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P08360

- ENV_MLVCB

UniProt

P08360 - ENV_MLVCB

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Protein
Envelope glycoprotein
Gene
env
Organism
Cas-Br-E murine leukemia virus
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The surface protein (SU) attaches the virus to the host cell by binding to its receptor. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. Fusion occurs at the host cell plasma membrane By similarity.
The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861Zinc By similarity
Metal bindingi117 – 1171Zinc By similarity
Sitei465 – 4662Cleavage; by host By similarity
Sitei645 – 6462Cleavage; by viral protease p14

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Alternative name(s):
Env polyprotein
Cleaved into the following 3 chains:
Surface protein
Short name:
SU
Alternative name(s):
Glycoprotein 70
Short name:
gp70
Transmembrane protein
Short name:
TM
Alternative name(s):
Envelope protein p15E
Alternative name(s):
p2E
Gene namesi
Name:env
OrganismiCas-Br-E murine leukemia virus
Taxonomic identifieri11792 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

Chain Surface protein : Virion membrane; Peripheral membrane protein. Host cell membrane; Peripheral membrane protein By similarity
Note: The surface protein is not anchored to the viral envelope, but associates with the virion surface through its binding to TM. Both proteins are thought to be concentrated at the site of budding and incorporated into the virions possibly by contacts between the cytoplasmic tail of Env and the N-terminus of Gag By similarity.
R-peptide : Host cell membrane; Peripheral membrane protein By similarity
Note: The R-peptide is membrane-associated through its palmitate By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 606573Extracellular Reviewed prediction
Add
BLAST
Transmembranei607 – 62721Helical; Reviewed prediction
Add
BLAST
Topological domaini628 – 66134Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral capsid Source: InterPro
  4. viral envelope Source: UniProtKB-KW
  5. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333 Reviewed prediction
Add
BLAST
Chaini34 – 661628Envelope glycoprotein
PRO_0000239580Add
BLAST
Chaini34 – 465432Surface protein By similarity
PRO_0000040748Add
BLAST
Chaini466 – 645180Transmembrane protein By similarity
PRO_0000040749Add
BLAST
Peptidei646 – 66116R-peptide
PRO_0000040750Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...); by host By similarity
Disulfide bondi77 ↔ 129 By similarity
Disulfide bondi103 ↔ 118 By similarity
Disulfide bondi104 ↔ 114 By similarity
Disulfide bondi152 ↔ 172 By similarity
Disulfide bondi164 ↔ 177 By similarity
Glycosylationi199 – 1991N-linked (GlcNAc...); by host By similarity
Disulfide bondi209 ↔ 215 By similarity
Glycosylationi322 – 3221N-linked (GlcNAc...); by host By similarity
Disulfide bondi332 ↔ 559Interchain (between SU and TM chains, or C-335 with C-559); in linked form
Disulfide bondi332 ↔ 335
Glycosylationi361 – 3611N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi362 ↔ 416 By similarity
Disulfide bondi381 ↔ 393 By similarity
Glycosylationi394 – 3941N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi423 ↔ 436 By similarity
Glycosylationi430 – 4301N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi551 ↔ 558 By similarity
Lipidationi626 – 6261S-palmitoyl cysteine; by host By similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. Envelope glycoproteins are synthesized as a inactive precursor that is N-glycosylated and processed likely by host cell furin or by a furin-like protease in the Golgi to yield the mature SU and TM proteins. The cleavage site between SU and TM requires the minimal sequence [KR]-X-[KR]-R. The R-peptide is released from the C-terminus of the cytoplasmic tail of the TM protein upon particle formation as a result of proteolytic cleavage by the viral protease. Cleavage of this peptide is required for TM to become fusogenic By similarity.
The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion By similarity.
The transmembrane protein is palmitoylated By similarity.
The R-peptide is palmitoylated By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

The mature envelope protein (Env) consists of a trimer of SU-TM heterodimers attached by a labile interchain disulfide bond By similarity.

Structurei

3D structure databases

ProteinModelPortaliP08360.
SMRiP08360. Positions 40-266, 511-563.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni32 – 267236Receptor-binding domain (RBD) Reviewed prediction
Add
BLAST
Regioni468 – 48821Fusion peptide By similarity
Add
BLAST
Regioni534 – 55017Immunosuppression By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili497 – 53337 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi332 – 3354CXXC
Motifi551 – 5599CX6CC
Motifi651 – 6544YXXL motif; contains endocytosis signal By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi264 – 30239Pro-rich
Add
BLAST

Domaini

The YXXL motif is involved in determining the exact site of viral release at the surface of infected mononuclear cells and promotes endocytosis.
The 17 amino acids long immunosuppressive region is present in many retroviral envelope proteins. Synthetic peptides derived from this relatively conserved sequence inhibit immune function in vitro and in vivo By similarity.

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.310.10. 1 hit.
InterProiIPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view]
PANTHERiPTHR10424. PTHR10424. 1 hit.
PfamiPF00429. TLV_coat. 1 hit.
[Graphical view]
SUPFAMiSSF49830. SSF49830. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08360-1 [UniParc]FASTAAdd to Basket

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MEGPAFSKSP KDKTIERAFL GVLGILFVTG GLASRDNPHQ VYNITWEVTN    50
GEQDTVWAVT GNHPLWTWWP DLTPDLCMLA LHGPTHWGLD NHPPYSSPPG 100
PPCCSGDAGA VSGCARDCDE PLTSYSPRCN TAWNRLKLAR VTHAPKEGFY 150
ICPGSHRPRW ARSCGGLDAY YCASWGCETT GRAAWNPTSS WDYITVSNNL 200
TSSQATKACK NNGWCNPLVI RFTGPGKRAT SWTTGHFWGL RLYISGHDPG 250
LTFGIRLKVT DLGPRVPIGP NPVLSDQRPP SRPVPARPPP PSASPSTPTI 300
PPQQGTGDRL LNLVQGAYLT LNMTDPTRTQ ECWLCLVSEP PYYEGVAVLR 350
EYTSHETAPA NCSSGSQHKL TLSEVTGQGR CLGTVPKTHQ ALCNRTEPTV 400
SGSNYLVAPE GTLWACSTGL TPCLSTTVLN LTTDYCVLVE LWPKVTYHSP 450
DYVYTQFEPG ARFRREPVSL TLALLPEGLT MGGIAAGVGT GTTALVATQQ 500
FQQLQAAMHN DLKEVEKSIT NLEKSLTSLS EVVLQNRRGL DLLFLKEGGL 550
CAALKEECCF YADHTGLVRD SMAKLRERLN QRQKLFESGQ GWFEGLFNRS 600
PWFTTLISTI MGPLIVLLLI LLFGPCILNR LVQFVKDRIS VVQALVLTQQ 650
YHQLKPIEYE P 661
Length:661
Mass (Da):72,625
Last modified:August 1, 1988 - v1
Checksum:i255BEE3AF62FB9FF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti476 – 4772PE → LG1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14702 Genomic DNA. Translation: AAA46512.1.
X57540 Genomic DNA. No translation available.
PIRiB26103. VCMVCB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14702 Genomic DNA. Translation: AAA46512.1 .
X57540 Genomic DNA. No translation available.
PIRi B26103. VCMVCB.

3D structure databases

ProteinModelPortali P08360.
SMRi P08360. Positions 40-266, 511-563.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.310.10. 1 hit.
InterProi IPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view ]
PANTHERi PTHR10424. PTHR10424. 1 hit.
Pfami PF00429. TLV_coat. 1 hit.
[Graphical view ]
SUPFAMi SSF49830. SSF49830. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cas-Br-E murine leukemia virus: sequencing of the paralytogenic region of its genome and derivation of specific probes to study its origin and the structure of its recombinant genomes in leukemic tissues."
    Rassart E., Nelbach L., Jolicoeur P.
    J. Virol. 60:910-919(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of the neurotropic murine retrovirus CAS-BR-E."
    Perryman S.M., McAtee F.J., Portis J.L.
    Nucleic Acids Res. 19:1707-1707(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Identification of R-peptides in envelope proteins of C-type retroviruses."
    Bobkova M., Stitz J., Engelstadter M., Cichutek K., Buchholz C.J.
    J. Gen. Virol. 83:2241-2246(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF R-PEPTIDE.

Entry informationi

Entry nameiENV_MLVCB
AccessioniPrimary (citable) accession number: P08360
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

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