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P08392 (ICP4_HHV11) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Major viral transcription factor
Alternative name(s):
Alpha-4 protein
Infected cell protein 4
Transcriptional activator IE175
Gene names
Name:ICP4
Synonyms:IE175
ORF Names:RS1
OrganismHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]
Taxonomic identifier10299 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length1298 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major regulator of viral transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. May interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. Negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis. Ref.4

Subunit structure

Homodimer. Interacts with transcriptional regulator ICP27; this interaction is required for proper incorporation of ICP4 into virions. Ref.5

Subcellular location

Host nucleus. Host cytoplasm. Virion tegument. Note: Localizes to the cytoplasm when phosphorylated By similarity. Ref.6

Post-translational modification

ADP-ribosylated.

The long stretch of Ser is a major site of phosphorylation. Only the phosphorylated forms are capable of interacting with beta or gamma genes. Ref.4

Sequence similarities

Belongs to the herpesviridae ICP4 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

UL54P102385EBI-7185388,EBI-6883946

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12981298Major viral transcription factor
PRO_0000115815

Regions

DNA binding262 – 490229 Ref.3
Compositional bias116 – 1216Poly-Asp
Compositional bias166 – 1694Poly-Arg
Compositional bias176 – 19924Poly-Ser
Compositional bias236 – 2438Poly-Pro
Compositional bias321 – 3244Poly-Pro
Compositional bias446 – 4538Poly-Ala
Compositional bias502 – 5087Poly-Ala
Compositional bias613 – 6164Poly-Pro
Compositional bias683 – 6886Poly-Ala
Compositional bias702 – 7076Poly-Ala
Compositional bias710 – 72213Poly-Ala
Compositional bias739 – 7424Poly-Gly
Compositional bias788 – 7936Poly-Ala
Compositional bias825 – 8317Poly-Ala
Compositional bias887 – 8904Poly-Asp

Sequences

Sequence LengthMass (Da)Tools
P08392 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 4F32E04C95CA9344

FASTA1,298132,844
        10         20         30         40         50         60 
MASENKQRPG SPGPTDGPPP TPSPDRDERG ALGWGAETEE GGDDPDHDPD HPHDLDDARR 

        70         80         90        100        110        120 
DGRAPAAGTD AGEDAGDAVS PRQLALLASM VEEAVRTIPT PDPAASPPRT PAFRADDDDG 

       130        140        150        160        170        180 
DEYDDAADAA GDRAPARGRE REAPLRGAYP DPTDRLSPRP PAQPPRRRRH GRWRPSASST 

       190        200        210        220        230        240 
SSDSGSSSSS SASSSSSSSD EDEDDDGNDA ADHAREARAV GRGPSSAAPA APGRTPPPPG 

       250        260        270        280        290        300 
PPPLSEAAPK PRAAARTPAA SAGRIERRRA RAAVAGRDAT GRFTAGQPRR VELDADATSG 

       310        320        330        340        350        360 
AFYARYRDGY VSGEPWPGAG PPPPGRVLYG GLGDSRPGLW GAPEAEEARR RFEASGAPAA 

       370        380        390        400        410        420 
VWAPELGDAA QQYALITRLL YTPDAEAMGW LQNPRVVPGD VALDQACFRI SGAARNSSSF 

       430        440        450        460        470        480 
ITGSVARAVP HLGYAMAAGR FGWGLAHAAA AVAMSRRYDR AQKGFLLTSL RRAYAPLLAR 

       490        500        510        520        530        540 
ENAALTGAAG SPGAGADDEG VAAVAAAAPG ERAVPAGYGA AGILAALGRL SAAPASPAGG 

       550        560        570        580        590        600 
DDPDAARHAD ADDDAGRRAQ AGRVAVECLA ACRGILEALA EGFDGDLAAV PGLAGARPAS 

       610        620        630        640        650        660 
PPRPEGPAGP ASPPPPHADA PRLRAWLREL RFVRDALVLM RLRGDLRVAG GSEAAVAAVR 

       670        680        690        700        710        720 
AVSLVAGALG PALPRDPRLP SSAAAAAADL LFDNQSLRPL LAAAASAPDA ADALAAAAAS 

       730        740        750        760        770        780 
AAPREGRKRK SPGPARPPGG GGPRPPKTKK SGADAPGSDA RAPLPAPAPP STPPGPEPAP 

       790        800        810        820        830        840 
AQPAAPRAAA AQARPRPVAV SRRPAEGPDP LGGWRRQPPG PSHTAAPAAA ALEAYCSPRA 

       850        860        870        880        890        900 
VAELTDHPLF PVPWRPALMF DPRALASIAA RCAGPAPAAQ AACGGGDDDD NPHPHGAAGG 

       910        920        930        940        950        960 
RLFGPLRASG PLRRMAAWMR QIPDPEDVRV VVLYSPLPGE DLAGGGASGG PPEWSAERGG 

       970        980        990       1000       1010       1020 
LSCLLAALAN RLCGPDTAAW AGNWTGAPDV SALGAQGVLL LSTRDLAFAG AVEFLGLLAS 

      1030       1040       1050       1060       1070       1080 
AGDRRLIVVN TVRACDWPAD GPAVSRQHAY LACELLPAVQ CAVRWPAARD LRRTVLASGR 

      1090       1100       1110       1120       1130       1140 
VFGPGVFARV EAAHARLYPD APPLRLCRGG NVRYRVRTRF GPDTPVPMSP REYRRAVLPA 

      1150       1160       1170       1180       1190       1200 
LDGRAAASGT TDAMAPGAPD FCEEEAHSHA ACARWGLGAP LRPVYVALGR EAVRAGPARW 

      1210       1220       1230       1240       1250       1260 
RGPRRDFCAR ALLEPDDDAP PLVLRGDDDG PGALPPAPPG IRWASATGRS GTVLAAAGAV 

      1270       1280       1290 
EVLGAEAGLA TPPRREVVDW EGAWDEDDGG AFEGDGVL 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dolan A., Donald S., Brauer D.H.K.
Nucleic Acids Res. 14:1727-1745(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Codons 262 to 490 from the herpes simplex virus ICP4 gene are sufficient to encode a sequence-specific DNA binding protein."
Wu C.-L., Wilcox K.W.
Nucleic Acids Res. 18:531-538(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING DOMAIN.
[4]"The interaction of ICP4 with cell/infected-cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA."
Papavassiliou A.G., Wilcox K.W., Silverstein S.J.
EMBO J. 10:397-406(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION.
[5]"Physical and functional interactions between herpes simplex virus immediate-early proteins ICP4 and ICP27."
Panagiotidis C.A., Lium E.K., Silverstein S.J.
J. Virol. 71:1547-1557(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ICP27.
[6]"Herpes simplex virus type 1 immediate-early protein ICP27 is required for efficient incorporation of ICP0 and ICP4 into virions."
Sedlackova L., Rice S.A.
J. Virol. 82:268-277(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14112 Genomic DNA. Translation: CAA32286.1.
X14112 Genomic DNA. Translation: CAA32278.1.
X06461 Genomic DNA. Translation: CAA29763.1.
L00036 Genomic DNA. Translation: AAA96675.1.
L00036 Genomic DNA. Translation: AAA96688.1.
PIREDBE75. A23510.
RefSeqNP_044662.1. NC_001806.1.
NP_044676.1. NC_001806.1.

3D structure databases

ProteinModelPortalP08392.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP08392. 3 interactions.
MINTMINT-6732561.

Proteomic databases

PRIDEP08392.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2703391.
2703392.

Phylogenomic databases

ProtClustDBPHA3307.

Family and domain databases

InterProIPR005205. Herpes_ICP4_C.
IPR005206. Herpes_ICP4_N.
[Graphical view]
PfamPF03585. Herpes_ICP4_C. 1 hit.
PF03584. Herpes_ICP4_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameICP4_HHV11
AccessionPrimary (citable) accession number: P08392
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families