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P08306

- COX2_PARDE

UniProt

P08306 - COX2_PARDE

Protein

Cytochrome c oxidase subunit 2

Gene

ctaC

Organism
Paracoccus denitrificans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Cofactori

    Binds 2 copper A ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi210 – 2101Copper A1
    Metal bindingi245 – 2451Copper A1
    Metal bindingi245 – 2451Copper A2
    Metal bindingi247 – 2471Copper A2; via carbonyl oxygen
    Metal bindingi249 – 2491Copper A1
    Metal bindingi249 – 2491Copper A2
    Metal bindingi253 – 2531Copper A2
    Metal bindingi256 – 2561Copper A1

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. cytochrome-c oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. electron transport chain Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Metal-binding

    Protein family/group databases

    TCDBi3.D.4.6.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 2 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome aa3 subunit 2
    Cytochrome c oxidase polypeptide II
    Oxidase aa(3) subunit 2
    Gene namesi
    Name:ctaC
    Synonyms:coiI, ctaB
    OrganismiParacoccus denitrificans
    Taxonomic identifieri266 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Add
    BLAST
    Chaini30 – 280251Cytochrome c oxidase subunit 2PRO_0000006059Add
    BLAST
    Propeptidei281 – 29818C-terminal propeptidePRO_0000006060Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301Pyrrolidone carboxylic acid

    Keywords - PTMi

    Pyrrolidone carboxylic acid

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-6089N.

    Structurei

    Secondary structure

    1
    298
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni32 – 354
    Beta strandi38 – 403
    Helixi55 – 8834
    Turni91 – 933
    Beta strandi94 – 963
    Helixi104 – 13330
    Beta strandi140 – 1489
    Beta strandi151 – 1566
    Turni157 – 1604
    Beta strandi161 – 1655
    Helixi170 – 1723
    Helixi174 – 1763
    Helixi180 – 1823
    Turni183 – 1853
    Beta strandi187 – 1893
    Beta strandi191 – 20818
    Beta strandi210 – 2145
    Helixi215 – 2173
    Beta strandi219 – 2235
    Beta strandi229 – 2346
    Beta strandi236 – 2438
    Turni251 – 2544
    Beta strandi258 – 2636
    Helixi265 – 27814

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AR1X-ray2.70B1-298[»]
    1QLEX-ray3.00B30-281[»]
    1ZYYmodel-B30-281[»]
    3EHBX-ray2.32B1-298[»]
    3HB3X-ray2.25B1-298[»]
    ProteinModelPortaliP08306.
    SMRiP08306. Positions 30-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08306.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 5526PeriplasmicAdd
    BLAST
    Topological domaini89 – 10315CytoplasmicAdd
    BLAST
    Topological domaini135 – 280146PeriplasmicAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei56 – 8833HelicalAdd
    BLAST
    Transmembranei104 – 13431HelicalAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.287.90. 1 hit.
    2.60.40.420. 1 hit.
    InterProiIPR001505. Copper_CuA.
    IPR008972. Cupredoxin.
    IPR014222. Cyt_c_oxidase_su2.
    IPR002429. Cyt_c_oxidase_su2_C.
    IPR011759. Cyt_c_oxidase_su2_TM_dom.
    [Graphical view]
    PfamiPF00116. COX2. 1 hit.
    PF02790. COX2_TM. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 1 hit.
    SSF81464. SSF81464. 1 hit.
    TIGRFAMsiTIGR02866. CoxB. 1 hit.
    PROSITEiPS00078. COX2. 1 hit.
    PS50857. COX2_CUA. 1 hit.
    PS50999. COX2_TM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08306-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMAIATKRRG VAAVMSLGVA TMTAVPALAQ DVLGDLPVIG KPVNGGMNFQ    50
    PASSPLAHDQ QWLDHFVLYI ITAVTIFVCL LLLICIVRFN RRANPVPARF 100
    THNTPIEVIW TLVPVLILVA IGAFSLPILF RSQEMPNDPD LVIKAIGHQW 150
    YWSYEYPNDG VAFDALMLEK EALADAGYSE DEYLLATDNP VVVPVGKKVL 200
    VQVTATDVIH AWTIPAFAVK QDAVPGRIAQ LWFSVDQEGV YFGQCSELCG 250
    INHAYMPIVV KAVSQEKYEA WLAGAKEEFA ADASDYLPAS PVKLASAE 298
    Length:298
    Mass (Da):32,539
    Last modified:August 1, 1988 - v1
    Checksum:i918A64A9E3B93366
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti160 – 1612GV → AF AA sequence (PubMed:2820725)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05828 Genomic DNA. Translation: CAA29268.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05828 Genomic DNA. Translation: CAA29268.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AR1 X-ray 2.70 B 1-298 [» ]
    1QLE X-ray 3.00 B 30-281 [» ]
    1ZYY model - B 30-281 [» ]
    3EHB X-ray 2.32 B 1-298 [» ]
    3HB3 X-ray 2.25 B 1-298 [» ]
    ProteinModelPortali P08306.
    SMRi P08306. Positions 30-281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6089N.

    Protein family/group databases

    TCDBi 3.D.4.6.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P08306.

    Family and domain databases

    Gene3Di 1.10.287.90. 1 hit.
    2.60.40.420. 1 hit.
    InterProi IPR001505. Copper_CuA.
    IPR008972. Cupredoxin.
    IPR014222. Cyt_c_oxidase_su2.
    IPR002429. Cyt_c_oxidase_su2_C.
    IPR011759. Cyt_c_oxidase_su2_TM_dom.
    [Graphical view ]
    Pfami PF00116. COX2. 1 hit.
    PF02790. COX2_TM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 1 hit.
    SSF81464. SSF81464. 1 hit.
    TIGRFAMsi TIGR02866. CoxB. 1 hit.
    PROSITEi PS00078. COX2. 1 hit.
    PS50857. COX2_CUA. 1 hit.
    PS50999. COX2_TM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and analysis of the genes for cytochrome c oxidase in Paracoccus denitrificans."
      Raitio M., Jalli T., Saraste M.
      EMBO J. 6:2825-2833(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S1657.
    2. "Subunit II of cytochrome c oxidase from Paracoccus denitrificans. DNA sequence, gene expression and the protein."
      Steinruecke P., Steffens G.C.M., Panskus G., Buse G., Ludwig B.
      Eur. J. Biochem. 167:431-439(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 13543 / NRRL B-3784 / NRC 449.
    3. "Structure at 2.8-A resolution of cytochrome c oxidase from Paracoccus denitrificans."
      Iwata S., Ostermeier C., Ludwig B., Michel H.
      Nature 376:660-669(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    4. "Structure at 2.7-A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment."
      Ostermeier C., Harrenga A., Ermler U., Michel H.
      Proc. Natl. Acad. Sci. U.S.A. 94:10547-10553(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

    Entry informationi

    Entry nameiCOX2_PARDE
    AccessioniPrimary (citable) accession number: P08306
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3