P08401 (CREC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sensor protein CreC
EC=2.7.13.3

Gene names

Name:	creC
Synonyms:	phoM
Ordered Locus Names:	b4399, JW4362

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system CreC/CreB involved in catabolic regulation. CreC may function as a membrane-associated protein kinase that phosphorylates CreB in response to environmental signals. CreC can also phosphorylate PhoB. Ref.6 Ref.7
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Post-translational modification	Autophosphorylated. Ref.7
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from direct assay Ref.6. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from direct assay Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Sensor protein CreC		PRO_0000074742
Regions
	Topological domain	1 – 6	6	Periplasmic Potential
	Transmembrane	7 – 27	21	Helical; Potential
	Topological domain	28 – 146	119	Cytoplasmic Potential
	Transmembrane	147 – 167	21	Helical; Potential
	Topological domain	168 – 183	16	Periplasmic Potential
	Transmembrane	184 – 204	21	Helical; Potential
	Topological domain	205 – 474	270	Cytoplasmic Potential
	Domain	205 – 255	51	HAMP
	Domain	262 – 473	212	Histidine kinase
Amino acid modifications
	Modified residue	265	1	Phosphohistidine; by autocatalysis By similarity
Experimental info
	Sequence conflict	77	1	R → P in AAA24375. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P08401 [UniParc]. Last modified July 19, 2003. Version 2. Checksum: 9CEB8CCE406E3163	FASTA	474	52,176
	10 20 30 40 50 60 MRIGMRLLLG YFLLVAVAAW FVLAIFVKEV KPGVRRATEG TLIDTATLLA ELARPDLLSG 70 80 90 100 110 120 DPTHGQLAQA FNQLQHRPFR ANIGGINKVR NEYHVYMTDA QGKVLFDSAN KAVGQDYSRW 130 140 150 160 170 180 NDVWLTLRGQ YGARSTLQNP ADPESSVMYV AAPIMDGSRL IGVLSVGKPN AAMAPVIKRS 190 200 210 220 230 240 ERRILWASAI LLGIALVIGA GMVWWINRSI ARLTRYADSV TDNKPVPLPD LGSSELRKLA 250 260 270 280 290 300 QALESMRVKL EGKNYIEQYV YALTHELKSP LAAIRGAAEI LREGPPPEVV ARFTDNILTQ 310 320 330 340 350 360 NARMQALVET LLRQARLENR QEVVLTAVDV AALFRRVSEA RTVQLAEKKI TLHVTPTEVN 370 380 390 400 410 420 VAAEPALLEQ ALGNLLDNAI DFTPESGCIT LSAEVDQEHV TLKVLDTGSG IPDYALSRIF 430 440 450 460 470 ERFYSLPRAN GQKSSGLGLA FVSEVARLFN GEVTLRNVQE GGVLASLRLH RHFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon." Amemura M., Makino K., Shinagawa H., Nakata A. J. Bacteriol. 168:294-302(1986) [PubMed: 3531171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Identification and sequencing of the Escherichia coli cet gene which codes for an inner membrane protein, mutation of which causes tolerance to colicin E2." Drury L.S., Buxton R.S. Mol. Microbiol. 2:109-119(1988) [PubMed: 2835585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 381-474. Strain: K12.
[6]	"Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2." Amemura M., Makino K., Shinagawa H., Nakata A. J. Bacteriol. 172:6300-6307(1990) [PubMed: 2228961] [Abstract] Cited for: FUNCTION.
[7]	"Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A. J. Biol. Chem. 280:1448-1456(2005) [PubMed: 15522865] [Abstract] Cited for: FUNCTION, AUTOPHOSPHORYLATION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13608 Genomic DNA. Translation: AAA24375.1. U14003 Genomic DNA. Translation: AAA97295.1. U00096 Genomic DNA. Translation: AAC77352.1. AP009048 Genomic DNA. Translation: BAE78388.1. Y00538 Genomic DNA. Translation: CAA68601.1.
PIR	RGECFM. S56623.
RefSeq	NP_418816.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P08401.
SMR	P08401. Positions 204-472.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9319N.
IntAct	P08401. 4 interactions.
MINT	MINT-1307740.
Proteomic databases
PRIDE	P08401.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003217; EBESCP00000003217; EBESCG00000002642. EBESCT00000014937; EBESCP00000014228; EBESCG00000013997.
GeneID	948609.
GenomeReviews	Gene locus JW4362 in contig AP009048_GR. Gene locus b4399 in contig U00096_GR.
KEGG	ecj:JW4362. eco:b4399.
PATRIC	32124416. VBIEscCol129921_4548.
Organism-specific databases
EchoBASE	EB0723.
EcoGene	EG10730. creC.
Phylogenomic databases
eggNOG	COG0642.
GeneTree	EBGT00050000008662.
HOGENOM	HBG530303.
OMA	MPTEINV.
PhylomeDB	P08401.
ProtClustDB	PRK11100.
Enzyme and pathway databases
BioCyc	EcoCyc:CREC-MONOMER.
BRENDA	2.7.13.3. 2026.
Gene expression databases
Genevestigator	P08401.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
KO	K07641.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CREC_ECOLI

Accession

Primary (citable) accession number: P08401
Secondary accession number(s): Q2M5R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	July 19, 2003
Last modified:	January 25, 2012
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program