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Protein

Antitoxin ChpS

Gene

chpS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Antitoxin component of a type II toxin-antitoxin (TA) module. May be involved in the regulation of cell growth. It acts as a suppressor of the endoribonuclease (inhibitory function) of ChpB protein. Both ChpS and ChpB probably bind to the promoter region of the chpS-chpB operon to autoregulate their synthesis.2 Publications

GO - Molecular functioni

GO - Biological processi

  • positive regulation of cell growth Source: EcoCyc
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11250-MONOMER.
ECOL316407:JW5750-MONOMER.
MetaCyc:EG11250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Antitoxin ChpS
Gene namesi
Name:chpS
Synonyms:chpBI, yjfB
Ordered Locus Names:b4224, JW5750
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11250. chpS.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8383Antitoxin ChpSPRO_0000089651Add
BLAST

Proteomic databases

PaxDbiP08365.

Expressioni

Inductioni

Part of the chpS-chpB operon.1 Publication

Interactioni

Subunit structurei

Interacts with ChpB, inhibiting its endoribonuclease activity.1 Publication

Protein-protein interaction databases

DIPiDIP-9280N.
IntActiP08365. 1 interaction.
MINTiMINT-1263242.
STRINGi511145.b4224.

Structurei

3D structure databases

ProteinModelPortaliP08365.
SMRiP08365. Positions 1-74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 4846SpoVT-AbrBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PemI family.Curated
Contains 1 SpoVT-AbrB domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41087RY. Bacteria.
ENOG41102J6. LUCA.
HOGENOMiHOG000289070.
KOiK18842.
OMAiLEQMHAS.
OrthoDBiEOG61CM4D.
PhylomeDBiP08365.

Family and domain databases

InterProiIPR007159. SpoVT-AbrB_dom.
[Graphical view]
PfamiPF04014. MazE_antitoxin. 1 hit.
[Graphical view]
SMARTiSM00966. SpoVT_AbrB. 1 hit.
[Graphical view]
PROSITEiPS51740. SPOVT_ABRB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08365-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRITIKRWGN SAGMVIPNIV MKELNLQPGQ SVEAQVSNNQ LILTPISRRY
60 70 80
SLDELLAQCD MNAAELSEQD VWGKSTPAGD EIW
Length:83
Mass (Da):9,272
Last modified:August 1, 1988 - v1
Checksum:iA680B3BD47985A2D
GO

Sequence cautioni

The sequence AAA24011.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA97121.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16451 Genomic DNA. Translation: BAA41179.1.
M14018 Genomic DNA. Translation: AAA24011.1. Different initiation.
U14003 Genomic DNA. Translation: AAA97121.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77181.2.
AP009048 Genomic DNA. Translation: BAE78225.1.
PIRiS56450. QQECR8.
RefSeqiNP_418645.2. NC_000913.3.
WP_001223208.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77181; AAC77181; b4224.
BAE78225; BAE78225; BAE78225.
GeneIDi948739.
KEGGiecj:JW5750.
eco:b4224.
PATRICi32124023. VBIEscCol129921_4356.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16451 Genomic DNA. Translation: BAA41179.1.
M14018 Genomic DNA. Translation: AAA24011.1. Different initiation.
U14003 Genomic DNA. Translation: AAA97121.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77181.2.
AP009048 Genomic DNA. Translation: BAE78225.1.
PIRiS56450. QQECR8.
RefSeqiNP_418645.2. NC_000913.3.
WP_001223208.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP08365.
SMRiP08365. Positions 1-74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9280N.
IntActiP08365. 1 interaction.
MINTiMINT-1263242.
STRINGi511145.b4224.

Proteomic databases

PaxDbiP08365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77181; AAC77181; b4224.
BAE78225; BAE78225; BAE78225.
GeneIDi948739.
KEGGiecj:JW5750.
eco:b4224.
PATRICi32124023. VBIEscCol129921_4356.

Organism-specific databases

EchoBASEiEB1230.
EcoGeneiEG11250. chpS.

Phylogenomic databases

eggNOGiENOG41087RY. Bacteria.
ENOG41102J6. LUCA.
HOGENOMiHOG000289070.
KOiK18842.
OMAiLEQMHAS.
OrthoDBiEOG61CM4D.
PhylomeDBiP08365.

Enzyme and pathway databases

BioCyciEcoCyc:EG11250-MONOMER.
ECOL316407:JW5750-MONOMER.
MetaCyc:EG11250-MONOMER.

Miscellaneous databases

PROiP08365.

Family and domain databases

InterProiIPR007159. SpoVT-AbrB_dom.
[Graphical view]
PfamiPF04014. MazE_antitoxin. 1 hit.
[Graphical view]
SMARTiSM00966. SpoVT_AbrB. 1 hit.
[Graphical view]
PROSITEiPS51740. SPOVT_ABRB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100."
    Masuda Y., Miyakawa K., Nishimura Y., Ohtsubo E.
    J. Bacteriol. 175:6850-6856(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PROBABLE OPERON STRUCTURE.
    Strain: K12 / MC1000 / ATCC 39531.
  2. "Structural analysis of the ileR locus of Escherichia coli K12."
    Weiss D.L., Johnson D.I., Weith H.L., Somerville R.L.
    J. Biol. Chem. 261:9966-9971(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Mapping and disruption of the chpB locus in Escherichia coli."
    Masuda Y., Ohtsubo E.
    J. Bacteriol. 176:5861-5863(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE MAPPING, DISRUPTION PHENOTYPE.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Model for RNA binding and the catalytic site of the RNase Kid of the bacterial parD toxin-antitoxin system."
    Kamphuis M.B., Bonvin A.M., Monti M.C., Lemonnier M., Munoz-Gomez A., van den Heuvel R.H., Diaz-Orejas R., Boelens R.
    J. Mol. Biol. 357:115-126(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CHPB.

Entry informationi

Entry nameiCHPS_ECOLI
AccessioniPrimary (citable) accession number: P08365
Secondary accession number(s): P76803, Q2M681
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 11, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.