Reviewed, UniProtKB/Swiss-Prot P08413 (KCC2B_RAT)

Names and origin

Protein names

Recommended name:
    Calcium/calmodulin-dependent protein kinase type II subunit beta
      Short name=CaM kinase II subunit beta
      Short name=CaMK-II subunit beta
    EC=2.7.11.17

Gene names

Name:

Camk2b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Autophosphorylation of CAMK2 plays an important role in the regulation of the kinase activity.
Subunit structure	CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with SYNGAP1, CAMK2N2 and MPDZ. Ref.3 Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	calcium- and calmodulin-dependent protein kinase complex Traceable author statement. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	Calcium/calmodulin-dependent protein kinase type II subunit beta		PRO_0000086098
Regions
	Domain	14 – 272	259	Protein kinase
	Nucleotide binding	20 – 28	9	ATP By similarity
	Region	291 – 301	11	Calmodulin-binding
Sites
	Active site	136	1	Proton acceptor By similarity
	Binding site	43	1	ATP By similarity
Amino acid modifications
	Modified residue	17	1	Phosphotyrosine By similarity
	Modified residue	287	1	Phosphothreonine By similarity
	Modified residue	315	1	Phosphoserine By similarity
	Modified residue	358	1	Phosphoserine By similarity
	Modified residue	367	1	Phosphoserine By similarity
	Modified residue	397	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08413-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 8A7962A64AE930D0	FASTA	542	60,402
	10 20 30 40 50 60 MATTVTCTRF TDEYQLYEDI GKGAFSVVRR CVKLCTGHEY AAKIINTKKL SARDHQKLER 70 80 90 100 110 120 EARICRLLKH SNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI 130 140 150 160 170 180 LEAVLHCHQM GVVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY 190 200 210 220 230 240 LSPEVLRKEA YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT 250 260 270 280 290 300 VTPEAKNLIN QMLTINPAKR ITAHEALKHP WVCQRSTVAS MMHRQETVEC LKKFNARRKL 310 320 330 340 350 360 KGAILTTMLA TRNFSVGRQT TAPATMSTAA SGTTMGLVEQ AKSLLNKKAD GVKPQTNSTK 370 380 390 400 410 420 NSSAITSPKG SLPPAALEPQ TTVIHNPVDG IKESSDSTNT TIEDEDAKAR KQEIIKTTEQ 430 440 450 460 470 480 LIEAVNNGDF EAYAKICDPG LTSFEPEALG NLVEGMDFHR FYFENLLAKN SKPIHTTILN 490 500 510 520 530 540 PHVHVIGEDA ACIAYIRLTQ YIDGQGRPRT SQSEETRVWH RPDGKWQNVH FHCSGAPVAP LQ

References

[1]	"Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning." Bennett M.K., Kennedy M.B. Proc. Natl. Acad. Sci. U.S.A. 84:1794-1798(1987) [PubMed: 3470758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Characterization of a calmodulin kinase II inhibitor protein in brain." Chang B.H., Mukherji S., Soderling T.R. Proc. Natl. Acad. Sci. U.S.A. 95:10890-10895(1998) [PubMed: 9724800] [Abstract] Cited for: INTERACTION WITH CAMK2N2.
[3]	"SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation." Krapivinsky G., Medina I., Krapivinsky L., Gapon S., Clapham D.E. Neuron 43:563-574(2004) [PubMed: 15312654] [Abstract] Cited for: INTERACTION WITH SYNGAP1 AND MPDZ, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16112 mRNA. Translation: AAA41866.1.
IPI	IPI00211185.
PIR	A26464.
RefSeq	NP_001035815.1. NP_068507.2.
UniGene	Rn.9743
3D structure databases
SMR	P08413. Positions 11-301, 403-536.
ModBase	Search...
Protein-protein interaction databases
STRING	P08413.
PTM databases
PhosphoSite	P08413.
Genome annotation databases
Ensembl	ENSRNOT00000045935; ENSRNOP00000046543; ENSRNOG00000004005; Rattus norvegicus. [Genome view]
GeneID	24245.
UCSC	NM_021739. rat.
Organism-specific databases
CTD	24245.
RGD	2262. Camk2b.
Phylogenomic databases
eggNOG	maNOG17752.
HOVERGEN	P08413.
PhylomeDB	P08413.
Enzyme and pathway databases
BRENDA	2.7.11.17. 248.
Gene expression databases
ArrayExpress	P08413.
Genevestigator	P08413.
GermOnline	ENSRNOG00000004005. Rattus norvegicus.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR015742. Ca/CaM-dep_prot_kinase_2. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF64. CaMKII. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602737.

Entry information

Entry name

KCC2B_RAT

Accession

Primary (citable) accession number: P08413

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	February 9, 2010
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)