Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Asialoglycoprotein receptor 2

Gene

Asgr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Calcium, Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Asialoglycoprotein receptor 2
Short name:
ASGP-R 2
Short name:
ASGPR 2
Alternative name(s):
Hepatic lectin R2/3
Short name:
HL-2
Short name:
rHL-2
Gene namesi
Name:Asgr2
Synonyms:Asgr-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2161. Asgr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5858CytoplasmicSequence analysisAdd
BLAST
Transmembranei59 – 7921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini80 – 301222ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Asialoglycoprotein receptor 2PRO_0000046656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphoserineCombined sources
Lipidationi54 – 541S-palmitoyl cysteine1 Publication
Glycosylationi97 – 971N-linked (GlcNAc...)
Glycosylationi119 – 1191N-linked (GlcNAc...)
Glycosylationi165 – 1651N-linked (GlcNAc...)
Disulfide bondi170 ↔ 181PROSITE-ProRule annotation
Disulfide bondi198 ↔ 293PROSITE-ProRule annotation
Disulfide bondi271 ↔ 285PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP08290.
PRIDEiP08290.

PTM databases

iPTMnetiP08290.
PhosphoSiteiP08290.
SwissPalmiP08290.

Expressioni

Tissue specificityi

Expressed exclusively in hepatic parenchymal cells.

Gene expression databases

ExpressionAtlasiP08290. baseline and differential.
GenevisibleiP08290. RN.

Interactioni

Subunit structurei

Interacts with LASS2.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060474.

Structurei

3D structure databases

ProteinModelPortaliP08290.
SMRiP08290. Positions 170-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini169 – 295127C-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00760000118924.
HOGENOMiHOG000034093.
HOVERGENiHBG000270.
InParanoidiP08290.
KOiK10064.
OMAiQDLKADH.
PhylomeDBiP08290.
TreeFamiTF352155.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKDFQDIQQ LDSEENDHQL IGDEEQGSHV QNLRTENPRW GGQPPSRPFP
60 70 80 90 100
QRLCSKFRLS LLALAFNILL LVVICVVSSQ SMQLQKEFWT LKETLSNFST
110 120 130 140 150
TTLMEFKALD SHGGSRNDNL TSWETILEKK QKDIKADHST LLFHLKHFPL
160 170 180 190 200
DLRTLTCQLA FFLSNGTECC PVNWVEFGGS CYWFSRDGLT WAEADQYCQM
210 220 230 240 250
ENAHLLVINS REEQEFVVKH RGAFHIWIGL TDKDGSWKWV DGTEYRSNFK
260 270 280 290 300
NWAFTQPDNW QGHEEGGSED CAEILSDGLW NDNFCQQVNR WACERKRDIT

Y
Length:301
Mass (Da):35,027
Last modified:May 1, 2007 - v4
Checksum:i21832FA713C3433C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1531R → A in AAA42038 (PubMed:3600647).Curated
Sequence conflicti202 – 2021N → I in AAA42038 (PubMed:3600647).Curated
Sequence conflicti260 – 2601W → C in AAA42038 (PubMed:3600647).Curated
Sequence conflicti260 – 2601W → C AA sequence (PubMed:6319386).Curated
Sequence conflicti282 – 2832DN → ND AA sequence (PubMed:6319386).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16347 mRNA. Translation: AAA42038.1.
J02762 mRNA. Translation: AAA41522.1.
X07636 mRNA. Translation: CAA30476.1.
BC088099 mRNA. Translation: AAH88099.1.
PIRiB28462. LNRT2.
RefSeqiNP_058885.1. NM_017189.2.
XP_006246738.1. XM_006246676.2.
UniGeneiRn.9834.

Genome annotation databases

EnsembliENSRNOT00000065370; ENSRNOP00000060474; ENSRNOG00000030726.
GeneIDi29403.
KEGGirno:29403.
UCSCiRGD:2161. rat.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Hepatic asialoglycoprotein receptor subunit 2/3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16347 mRNA. Translation: AAA42038.1.
J02762 mRNA. Translation: AAA41522.1.
X07636 mRNA. Translation: CAA30476.1.
BC088099 mRNA. Translation: AAH88099.1.
PIRiB28462. LNRT2.
RefSeqiNP_058885.1. NM_017189.2.
XP_006246738.1. XM_006246676.2.
UniGeneiRn.9834.

3D structure databases

ProteinModelPortaliP08290.
SMRiP08290. Positions 170-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060474.

PTM databases

iPTMnetiP08290.
PhosphoSiteiP08290.
SwissPalmiP08290.

Proteomic databases

PaxDbiP08290.
PRIDEiP08290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000065370; ENSRNOP00000060474; ENSRNOG00000030726.
GeneIDi29403.
KEGGirno:29403.
UCSCiRGD:2161. rat.

Organism-specific databases

CTDi433.
RGDi2161. Asgr2.

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00760000118924.
HOGENOMiHOG000034093.
HOVERGENiHBG000270.
InParanoidiP08290.
KOiK10064.
OMAiQDLKADH.
PhylomeDBiP08290.
TreeFamiTF352155.

Miscellaneous databases

NextBioi609051.
PROiP08290.

Gene expression databases

ExpressionAtlasiP08290. baseline and differential.
GenevisibleiP08290. RN.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR005640. Lectin_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF03954. Lectin_N. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of cDNA clones encoding two homologous proteins that are part of the asialoglycoprotein receptor."
    McPhaul M., Berg P.
    Mol. Cell. Biol. 7:1841-1847(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Major and minor forms of the rat liver asialoglycoprotein receptor are independent galactose-binding proteins. Primary structure and glycosylation heterogeneity of minor receptor forms."
    Halberg D.F., Wager R.E., Farrell D.C., Hildreth J., Quesenberry M.S., Loeb J.A., Holland E.C., Drickamer K.
    J. Biol. Chem. 262:9828-9838(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Asialoglycoprotein receptor genes are linked on chromosome 11 in the mouse."
    Sanford J.P., Elliott R.W., Doyle D.
    DNA 7:721-728(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "Primary structure of the rat liver asialoglycoprotein receptor. Structural evidence for multiple polypeptide species."
    Drickamer K., Mamon J.F., Binns G., Leung J.O.
    J. Biol. Chem. 259:770-778(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 201-301.
  6. "Fatty acylation of the rat and human asialoglycoprotein receptors. A conserved cytoplasmic cysteine residue is acylated in all receptor subunits."
    Zeng F.Y., Weigel P.H.
    J. Biol. Chem. 271:32454-32460(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-54.
  7. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiASGR2_RAT
AccessioniPrimary (citable) accession number: P08290
Secondary accession number(s): Q5M8C9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 2007
Last modified: February 17, 2016
This is version 129 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Calcium is required for ligand binding.
Two types of rat hepatic lectin have been identified, RHL-1 and RHL-2/3, having a relative abundance of 4:1. RHL-2 and RHL-3 only differs in their carbohydrate structures.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.