Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Muconate cycloisomerase 1

Gene

catB

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a syn cycloisomerization.

Catalytic activityi

+-muconolactone = cis,cis-muconate.1 Publication

Cofactori

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from catechol.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Muconate cycloisomerase 1 (catB)
  3. Muconolactone Delta-isomerase (AYO28_17920), Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase (HA62_11725), Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase (A3L25_20555), Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase, Muconolactone Delta-isomerase (catC), Muconolactone Delta-isomerase (HB4184_27310), Muconolactone Delta-isomerase (PU99_13590), Muconolactone Delta-isomerase
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from catechol, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Proton acceptor1
Metal bindingi200Manganese1
Metal bindingi226Manganese1
Metal bindingi251Manganese1
Active sitei329Proton donor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3423.
BRENDAi5.5.1.1. 5092.
SABIO-RKP08310.
UniPathwayiUPA00157; UER00259.

Names & Taxonomyi

Protein namesi
Recommended name:
Muconate cycloisomerase 1 (EC:5.5.1.11 Publication)
Alternative name(s):
Cis,cis-muconate lactonizing enzyme I
Short name:
MLE
Muconate cycloisomerase I
Gene namesi
Name:catB
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001712512 – 375Muconate cycloisomerase 1Add BLAST374

Proteomic databases

PRIDEiP08310.

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

STRINGi160488.PP_3715.

Structurei

Secondary structure

1375
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 14Combined sources10
Beta strandi16 – 19Combined sources4
Beta strandi33 – 43Combined sources11
Beta strandi48 – 54Combined sources7
Turni57 – 61Combined sources5
Helixi66 – 75Combined sources10
Helixi77 – 81Combined sources5
Helixi89 – 99Combined sources11
Helixi104 – 122Combined sources19
Helixi126 – 129Combined sources4
Beta strandi136 – 140Combined sources5
Beta strandi142 – 144Combined sources3
Helixi149 – 161Combined sources13
Beta strandi166 – 171Combined sources6
Beta strandi173 – 175Combined sources3
Helixi177 – 191Combined sources15
Helixi192 – 194Combined sources3
Beta strandi195 – 200Combined sources6
Helixi207 – 219Combined sources13
Helixi234 – 243Combined sources10
Beta strandi248 – 251Combined sources4
Helixi257 – 266Combined sources10
Beta strandi270 – 274Combined sources5
Helixi276 – 279Combined sources4
Helixi282 – 295Combined sources14
Beta strandi298 – 301Combined sources4
Helixi308 – 318Combined sources11
Helixi332 – 335Combined sources4
Beta strandi336 – 338Combined sources3
Beta strandi340 – 343Combined sources4
Beta strandi346 – 348Combined sources3
Beta strandi351 – 353Combined sources3
Helixi366 – 372Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MUCX-ray1.85A/B1-375[»]
2MUCX-ray2.30A/B1-375[»]
3MUCX-ray2.30A/B5-374[»]
ProteinModelPortaliP08310.
SMRiP08310.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08310.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DTQ. Bacteria.
COG4948. LUCA.

Family and domain databases

CDDicd03318. MLE. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08310-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSVLIERIE AIIVHDLPTI RPPHKLAMHT MQTQTLVLIR VRCSDGVEGI
60 70 80 90 100
GEATTIGGLA YGYESPEGIK ANIDAHLAPA LVGLPADNIN AAMLKLDKLA
110 120 130 140 150
KGNTFAKSGI ESALLDAQGK RLGLPVSELL GGRVRDSLEV AWTLASGDTA
160 170 180 190 200
RDIAEAQHML EIRRHRVFKL KIGANPLAQD LKHVVAIKRE LGDSASVRVD
210 220 230 240 250
VNQYWDESQA IRACQVLGDN GIDLIEQPIS RINRSGQVRL NQRSPAPIMA
260 270 280 290 300
DESIESVEDA FSLAADGAAS IFALKIAKNG GPRAVLRTAQ IAEAAGIALY
310 320 330 340 350
GGTMLEGSIG TLASAHAFLT LRQLTWGTEL FGPLLLTEEI VNEPPQYRDF
360 370
QLHIPRTPGL GLTLDEQRLA RFARR
Length:375
Mass (Da):40,510
Last modified:January 23, 2007 - v4
Checksum:i4C8668BF6765D061
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33T → S in AAA25766 (PubMed:2449420).Curated1
Sequence conflicti65S → T in AAA25766 (PubMed:2449420).Curated1
Sequence conflicti244Missing in AAA25765 (PubMed:3609743).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19460 Genomic DNA. Translation: AAA25766.1.
M16236 Genomic DNA. Translation: AAA25765.1.
PIRiA28630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19460 Genomic DNA. Translation: AAA25766.1.
M16236 Genomic DNA. Translation: AAA25765.1.
PIRiA28630.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MUCX-ray1.85A/B1-375[»]
2MUCX-ray2.30A/B1-375[»]
3MUCX-ray2.30A/B5-374[»]
ProteinModelPortaliP08310.
SMRiP08310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_3715.

Proteomic databases

PRIDEiP08310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105DTQ. Bacteria.
COG4948. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00259.
BioCyciMetaCyc:MONOMER-3423.
BRENDAi5.5.1.1. 5092.
SABIO-RKP08310.

Miscellaneous databases

EvolutionaryTraceiP08310.

Family and domain databases

CDDicd03318. MLE. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCATB_PSEPU
AccessioniPrimary (citable) accession number: P08310
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 116 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.