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Protein

Renin

Gene

Ren1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activityi

Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Enzyme regulationi

Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102PROSITE-ProRule annotation1
Active sitei287PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: GO_Central
  • endopeptidase activity Source: RGD
  • insulin-like growth factor receptor binding Source: RGD
  • receptor binding Source: RGD

GO - Biological processi

  • angiotensin maturation Source: RGD
  • beta-amyloid metabolic process Source: RGD
  • cell maturation Source: Ensembl
  • cellular response to drug Source: RGD
  • drinking behavior Source: Ensembl
  • hormone-mediated signaling pathway Source: Ensembl
  • male gonad development Source: Ensembl
  • mesonephros development Source: Ensembl
  • regulation of blood pressure Source: RGD
  • regulation of MAPK cascade Source: Ensembl
  • renin-angiotensin regulation of aldosterone production Source: Ensembl
  • response to cAMP Source: RGD
  • response to cGMP Source: RGD
  • response to drug Source: RGD
  • response to immobilization stress Source: RGD
  • response to lipopolysaccharide Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-RNO-2022377. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiA01.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Renin (EC:3.4.23.15)
Alternative name(s):
Angiotensinogenase
Gene namesi
Name:Ren1
Synonyms:Ren
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi3554. Ren1.

Subcellular locationi

  • Secreted By similarity
  • Membrane By similarity

  • Note: Associated to membranes via binding to ATP6AP2.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: RGD
  • lysosome Source: GO_Central
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2322.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26CuratedAdd BLAST26
PropeptideiPRO_000002609127 – 64Activation peptideAdd BLAST38
ChainiPRO_000002609265 – 402ReninAdd BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi69N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi115 ↔ 122By similarity
Glycosylationi139N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi278 ↔ 282By similarity
Glycosylationi320N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP08424.

PTM databases

PhosphoSitePlusiP08424.

Expressioni

Gene expression databases

BgeeiENSRNOG00000002937.
ExpressionAtlasiP08424. baseline and differential.
GenevisibleiP08424. RN.

Interactioni

Subunit structurei

Interacts with ATP6AP2.By similarity

GO - Molecular functioni

  • insulin-like growth factor receptor binding Source: RGD
  • receptor binding Source: RGD

Protein-protein interaction databases

BioGridi246843. 1 interactor.
STRINGi10116.ENSRNOP00000003951.

Chemistry databases

BindingDBiP08424.

Structurei

3D structure databases

ProteinModelPortaliP08424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini84 – 399Peptidase A1PROSITE-ProRule annotationAdd BLAST316

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP08424.
KOiK01380.
OMAiNFIARYY.
OrthoDBiEOG091G0JP7.
PhylomeDBiP08424.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGRRMPLWA LLLLWTSCSF SLPTDTASFG RILLKKMPSV REILEERGVD
60 70 80 90 100
MTRISAEWGE FIKKSSFTNV TSPVVLTNYL DTQYYGEIGI GTPSQTFKVI
110 120 130 140 150
FDTGSANLWV PSTKCGPLYT ACEIHNLYDS SESSSYMENG TEFTIHYGSG
160 170 180 190 200
KVKGFLSQDV VTVGGIIVTQ TFGEVTELPL IPFMLAKFDG VLGMGFPAQA
210 220 230 240 250
VDGVIPVFDH ILSQRVLKEE VFSVYYSRES HLLGGEVVLG GSDPQHYQGN
260 270 280 290 300
FHYVSISKAG SWQITMKGVS VGPATLLCEE GCMAVVDTGT SYISGPTSSL
310 320 330 340 350
QLIMQALGVK EKRANNYVVN CSQVPTLPDI SFYLGGRTYT LSNMDYVQKN
360 370 380 390 400
PFRNDDLCIL ALQGLDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL

AR
Length:402
Mass (Da):44,276
Last modified:February 1, 1996 - v2
Checksum:iE4E22CEE449EAC3F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti200A → V in CAA30082 (PubMed:3287330).Curated1
Sequence conflicti214 – 215QR → HE in AAA42030 (PubMed:3039496).Curated2
Sequence conflicti271V → L in CAA30082 (PubMed:3287330).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07033 mRNA. Translation: CAA30082.1.
J02941 mRNA. Translation: AAA42030.1.
M37278 Genomic DNA. Translation: AAA42031.1. Sequence problems.
S60054 mRNA. Translation: AAP13916.1.
BC078878 mRNA. Translation: AAH78878.1.
PIRiA29991. RERTK.
RefSeqiNP_036774.4. NM_012642.4.
UniGeneiRn.9831.

Genome annotation databases

EnsembliENSRNOT00000003951; ENSRNOP00000003951; ENSRNOG00000002937.
GeneIDi24715.
KEGGirno:24715.
UCSCiRGD:3554. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07033 mRNA. Translation: CAA30082.1.
J02941 mRNA. Translation: AAA42030.1.
M37278 Genomic DNA. Translation: AAA42031.1. Sequence problems.
S60054 mRNA. Translation: AAP13916.1.
BC078878 mRNA. Translation: AAH78878.1.
PIRiA29991. RERTK.
RefSeqiNP_036774.4. NM_012642.4.
UniGeneiRn.9831.

3D structure databases

ProteinModelPortaliP08424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246843. 1 interactor.
STRINGi10116.ENSRNOP00000003951.

Chemistry databases

BindingDBiP08424.
ChEMBLiCHEMBL2322.

Protein family/group databases

MEROPSiA01.007.

PTM databases

PhosphoSitePlusiP08424.

Proteomic databases

PaxDbiP08424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003951; ENSRNOP00000003951; ENSRNOG00000002937.
GeneIDi24715.
KEGGirno:24715.
UCSCiRGD:3554. rat.

Organism-specific databases

CTDi5972.
RGDi3554. Ren1.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP08424.
KOiK01380.
OMAiNFIARYY.
OrthoDBiEOG091G0JP7.
PhylomeDBiP08424.
TreeFamiTF314990.

Enzyme and pathway databases

ReactomeiR-RNO-2022377. Metabolism of Angiotensinogen to Angiotensins.

Miscellaneous databases

PROiP08424.

Gene expression databases

BgeeiENSRNOG00000002937.
ExpressionAtlasiP08424. baseline and differential.
GenevisibleiP08424. RN.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRENI_RAT
AccessioniPrimary (citable) accession number: P08424
Secondary accession number(s): Q63497
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.