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Protein

Chymotrypsin-like elastase family member 2A

Gene

CELA2A

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts upon elastin.

Catalytic activityi

Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Charge relay system
Active sitei121 – 1211Charge relay system
Active sitei216 – 2161Charge relay system

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.155.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-like elastase family member 2A (EC:3.4.21.71)
Alternative name(s):
Elastase-2
Elastase-2A
Gene namesi
Name:CELA2A
Synonyms:ELA2, ELA2A
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2096984.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Add
BLAST
Propeptidei17 – 2812Activation peptidePRO_0000027689Add
BLAST
Chaini29 – 269241Chymotrypsin-like elastase family member 2APRO_0000027690Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi58 ↔ 74PROSITE-ProRule annotation1 Publication
Disulfide bondi155 ↔ 222PROSITE-ProRule annotation1 Publication
Disulfide bondi186 ↔ 202PROSITE-ProRule annotation1 Publication
Disulfide bondi212 ↔ 243PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP08419.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

GenevisibleiP08419. SS.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000003756.

Chemistry

BindingDBiP08419.

Structurei

Secondary structure

1
269
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi43 – 508Combined sources
Beta strandi53 – 6412Combined sources
Beta strandi67 – 704Combined sources
Helixi72 – 743Combined sources
Beta strandi81 – 866Combined sources
Beta strandi88 – 925Combined sources
Beta strandi98 – 10710Combined sources
Helixi116 – 1183Combined sources
Beta strandi123 – 1297Combined sources
Beta strandi154 – 1607Combined sources
Beta strandi174 – 1818Combined sources
Helixi183 – 1864Combined sources
Turni189 – 1924Combined sources
Helixi193 – 1953Combined sources
Beta strandi200 – 2034Combined sources
Beta strandi206 – 2105Combined sources
Beta strandi219 – 2235Combined sources
Beta strandi229 – 2379Combined sources
Beta strandi242 – 2443Combined sources
Beta strandi250 – 2545Combined sources
Helixi255 – 2573Combined sources
Helixi259 – 26810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRUX-ray2.30P29-269[»]
ProteinModelPortaliP08419.
SMRiP08419. Positions 29-269.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08419.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 267239Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
InParanoidiP08419.
KOiK01346.
OMAiAWWGSSV.
OrthoDBiEOG75B84T.
TreeFamiTF330455.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08419-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRALLLSTL VAGALSCGLP ANLPQLPRVV GGEDARPNSW PWQVSLQYDS
60 70 80 90 100
SGQWRHTCGG TLVDQSWVLT AAHCISSSRT YRVVLGRHSL STNEPGSLAV
110 120 130 140 150
KVSKLVVHQD WNSNQLSNGN DIALLKLASP VSLTDKIQLG CLPAAGTILP
160 170 180 190 200
NNYVCYVTGW GRLQTNGASP DILQQGQLLV VDYATCSKPG WWGSTVKTNM
210 220 230 240 250
ICAGGDGIIS SCNGDSGGPL NCQGANGQWQ VHGIVSFGSS LGCNYYHKPS
260
VFTRVSNYID WINSVIANN
Length:269
Mass (Da):28,699
Last modified:August 1, 1988 - v1
Checksum:iBAC6FE69AF4DDE56
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101L → S in BAA00166 (PubMed:2834346).Curated
Sequence conflicti118 – 1181N → K in BAA00166 (PubMed:2834346).Curated
Sequence conflicti132 – 1321S → Y in BAA00166 (PubMed:2834346).Curated
Sequence conflicti172 – 1721I → V in BAA00166 (PubMed:2834346).Curated
Sequence conflicti202 – 2021C → V in BAA00166 (PubMed:2834346).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16651 mRNA. Translation: AAA31027.1.
D00237 mRNA. Translation: BAA00166.1.
PIRiA26823.
RefSeqiNP_999274.1. NM_214109.1.
UniGeneiSsc.316.

Genome annotation databases

EnsembliENSSSCT00000003844; ENSSSCP00000003756; ENSSSCG00000003459.
GeneIDi397197.
KEGGissc:397197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16651 mRNA. Translation: AAA31027.1.
D00237 mRNA. Translation: BAA00166.1.
PIRiA26823.
RefSeqiNP_999274.1. NM_214109.1.
UniGeneiSsc.316.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRUX-ray2.30P29-269[»]
ProteinModelPortaliP08419.
SMRiP08419. Positions 29-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000003756.

Chemistry

BindingDBiP08419.
ChEMBLiCHEMBL2096984.

Protein family/group databases

MEROPSiS01.155.

Proteomic databases

PaxDbiP08419.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000003844; ENSSSCP00000003756; ENSSSCG00000003459.
GeneIDi397197.
KEGGissc:397197.

Organism-specific databases

CTDi63036.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
InParanoidiP08419.
KOiK01346.
OMAiAWWGSSV.
OrthoDBiEOG75B84T.
TreeFamiTF330455.

Miscellaneous databases

EvolutionaryTraceiP08419.
PROiP08419.

Gene expression databases

GenevisibleiP08419. SS.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of pancreatic elastase II cDNAs: two elastase II mRNAs are expressed in human pancreas."
    Kawashima I., Tani T., Shimoda K., Takiguchi Y.
    DNA 6:163-172(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and expression in Escherichia coli of a cDNA encoding human pancreatic elastase 2."
    Shirasu Y., Yoshida H., Matsuki S., Takemura K., Ikeda N., Shimada Y., Ozawa T., Mikayama T., Iijima H., Ishida A., Sato Y., Tamai Y., Tanaka J., Ikenaga H.
    J. Biochem. 102:1555-1563(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Structure of porcine pancreatic elastase complexed with the elastase inhibitor GR143783."
    Jhoti H., Singh O.M.P., Wonacott A.
    Submitted (AUG-1999) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 29-269 IN COMPLEX WITH INHIBITOR, DISULFIDE BONDS.

Entry informationi

Entry nameiCEL2A_PIG
AccessioniPrimary (citable) accession number: P08419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.