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Protein

Renin

Gene

Ren1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activityi

Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Enzyme regulationi

Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102PROSITE-ProRule annotation1
Active sitei287PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: RGD
  • endopeptidase activity Source: RGD
  • insulin-like growth factor receptor binding Source: RGD
  • peptidase activity Source: RGD
  • receptor binding Source: RGD

GO - Biological processi

  • amyloid-beta metabolic process Source: RGD
  • angiotensin maturation Source: RGD
  • cell maturation Source: RGD
  • cellular response to drug Source: RGD
  • drinking behavior Source: RGD
  • hormone-mediated signaling pathway Source: RGD
  • kidney development Source: RGD
  • male gonad development Source: RGD
  • mesonephros development Source: RGD
  • proteolysis Source: RGD
  • regulation of blood pressure Source: RGD
  • regulation of blood volume by renin-angiotensin Source: RGD
  • regulation of MAPK cascade Source: RGD
  • renin-angiotensin regulation of aldosterone production Source: RGD
  • response to cAMP Source: RGD
  • response to cGMP Source: RGD
  • response to drug Source: RGD
  • response to immobilization stress Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to organic substance Source: RGD

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-RNO-2022377. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiA01.008.

Names & Taxonomyi

Protein namesi
Recommended name:
Renin (EC:3.4.23.15)
Alternative name(s):
Angiotensinogenase
Gene namesi
Name:Ren1
Synonyms:Ren
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi3554. Ren1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2322.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26CuratedAdd BLAST26
PropeptideiPRO_000002609127 – 64Activation peptideAdd BLAST38
ChainiPRO_000002609265 – 402ReninAdd BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi69N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi115 ↔ 122By similarity
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi278 ↔ 282By similarity
Glycosylationi320N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP08424.

PTM databases

PhosphoSitePlusiP08424.

Expressioni

Gene expression databases

BgeeiENSRNOG00000002937.
ExpressionAtlasiP08424. baseline and differential.
GenevisibleiP08424. RN.

Interactioni

Subunit structurei

Interacts with ATP6AP2.By similarity

GO - Molecular functioni

  • insulin-like growth factor receptor binding Source: RGD
  • receptor binding Source: RGD

Protein-protein interaction databases

BioGridi246843. 1 interactor.
STRINGi10116.ENSRNOP00000003951.

Chemistry databases

BindingDBiP08424.

Structurei

Secondary structure

1402
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 36Combined sources7
Helixi40 – 46Combined sources7
Helixi51 – 58Combined sources8
Beta strandi79 – 81Combined sources3
Beta strandi84 – 90Combined sources7
Turni91 – 94Combined sources4
Beta strandi95 – 102Combined sources8
Beta strandi108 – 112Combined sources5
Helixi120 – 124Combined sources5
Helixi130 – 132Combined sources3
Beta strandi137 – 146Combined sources10
Beta strandi151 – 163Combined sources13
Beta strandi166 – 177Combined sources12
Helixi180 – 183Combined sources4
Beta strandi190 – 193Combined sources4
Helixi197 – 199Combined sources3
Helixi201 – 203Combined sources3
Helixi207 – 213Combined sources7
Beta strandi217 – 226Combined sources10
Beta strandi235 – 241Combined sources7
Helixi244 – 246Combined sources3
Beta strandi247 – 255Combined sources9
Beta strandi261 – 271Combined sources11
Beta strandi274 – 277Combined sources4
Beta strandi282 – 286Combined sources5
Beta strandi292 – 295Combined sources4
Helixi297 – 307Combined sources11
Beta strandi316 – 318Combined sources3
Helixi321 – 323Combined sources3
Beta strandi330 – 334Combined sources5
Beta strandi337 – 341Combined sources5
Turni343 – 345Combined sources3
Beta strandi346 – 348Combined sources3
Beta strandi359 – 364Combined sources6
Turni369 – 371Combined sources3
Beta strandi373 – 377Combined sources5
Helixi379 – 384Combined sources6
Beta strandi385 – 390Combined sources6
Turni391 – 394Combined sources4
Beta strandi395 – 401Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MLGX-ray2.60A22-402[»]
ProteinModelPortaliP08424.
SMRiP08424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 399Peptidase A1PROSITE-ProRule annotationAdd BLAST316

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP08424.
KOiK01380.
OMAiLGKYYTE.
OrthoDBiEOG091G0JP7.
PhylomeDBiP08424.
TreeFamiTF314990.

Family and domain databases

CDDicd05487. renin_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom_sf.
IPR034135. Renin-like_dom.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiView protein in Pfam
PF00026. Asp. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGRRMPLWA LLLLWTSCSF SLPTDTASFG RILLKKMPSV REILEERGVD
60 70 80 90 100
MTRISAEWGE FIKKSSFTNV TSPVVLTNYL DTQYYGEIGI GTPSQTFKVI
110 120 130 140 150
FDTGSANLWV PSTKCGPLYT ACEIHNLYDS SESSSYMENG TEFTIHYGSG
160 170 180 190 200
KVKGFLSQDV VTVGGIIVTQ TFGEVTELPL IPFMLAKFDG VLGMGFPAQA
210 220 230 240 250
VDGVIPVFDH ILSQRVLKEE VFSVYYSRES HLLGGEVVLG GSDPQHYQGN
260 270 280 290 300
FHYVSISKAG SWQITMKGVS VGPATLLCEE GCMAVVDTGT SYISGPTSSL
310 320 330 340 350
QLIMQALGVK EKRANNYVVN CSQVPTLPDI SFYLGGRTYT LSNMDYVQKN
360 370 380 390 400
PFRNDDLCIL ALQGLDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL

AR
Length:402
Mass (Da):44,276
Last modified:February 1, 1996 - v2
Checksum:iE4E22CEE449EAC3F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200A → V in CAA30082 (PubMed:3287330).Curated1
Sequence conflicti214 – 215QR → HE in AAA42030 (PubMed:3039496).Curated2
Sequence conflicti271V → L in CAA30082 (PubMed:3287330).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07033 mRNA. Translation: CAA30082.1.
J02941 mRNA. Translation: AAA42030.1.
M37278 Genomic DNA. Translation: AAA42031.1. Sequence problems.
S60054 mRNA. Translation: AAP13916.1.
BC078878 mRNA. Translation: AAH78878.1.
PIRiA29991. RERTK.
RefSeqiNP_036774.4. NM_012642.4.
UniGeneiRn.9831.

Genome annotation databases

EnsembliENSRNOT00000003951; ENSRNOP00000003951; ENSRNOG00000002937.
GeneIDi24715.
KEGGirno:24715.
UCSCiRGD:3554. rat.

Similar proteinsi

Entry informationi

Entry nameiRENI_RAT
AccessioniPrimary (citable) accession number: P08424
Secondary accession number(s): Q63497
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome