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P08392

- ICP4_HHV11

UniProt

P08392 - ICP4_HHV11

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Protein
Major viral transcription factor
Gene
ICP4, IE175, RS1
Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Major regulator of viral transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. May interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. Negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi262 – 4902291 Publication
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. protein binding Source: IntAct

GO - Biological processi

  1. positive regulation of transcription, DNA-templated Source: InterPro
  2. response to type I interferon Source: BHF-UCL
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Major viral transcription factor
Alternative name(s):
Alpha-4 protein
Infected cell protein 4
Transcriptional activator IE175
Gene namesi
Name:ICP4
Synonyms:IE175
ORF Names:RS1
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009294: Genome

Subcellular locationi

Host nucleus. Host cytoplasm. Virion tegument
Note: Localizes to the cytoplasm when phosphorylated By similarity.1 Publication

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12981298Major viral transcription factor
PRO_0000115815Add
BLAST

Post-translational modificationi

ADP-ribosylated.
The long stretch of Ser is a major site of phosphorylation. Only the phosphorylated forms are capable of interacting with beta or gamma genes.1 Publication

Keywords - PTMi

ADP-ribosylation, Phosphoprotein

Proteomic databases

PRIDEiP08392.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer. Interacts with transcriptional regulator ICP27; this interaction is required for proper incorporation of ICP4 into virions.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
UL54P102385EBI-7185388,EBI-6883946

Protein-protein interaction databases

IntActiP08392. 3 interactions.
MINTiMINT-6732561.

Structurei

3D structure databases

ProteinModelPortaliP08392.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi116 – 1216Poly-Asp
Compositional biasi166 – 1694Poly-Arg
Compositional biasi176 – 19924Poly-Ser
Add
BLAST
Compositional biasi236 – 2438Poly-Pro
Compositional biasi321 – 3244Poly-Pro
Compositional biasi446 – 4538Poly-Ala
Compositional biasi502 – 5087Poly-Ala
Compositional biasi613 – 6164Poly-Pro
Compositional biasi683 – 6886Poly-Ala
Compositional biasi702 – 7076Poly-Ala
Compositional biasi710 – 72213Poly-Ala
Add
BLAST
Compositional biasi739 – 7424Poly-Gly
Compositional biasi788 – 7936Poly-Ala
Compositional biasi825 – 8317Poly-Ala
Compositional biasi887 – 8904Poly-Asp

Sequence similaritiesi

Family and domain databases

InterProiIPR005205. Herpes_ICP4_C.
IPR005206. Herpes_ICP4_N.
[Graphical view]
PfamiPF03585. Herpes_ICP4_C. 1 hit.
PF03584. Herpes_ICP4_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08392-1 [UniParc]FASTAAdd to Basket

« Hide

MASENKQRPG SPGPTDGPPP TPSPDRDERG ALGWGAETEE GGDDPDHDPD     50
HPHDLDDARR DGRAPAAGTD AGEDAGDAVS PRQLALLASM VEEAVRTIPT 100
PDPAASPPRT PAFRADDDDG DEYDDAADAA GDRAPARGRE REAPLRGAYP 150
DPTDRLSPRP PAQPPRRRRH GRWRPSASST SSDSGSSSSS SASSSSSSSD 200
EDEDDDGNDA ADHAREARAV GRGPSSAAPA APGRTPPPPG PPPLSEAAPK 250
PRAAARTPAA SAGRIERRRA RAAVAGRDAT GRFTAGQPRR VELDADATSG 300
AFYARYRDGY VSGEPWPGAG PPPPGRVLYG GLGDSRPGLW GAPEAEEARR 350
RFEASGAPAA VWAPELGDAA QQYALITRLL YTPDAEAMGW LQNPRVVPGD 400
VALDQACFRI SGAARNSSSF ITGSVARAVP HLGYAMAAGR FGWGLAHAAA 450
AVAMSRRYDR AQKGFLLTSL RRAYAPLLAR ENAALTGAAG SPGAGADDEG 500
VAAVAAAAPG ERAVPAGYGA AGILAALGRL SAAPASPAGG DDPDAARHAD 550
ADDDAGRRAQ AGRVAVECLA ACRGILEALA EGFDGDLAAV PGLAGARPAS 600
PPRPEGPAGP ASPPPPHADA PRLRAWLREL RFVRDALVLM RLRGDLRVAG 650
GSEAAVAAVR AVSLVAGALG PALPRDPRLP SSAAAAAADL LFDNQSLRPL 700
LAAAASAPDA ADALAAAAAS AAPREGRKRK SPGPARPPGG GGPRPPKTKK 750
SGADAPGSDA RAPLPAPAPP STPPGPEPAP AQPAAPRAAA AQARPRPVAV 800
SRRPAEGPDP LGGWRRQPPG PSHTAAPAAA ALEAYCSPRA VAELTDHPLF 850
PVPWRPALMF DPRALASIAA RCAGPAPAAQ AACGGGDDDD NPHPHGAAGG 900
RLFGPLRASG PLRRMAAWMR QIPDPEDVRV VVLYSPLPGE DLAGGGASGG 950
PPEWSAERGG LSCLLAALAN RLCGPDTAAW AGNWTGAPDV SALGAQGVLL 1000
LSTRDLAFAG AVEFLGLLAS AGDRRLIVVN TVRACDWPAD GPAVSRQHAY 1050
LACELLPAVQ CAVRWPAARD LRRTVLASGR VFGPGVFARV EAAHARLYPD 1100
APPLRLCRGG NVRYRVRTRF GPDTPVPMSP REYRRAVLPA LDGRAAASGT 1150
TDAMAPGAPD FCEEEAHSHA ACARWGLGAP LRPVYVALGR EAVRAGPARW 1200
RGPRRDFCAR ALLEPDDDAP PLVLRGDDDG PGALPPAPPG IRWASATGRS 1250
GTVLAAAGAV EVLGAEAGLA TPPRREVVDW EGAWDEDDGG AFEGDGVL 1298
Length:1,298
Mass (Da):132,844
Last modified:August 1, 1988 - v1
Checksum:i4F32E04C95CA9344
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32286.1.
X14112 Genomic DNA. Translation: CAA32278.1.
X06461 Genomic DNA. Translation: CAA29763.1.
L00036 Genomic DNA. Translation: AAA96675.1.
L00036 Genomic DNA. Translation: AAA96688.1.
PIRiA23510. EDBE75.
RefSeqiNP_044662.1. NC_001806.1.
NP_044676.1. NC_001806.1.

Genome annotation databases

GeneIDi2703391.
2703392.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14112 Genomic DNA. Translation: CAA32286.1 .
X14112 Genomic DNA. Translation: CAA32278.1 .
X06461 Genomic DNA. Translation: CAA29763.1 .
L00036 Genomic DNA. Translation: AAA96675.1 .
L00036 Genomic DNA. Translation: AAA96688.1 .
PIRi A23510. EDBE75.
RefSeqi NP_044662.1. NC_001806.1.
NP_044676.1. NC_001806.1.

3D structure databases

ProteinModelPortali P08392.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08392. 3 interactions.
MINTi MINT-6732561.

Proteomic databases

PRIDEi P08392.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2703391.
2703392.

Family and domain databases

InterProi IPR005205. Herpes_ICP4_C.
IPR005206. Herpes_ICP4_N.
[Graphical view ]
Pfami PF03585. Herpes_ICP4_C. 1 hit.
PF03584. Herpes_ICP4_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dolan A., Donald S., Brauer D.H.K.
    Nucleic Acids Res. 14:1727-1745(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1."
    McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., McNab D., Perry L.J., Scott J.E., Taylor P.
    J. Gen. Virol. 69:1531-1574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Codons 262 to 490 from the herpes simplex virus ICP4 gene are sufficient to encode a sequence-specific DNA binding protein."
    Wu C.-L., Wilcox K.W.
    Nucleic Acids Res. 18:531-538(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING DOMAIN.
  4. "The interaction of ICP4 with cell/infected-cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA."
    Papavassiliou A.G., Wilcox K.W., Silverstein S.J.
    EMBO J. 10:397-406(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  5. "Physical and functional interactions between herpes simplex virus immediate-early proteins ICP4 and ICP27."
    Panagiotidis C.A., Lium E.K., Silverstein S.J.
    J. Virol. 71:1547-1557(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ICP27.
  6. "Herpes simplex virus type 1 immediate-early protein ICP27 is required for efficient incorporation of ICP0 and ICP4 into virions."
    Sedlackova L., Rice S.A.
    J. Virol. 82:268-277(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiICP4_HHV11
AccessioniPrimary (citable) accession number: P08392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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