asp. 1 hit.
    [Graphical view ]
    PRINTSi PR00792. PEPSIN.
    SUPFAMi SSF50630. SSF50630. 1 hit.
    PROSITEi PS00141. ASP_PROTEASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Sprague-Dawley.
    4. "Renin gene nucleotide sequence of coding and regulatory regions in Dahl rats."
      Alam K.Y., Wang Y., Dene H., Rapp J.P.
      Clin. Exp. Hypertens. 15:599-614(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiRENI_RAT
    AccessioniPrimary (citable) accession number: P08424
    Secondary accession number(s): Q63497
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3