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Protein

Sensor protein CreC

Gene

creC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system CreC/CreB involved in catabolic regulation. CreC may function as a membrane-associated protein kinase that phosphorylates CreB in response to environmental signals. CreC can also phosphorylate PhoB.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:CREC-MONOMER.
ECOL316407:JW4362-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein CreC (EC:2.7.13.3)
Gene namesi
Name:creC
Synonyms:phoM
Ordered Locus Names:b4399, JW4362
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10730. creC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66PeriplasmicSequence analysis
Transmembranei7 – 2721HelicalSequence analysisAdd
BLAST
Topological domaini28 – 146119CytoplasmicSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Topological domaini168 – 18316PeriplasmicSequence analysisAdd
BLAST
Transmembranei184 – 20421HelicalSequence analysisAdd
BLAST
Topological domaini205 – 474270CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Sensor protein CreCPRO_0000074742Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei265 – 2651Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP08401.
PaxDbiP08401.
PRIDEiP08401.

Interactioni

Protein-protein interaction databases

BioGridi4261905. 10 interactions.
DIPiDIP-9319N.
IntActiP08401. 5 interactions.
MINTiMINT-1307740.
STRINGi511145.b4399.

Structurei

3D structure databases

ProteinModelPortaliP08401.
SMRiP08401. Positions 212-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini205 – 25551HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini262 – 473212Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EB3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000211121.
InParanoidiP08401.
KOiK07641.
OMAiIRWNNEI.
OrthoDBiEOG6G4VQG.
PhylomeDBiP08401.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGMRLLLG YFLLVAVAAW FVLAIFVKEV KPGVRRATEG TLIDTATLLA
60 70 80 90 100
ELARPDLLSG DPTHGQLAQA FNQLQHRPFR ANIGGINKVR NEYHVYMTDA
110 120 130 140 150
QGKVLFDSAN KAVGQDYSRW NDVWLTLRGQ YGARSTLQNP ADPESSVMYV
160 170 180 190 200
AAPIMDGSRL IGVLSVGKPN AAMAPVIKRS ERRILWASAI LLGIALVIGA
210 220 230 240 250
GMVWWINRSI ARLTRYADSV TDNKPVPLPD LGSSELRKLA QALESMRVKL
260 270 280 290 300
EGKNYIEQYV YALTHELKSP LAAIRGAAEI LREGPPPEVV ARFTDNILTQ
310 320 330 340 350
NARMQALVET LLRQARLENR QEVVLTAVDV AALFRRVSEA RTVQLAEKKI
360 370 380 390 400
TLHVTPTEVN VAAEPALLEQ ALGNLLDNAI DFTPESGCIT LSAEVDQEHV
410 420 430 440 450
TLKVLDTGSG IPDYALSRIF ERFYSLPRAN GQKSSGLGLA FVSEVARLFN
460 470
GEVTLRNVQE GGVLASLRLH RHFT
Length:474
Mass (Da):52,176
Last modified:July 19, 2003 - v2
Checksum:i9CEB8CCE406E3163
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771R → P in AAA24375 (PubMed:3531171).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13608 Genomic DNA. Translation: AAA24375.1.
U14003 Genomic DNA. Translation: AAA97295.1.
U00096 Genomic DNA. Translation: AAC77352.1.
AP009048 Genomic DNA. Translation: BAE78388.1.
Y00538 Genomic DNA. Translation: CAA68601.1.
PIRiS56623. RGECFM.
RefSeqiNP_418816.1. NC_000913.3.
WP_001219559.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77352; AAC77352; b4399.
BAE78388; BAE78388; BAE78388.
GeneIDi948609.
KEGGiecj:JW4362.
eco:b4399.
PATRICi32124416. VBIEscCol129921_4548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13608 Genomic DNA. Translation: AAA24375.1.
U14003 Genomic DNA. Translation: AAA97295.1.
U00096 Genomic DNA. Translation: AAC77352.1.
AP009048 Genomic DNA. Translation: BAE78388.1.
Y00538 Genomic DNA. Translation: CAA68601.1.
PIRiS56623. RGECFM.
RefSeqiNP_418816.1. NC_000913.3.
WP_001219559.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP08401.
SMRiP08401. Positions 212-469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261905. 10 interactions.
DIPiDIP-9319N.
IntActiP08401. 5 interactions.
MINTiMINT-1307740.
STRINGi511145.b4399.

Proteomic databases

EPDiP08401.
PaxDbiP08401.
PRIDEiP08401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77352; AAC77352; b4399.
BAE78388; BAE78388; BAE78388.
GeneIDi948609.
KEGGiecj:JW4362.
eco:b4399.
PATRICi32124416. VBIEscCol129921_4548.

Organism-specific databases

EchoBASEiEB0723.
EcoGeneiEG10730. creC.

Phylogenomic databases

eggNOGiENOG4105EB3. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000211121.
InParanoidiP08401.
KOiK07641.
OMAiIRWNNEI.
OrthoDBiEOG6G4VQG.
PhylomeDBiP08401.

Enzyme and pathway databases

BioCyciEcoCyc:CREC-MONOMER.
ECOL316407:JW4362-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP08401.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon."
    Amemura M., Makino K., Shinagawa H., Nakata A.
    J. Bacteriol. 168:294-302(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification and sequencing of the Escherichia coli cet gene which codes for an inner membrane protein, mutation of which causes tolerance to colicin E2."
    Drury L.S., Buxton R.S.
    Mol. Microbiol. 2:109-119(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 381-474.
    Strain: K12.
  6. "Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2."
    Amemura M., Makino K., Shinagawa H., Nakata A.
    J. Bacteriol. 172:6300-6307(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
    Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
    J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, AUTOPHOSPHORYLATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCREC_ECOLI
AccessioniPrimary (citable) accession number: P08401
Secondary accession number(s): Q2M5R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 19, 2003
Last modified: July 6, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.