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Protein

Protease 4

Gene

sppA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Digests cleaved signal peptides in vitro, its in vivo function is unknown. This activity is necessary to maintain proper secretion of mature proteins across the membrane.4 Publications

Enzyme regulationi

Inhibited by serine hydrolase inhibitor FP-biotin and by antipain.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei209 – 2091Proton donor/acceptorCurated
Active sitei409 – 4091Nucleophile

GO - Molecular functioni

  • endopeptidase activity Source: EcoCyc
  • serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  • signal peptide processing Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10968-MONOMER.
ECOL316407:JW1755-MONOMER.
MetaCyc:EG10968-MONOMER.

Protein family/group databases

MEROPSiS49.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease 4 (EC:3.4.21.-)
Alternative name(s):
Endopeptidase IV
Protease IV
Signal peptide peptidase
Gene namesi
Name:sppA
Ordered Locus Names:b1766, JW1755
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10968. sppA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicAdd
BLAST
Transmembranei25 – 4521HelicalAdd
BLAST
Topological domaini46 – 618573PeriplasmicAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No effect on processing of liberated signal peptides in vivo.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi209 – 2091K → A: Loss of activity. 1 Publication
Mutagenesisi366 – 3661K → A: Reduced activity. 1 Publication
Mutagenesisi409 – 4091S → A: Loss of activity. 1 Publication
Mutagenesisi410 – 4101G → A: Reduced activity. 1 Publication
Mutagenesisi412 – 4121Y → F: No effect on activity. 1 Publication
Mutagenesisi510 – 5101H → A: Reduced activity. 1 Publication
Mutagenesisi524 – 5241D → N: Increased activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Protease 4PRO_0000171438Add
BLAST

Proteomic databases

EPDiP08395.
PaxDbiP08395.
PRIDEiP08395.

Interactioni

Subunit structurei

Homotetramer.2 Publications

Protein-protein interaction databases

BioGridi4259140. 4 interactions.
IntActiP08395. 1 interaction.
STRINGi511145.b1766.

Structurei

Secondary structure

1
618
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi58 – 614Combined sources
Beta strandi64 – 696Combined sources
Beta strandi94 – 963Combined sources
Helixi97 – 10913Combined sources
Beta strandi116 – 1194Combined sources
Beta strandi122 – 1254Combined sources
Helixi128 – 14316Combined sources
Beta strandi148 – 1536Combined sources
Helixi157 – 1637Combined sources
Beta strandi166 – 1716Combined sources
Beta strandi186 – 1883Combined sources
Helixi190 – 1956Combined sources
Beta strandi199 – 2057Combined sources
Helixi210 – 2123Combined sources
Helixi213 – 2164Combined sources
Helixi222 – 24726Combined sources
Helixi251 – 2544Combined sources
Helixi257 – 26610Combined sources
Turni267 – 2704Combined sources
Helixi272 – 2787Combined sources
Beta strandi281 – 2855Combined sources
Helixi288 – 29912Combined sources
Turni303 – 3053Combined sources
Beta strandi306 – 3127Combined sources
Turni313 – 3153Combined sources
Beta strandi327 – 34216Combined sources
Beta strandi346 – 3483Combined sources
Helixi349 – 36113Combined sources
Beta strandi365 – 37713Combined sources
Helixi379 – 39416Combined sources
Beta strandi399 – 40810Combined sources
Helixi410 – 4134Combined sources
Turni414 – 4174Combined sources
Beta strandi419 – 4235Combined sources
Beta strandi428 – 4303Combined sources
Beta strandi434 – 4407Combined sources
Helixi442 – 4476Combined sources
Beta strandi451 – 4533Combined sources
Helixi459 – 4613Combined sources
Helixi471 – 49525Combined sources
Helixi500 – 5045Combined sources
Beta strandi511 – 5133Combined sources
Helixi514 – 5196Combined sources
Beta strandi524 – 5263Combined sources
Helixi529 – 53911Combined sources
Beta strandi545 – 5484Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BEZX-ray2.76A/B/C/D47-618[»]
3BF0X-ray2.55A/B/C/D47-618[»]
ProteinModelPortaliP08395.
SMRiP08395. Positions 56-551.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08395.

Family & Domainsi

Domaini

A tandem duplication in the protein fold creates an intact active site between the repeated domains of each monomer.

Sequence similaritiesi

Belongs to the peptidase S49 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D07. Bacteria.
COG0616. LUCA.
InParanoidiP08395.
KOiK04773.
OMAiWSKADNN.
PhylomeDBiP08395.

Family and domain databases

Gene3Di3.90.226.10. 4 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR004634. Pept_S49_pIV.
IPR004635. Pept_S49_SppA.
IPR002142. Peptidase_S49.
[Graphical view]
PfamiPF01343. Peptidase_S49. 2 hits.
[Graphical view]
PIRSFiPIRSF001217. Protease_4_SppA. 1 hit.
SUPFAMiSSF52096. SSF52096. 4 hits.
TIGRFAMsiTIGR00705. SppA_67K. 1 hit.
TIGR00706. SppA_dom. 1 hit.

Sequencei

Sequence statusi: Complete.

P08395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLWRFIAG FFKWTWRLLN FVREMVLNLF FIFLVLVGVG IWMQVSGGDS
60 70 80 90 100
KETASRGALL LDISGVIVDK PDSSQRFSKL SRQLLGASSD RLQENSLFDI
110 120 130 140 150
VNTIRQAKDD RNITGIVMDL KNFAGGDQPS MQYIGKALKE FRDSGKPVYA
160 170 180 190 200
VGENYSQGQY YLASFANKIW LSPQGVVDLH GFATNGLYYK SLLDKLKVST
210 220 230 240 250
HVFRVGTYKS AVEPFIRDDM SPAAREADSR WIGELWQNYL NTVAANRQIP
260 270 280 290 300
AEQVFPGAQG LLEGLTKTGG DTAKYALENK LVDALASSAE IEKALTKEFG
310 320 330 340 350
WSKTDKNYRA ISYYDYALKT PADTGDSIGV VFANGAIMDG EETQGNVGGD
360 370 380 390 400
TTAAQIRDAR LDPKVKAIVL RVNSPGGSVT ASEVIRAELA AARAAGKPVV
410 420 430 440 450
VSMGGMAASG GYWISTPANY IVANPSTLTG SIGIFGVITT VENSLDSIGV
460 470 480 490 500
HTDGVSTSPL ADVSITRALP PEAQLMMQLS IENGYKRFIT LVADARHSTP
510 520 530 540 550
EQIDKIAQGH VWTGQDAKAN GLVDSLGDFD DAVAKAAELA KVKQWHLEYY
560 570 580 590 600
VDEPTFFDKV MDNMSGSVRA MLPDAFQAML PAPLASVAST VKSESDKLAA
610
FNDPQNRYAF CLTCANMR
Length:618
Mass (Da):67,219
Last modified:August 29, 2003 - v2
Checksum:iEAF00496B5946AC3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti378 – 3781S → T in AAA24648 (PubMed:3522590).Curated
Sequence conflicti378 – 3781S → T in AAA57009 (PubMed:7973728).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti151 – 1511V → I in strain: ECOR 49 and ECOR 50.
Natural varianti186 – 1861G → S in strain: ECOR 16.
Natural varianti252 – 2521E → H in strain: ECOR 16.
Natural varianti252 – 2521E → K in strain: ECOR 49.
Natural varianti252 – 2521E → Q in strain: ECOR 38, ECOR 39, ECOR 40, ECOR 50, ECOR 65 and ECOR 68.
Natural varianti294 – 2941A → T in strain: ECOR 38, ECOR 39, ECOR 40, ECOR 49, ECOR 50 and ECOR 65.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13359 Genomic DNA. Translation: AAA24648.1.
U00096 Genomic DNA. Translation: AAC74836.1.
AP009048 Genomic DNA. Translation: BAA15557.1.
U13772 Genomic DNA. Translation: AAA57008.1.
U13773 Genomic DNA. Translation: AAA57009.1.
U13774 Genomic DNA. Translation: AAA57010.1.
U13775 Genomic DNA. Translation: AAA57011.1.
U13776 Genomic DNA. Translation: AAA57012.1.
U13777 Genomic DNA. Translation: AAA57013.1.
U13778 Genomic DNA. Translation: AAA57014.1.
U13779 Genomic DNA. Translation: AAA57015.1.
U13780 Genomic DNA. Translation: AAA57016.1.
U13782 Genomic DNA. Translation: AAA57017.1.
U13833 Genomic DNA. Translation: AAA57030.1.
U13834 Genomic DNA. Translation: AAA57031.1.
PIRiF64936. PRECT4.
I81191.
RefSeqiNP_416280.1. NC_000913.3.
WP_001259810.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74836; AAC74836; b1766.
BAA15557; BAA15557; BAA15557.
GeneIDi946281.
KEGGiecj:JW1755.
eco:b1766.
PATRICi32118843. VBIEscCol129921_1839.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13359 Genomic DNA. Translation: AAA24648.1.
U00096 Genomic DNA. Translation: AAC74836.1.
AP009048 Genomic DNA. Translation: BAA15557.1.
U13772 Genomic DNA. Translation: AAA57008.1.
U13773 Genomic DNA. Translation: AAA57009.1.
U13774 Genomic DNA. Translation: AAA57010.1.
U13775 Genomic DNA. Translation: AAA57011.1.
U13776 Genomic DNA. Translation: AAA57012.1.
U13777 Genomic DNA. Translation: AAA57013.1.
U13778 Genomic DNA. Translation: AAA57014.1.
U13779 Genomic DNA. Translation: AAA57015.1.
U13780 Genomic DNA. Translation: AAA57016.1.
U13782 Genomic DNA. Translation: AAA57017.1.
U13833 Genomic DNA. Translation: AAA57030.1.
U13834 Genomic DNA. Translation: AAA57031.1.
PIRiF64936. PRECT4.
I81191.
RefSeqiNP_416280.1. NC_000913.3.
WP_001259810.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BEZX-ray2.76A/B/C/D47-618[»]
3BF0X-ray2.55A/B/C/D47-618[»]
ProteinModelPortaliP08395.
SMRiP08395. Positions 56-551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259140. 4 interactions.
IntActiP08395. 1 interaction.
STRINGi511145.b1766.

Protein family/group databases

MEROPSiS49.001.

Proteomic databases

EPDiP08395.
PaxDbiP08395.
PRIDEiP08395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74836; AAC74836; b1766.
BAA15557; BAA15557; BAA15557.
GeneIDi946281.
KEGGiecj:JW1755.
eco:b1766.
PATRICi32118843. VBIEscCol129921_1839.

Organism-specific databases

EchoBASEiEB0961.
EcoGeneiEG10968. sppA.

Phylogenomic databases

eggNOGiENOG4105D07. Bacteria.
COG0616. LUCA.
InParanoidiP08395.
KOiK04773.
OMAiWSKADNN.
PhylomeDBiP08395.

Enzyme and pathway databases

BioCyciEcoCyc:EG10968-MONOMER.
ECOL316407:JW1755-MONOMER.
MetaCyc:EG10968-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP08395.
PROiP08395.

Family and domain databases

Gene3Di3.90.226.10. 4 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR004634. Pept_S49_pIV.
IPR004635. Pept_S49_SppA.
IPR002142. Peptidase_S49.
[Graphical view]
PfamiPF01343. Peptidase_S49. 2 hits.
[Graphical view]
PIRSFiPIRSF001217. Protease_4_SppA. 1 hit.
SUPFAMiSSF52096. SSF52096. 4 hits.
TIGRFAMsiTIGR00705. SppA_67K. 1 hit.
TIGR00706. SppA_dom. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSPPA_ECOLI
AccessioniPrimary (citable) accession number: P08395
Secondary accession number(s): P77752
, Q46723, Q46724, Q46725, Q46726, Q57183
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 29, 2003
Last modified: September 7, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.