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P08394 (EX5B_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exodeoxyribonuclease V beta chain
EC=3.1.11.5
Alternative name(s):
Exodeoxyribonuclease V 135 kDa polypeptide
Gene names
Name:recB
Synonyms:rorA
Ordered Locus Names:b2820, JW2788
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length1180 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for efficient DNA repair; it catalyzes the unwinding of double-stranded DNA and the cleavage of single-stranded DNA and it stimulates local genetic recombination. All of these activities require concomitant hydrolysis of ATP.

Catalytic activity

Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.

Subunit structure

Consists of three subunits; RecB, RecC and RecD. Interacts with YgbT (Cas1). Ref.7

Sequence similarities

Belongs to the helicase family. UvrD subfamily.

Contains 1 uvrD-like helicase ATP-binding domain.

Contains 1 uvrD-like helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11801180Exodeoxyribonuclease V beta chain
PRO_0000102046

Regions

Domain2 – 450449UvrD-like helicase ATP-binding
Domain480 – 746267UvrD-like helicase C-terminal
Nucleotide binding23 – 308ATP

Secondary structure

1 .................................................................................................................................................................................. 1180
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P08394 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: F9AC331808E8F281

FASTA1,180133,959
        10         20         30         40         50         60 
MSDVAETLDP LRLPLQGERL IEASAGTGKT FTIAALYLRL LLGLGGSAAF PRPLTVEELL 

        70         80         90        100        110        120 
VVTFTEAATA ELRGRIRSNI HELRIACLRE TTDNPLYERL LEEIDDKAQA AQWLLLAERQ 

       130        140        150        160        170        180 
MDEAAVFTIH GFCQRMLNLN AFESGMLFEQ QLIEDESLLR YQACADFWRR HCYPLPREIA 

       190        200        210        220        230        240 
QVVFETWKGP QALLRDINRY LQGEAPVIKA PPPDDETLAS RHAQIVARID TVKQQWRDAV 

       250        260        270        280        290        300 
GELDALIESS GIDRRKFNRS NQAKWIDKIS AWAEEETNSY QLPESLEKFS QRFLEDRTKA 

       310        320        330        340        350        360 
GGETPRHPLF EAIDQLLAEP LSIRDLVITR ALAEIRETVA REKRRRGELG FDDMLSRLDS 

       370        380        390        400        410        420 
ALRSESGEVL AAAIRTRFPV AMIDEFQDTD PQQYRIFRRI WHHQPETALL LIGDPKQAIY 

       430        440        450        460        470        480 
AFRGADIFTY MKARSEVHAH YTLDTNWRSA PGMVNSVNKL FSQTDDAFMF REIPFIPVKS 

       490        500        510        520        530        540 
AGKNQALRFV FKGETQPAMK MWLMEGESCG VGDYQSTMAQ VCAAQIRDWL QAGQRGEALL 

       550        560        570        580        590        600 
MNGDDARPVR ASDISVLVRS RQEAAQVRDA LTLLEIPSVY LSNRDSVFET LEAQEMLWLL 

       610        620        630        640        650        660 
QAVMTPEREN TLRSALATSM MGLNALDIET LNNDEHAWDV VVEEFDGYRQ IWRKRGVMPM 

       670        680        690        700        710        720 
LRALMSARNI AENLLATAGG ERRLTDILHI SELLQEAGTQ LESEHALVRW LSQHILEPDS 

       730        740        750        760        770        780 
NASSQQMRLE SDKHLVQIVT IHKSKGLEYP LVWLPFITNF RVQEQAFYHD RHSFEAVLDL 

       790        800        810        820        830        840 
NAAPESVDLA EAERLAEDLR LLYVALTRSV WHCSLGVAPL VRRRGDKKGD TDVHQSALGR 

       850        860        870        880        890        900 
LLQKGEPQDA AGLRTCIEAL CDDDIAWQTA QTGDNQPWQV NDVSTAELNA KTLQRLPGDN 

       910        920        930        940        950        960 
WRVTSYSGLQ QRGHGIAQDL MPRLDVDAAG VASVVEEPTL TPHQFPRGAS PGTFLHSLFE 

       970        980        990       1000       1010       1020 
DLDFTQPVDP NWVREKLELG GFESQWEPVL TEWITAVLQA PLNETGVSLS QLSARNKQVE 

      1030       1040       1050       1060       1070       1080 
MEFYLPISEP LIASQLDTLI RQFDPLSAGC PPLEFMQVRG MLKGFIDLVF RHEGRYYLLD 

      1090       1100       1110       1120       1130       1140 
YKSNWLGEDS SAYTQQAMAA AMQAHRYDLQ YQLYTLALHR YLRHRIADYD YEHHFGGVIY 

      1150       1160       1170       1180 
LFLRGVDKEH PQQGIYTTRP NAGLIALMDE MFAGMTLEEA

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of the Escherichia coli recB gene."
Finch P.W., Storey A., Chapman K.E., Brown K., Hickson I.D., Emmerson P.T.
Nucleic Acids Res. 14:8573-8582(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme."
Arnold D.A., Kowalczykowski S.C.
J. Biol. Chem. 275:12261-12265(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: V1000.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III."
Finch P.W., Wilson R.E., Brown K., Hickson I.D., Emmerson P.T.
Nucleic Acids Res. 14:7695-7703(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
[6]"Complete nucleotide sequence of recD, the structural gene for the alpha subunit of Exonuclease V of Escherichia coli."
Finch P.W., Storey A., Brown K., Hickson I.D., Emmerson P.T.
Nucleic Acids Res. 14:8583-8594(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1093-1180.
[7]"A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair."
Babu M., Beloglazova N., Flick R., Graham C., Skarina T., Nocek B., Gagarinova A., Pogoutse O., Brown G., Binkowski A., Phanse S., Joachimiak A., Koonin E.V., Savchenko A., Emili A., Greenblatt J., Edwards A.M., Yakunin A.F.
Mol. Microbiol. 79:484-502(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YGBT.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X04581 Genomic DNA. Translation: CAA28250.1.
AF179304 Genomic DNA. Translation: AAD56369.1.
U29581 Genomic DNA. Translation: AAB40467.1.
U00096 Genomic DNA. Translation: AAC75859.1.
AP009048 Genomic DNA. Translation: BAE76889.1.
X06227 Genomic DNA. Translation: CAA29577.1.
X04582 Genomic DNA. Translation: CAA28252.1.
PIRNCECX5. A25532.
RefSeqNP_417297.1. NC_000913.2.
YP_491025.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1W36X-ray3.10B/E1-1180[»]
3K70X-ray3.59B/E1-1180[»]
ProteinModelPortalP08394.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-540N.
IntActP08394. 19 interactions.
MINTMINT-1224378.
STRING511145.b2820.

Proteomic databases

PaxDbP08394.
PRIDEP08394.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75859; AAC75859; b2820.
BAE76889; BAE76889; BAE76889.
GeneID12933313.
947286.
KEGGecj:Y75_p2754.
eco:b2820.
PATRIC32121058. VBIEscCol129921_2918.

Organism-specific databases

EchoBASEEB0817.
EcoGeneEG10824. recB.

Phylogenomic databases

eggNOGCOG1074.
HOGENOMHOG000258330.
KOK03582.
OMACEQILVV.
ProtClustDBPRK10876.

Enzyme and pathway databases

BioCycEcoCyc:EG10824-MONOMER.
ECOL316407:JW2788-MONOMER.
MetaCyc:EG10824-MONOMER.

Gene expression databases

GenevestigatorP08394.

Family and domain databases

Gene3D3.90.320.10. 1 hit.
InterProIPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR004586. ExoDNase_V_bsu.
IPR011604. Exonuc_phg/RecB_C.
IPR011335. Restrct_endonuc-II-like.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERPTHR11070. PTHR11070. 1 hit.
PfamPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMSSF52980. Restrict_endonuc_II-like_core. 1 hit.
TIGRFAMsTIGR00609. recB. 1 hit.
PROSITEPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChEMBLCHEMBL1741169.
EvolutionaryTraceP08394.

Entry information

Entry nameEX5B_ECOLI
AccessionPrimary (citable) accession number: P08394
Secondary accession number(s): Q2MA17
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents