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P08392

- ICP4_HHV11

UniProt

P08392 - ICP4_HHV11

Protein

Major viral transcription factor

Gene

ICP4

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Major regulator of viral transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. May interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. Negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi262 – 490229Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. positive regulation of transcription, DNA-templated Source: InterPro
    2. response to type I interferon Source: BHF-UCL
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Major viral transcription factor
    Alternative name(s):
    Alpha-4 protein
    Infected cell protein 4
    Transcriptional activator IE175
    Gene namesi
    Name:ICP4
    Synonyms:IE175
    ORF Names:RS1
    OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
    Taxonomic identifieri10299 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000009294: Genome

    Subcellular locationi

    Host nucleus 1 Publication. Host cytoplasm 1 Publication. Virion tegument 1 Publication
    Note: Localizes to the cytoplasm when phosphorylated.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: CACAO

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12981298Major viral transcription factorPRO_0000115815Add
    BLAST

    Post-translational modificationi

    ADP-ribosylated.
    The long stretch of Ser is a major site of phosphorylation. Only the phosphorylated forms are capable of interacting with beta or gamma genes.1 Publication

    Keywords - PTMi

    ADP-ribosylation, Phosphoprotein

    Proteomic databases

    PRIDEiP08392.

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Homodimer. Interacts with transcriptional regulator ICP27; this interaction is required for proper incorporation of ICP4 into virions.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    UL54P102385EBI-7185388,EBI-6883946

    Protein-protein interaction databases

    IntActiP08392. 3 interactions.
    MINTiMINT-6732561.

    Structurei

    3D structure databases

    ProteinModelPortaliP08392.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi116 – 1216Poly-Asp
    Compositional biasi166 – 1694Poly-Arg
    Compositional biasi176 – 19924Poly-SerAdd
    BLAST
    Compositional biasi236 – 2438Poly-Pro
    Compositional biasi321 – 3244Poly-Pro
    Compositional biasi446 – 4538Poly-Ala
    Compositional biasi502 – 5087Poly-Ala
    Compositional biasi613 – 6164Poly-Pro
    Compositional biasi683 – 6886Poly-Ala
    Compositional biasi702 – 7076Poly-Ala
    Compositional biasi710 – 72213Poly-AlaAdd
    BLAST
    Compositional biasi739 – 7424Poly-Gly
    Compositional biasi788 – 7936Poly-Ala
    Compositional biasi825 – 8317Poly-Ala
    Compositional biasi887 – 8904Poly-Asp

    Sequence similaritiesi

    Belongs to the herpesviridae ICP4 family.Curated

    Family and domain databases

    InterProiIPR005205. Herpes_ICP4_C.
    IPR005206. Herpes_ICP4_N.
    [Graphical view]
    PfamiPF03585. Herpes_ICP4_C. 1 hit.
    PF03584. Herpes_ICP4_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08392-1 [UniParc]FASTAAdd to Basket

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    MASENKQRPG SPGPTDGPPP TPSPDRDERG ALGWGAETEE GGDDPDHDPD     50
    HPHDLDDARR DGRAPAAGTD AGEDAGDAVS PRQLALLASM VEEAVRTIPT 100
    PDPAASPPRT PAFRADDDDG DEYDDAADAA GDRAPARGRE REAPLRGAYP 150
    DPTDRLSPRP PAQPPRRRRH GRWRPSASST SSDSGSSSSS SASSSSSSSD 200
    EDEDDDGNDA ADHAREARAV GRGPSSAAPA APGRTPPPPG PPPLSEAAPK 250
    PRAAARTPAA SAGRIERRRA RAAVAGRDAT GRFTAGQPRR VELDADATSG 300
    AFYARYRDGY VSGEPWPGAG PPPPGRVLYG GLGDSRPGLW GAPEAEEARR 350
    RFEASGAPAA VWAPELGDAA QQYALITRLL YTPDAEAMGW LQNPRVVPGD 400
    VALDQACFRI SGAARNSSSF ITGSVARAVP HLGYAMAAGR FGWGLAHAAA 450
    AVAMSRRYDR AQKGFLLTSL RRAYAPLLAR ENAALTGAAG SPGAGADDEG 500
    VAAVAAAAPG ERAVPAGYGA AGILAALGRL SAAPASPAGG DDPDAARHAD 550
    ADDDAGRRAQ AGRVAVECLA ACRGILEALA EGFDGDLAAV PGLAGARPAS 600
    PPRPEGPAGP ASPPPPHADA PRLRAWLREL RFVRDALVLM RLRGDLRVAG 650
    GSEAAVAAVR AVSLVAGALG PALPRDPRLP SSAAAAAADL LFDNQSLRPL 700
    LAAAASAPDA ADALAAAAAS AAPREGRKRK SPGPARPPGG GGPRPPKTKK 750
    SGADAPGSDA RAPLPAPAPP STPPGPEPAP AQPAAPRAAA AQARPRPVAV 800
    SRRPAEGPDP LGGWRRQPPG PSHTAAPAAA ALEAYCSPRA VAELTDHPLF 850
    PVPWRPALMF DPRALASIAA RCAGPAPAAQ AACGGGDDDD NPHPHGAAGG 900
    RLFGPLRASG PLRRMAAWMR QIPDPEDVRV VVLYSPLPGE DLAGGGASGG 950
    PPEWSAERGG LSCLLAALAN RLCGPDTAAW AGNWTGAPDV SALGAQGVLL 1000
    LSTRDLAFAG AVEFLGLLAS AGDRRLIVVN TVRACDWPAD GPAVSRQHAY 1050
    LACELLPAVQ CAVRWPAARD LRRTVLASGR VFGPGVFARV EAAHARLYPD 1100
    APPLRLCRGG NVRYRVRTRF GPDTPVPMSP REYRRAVLPA LDGRAAASGT 1150
    TDAMAPGAPD FCEEEAHSHA ACARWGLGAP LRPVYVALGR EAVRAGPARW 1200
    RGPRRDFCAR ALLEPDDDAP PLVLRGDDDG PGALPPAPPG IRWASATGRS 1250
    GTVLAAAGAV EVLGAEAGLA TPPRREVVDW EGAWDEDDGG AFEGDGVL 1298
    Length:1,298
    Mass (Da):132,844
    Last modified:August 1, 1988 - v1
    Checksum:i4F32E04C95CA9344
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32286.1.
    X14112 Genomic DNA. Translation: CAA32278.1.
    X06461 Genomic DNA. Translation: CAA29763.1.
    L00036 Genomic DNA. Translation: AAA96675.1.
    L00036 Genomic DNA. Translation: AAA96688.1.
    PIRiA23510. EDBE75.
    RefSeqiNP_044662.1. NC_001806.1.
    NP_044676.1. NC_001806.1.

    Genome annotation databases

    GeneIDi2703391.
    2703392.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14112 Genomic DNA. Translation: CAA32286.1 .
    X14112 Genomic DNA. Translation: CAA32278.1 .
    X06461 Genomic DNA. Translation: CAA29763.1 .
    L00036 Genomic DNA. Translation: AAA96675.1 .
    L00036 Genomic DNA. Translation: AAA96688.1 .
    PIRi A23510. EDBE75.
    RefSeqi NP_044662.1. NC_001806.1.
    NP_044676.1. NC_001806.1.

    3D structure databases

    ProteinModelPortali P08392.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08392. 3 interactions.
    MINTi MINT-6732561.

    Proteomic databases

    PRIDEi P08392.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2703391.
    2703392.

    Family and domain databases

    InterProi IPR005205. Herpes_ICP4_C.
    IPR005206. Herpes_ICP4_N.
    [Graphical view ]
    Pfami PF03585. Herpes_ICP4_C. 1 hit.
    PF03584. Herpes_ICP4_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1."
      McGeoch D.J., Dolan A., Donald S., Brauer D.H.K.
      Nucleic Acids Res. 14:1727-1745(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
      McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
      J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Codons 262 to 490 from the herpes simplex virus ICP4 gene are sufficient to encode a sequence-specific DNA binding protein."
      Wu C.-L., Wilcox K.W.
      Nucleic Acids Res. 18:531-538(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING DOMAIN.
    4. "The interaction of ICP4 with cell/infected-cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA."
      Papavassiliou A.G., Wilcox K.W., Silverstein S.J.
      EMBO J. 10:397-406(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION.
    5. "Physical and functional interactions between herpes simplex virus immediate-early proteins ICP4 and ICP27."
      Panagiotidis C.A., Lium E.K., Silverstein S.J.
      J. Virol. 71:1547-1557(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ICP27.
    6. "Herpes simplex virus type 1 immediate-early protein ICP27 is required for efficient incorporation of ICP0 and ICP4 into virions."
      Sedlackova L., Rice S.A.
      J. Virol. 82:268-277(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiICP4_HHV11
    AccessioniPrimary (citable) accession number: P08392
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3