P08392 (ICP4_HHV11) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trans-acting transcriptional protein ICP4

Alternative name(s):
Alpha-4 protein
Infected cell protein 4
Transcriptional activator IE175

Gene names

Name:	ICP4
Synonyms:	IE175
ORF Names:	RS1

Organism

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) [Reference proteome]

Taxonomic identifier

10299 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Simplexvirus › Human herpesvirus 1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1298 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional transactivator that binds with high affinity to the sequence 5'-ATCGTC-3'. May interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. Negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis. Ref.4
Subunit structure	Homodimer. Interacts with transcriptional regulator ICP27; this interaction is required for proper incorporation of ICP4 into virions. Ref.5
Subcellular location	Host nucleus. Host cytoplasm. Virion tegument. Note: Localizes to the cytoplasm when phosphorylated By similarity. Ref.6
Post-translational modification	ADP-ribosylated. The long stretch of Ser is a major site of phosphorylation. Only the phosphorylated forms are capable of interacting with beta or gamma genes. Ref.4
Sequence similarities	Belongs to the herpesviridae ICP4/IE140/IE180 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Host cytoplasm Host nucleus Virion Virion tegument
Developmental stage	Early protein
Ligand	DNA-binding
Molecular function	Activator
PTM	ADP-ribosylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to type I interferon Inferred from direct assay PubMed 16873258. Source: BHF-UCL transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral tegument Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1298	1298	Trans-acting transcriptional protein ICP4		PRO_0000115815
Regions
	DNA binding	262 – 490	229	Ref.3
	Compositional bias	116 – 121	6	Poly-Asp
	Compositional bias	166 – 169	4	Poly-Arg
	Compositional bias	176 – 199	24	Poly-Ser
	Compositional bias	236 – 243	8	Poly-Pro
	Compositional bias	321 – 324	4	Poly-Pro
	Compositional bias	446 – 453	8	Poly-Ala
	Compositional bias	502 – 508	7	Poly-Ala
	Compositional bias	613 – 616	4	Poly-Pro
	Compositional bias	683 – 688	6	Poly-Ala
	Compositional bias	702 – 707	6	Poly-Ala
	Compositional bias	710 – 722	13	Poly-Ala
	Compositional bias	739 – 742	4	Poly-Gly
	Compositional bias	788 – 793	6	Poly-Ala
	Compositional bias	825 – 831	7	Poly-Ala
	Compositional bias	887 – 890	4	Poly-Asp

Sequences

	Sequence		Length	Mass (Da)
	P08392 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 4F32E04C95CA9344	FASTA	1,298	132,844
	10 20 30 40 50 60 MASENKQRPG SPGPTDGPPP TPSPDRDERG ALGWGAETEE GGDDPDHDPD HPHDLDDARR 70 80 90 100 110 120 DGRAPAAGTD AGEDAGDAVS PRQLALLASM VEEAVRTIPT PDPAASPPRT PAFRADDDDG 130 140 150 160 170 180 DEYDDAADAA GDRAPARGRE REAPLRGAYP DPTDRLSPRP PAQPPRRRRH GRWRPSASST 190 200 210 220 230 240 SSDSGSSSSS SASSSSSSSD EDEDDDGNDA ADHAREARAV GRGPSSAAPA APGRTPPPPG 250 260 270 280 290 300 PPPLSEAAPK PRAAARTPAA SAGRIERRRA RAAVAGRDAT GRFTAGQPRR VELDADATSG 310 320 330 340 350 360 AFYARYRDGY VSGEPWPGAG PPPPGRVLYG GLGDSRPGLW GAPEAEEARR RFEASGAPAA 370 380 390 400 410 420 VWAPELGDAA QQYALITRLL YTPDAEAMGW LQNPRVVPGD VALDQACFRI SGAARNSSSF 430 440 450 460 470 480 ITGSVARAVP HLGYAMAAGR FGWGLAHAAA AVAMSRRYDR AQKGFLLTSL RRAYAPLLAR 490 500 510 520 530 540 ENAALTGAAG SPGAGADDEG VAAVAAAAPG ERAVPAGYGA AGILAALGRL SAAPASPAGG 550 560 570 580 590 600 DDPDAARHAD ADDDAGRRAQ AGRVAVECLA ACRGILEALA EGFDGDLAAV PGLAGARPAS 610 620 630 640 650 660 PPRPEGPAGP ASPPPPHADA PRLRAWLREL RFVRDALVLM RLRGDLRVAG GSEAAVAAVR 670 680 690 700 710 720 AVSLVAGALG PALPRDPRLP SSAAAAAADL LFDNQSLRPL LAAAASAPDA ADALAAAAAS 730 740 750 760 770 780 AAPREGRKRK SPGPARPPGG GGPRPPKTKK SGADAPGSDA RAPLPAPAPP STPPGPEPAP 790 800 810 820 830 840 AQPAAPRAAA AQARPRPVAV SRRPAEGPDP LGGWRRQPPG PSHTAAPAAA ALEAYCSPRA 850 860 870 880 890 900 VAELTDHPLF PVPWRPALMF DPRALASIAA RCAGPAPAAQ AACGGGDDDD NPHPHGAAGG 910 920 930 940 950 960 RLFGPLRASG PLRRMAAWMR QIPDPEDVRV VVLYSPLPGE DLAGGGASGG PPEWSAERGG 970 980 990 1000 1010 1020 LSCLLAALAN RLCGPDTAAW AGNWTGAPDV SALGAQGVLL LSTRDLAFAG AVEFLGLLAS 1030 1040 1050 1060 1070 1080 AGDRRLIVVN TVRACDWPAD GPAVSRQHAY LACELLPAVQ CAVRWPAARD LRRTVLASGR 1090 1100 1110 1120 1130 1140 VFGPGVFARV EAAHARLYPD APPLRLCRGG NVRYRVRTRF GPDTPVPMSP REYRRAVLPA 1150 1160 1170 1180 1190 1200 LDGRAAASGT TDAMAPGAPD FCEEEAHSHA ACARWGLGAP LRPVYVALGR EAVRAGPARW 1210 1220 1230 1240 1250 1260 RGPRRDFCAR ALLEPDDDAP PLVLRGDDDG PGALPPAPPG IRWASATGRS GTVLAAAGAV 1270 1280 1290 EVLGAEAGLA TPPRREVVDW EGAWDEDDGG AFEGDGVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1." McGeoch D.J., Dolan A., Donald S., Brauer D.H.K. Nucleic Acids Res. 14:1727-1745(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1." McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P. J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Codons 262 to 490 from the herpes simplex virus ICP4 gene are sufficient to encode a sequence-specific DNA binding protein." Wu C.-L., Wilcox K.W. Nucleic Acids Res. 18:531-538(1990) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING DOMAIN.
[4]	"The interaction of ICP4 with cell/infected-cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA." Papavassiliou A.G., Wilcox K.W., Silverstein S.J. EMBO J. 10:397-406(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION.
[5]	"Physical and functional interactions between herpes simplex virus immediate-early proteins ICP4 and ICP27." Panagiotidis C.A., Lium E.K., Silverstein S.J. J. Virol. 71:1547-1557(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ICP27.
[6]	"Herpes simplex virus type 1 immediate-early protein ICP27 is required for efficient incorporation of ICP0 and ICP4 into virions." Sedlackova L., Rice S.A. J. Virol. 82:268-277(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14112 Genomic DNA. Translation: CAA32286.1. X14112 Genomic DNA. Translation: CAA32278.1. X06461 Genomic DNA. Translation: CAA29763.1. L00036 Genomic DNA. Translation: AAA96675.1. L00036 Genomic DNA. Translation: AAA96688.1.
PIR	EDBE75. A23510.
RefSeq	NP_044662.1. NC_001806.1. NP_044676.1. NC_001806.1.
3D structure databases
ProteinModelPortal	P08392.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6732561.
Proteomic databases
PRIDE	P08392.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2703391. 2703392.
Phylogenomic databases
ProtClustDB	PHA3307.
Family and domain databases
InterPro	IPR005205. Herpes_ICP4_C. IPR005206. Herpes_ICP4_N. [Graphical view]
Pfam	PF03585. Herpes_ICP4_C. 1 hit. PF03584. Herpes_ICP4_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICP4_HHV11

Accession

Primary (citable) accession number: P08392

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program