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Protein

UDP-N-acetylenolpyruvoylglucosamine reductase

Gene

murB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation.

Catalytic activityi

UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH.

Cofactori

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1591
Binding sitei190Substrate1
Active sitei229Proton donor1
Active sitei3251

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: EcoliWiki
  • UDP-N-acetylmuramate dehydrogenase activity Source: EcoCyc

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell wall organization Source: EcoliWiki
  • peptidoglycan biosynthetic process Source: EcoliWiki
  • regulation of cell shape Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciEcoCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER.
ECOL316407:JW3940-MONOMER.
MetaCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER.
SABIO-RKP08373.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase (EC:1.3.1.98)
Alternative name(s):
UDP-N-acetylmuramate dehydrogenase
Gene namesi
Name:murB
Synonyms:yijB
Ordered Locus Names:b3972, JW3940
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11205. murB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5526.
DrugBankiDB03147. Flavin adenine dinucleotide.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001792071 – 342UDP-N-acetylenolpyruvoylglucosamine reductaseAdd BLAST342

Proteomic databases

PaxDbiP08373.
PRIDEiP08373.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262982. 592 interactors.
DIPiDIP-10277N.
IntActiP08373. 12 interactors.
MINTiMINT-1275566.
STRINGi511145.b3972.

Chemistry databases

BindingDBiP08373.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi16 – 25Combined sources10
Helixi26 – 38Combined sources13
Beta strandi43 – 48Combined sources6
Beta strandi52 – 54Combined sources3
Beta strandi58 – 65Combined sources8
Beta strandi70 – 74Combined sources5
Beta strandi76 – 84Combined sources9
Helixi89 – 98Combined sources10
Helixi105 – 107Combined sources3
Helixi114 – 116Combined sources3
Turni117 – 121Combined sources5
Helixi129 – 131Combined sources3
Beta strandi133 – 140Combined sources8
Turni141 – 143Combined sources3
Beta strandi146 – 150Combined sources5
Helixi152 – 154Combined sources3
Helixi162 – 164Combined sources3
Turni165 – 170Combined sources6
Beta strandi171 – 183Combined sources13
Helixi191 – 195Combined sources5
Turni198 – 200Combined sources3
Helixi203 – 217Combined sources15
Turni221 – 223Combined sources3
Beta strandi226 – 231Combined sources6
Helixi238 – 247Combined sources10
Beta strandi253 – 255Combined sources3
Beta strandi261 – 263Combined sources3
Helixi265 – 271Combined sources7
Beta strandi282 – 284Combined sources3
Beta strandi286 – 288Combined sources3
Beta strandi291 – 294Combined sources4
Helixi300 – 318Combined sources19
Beta strandi326 – 330Combined sources5
Beta strandi333 – 335Combined sources3
Helixi337 – 341Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MBBX-ray2.30A1-342[»]
1MBTX-ray3.00A1-342[»]
1UXYX-ray1.80A3-342[»]
2MBRX-ray1.80A3-342[»]
2Q85X-ray2.51A1-342[»]
ProteinModelPortaliP08373.
SMRiP08373.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08373.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 183FAD-binding PCMH-typeAdd BLAST171

Sequence similaritiesi

Belongs to the MurB family.Curated
Contains 1 FAD-binding PCMH-type domain.Curated

Phylogenomic databases

eggNOGiENOG4105D4A. Bacteria.
COG0812. LUCA.
HOGENOMiHOG000284356.
InParanoidiP08373.
KOiK00075.
OMAiMQNIGAY.
PhylomeDBiP08373.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHSLKPWNT FGIDHNAQHI VCAEDEQQLL NAWQYATAEG QPVLILGEGS
60 70 80 90 100
NVLFLEDYRG TVIINRIKGI EIHDEPDAWY LHVGAGENWH RLVKYTLQEG
110 120 130 140 150
MPGLENLALI PGCVGSSPIQ NIGAYGVELQ RVCAYVDSVE LATGKQVRLT
160 170 180 190 200
AKECRFGYRD SIFKHEYQDR FAIVAVGLRL PKEWQPVLTY GDLTRLDPTT
210 220 230 240 250
VTPQQVFNAV CHMRTTKLPD PKVNGNAGSF FKNPVVSAET AKALLSQFPT
260 270 280 290 300
APNYPQADGS VKLAAGWLID QCQLKGMQIG GAAVHRQQAL VLINEDNAKS
310 320 330 340
EDVVQLAHHV RQKVGEKFNV WLEPEVRFIG ASGEVSAVET IS
Length:342
Mass (Da):37,851
Last modified:August 1, 1988 - v1
Checksum:iF43CFE29251E45C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35Y → H in AAA24185 (PubMed:1311302).Curated1
Sequence conflicti138S → C in AAA24185 (PubMed:1311302).Curated1
Sequence conflicti242K → E in AAA24185 (PubMed:1311302).Curated1
Sequence conflicti279I → M in AAA24185 (PubMed:1311302).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10123 Genomic DNA. Translation: AAA23519.1.
L14557 Genomic DNA. Translation: AAA24185.1.
U00006 Genomic DNA. Translation: AAC43074.1.
U00096 Genomic DNA. Translation: AAC76950.1.
AP009048 Genomic DNA. Translation: BAE77343.1.
V00348 Genomic DNA. No translation available.
PIRiA24029. QQECB8.
RefSeqiNP_418403.1. NC_000913.3.
WP_001016699.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76950; AAC76950; b3972.
BAE77343; BAE77343; BAE77343.
GeneIDi948470.
KEGGiecj:JW3940.
eco:b3972.
PATRICi32123465. VBIEscCol129921_4089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10123 Genomic DNA. Translation: AAA23519.1.
L14557 Genomic DNA. Translation: AAA24185.1.
U00006 Genomic DNA. Translation: AAC43074.1.
U00096 Genomic DNA. Translation: AAC76950.1.
AP009048 Genomic DNA. Translation: BAE77343.1.
V00348 Genomic DNA. No translation available.
PIRiA24029. QQECB8.
RefSeqiNP_418403.1. NC_000913.3.
WP_001016699.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MBBX-ray2.30A1-342[»]
1MBTX-ray3.00A1-342[»]
1UXYX-ray1.80A3-342[»]
2MBRX-ray1.80A3-342[»]
2Q85X-ray2.51A1-342[»]
ProteinModelPortaliP08373.
SMRiP08373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262982. 592 interactors.
DIPiDIP-10277N.
IntActiP08373. 12 interactors.
MINTiMINT-1275566.
STRINGi511145.b3972.

Chemistry databases

BindingDBiP08373.
ChEMBLiCHEMBL5526.
DrugBankiDB03147. Flavin adenine dinucleotide.

Proteomic databases

PaxDbiP08373.
PRIDEiP08373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76950; AAC76950; b3972.
BAE77343; BAE77343; BAE77343.
GeneIDi948470.
KEGGiecj:JW3940.
eco:b3972.
PATRICi32123465. VBIEscCol129921_4089.

Organism-specific databases

EchoBASEiEB1190.
EcoGeneiEG11205. murB.

Phylogenomic databases

eggNOGiENOG4105D4A. Bacteria.
COG0812. LUCA.
HOGENOMiHOG000284356.
InParanoidiP08373.
KOiK00075.
OMAiMQNIGAY.
PhylomeDBiP08373.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER.
ECOL316407:JW3940-MONOMER.
MetaCyc:UDPNACETYLMURAMATEDEHYDROG-MONOMER.
SABIO-RKP08373.

Miscellaneous databases

EvolutionaryTraceiP08373.
PROiP08373.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMURB_ECOLI
AccessioniPrimary (citable) accession number: P08373
Secondary accession number(s): Q2M8R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 2, 2016
This is version 167 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.