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Protein

Cytochrome c oxidase subunit 2

Gene

ctaC

Organism
Paracoccus denitrificans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactori

binuclear copper center (CuA)Note: Binds a binuclear copper A center per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi210 – 2101Copper A1
Metal bindingi245 – 2451Copper A1
Metal bindingi245 – 2451Copper A2
Metal bindingi247 – 2471Copper A2; via carbonyl oxygen
Metal bindingi249 – 2491Copper A1
Metal bindingi249 – 2491Copper A2
Metal bindingi253 – 2531Copper A2
Metal bindingi256 – 2561Copper A1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BRENDAi1.9.3.1. 3341.

Protein family/group databases

TCDBi3.D.4.6.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 2 (EC:1.9.3.1)
Alternative name(s):
Cytochrome aa3 subunit 2
Cytochrome c oxidase polypeptide II
Oxidase aa(3) subunit 2
Gene namesi
Name:ctaC
Synonyms:coiI, ctaB
OrganismiParacoccus denitrificans
Taxonomic identifieri266 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 5526PeriplasmicAdd
BLAST
Transmembranei56 – 8833HelicalAdd
BLAST
Topological domaini89 – 10315CytoplasmicAdd
BLAST
Transmembranei104 – 13431HelicalAdd
BLAST
Topological domaini135 – 280146PeriplasmicAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Add
BLAST
Chaini30 – 280251Cytochrome c oxidase subunit 2PRO_0000006059Add
BLAST
Propeptidei281 – 29818C-terminal propeptidePRO_0000006060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Pyrrolidone carboxylic acid

Keywords - PTMi

Pyrrolidone carboxylic acid

Interactioni

Protein-protein interaction databases

DIPiDIP-6089N.
STRINGi318586.Pden_4321.

Structurei

Secondary structure

1
298
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni32 – 354Combined sources
Beta strandi38 – 403Combined sources
Helixi55 – 8834Combined sources
Turni91 – 933Combined sources
Beta strandi94 – 963Combined sources
Helixi104 – 13330Combined sources
Beta strandi140 – 1489Combined sources
Beta strandi151 – 1566Combined sources
Turni157 – 1604Combined sources
Beta strandi161 – 1655Combined sources
Helixi170 – 1723Combined sources
Helixi174 – 1763Combined sources
Helixi180 – 1823Combined sources
Turni183 – 1853Combined sources
Beta strandi187 – 1893Combined sources
Beta strandi191 – 20818Combined sources
Beta strandi210 – 2145Combined sources
Helixi215 – 2173Combined sources
Beta strandi219 – 2235Combined sources
Beta strandi229 – 2346Combined sources
Beta strandi236 – 2438Combined sources
Turni251 – 2544Combined sources
Beta strandi258 – 2636Combined sources
Helixi265 – 27814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AR1X-ray2.70B1-298[»]
1QLEX-ray3.00B30-281[»]
1ZYYmodel-B30-281[»]
3EHBX-ray2.32B1-298[»]
3HB3X-ray2.25B1-298[»]
ProteinModelPortaliP08306.
SMRiP08306. Positions 30-281.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08306.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAIATKRRG VAAVMSLGVA TMTAVPALAQ DVLGDLPVIG KPVNGGMNFQ
60 70 80 90 100
PASSPLAHDQ QWLDHFVLYI ITAVTIFVCL LLLICIVRFN RRANPVPARF
110 120 130 140 150
THNTPIEVIW TLVPVLILVA IGAFSLPILF RSQEMPNDPD LVIKAIGHQW
160 170 180 190 200
YWSYEYPNDG VAFDALMLEK EALADAGYSE DEYLLATDNP VVVPVGKKVL
210 220 230 240 250
VQVTATDVIH AWTIPAFAVK QDAVPGRIAQ LWFSVDQEGV YFGQCSELCG
260 270 280 290
INHAYMPIVV KAVSQEKYEA WLAGAKEEFA ADASDYLPAS PVKLASAE
Length:298
Mass (Da):32,539
Last modified:August 1, 1988 - v1
Checksum:i918A64A9E3B93366
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1612GV → AF AA sequence (PubMed:2820725).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05828 Genomic DNA. Translation: CAA29268.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05828 Genomic DNA. Translation: CAA29268.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AR1X-ray2.70B1-298[»]
1QLEX-ray3.00B30-281[»]
1ZYYmodel-B30-281[»]
3EHBX-ray2.32B1-298[»]
3HB3X-ray2.25B1-298[»]
ProteinModelPortaliP08306.
SMRiP08306. Positions 30-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6089N.
STRINGi318586.Pden_4321.

Protein family/group databases

TCDBi3.D.4.6.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.9.3.1. 3341.

Miscellaneous databases

EvolutionaryTraceiP08306.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and analysis of the genes for cytochrome c oxidase in Paracoccus denitrificans."
    Raitio M., Jalli T., Saraste M.
    EMBO J. 6:2825-2833(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S1657.
  2. "Subunit II of cytochrome c oxidase from Paracoccus denitrificans. DNA sequence, gene expression and the protein."
    Steinruecke P., Steffens G.C.M., Panskus G., Buse G., Ludwig B.
    Eur. J. Biochem. 167:431-439(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 13543 / NRRL B-3784 / NRC 449.
  3. "Structure at 2.8-A resolution of cytochrome c oxidase from Paracoccus denitrificans."
    Iwata S., Ostermeier C., Ludwig B., Michel H.
    Nature 376:660-669(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  4. "Structure at 2.7-A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment."
    Ostermeier C., Harrenga A., Ermler U., Michel H.
    Proc. Natl. Acad. Sci. U.S.A. 94:10547-10553(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).

Entry informationi

Entry nameiCOX2_PARDE
AccessioniPrimary (citable) accession number: P08306
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: June 24, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.