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P08424 (RENI_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Renin

EC=3.4.23.15
Alternative name(s):
Angiotensinogenase
Gene names
Name:Ren1
Synonyms:Ren
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length402 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activity

Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Enzyme regulation

Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing By similarity.

Subunit structure

Interacts with ATP6AP2 By similarity.

Subcellular location

Secreted By similarity. Membrane By similarity. Note: Associated to membranes via binding to ATP6AP2 By similarity.

Sequence similarities

Belongs to the peptidase A1 family.

Ontologies

Keywords
   Cellular componentMembrane
Secreted
   DomainSignal
   Molecular functionAspartyl protease
Hydrolase
Protease
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiotensin maturation

Inferred from direct assay PubMed 21321306. Source: RGD

beta-amyloid metabolic process

Inferred from expression pattern PubMed 22609375. Source: RGD

cell maturation

Inferred from electronic annotation. Source: Ensembl

cellular response to drug

Inferred from expression pattern PubMed 21963836. Source: RGD

drinking behavior

Inferred from electronic annotation. Source: Ensembl

hormone-mediated signaling pathway

Inferred from electronic annotation. Source: Ensembl

male gonad development

Inferred from electronic annotation. Source: Ensembl

mesonephros development

Inferred from electronic annotation. Source: Ensembl

regulation of MAPK cascade

Inferred from electronic annotation. Source: Ensembl

regulation of blood pressure

Inferred from mutant phenotype PubMed 8567976. Source: RGD

renin-angiotensin regulation of aldosterone production

Inferred from electronic annotation. Source: Ensembl

response to cAMP

Inferred from direct assay PubMed 9671794. Source: RGD

response to cGMP

Inferred from direct assay PubMed 9671794. Source: RGD

response to drug

Inferred from direct assay PubMed 9671794. Source: RGD

response to immobilization stress

Inferred from expression pattern PubMed 7042704. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern PubMed 22342485. Source: RGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 12242043. Source: RGD

extracellular space

Inferred from direct assay PubMed 1278112. Source: RGD

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaspartic-type endopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

endopeptidase activity

Inferred from direct assay PubMed 7060565. Source: RGD

insulin-like growth factor receptor binding

Inferred from direct assay PubMed 11247783. Source: RGD

receptor binding

Inferred from physical interaction PubMed 21321306. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Probable
Propeptide27 – 6438Activation peptide
PRO_0000026091
Chain65 – 402338Renin
PRO_0000026092

Sites

Active site1021 By similarity
Active site2871 By similarity

Amino acid modifications

Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation1391N-linked (GlcNAc...) Potential
Glycosylation3201N-linked (GlcNAc...) Potential
Disulfide bond115 ↔ 122 By similarity
Disulfide bond278 ↔ 282 By similarity

Experimental info

Sequence conflict2001A → V in CAA30082. Ref.1
Sequence conflict214 – 2152QR → HE in AAA42030. Ref.2
Sequence conflict2711V → L in CAA30082. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P08424 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E4E22CEE449EAC3F

FASTA40244,276
        10         20         30         40         50         60 
MGGRRMPLWA LLLLWTSCSF SLPTDTASFG RILLKKMPSV REILEERGVD MTRISAEWGE 

        70         80         90        100        110        120 
FIKKSSFTNV TSPVVLTNYL DTQYYGEIGI GTPSQTFKVI FDTGSANLWV PSTKCGPLYT 

       130        140        150        160        170        180 
ACEIHNLYDS SESSSYMENG TEFTIHYGSG KVKGFLSQDV VTVGGIIVTQ TFGEVTELPL 

       190        200        210        220        230        240 
IPFMLAKFDG VLGMGFPAQA VDGVIPVFDH ILSQRVLKEE VFSVYYSRES HLLGGEVVLG 

       250        260        270        280        290        300 
GSDPQHYQGN FHYVSISKAG SWQITMKGVS VGPATLLCEE GCMAVVDTGT SYISGPTSSL 

       310        320        330        340        350        360 
QLIMQALGVK EKRANNYVVN CSQVPTLPDI SFYLGGRTYT LSNMDYVQKN PFRNDDLCIL 

       370        380        390        400 
ALQGLDIPPP TGPVWVLGAT FIRKFYTEFD RHNNRIGFAL AR 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of rat renin cDNA."
Tada M., Fukamizu A., Seo M.S., Takahashi S., Murakami K.
Nucleic Acids Res. 16:3576-3576(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[2]"Molecular cloning of rat renin cDNA and its gene."
Burnham C.E., Hawelu-Johnson C.L., Frank B.M., Lynch K.R.
Proc. Natl. Acad. Sci. U.S.A. 84:5605-5609(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structure of the rat renin gene."
Fukamizu A., Nishi K., Cho T., Saitoh M., Nakayama K., Ohkubo H., Nakanishi S., Murakami K.
J. Mol. Biol. 201:443-450(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Sprague-Dawley.
[4]"Renin gene nucleotide sequence of coding and regulatory regions in Dahl rats."
Alam K.Y., Wang Y., Dene H., Rapp J.P.
Clin. Exp. Hypertens. 15:599-614(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X07033 mRNA. Translation: CAA30082.1.
J02941 mRNA. Translation: AAA42030.1.
M37278 Genomic DNA. Translation: AAA42031.1. Sequence problems.
S60054 mRNA. Translation: AAP13916.1.
BC078878 mRNA. Translation: AAH78878.1.
PIRRERTK. A29991.
RefSeqNP_036774.4. NM_012642.4.
UniGeneRn.9831.

3D structure databases

ProteinModelPortalP08424.
SMRP08424. Positions 68-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246843. 1 interaction.

Chemistry

BindingDBP08424.
ChEMBLCHEMBL2322.

Protein family/group databases

MEROPSA01.007.

PTM databases

PhosphoSiteP08424.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000003951; ENSRNOP00000003951; ENSRNOG00000002937.
GeneID24715.
KEGGrno:24715.
UCSCRGD:3554. rat.

Organism-specific databases

CTD5972.
RGD3554. Ren1.

Phylogenomic databases

eggNOGNOG248684.
GeneTreeENSGT00710000106265.
HOGENOMHOG000197681.
HOVERGENHBG000482.
InParanoidP08424.
KOK01380.
OMAGSDPAYY.
OrthoDBEOG7HQN88.
PhylomeDBP08424.
TreeFamTF314990.

Gene expression databases

GenevestigatorP08424.

Family and domain databases

Gene3D2.40.70.10. 2 hits.
InterProIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERPTHR13683. PTHR13683. 1 hit.
PfamPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSPR00792. PEPSIN.
SUPFAMSSF50630. SSF50630. 1 hit.
PROSITEPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio604209.
PROP08424.

Entry information

Entry nameRENI_RAT
AccessionPrimary (citable) accession number: P08424
Secondary accession number(s): Q63497
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries