Reviewed,
UniProtKB/Swiss-Prot P08424 (RENI_RAT)
Last modified
June 16, 2009.
Version 92.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Renin EC=3.4.23.15 Alternative name(s): Angiotensinogenase | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 402 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. |
| Catalytic activity | Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I. |
| Enzyme regulation | Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing By similarity. |
| Subunit structure | Interacts with ATP6AP2 By similarity. |
| Subcellular location | Secreted By similarity. Membrane By similarity. Note: Associated to membranes via binding to ATP6AP2 By similarity. |
| Sequence similarities | Belongs to the peptidase A1 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane Secreted |
| Domain | Signal |
| Molecular function | Aspartyl protease Hydrolase Protease |
| PTM | Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasmic part Inferred from direct assay. Source: RGD membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW insulin-like growth factor receptor bindingInferred from direct assay. Source: RGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 26 | 26 | Probable | ||||||||
| Propeptide | 27 – 64 | 38 | Activation peptide | PRO_0000026091 | |||||||
| Chain | 65 – 402 | 338 | Renin | PRO_0000026092 | |||||||
Sites | |||||||||||
| Active site | 102 | 1 | By similarity | ||||||||
| Active site | 287 | 1 | By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 69 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 139 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 320 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 115 ↔ 122 | By similarity | |||||||||
| Disulfide bond | 278 ↔ 282 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 200 | 1 | A → V in CAA30082. Ref.1 | ||||||||
| Sequence conflict | 214 – 215 | 2 | QR → HE in AAA42030. Ref.2 | ||||||||
| Sequence conflict | 271 | 1 | V → L in CAA30082. Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of rat renin cDNA." Tada M., Fukamizu A., Seo M.S., Takahashi S., Murakami K. Nucleic Acids Res. 16:3576-3576(1988) [PubMed: 3287330] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. |
| [2] | "Molecular cloning of rat renin cDNA and its gene." Burnham C.E., Hawelu-Johnson C.L., Frank B.M., Lynch K.R. Proc. Natl. Acad. Sci. U.S.A. 84:5605-5609(1987) [PubMed: 3039496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Structure of the rat renin gene." Fukamizu A., Nishi K., Cho T., Saitoh M., Nakayama K., Ohkubo H., Nakanishi S., Murakami K. J. Mol. Biol. 201:443-450(1988) [PubMed: 3047403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley. |
| [4] | "Renin gene nucleotide sequence of coding and regulatory regions in Dahl rats." Alam K.Y., Wang Y., Dene H., Rapp J.P. Clin. Exp. Hypertens. 15:599-614(1993) [PubMed: 8490598] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
Cross-references
Sequence databases | |
|---|---|
| X07033 mRNA. Translation: CAA30082.1. J02941 mRNA. Translation: AAA42030.1. M37278 Genomic DNA. Translation: AAA42031.1. Sequence problems. S60054 mRNA. Translation: AAP13916.1. BC078878 mRNA. Translation: AAH78878.1. | |
| IPI | IPI00211210. |
| PIR | RERTK. A29991. |
| RefSeq | NP_036774.4. |
| UniGene | Rn.9831 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1SMR based on UniProtKB P00796. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | A01.007. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000002937. Rattus norvegicus. [Contig view] |
| GeneID | 24715. |
| KEGG | rno:24715. |
Organism-specific databases | |
| RGD | 3554. Ren1. |
Phylogenomic databases | |
| HOVERGEN | P08424. |
| OMA | P08424. DPQHYQG. |
Enzyme and pathway databases | |
| BRENDA | 3.4.23.15. 248. |
Gene expression databases | |
| ArrayExpress | P08424. |
| GermOnline | ENSRNOG00000002937. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view] |
| Gene3D | G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit. |
| PANTHER | PTHR13683. Peptidase_A1. 1 hit. |
| Pfam | PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view] |
| PRINTS | PR00792. PEPSIN. |
| PROSITE | PS00141. ASP_PROTEASE. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 604209. |
Entry information
| Entry name | RENI_RAT | ||||||||
| Accession | Primary (citable) accession number: P08424 Secondary accession number(s): Q63497 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


