Reviewed, UniProtKB/Swiss-Prot P08365 (CHPS_ECOLI)

Names and origin

Protein names

Recommended name:
    PemI-like protein 2

Gene names

Name:	chpS
Synonyms:	chpBI, yjfB
Ordered Locus Names:	b4224, JW5750

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	83 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the regulation of cell growth. It acts as a suppressor of the inhibitory function of chpB protein. Both chpS and chpB bind to the promoter region of the chpSB operon to autoregulate their synthesis.
Sequence similarities	Belongs to the pemI family.

Ontologies

Keywords
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pta	P0A9M8	1	EBI-556499,EBI-555015

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 83	83	PemI-like protein 2		PRO_0000089651

Sequences

	Sequence		Length	Mass (Da)
	P08365-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: A680B3BD47985A2D	FASTA	83	9,272
	10 20 30 40 50 60 MRITIKRWGN SAGMVIPNIV MKELNLQPGQ SVEAQVSNNQ LILTPISRRY SLDELLAQCD 70 80 MNAAELSEQD VWGKSTPAGD EIW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"chpA and chpB, Escherichia coli chromosomal homologs of the pem locus responsible for stable maintenance of plasmid R100." Masuda Y., Miyakawa K., Nishimura Y., Ohtsubo E. J. Bacteriol. 175:6850-6856(1993) [PubMed: 8226627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Structural analysis of the ileR locus of Escherichia coli K12." Weiss D.L., Johnson D.I., Weith H.L., Somerville R.L. J. Biol. Chem. 261:9966-9971(1986) [PubMed: 3525538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Mapping and disruption of the chpB locus in Escherichia coli." Masuda Y., Ohtsubo E. J. Bacteriol. 176:5861-5863(1994) [PubMed: 8083180] [Abstract] Cited for: GENE MAPPING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D16451 Genomic DNA. Translation: BAA41179.1. M14018 Genomic DNA. Translation: AAA24011.1. Different initiation. U14003 Genomic DNA. Translation: AAA97121.1. Different initiation. U00096 Genomic DNA. Translation: AAC77181.1. Different initiation. AP009048 Genomic DNA. Translation: BAE78225.1.
PIR	QQECR8. S56450.
RefSeq	AP_004724.1. NP_418645.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9280N.
IntAct	P08365. 1 interaction.
STRING	P08365.
Genome annotation databases
GeneID	948739.
GenomeReviews	Gene locus JW5750 in contig AP009048_GR. Gene locus b4224 in contig U00096_GR.
KEGG	ecj:JW5750. eco:b4224.
Organism-specific databases
EchoBASE	EB1230.
EcoGene	EG11250. chpS.
CMR	Search...
Phylogenomic databases
HOGENOM	P08365.
OMA	QQWGNSA
Enzyme and pathway databases
BioCyc	EcoCyc:EG11250-MON. ECOL168927:B4224-MON.
Gene expression databases
Genevestigator	P08365.
Family and domain databases
InterPro	IPR007159. Tscrpt_reg_AbrB/SpoVT. [Graphical view]
Pfam	PF04014. SpoVT_AbrB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHPS_ECOLI

Accession

Primary (citable) accession number: P08365
Secondary accession number(s): P76803, Q2M681

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	November 24, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)