Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P08392

- ICP4_HHV11

UniProt

P08392 - ICP4_HHV11

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Major viral transcription factor

Gene

ICP4

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Major regulator of viral transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. May interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. Negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi262 – 490229Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of transcription, DNA-templated Source: InterPro
  2. response to type I interferon Source: BHF-UCL
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Major viral transcription factor
Alternative name(s):
Alpha-4 protein
Infected cell protein 4
Transcriptional activator IE175
Gene namesi
Name:ICP4
Synonyms:IE175
ORF Names:RS1
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294: Genome

Subcellular locationi

Host nucleus 1 Publication. Host cytoplasm 1 Publication. Virion tegument 1 Publication
Note: Localizes to the cytoplasm when phosphorylated.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral tegument Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12981298Major viral transcription factorPRO_0000115815Add
BLAST

Post-translational modificationi

ADP-ribosylated.
The long stretch of Ser is a major site of phosphorylation. Only the phosphorylated forms are capable of interacting with beta or gamma genes.1 Publication

Keywords - PTMi

ADP-ribosylation, Phosphoprotein

Proteomic databases

PRIDEiP08392.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer. Interacts with transcriptional regulator ICP27; this interaction is required for proper incorporation of ICP4 into virions.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
UL54P102385EBI-7185388,EBI-6883946

Protein-protein interaction databases

IntActiP08392. 3 interactions.
MINTiMINT-6732561.

Structurei

3D structure databases

ProteinModelPortaliP08392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi116 – 1216Poly-Asp
Compositional biasi166 – 1694Poly-Arg
Compositional biasi176 – 19924Poly-SerAdd
BLAST
Compositional biasi236 – 2438Poly-Pro
Compositional biasi321 – 3244Poly-Pro
Compositional biasi446 – 4538Poly-Ala
Compositional biasi502 – 5087Poly-Ala
Compositional biasi613 – 6164Poly-Pro
Compositional biasi683 – 6886Poly-Ala
Compositional biasi702 – 7076Poly-Ala
Compositional biasi710 – 72213Poly-AlaAdd
BLAST
Compositional biasi739 – 7424Poly-Gly
Compositional biasi788 – 7936Poly-Ala
Compositional biasi825 – 8317Poly-Ala
Compositional biasi887 – 8904Poly-Asp

Sequence similaritiesi

Belongs to the herpesviridae ICP4 family.Curated

Family and domain databases

InterProiIPR005205. Herpes_ICP4_C.
IPR005206. Herpes_ICP4_N.
[Graphical view]
PfamiPF03585. Herpes_ICP4_C. 1 hit.
PF03584. Herpes_ICP4_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08392-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASENKQRPG SPGPTDGPPP TPSPDRDERG ALGWGAETEE GGDDPDHDPD
60 70 80 90 100
HPHDLDDARR DGRAPAAGTD AGEDAGDAVS PRQLALLASM VEEAVRTIPT
110 120 130 140 150
PDPAASPPRT PAFRADDDDG DEYDDAADAA GDRAPARGRE REAPLRGAYP
160 170 180 190 200
DPTDRLSPRP PAQPPRRRRH GRWRPSASST SSDSGSSSSS SASSSSSSSD
210 220 230 240 250
EDEDDDGNDA ADHAREARAV GRGPSSAAPA APGRTPPPPG PPPLSEAAPK
260 270 280 290 300
PRAAARTPAA SAGRIERRRA RAAVAGRDAT GRFTAGQPRR VELDADATSG
310 320 330 340 350
AFYARYRDGY VSGEPWPGAG PPPPGRVLYG GLGDSRPGLW GAPEAEEARR
360 370 380 390 400
RFEASGAPAA VWAPELGDAA QQYALITRLL YTPDAEAMGW LQNPRVVPGD
410 420 430 440 450
VALDQACFRI SGAARNSSSF ITGSVARAVP HLGYAMAAGR FGWGLAHAAA
460 470 480 490 500
AVAMSRRYDR AQKGFLLTSL RRAYAPLLAR ENAALTGAAG SPGAGADDEG
510 520 530 540 550
VAAVAAAAPG ERAVPAGYGA AGILAALGRL SAAPASPAGG DDPDAARHAD
560 570 580 590 600
ADDDAGRRAQ AGRVAVECLA ACRGILEALA EGFDGDLAAV PGLAGARPAS
610 620 630 640 650
PPRPEGPAGP ASPPPPHADA PRLRAWLREL RFVRDALVLM RLRGDLRVAG
660 670 680 690 700
GSEAAVAAVR AVSLVAGALG PALPRDPRLP SSAAAAAADL LFDNQSLRPL
710 720 730 740 750
LAAAASAPDA ADALAAAAAS AAPREGRKRK SPGPARPPGG GGPRPPKTKK
760 770 780 790 800
SGADAPGSDA RAPLPAPAPP STPPGPEPAP AQPAAPRAAA AQARPRPVAV
810 820 830 840 850
SRRPAEGPDP LGGWRRQPPG PSHTAAPAAA ALEAYCSPRA VAELTDHPLF
860 870 880 890 900
PVPWRPALMF DPRALASIAA RCAGPAPAAQ AACGGGDDDD NPHPHGAAGG
910 920 930 940 950
RLFGPLRASG PLRRMAAWMR QIPDPEDVRV VVLYSPLPGE DLAGGGASGG
960 970 980 990 1000
PPEWSAERGG LSCLLAALAN RLCGPDTAAW AGNWTGAPDV SALGAQGVLL
1010 1020 1030 1040 1050
LSTRDLAFAG AVEFLGLLAS AGDRRLIVVN TVRACDWPAD GPAVSRQHAY
1060 1070 1080 1090 1100
LACELLPAVQ CAVRWPAARD LRRTVLASGR VFGPGVFARV EAAHARLYPD
1110 1120 1130 1140 1150
APPLRLCRGG NVRYRVRTRF GPDTPVPMSP REYRRAVLPA LDGRAAASGT
1160 1170 1180 1190 1200
TDAMAPGAPD FCEEEAHSHA ACARWGLGAP LRPVYVALGR EAVRAGPARW
1210 1220 1230 1240 1250
RGPRRDFCAR ALLEPDDDAP PLVLRGDDDG PGALPPAPPG IRWASATGRS
1260 1270 1280 1290
GTVLAAAGAV EVLGAEAGLA TPPRREVVDW EGAWDEDDGG AFEGDGVL
Length:1,298
Mass (Da):132,844
Last modified:August 1, 1988 - v1
Checksum:i4F32E04C95CA9344
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32286.1.
X14112 Genomic DNA. Translation: CAA32278.1.
X06461 Genomic DNA. Translation: CAA29763.1.
L00036 Genomic DNA. Translation: AAA96675.1.
L00036 Genomic DNA. Translation: AAA96688.1.
PIRiA23510. EDBE75.
RefSeqiNP_044662.1. NC_001806.1.
NP_044676.1. NC_001806.1.

Genome annotation databases

GeneIDi2703391.
2703392.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32286.1 .
X14112 Genomic DNA. Translation: CAA32278.1 .
X06461 Genomic DNA. Translation: CAA29763.1 .
L00036 Genomic DNA. Translation: AAA96675.1 .
L00036 Genomic DNA. Translation: AAA96688.1 .
PIRi A23510. EDBE75.
RefSeqi NP_044662.1. NC_001806.1.
NP_044676.1. NC_001806.1.

3D structure databases

ProteinModelPortali P08392.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08392. 3 interactions.
MINTi MINT-6732561.

Proteomic databases

PRIDEi P08392.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703391.
2703392.

Family and domain databases

InterProi IPR005205. Herpes_ICP4_C.
IPR005206. Herpes_ICP4_N.
[Graphical view ]
Pfami PF03585. Herpes_ICP4_C. 1 hit.
PF03584. Herpes_ICP4_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dolan A., Donald S., Brauer D.H.K.
    Nucleic Acids Res. 14:1727-1745(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Codons 262 to 490 from the herpes simplex virus ICP4 gene are sufficient to encode a sequence-specific DNA binding protein."
    Wu C.-L., Wilcox K.W.
    Nucleic Acids Res. 18:531-538(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING DOMAIN.
  4. "The interaction of ICP4 with cell/infected-cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA."
    Papavassiliou A.G., Wilcox K.W., Silverstein S.J.
    EMBO J. 10:397-406(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  5. "Physical and functional interactions between herpes simplex virus immediate-early proteins ICP4 and ICP27."
    Panagiotidis C.A., Lium E.K., Silverstein S.J.
    J. Virol. 71:1547-1557(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ICP27.
  6. "Herpes simplex virus type 1 immediate-early protein ICP27 is required for efficient incorporation of ICP0 and ICP4 into virions."
    Sedlackova L., Rice S.A.
    J. Virol. 82:268-277(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiICP4_HHV11
AccessioniPrimary (citable) accession number: P08392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3