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Protein

Protease 4

Gene

sppA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Digests cleaved signal peptides in vitro, its in vivo function is unknown. This activity is necessary to maintain proper secretion of mature proteins across the membrane.4 Publications

Enzyme regulationi

Inhibited by serine hydrolase inhibitor FP-biotin and by antipain.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei209Proton donor/acceptorCurated1
Active sitei409Nucleophile1

GO - Molecular functioni

  • endopeptidase activity Source: EcoCyc
  • serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  • signal peptide processing Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10968-MONOMER.
ECOL316407:JW1755-MONOMER.
MetaCyc:EG10968-MONOMER.

Protein family/group databases

MEROPSiS49.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease 4 (EC:3.4.21.-)
Alternative name(s):
Endopeptidase IV
Protease IV
Signal peptide peptidase
Gene namesi
Name:sppA
Ordered Locus Names:b1766, JW1755
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10968. sppA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicAdd BLAST24
Transmembranei25 – 45HelicalAdd BLAST21
Topological domaini46 – 618PeriplasmicAdd BLAST573

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No effect on processing of liberated signal peptides in vivo.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi209K → A: Loss of activity. 1 Publication1
Mutagenesisi366K → A: Reduced activity. 1 Publication1
Mutagenesisi409S → A: Loss of activity. 1 Publication1
Mutagenesisi410G → A: Reduced activity. 1 Publication1
Mutagenesisi412Y → F: No effect on activity. 1 Publication1
Mutagenesisi510H → A: Reduced activity. 1 Publication1
Mutagenesisi524D → N: Increased activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714381 – 618Protease 4Add BLAST618

Proteomic databases

EPDiP08395.
PaxDbiP08395.
PRIDEiP08395.

Interactioni

Subunit structurei

Homotetramer.2 Publications

Protein-protein interaction databases

BioGridi4259140. 4 interactors.
IntActiP08395. 1 interactor.
STRINGi511145.b1766.

Structurei

Secondary structure

1618
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi58 – 61Combined sources4
Beta strandi64 – 69Combined sources6
Beta strandi94 – 96Combined sources3
Helixi97 – 109Combined sources13
Beta strandi116 – 119Combined sources4
Beta strandi122 – 125Combined sources4
Helixi128 – 143Combined sources16
Beta strandi148 – 153Combined sources6
Helixi157 – 163Combined sources7
Beta strandi166 – 171Combined sources6
Beta strandi186 – 188Combined sources3
Helixi190 – 195Combined sources6
Beta strandi199 – 205Combined sources7
Helixi210 – 212Combined sources3
Helixi213 – 216Combined sources4
Helixi222 – 247Combined sources26
Helixi251 – 254Combined sources4
Helixi257 – 266Combined sources10
Turni267 – 270Combined sources4
Helixi272 – 278Combined sources7
Beta strandi281 – 285Combined sources5
Helixi288 – 299Combined sources12
Turni303 – 305Combined sources3
Beta strandi306 – 312Combined sources7
Turni313 – 315Combined sources3
Beta strandi327 – 342Combined sources16
Beta strandi346 – 348Combined sources3
Helixi349 – 361Combined sources13
Beta strandi365 – 377Combined sources13
Helixi379 – 394Combined sources16
Beta strandi399 – 408Combined sources10
Helixi410 – 413Combined sources4
Turni414 – 417Combined sources4
Beta strandi419 – 423Combined sources5
Beta strandi428 – 430Combined sources3
Beta strandi434 – 440Combined sources7
Helixi442 – 447Combined sources6
Beta strandi451 – 453Combined sources3
Helixi459 – 461Combined sources3
Helixi471 – 495Combined sources25
Helixi500 – 504Combined sources5
Beta strandi511 – 513Combined sources3
Helixi514 – 519Combined sources6
Beta strandi524 – 526Combined sources3
Helixi529 – 539Combined sources11
Beta strandi545 – 548Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BEZX-ray2.76A/B/C/D47-618[»]
3BF0X-ray2.55A/B/C/D47-618[»]
ProteinModelPortaliP08395.
SMRiP08395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08395.

Family & Domainsi

Domaini

A tandem duplication in the protein fold creates an intact active site between the repeated domains of each monomer.

Sequence similaritiesi

Belongs to the peptidase S49 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D07. Bacteria.
COG0616. LUCA.
InParanoidiP08395.
KOiK04773.
OMAiWSKADNN.
PhylomeDBiP08395.

Family and domain databases

CDDicd07019. S49_SppA_1. 1 hit.
Gene3Di3.90.226.10. 4 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR004634. Pept_S49_pIV.
IPR004635. Pept_S49_SppA.
IPR002142. Peptidase_S49.
IPR033854. S49_SppA_1.
[Graphical view]
PfamiPF01343. Peptidase_S49. 2 hits.
[Graphical view]
PIRSFiPIRSF001217. Protease_4_SppA. 1 hit.
SUPFAMiSSF52096. SSF52096. 4 hits.
TIGRFAMsiTIGR00705. SppA_67K. 1 hit.
TIGR00706. SppA_dom. 1 hit.

Sequencei

Sequence statusi: Complete.

P08395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLWRFIAG FFKWTWRLLN FVREMVLNLF FIFLVLVGVG IWMQVSGGDS
60 70 80 90 100
KETASRGALL LDISGVIVDK PDSSQRFSKL SRQLLGASSD RLQENSLFDI
110 120 130 140 150
VNTIRQAKDD RNITGIVMDL KNFAGGDQPS MQYIGKALKE FRDSGKPVYA
160 170 180 190 200
VGENYSQGQY YLASFANKIW LSPQGVVDLH GFATNGLYYK SLLDKLKVST
210 220 230 240 250
HVFRVGTYKS AVEPFIRDDM SPAAREADSR WIGELWQNYL NTVAANRQIP
260 270 280 290 300
AEQVFPGAQG LLEGLTKTGG DTAKYALENK LVDALASSAE IEKALTKEFG
310 320 330 340 350
WSKTDKNYRA ISYYDYALKT PADTGDSIGV VFANGAIMDG EETQGNVGGD
360 370 380 390 400
TTAAQIRDAR LDPKVKAIVL RVNSPGGSVT ASEVIRAELA AARAAGKPVV
410 420 430 440 450
VSMGGMAASG GYWISTPANY IVANPSTLTG SIGIFGVITT VENSLDSIGV
460 470 480 490 500
HTDGVSTSPL ADVSITRALP PEAQLMMQLS IENGYKRFIT LVADARHSTP
510 520 530 540 550
EQIDKIAQGH VWTGQDAKAN GLVDSLGDFD DAVAKAAELA KVKQWHLEYY
560 570 580 590 600
VDEPTFFDKV MDNMSGSVRA MLPDAFQAML PAPLASVAST VKSESDKLAA
610
FNDPQNRYAF CLTCANMR
Length:618
Mass (Da):67,219
Last modified:August 29, 2003 - v2
Checksum:iEAF00496B5946AC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti378S → T in AAA24648 (PubMed:3522590).Curated1
Sequence conflicti378S → T in AAA57009 (PubMed:7973728).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti151V → I in strain: ECOR 49 and ECOR 50. 1
Natural varianti186G → S in strain: ECOR 16. 1
Natural varianti252E → H in strain: ECOR 16. 1
Natural varianti252E → K in strain: ECOR 49. 1
Natural varianti252E → Q in strain: ECOR 38, ECOR 39, ECOR 40, ECOR 50, ECOR 65 and ECOR 68. 1
Natural varianti294A → T in strain: ECOR 38, ECOR 39, ECOR 40, ECOR 49, ECOR 50 and ECOR 65. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13359 Genomic DNA. Translation: AAA24648.1.
U00096 Genomic DNA. Translation: AAC74836.1.
AP009048 Genomic DNA. Translation: BAA15557.1.
U13772 Genomic DNA. Translation: AAA57008.1.
U13773 Genomic DNA. Translation: AAA57009.1.
U13774 Genomic DNA. Translation: AAA57010.1.
U13775 Genomic DNA. Translation: AAA57011.1.
U13776 Genomic DNA. Translation: AAA57012.1.
U13777 Genomic DNA. Translation: AAA57013.1.
U13778 Genomic DNA. Translation: AAA57014.1.
U13779 Genomic DNA. Translation: AAA57015.1.
U13780 Genomic DNA. Translation: AAA57016.1.
U13782 Genomic DNA. Translation: AAA57017.1.
U13833 Genomic DNA. Translation: AAA57030.1.
U13834 Genomic DNA. Translation: AAA57031.1.
PIRiF64936. PRECT4.
I81191.
RefSeqiNP_416280.1. NC_000913.3.
WP_001259810.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74836; AAC74836; b1766.
BAA15557; BAA15557; BAA15557.
GeneIDi946281.
KEGGiecj:JW1755.
eco:b1766.
PATRICi32118843. VBIEscCol129921_1839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13359 Genomic DNA. Translation: AAA24648.1.
U00096 Genomic DNA. Translation: AAC74836.1.
AP009048 Genomic DNA. Translation: BAA15557.1.
U13772 Genomic DNA. Translation: AAA57008.1.
U13773 Genomic DNA. Translation: AAA57009.1.
U13774 Genomic DNA. Translation: AAA57010.1.
U13775 Genomic DNA. Translation: AAA57011.1.
U13776 Genomic DNA. Translation: AAA57012.1.
U13777 Genomic DNA. Translation: AAA57013.1.
U13778 Genomic DNA. Translation: AAA57014.1.
U13779 Genomic DNA. Translation: AAA57015.1.
U13780 Genomic DNA. Translation: AAA57016.1.
U13782 Genomic DNA. Translation: AAA57017.1.
U13833 Genomic DNA. Translation: AAA57030.1.
U13834 Genomic DNA. Translation: AAA57031.1.
PIRiF64936. PRECT4.
I81191.
RefSeqiNP_416280.1. NC_000913.3.
WP_001259810.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BEZX-ray2.76A/B/C/D47-618[»]
3BF0X-ray2.55A/B/C/D47-618[»]
ProteinModelPortaliP08395.
SMRiP08395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259140. 4 interactors.
IntActiP08395. 1 interactor.
STRINGi511145.b1766.

Protein family/group databases

MEROPSiS49.001.

Proteomic databases

EPDiP08395.
PaxDbiP08395.
PRIDEiP08395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74836; AAC74836; b1766.
BAA15557; BAA15557; BAA15557.
GeneIDi946281.
KEGGiecj:JW1755.
eco:b1766.
PATRICi32118843. VBIEscCol129921_1839.

Organism-specific databases

EchoBASEiEB0961.
EcoGeneiEG10968. sppA.

Phylogenomic databases

eggNOGiENOG4105D07. Bacteria.
COG0616. LUCA.
InParanoidiP08395.
KOiK04773.
OMAiWSKADNN.
PhylomeDBiP08395.

Enzyme and pathway databases

BioCyciEcoCyc:EG10968-MONOMER.
ECOL316407:JW1755-MONOMER.
MetaCyc:EG10968-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP08395.
PROiP08395.

Family and domain databases

CDDicd07019. S49_SppA_1. 1 hit.
Gene3Di3.90.226.10. 4 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR004634. Pept_S49_pIV.
IPR004635. Pept_S49_SppA.
IPR002142. Peptidase_S49.
IPR033854. S49_SppA_1.
[Graphical view]
PfamiPF01343. Peptidase_S49. 2 hits.
[Graphical view]
PIRSFiPIRSF001217. Protease_4_SppA. 1 hit.
SUPFAMiSSF52096. SSF52096. 4 hits.
TIGRFAMsiTIGR00705. SppA_67K. 1 hit.
TIGR00706. SppA_dom. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPPA_ECOLI
AccessioniPrimary (citable) accession number: P08395
Secondary accession number(s): P77752
, Q46723, Q46724, Q46725, Q46726, Q57183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 29, 2003
Last modified: November 30, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.