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Reviewed, UniProtKB/Swiss-Prot P08424 (RENI_RAT)

Last modified June 16, 2009. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Renin
    EC=3.4.23.15
Alternative name(s):
    Angiotensinogenase
Gene names
Name: Ren1
Synonyms: Ren
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length402 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activity

Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Enzyme regulation

Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing By similarity.

Subunit structure

Interacts with ATP6AP2 By similarity.

Subcellular location

Secreted By similarity. Membrane By similarity. Note: Associated to membranes via binding to ATP6AP2 By similarity.

Sequence similarities

Belongs to the peptidase A1 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Probable
Propeptide27 – 6438Activation peptide
PRO_0000026091
Chain65 – 402338Renin
PRO_0000026092

Sites

Active site1021 By similarity
Active site2871 By similarity

Amino acid modifications

Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation1391N-linked (GlcNAc...) Potential
Glycosylation3201N-linked (GlcNAc...) Potential
Disulfide bond115 ↔ 122 By similarity
Disulfide bond278 ↔ 282 By similarity

Experimental info

Sequence conflict2001A → V in CAA30082. Ref.1
Sequence conflict214 – 2152QR → HE in AAA42030. Ref.2
Sequence conflict2711V → L in CAA30082. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P08424-1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E4E22CEE449EAC3F

FASTA40244,276
        10         20         30         40         50         60 
MGGRRMPLWA LLLLWTSCSF SLPTDTASFG RILLKKMPSV REILEERGVD MTRISAEWGE 

        70         80         90        100        110        120 
FIKKSSFTNV TSPVVLTNYL DTQYYGEIGI GTPSQTFKVI FDTGSANLWV PSTKCGPLYT 

       130        140        150        160        170        180 
ACEIHNLYDS SESSSYMENG TEFTIHYGSG KVKGFLSQDV VTVGGIIVTQ TFGEVTELPL 

       190        200        210        220        230        240 
IPFMLAKFDG VLGMGFPAQA VDGVIPVFDH ILSQRVLKEE VFSVYYSRES HLLGGEVVLG 

       250        260        270        280        290        300 
GSDPQHYQGN FHYVSISKAG SWQITMKGVS VGPATLLCEE GCMAVVDTGT SYISGPTSSL 

       310        320        330        340        350        360 
QLIMQALGVK EKRANNYVVN CSQVPTLPDI SFYLGGRTYT LSNMDYVQKN PFRNDDLCIL 

       370        380        390        400 
ALQGLDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL AR

« Hide

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References

« Hide 'large scale' references
[1]"Nucleotide sequence of rat renin cDNA."
Tada M., Fukamizu A., Seo M.S., Takahashi S., Murakami K.
Nucleic Acids Res. 16:3576-3576(1988) [PubMed: 3287330] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[2]"Molecular cloning of rat renin cDNA and its gene."
Burnham C.E., Hawelu-Johnson C.L., Frank B.M., Lynch K.R.
Proc. Natl. Acad. Sci. U.S.A. 84:5605-5609(1987) [PubMed: 3039496] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structure of the rat renin gene."
Fukamizu A., Nishi K., Cho T., Saitoh M., Nakayama K., Ohkubo H., Nakanishi S., Murakami K.
J. Mol. Biol. 201:443-450(1988) [PubMed: 3047403] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Sprague-Dawley.
[4]"Renin gene nucleotide sequence of coding and regulatory regions in Dahl rats."
Alam K.Y., Wang Y., Dene H., Rapp J.P.
Clin. Exp. Hypertens. 15:599-614(1993) [PubMed: 8490598] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

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Cross-references

Sequence databases

X07033 mRNA. Translation: CAA30082.1.
J02941 mRNA. Translation: AAA42030.1.
M37278 Genomic DNA. Translation: AAA42031.1. Sequence problems.
S60054 mRNA. Translation: AAP13916.1.
BC078878 mRNA. Translation: AAH78878.1.
IPIIPI00211210.
PIRRERTK. A29991.
RefSeqNP_036774.4.
UniGeneRn.9831

3D structure databases

HSSPHSSP built from PDB template 1SMR based on UniProtKB P00796.
ModBaseSearch...

Protein family/group databases

MEROPSA01.007.

Genome annotation databases

EnsemblENSRNOG00000002937. Rattus norvegicus. [Contig view]
GeneID24715.
KEGGrno:24715.

Organism-specific databases

RGD3554. Ren1.

Phylogenomic databases

HOVERGENP08424.
OMAP08424. DPQHYQG.

Enzyme and pathway databases

BRENDA3.4.23.15. 248.

Gene expression databases

ArrayExpressP08424.
GermOnlineENSRNOG00000002937. Rattus norvegicus.

Family and domain databases

InterProIPR001461. Peptidase_A1.
IPR001969. Peptidase_aspartic_AS.
IPR009007. Peptidase_aspartic_catalytic.
IPR012848. Propep_A1.
[Graphical view]
Gene3DG3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHERPTHR13683. Peptidase_A1. 1 hit.
PfamPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSPR00792. PEPSIN.
PROSITEPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio604209.

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Entry information

Entry nameRENI_RAT
AccessionPrimary (citable) accession number: P08424
Secondary accession number(s): Q63497
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1996
Last modified: June 16, 2009
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents