Renin precursor - Rattus norvegicus (Rat)

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P08424 (RENI_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 123. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Renin
EC=3.4.23.15

Alternative name(s):
Angiotensinogenase

Gene names

Name:	Ren1
Synonyms:	Ren

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Catalytic activity	Cleavage of Leu-\|-Xaa bond in angiotensinogen to generate angiotensin I.
Enzyme regulation	Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing By similarity.
Subunit structure	Interacts with ATP6AP2 By similarity.
Subcellular location	Secreted By similarity. Membrane By similarity. Note: Associated to membranes via binding to ATP6AP2 By similarity.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Membrane Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiotensin maturation Inferred from direct assay PubMed 21321306. Source: RGD beta-amyloid metabolic process Inferred from expression pattern PubMed 22609375. Source: RGD cell maturation Inferred from electronic annotation. Source: Compara cellular response to drug Inferred from expression pattern PubMed 21963836. Source: RGD drinking behavior Inferred from electronic annotation. Source: Compara hormone-mediated signaling pathway Inferred from electronic annotation. Source: Compara male gonad development Inferred from electronic annotation. Source: Compara mesonephros development Inferred from electronic annotation. Source: Compara proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of MAPK cascade Inferred from electronic annotation. Source: Compara renin-angiotensin regulation of aldosterone production Inferred from electronic annotation. Source: Compara response to cAMP Inferred from direct assay PubMed 9671794. Source: RGD response to cGMP Inferred from direct assay PubMed 9671794. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 22342485. Source: RGD response to stress Inferred from direct assay PubMed 7042704. Source: RGD
Cellular_component	cytoplasmic part Inferred from direct assay PubMed 12242043. Source: RGD extracellular space Inferred from direct assay PubMed 1278112. Source: RGD membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW endopeptidase activity Inferred from direct assay PubMed 7060565. Source: RGD insulin-like growth factor receptor binding Inferred from direct assay PubMed 11247783. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Probable

Propeptide

27 – 64

Activation peptide

PRO_0000026091

Chain

65 – 402

338

Renin

PRO_0000026092

Sites

Active site

102

By similarity

Active site

287

By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

139

N-linked (GlcNAc...) Potential

Glycosylation

320

N-linked (GlcNAc...) Potential

Disulfide bond

115 ↔ 122

By similarity

Disulfide bond

278 ↔ 282

By similarity

Experimental info

Sequence conflict

200

A → V in CAA30082. Ref.1

Sequence conflict

214 – 215

QR → HE in AAA42030. Ref.2

Sequence conflict

271

V → L in CAA30082. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P08424 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E4E22CEE449EAC3F
Show »

FASTA

402

44,276

        10         20         30         40         50         60 
MGGRRMPLWA LLLLWTSCSF SLPTDTASFG RILLKKMPSV REILEERGVD MTRISAEWGE 

        70         80         90        100        110        120 
FIKKSSFTNV TSPVVLTNYL DTQYYGEIGI GTPSQTFKVI FDTGSANLWV PSTKCGPLYT 

       130        140        150        160        170        180 
ACEIHNLYDS SESSSYMENG TEFTIHYGSG KVKGFLSQDV VTVGGIIVTQ TFGEVTELPL 

       190        200        210        220        230        240 
IPFMLAKFDG VLGMGFPAQA VDGVIPVFDH ILSQRVLKEE VFSVYYSRES HLLGGEVVLG 

       250        260        270        280        290        300 
GSDPQHYQGN FHYVSISKAG SWQITMKGVS VGPATLLCEE GCMAVVDTGT SYISGPTSSL 

       310        320        330        340        350        360 
QLIMQALGVK EKRANNYVVN CSQVPTLPDI SFYLGGRTYT LSNMDYVQKN PFRNDDLCIL 

       370        380        390        400 
ALQGLDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL AR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of rat renin cDNA." Tada M., Fukamizu A., Seo M.S., Takahashi S., Murakami K. Nucleic Acids Res. 16:3576-3576(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"Molecular cloning of rat renin cDNA and its gene." Burnham C.E., Hawelu-Johnson C.L., Frank B.M., Lynch K.R. Proc. Natl. Acad. Sci. U.S.A. 84:5605-5609(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure of the rat renin gene." Fukamizu A., Nishi K., Cho T., Saitoh M., Nakayama K., Ohkubo H., Nakanishi S., Murakami K. J. Mol. Biol. 201:443-450(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley.
[4]	"Renin gene nucleotide sequence of coding and regulatory regions in Dahl rats." Alam K.Y., Wang Y., Dene H., Rapp J.P. Clin. Exp. Hypertens. 15:599-614(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07033 mRNA. Translation: CAA30082.1. J02941 mRNA. Translation: AAA42030.1. M37278 Genomic DNA. Translation: AAA42031.1. Sequence problems. S60054 mRNA. Translation: AAP13916.1. BC078878 mRNA. Translation: AAH78878.1.
IPI	IPI00211210.
PIR	RERTK. A29991.
RefSeq	NP_036774.4. NM_012642.4.
UniGene	Rn.9831.
3D structure databases
ProteinModelPortal	P08424.
SMR	P08424. Positions 68-402.
ModBase	Search...
Protein family/group databases
MEROPS	A01.007.
PTM databases
PhosphoSite	P08424.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003951; ENSRNOP00000003951; ENSRNOG00000002937.
GeneID	24715.
KEGG	rno:24715.
UCSC	RGD:3554. rat.
Organism-specific databases
CTD	5972.
RGD	3554. Ren1.
Phylogenomic databases
eggNOG	NOG248684.
GeneTree	ENSGT00700000104424.
HOGENOM	HOG000197681.
HOVERGEN	HBG000482.
InParanoid	P08424.
KO	K01380.
OMA	DPQHYQG.
OrthoDB	EOG4W3SN8.
Gene expression databases
ArrayExpress	P08424.
Genevestigator	P08424.
GermOnline	ENSRNOG00000002937. Rattus norvegicus.
Family and domain databases
Gene3D	2.40.70.10. 2 hits.
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR012848. Propep_A1. [Graphical view]
PANTHER	PTHR13683. PTHR13683. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P08424.
ChEMBL	CHEMBL2322.
NextBio	604209.

Entry information

Entry name

RENI_RAT

Accession

Primary (citable) accession number: P08424
Secondary accession number(s): Q63497

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents