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Protein

Phosphate regulon sensor protein PhoR

Gene

phoR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system PhoR/PhoB involved in the phosphate regulon genes expression. PhoR may function as a membrane-associated protein kinase that phosphorylates PhoB in response to environmental signals.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to phosphate starvation Source: EcoCyc
  • phosphate ion transport Source: UniProtKB-KW
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphate transport, Transport, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PHOR-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate regulon sensor protein PhoR (EC:2.7.13.3)
Gene namesi
Name:phoR
Synonyms:nmpB
Ordered Locus Names:b0400, JW0390
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10733. phoR.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicCurated9
Transmembranei10 – 28HelicalCuratedAdd BLAST19
Topological domaini29 – 32PeriplasmicCurated4
Transmembranei33 – 51HelicalCuratedAdd BLAST19
Topological domaini52 – 431CytoplasmicCuratedAdd BLAST380

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000748461 – 431Phosphate regulon sensor protein PhoRAdd BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei213Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP08400.
PRIDEiP08400.

Interactioni

Protein-protein interaction databases

BioGridi4262091. 11 interactors.
DIPiDIP-10502N.
IntActiP08400. 4 interactors.
MINTiMINT-1288861.
STRINGi316385.ECDH10B_0356.

Structurei

3D structure databases

ProteinModelPortaliP08400.
SMRiP08400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 172PASPROSITE-ProRule annotationAdd BLAST77
Domaini210 – 425Histidine kinasePROSITE-ProRule annotationAdd BLAST216

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000223176.
InParanoidiP08400.
KOiK07636.
PhylomeDBiP08400.

Family and domain databases

CDDicd00130. PAS. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR021766. DUF3329.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR014310. Sig_transdc_His_kinase_PhoR.
PfamiView protein in Pfam
PF11808. DUF3329. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR02966. phoR_proteo. 1 hit.
PROSITEiView protein in PROSITE
PS50109. HIS_KIN. 1 hit.
PS50112. PAS. 1 hit.

Sequencei

Sequence statusi: Complete.

P08400-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLERLSWKRL VLELLLCCLP AFILGAFFGY LPWFLLASVT GLLIWHFWNL
60 70 80 90 100
LRLSWWLWVD RSMTPPPGRG SWEPLLYGLH QMQLRNKKRR RELGNLIKRF
110 120 130 140 150
RSGAESLPDA VVLTTEEGGI FWCNGLAQQI LGLRWPEDNG QNILNLLRYP
160 170 180 190 200
EFTQYLKTRD FSRPLNLVLN TGRHLEIRVM PYTHKQLLMV ARDVTQMHQL
210 220 230 240 250
EGARRNFFAN VSHELRTPLT VLQGYLEMMN EQPLEGAVRE KALHTMREQT
260 270 280 290 300
QRMEGLVKQL LTLSKIEAAP THLLNEKVDV PMMLRVVERE AQTLSQKKQT
310 320 330 340 350
FTFEIDNGLK VSGNEDQLRS AISNLVYNAV NHTPEGTHIT VRWQRVPHGA
360 370 380 390 400
EFSVEDNGPG IAPEHIPRLT ERFYRVDKAR SRQTGGSGLG LAIVKHAVNH
410 420 430
HESRLNIEST VGKGTRFSFV IPERLIAKNS D
Length:431
Mass (Da):49,629
Last modified:August 1, 1988 - v1
Checksum:i33883582AF4B883C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04704 Genomic DNA. Translation: CAA28409.1.
U73857 Genomic DNA. Translation: AAB18124.1.
U00096 Genomic DNA. Translation: AAC73503.1.
AP009048 Genomic DNA. Translation: BAE76180.1.
PIRiA25557. RGECFR.
RefSeqiNP_414934.1. NC_000913.3.
WP_000893623.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73503; AAC73503; b0400.
BAE76180; BAE76180; BAE76180.
GeneIDi945044.
KEGGiecj:JW0390.
eco:b0400.
PATRICifig|1411691.4.peg.1878.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPHOR_ECOLI
AccessioniPrimary (citable) accession number: P08400
Secondary accession number(s): Q2MC26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 5, 2017
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene