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P08289

- PPBT_RAT

UniProt

P08289 - PPBT_RAT

Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (01 May 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601MagnesiumBy similarity
    Metal bindingi60 – 601Zinc 1By similarity
    Active sitei110 – 1101Phosphoserine intermediate
    Metal bindingi110 – 1101Zinc 1By similarity
    Metal bindingi173 – 1731MagnesiumBy similarity
    Metal bindingi332 – 3321MagnesiumBy similarity
    Metal bindingi337 – 3371Zinc 2By similarity
    Metal bindingi341 – 3411Zinc 2; via tele nitrogenBy similarity
    Metal bindingi378 – 3781Zinc 1By similarity
    Metal bindingi379 – 3791Zinc 1; via tele nitrogenBy similarity
    Metal bindingi454 – 4541Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: RGD
    2. metal ion binding Source: UniProtKB-KW
    3. pyrophosphatase activity Source: Ensembl

    GO - Biological processi

    1. cellular response to organic cyclic compound Source: Ensembl
    2. cementum mineralization Source: RGD
    3. dephosphorylation Source: RGD
    4. developmental process involved in reproduction Source: Ensembl
    5. endochondral ossification Source: Ensembl
    6. response to antibiotic Source: Ensembl
    7. response to glucocorticoid Source: RGD
    8. response to lipopolysaccharide Source: RGD
    9. response to vitamin D Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP08289.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1)
    Short name:
    AP-TNAP
    Short name:
    TNSALP
    Alternative name(s):
    Alkaline phosphatase liver/bone/kidney isozyme
    Gene namesi
    Name:Alpl
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi2100. Alpl.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular matrix Source: Ensembl
    3. extracellular membrane-bounded organelle Source: Ensembl
    4. extracellular space Source: RGD
    5. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17171 PublicationAdd
    BLAST
    Chaini18 – 501484Alkaline phosphatase, tissue-nonspecific isozymePRO_0000024027Add
    BLAST
    Propeptidei502 – 52423Removed in mature formSequence AnalysisPRO_0000024028Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi139 ↔ 201By similarity
    Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi489 ↔ 497By similarity
    Lipidationi501 – 5011GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP08289.
    PRIDEiP08289.

    PTM databases

    PhosphoSiteiP08289.
    UniCarbKBiP08289.

    Expressioni

    Gene expression databases

    GenevestigatoriP08289.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiP08289. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP08289.
    SMRiP08289. Positions 18-493.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    GeneTreeiENSGT00390000008704.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    InParanoidiP08289.
    KOiK01077.
    OMAiTWKSFKP.
    OrthoDBiEOG7SN8CH.
    PhylomeDBiP08289.
    TreeFamiTF323513.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08289-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILPFLVLAI GTCLTNSFVP EKEKDPSYWR QQAQETLKNA LKLQKLNTNV    50
    AKNIIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT 100
    YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI 150
    LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG 200
    CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL 250
    DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN 300
    RNNLTDPSLS EMVEVALRIL TKNPKGFFLL VEGGRIDHGH HEGKAKQALH 350
    EAVEMDEAIG KAGTMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP 400
    MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR 450
    HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA 500
    SSASSPSPGA LLLPLALFPL RTLF 524
    Length:524
    Mass (Da):57,659
    Last modified:May 1, 1992 - v2
    Checksum:iBD75A4B87117DF03
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121T → P in AAA41845. (PubMed:3422431)Curated
    Sequence conflicti116 – 1161A → Y(PubMed:2895632)Curated
    Sequence conflicti193 – 1931P → R in AAA41845. (PubMed:3422431)Curated
    Sequence conflicti234 – 2341V → E in CAA68703. (PubMed:2895632)Curated
    Sequence conflicti254 – 2541S → T in CAA68703. (PubMed:2895632)Curated
    Sequence conflicti331 – 3311V → E in CAA68703. (PubMed:2895632)Curated
    Sequence conflicti374 – 3741V → L in CAA68703. (PubMed:2895632)Curated
    Sequence conflicti380 – 3889SHVFTFGGY → HPTFSRLVA(PubMed:2895632)Curated
    Sequence conflicti391 – 3911R → Q(PubMed:2895632)Curated
    Sequence conflicti463 – 4631A → C in CAA68703. (PubMed:2895632)Curated
    Sequence conflicti474 – 4741V → I in CAA68703. (PubMed:2895632)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00714 mRNA. Translation: CAA68703.1.
    J03572 mRNA. Translation: AAA41845.1.
    X16028
    , X16029, X16030, X16031, X16032, X16033, X16034, X16035, X16036, X16037, X16038 Genomic DNA. Translation: CAA34160.1.
    BC088399 mRNA. Translation: AAH88399.1.
    PIRiA28114.
    S00289.
    RefSeqiNP_037191.1. NM_013059.1.
    XP_006239198.1. XM_006239136.1.
    XP_006239199.1. XM_006239137.1.
    UniGeneiRn.82764.

    Genome annotation databases

    EnsembliENSRNOT00000019004; ENSRNOP00000019004; ENSRNOG00000013954.
    GeneIDi25586.
    KEGGirno:25586.
    UCSCiRGD:2100. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00714 mRNA. Translation: CAA68703.1 .
    J03572 mRNA. Translation: AAA41845.1 .
    X16028
    , X16029 , X16030 , X16031 , X16032 , X16033 , X16034 , X16035 , X16036 , X16037 , X16038 Genomic DNA. Translation: CAA34160.1 .
    BC088399 mRNA. Translation: AAH88399.1 .
    PIRi A28114.
    S00289.
    RefSeqi NP_037191.1. NM_013059.1.
    XP_006239198.1. XM_006239136.1.
    XP_006239199.1. XM_006239137.1.
    UniGenei Rn.82764.

    3D structure databases

    ProteinModelPortali P08289.
    SMRi P08289. Positions 18-493.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08289. 1 interaction.

    Chemistry

    BindingDBi P08289.
    ChEMBLi CHEMBL2729.

    PTM databases

    PhosphoSitei P08289.
    UniCarbKBi P08289.

    Proteomic databases

    PaxDbi P08289.
    PRIDEi P08289.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000019004 ; ENSRNOP00000019004 ; ENSRNOG00000013954 .
    GeneIDi 25586.
    KEGGi rno:25586.
    UCSCi RGD:2100. rat.

    Organism-specific databases

    CTDi 249.
    RGDi 2100. Alpl.

    Phylogenomic databases

    eggNOGi COG1785.
    GeneTreei ENSGT00390000008704.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    InParanoidi P08289.
    KOi K01077.
    OMAi TWKSFKP.
    OrthoDBi EOG7SN8CH.
    PhylomeDBi P08289.
    TreeFami TF323513.

    Enzyme and pathway databases

    SABIO-RK P08289.

    Miscellaneous databases

    NextBioi 607251.
    PROi P08289.

    Gene expression databases

    Genevestigatori P08289.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of rat osteosarcoma (ROS 17/2.8) alkaline phosphatase: product of a single copy gene."
      Thiede M.A., Yoon K., Golub E.E., Noda M., Rodan G.A.
      Proc. Natl. Acad. Sci. U.S.A. 85:319-323(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Placenta.
    2. "Primary structure of rat liver alkaline phosphatase deduced from its cDNA."
      Misumi Y., Tashiro K., Hattori M., Sakaki Y., Ikehara Y.
      Biochem. J. 249:661-668(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Liver.
    3. "Isolation and characterization of a rat liver alkaline phosphatase gene. A single gene with two promoters."
      Toh Y., Yamamoto M., Endo H., Misumi Y., Ikehara Y.
      Eur. J. Biochem. 182:231-237(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    5. "Chemical identification of lipid components in the membranous form of rat liver alkaline phosphatase."
      Ogata S., Hayashi Y., Yasutake K., Ikehara Y.
      J. Biochem. 102:1609-1615(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-47, GPI-ANCHOR.
      Tissue: Liver.

    Entry informationi

    Entry nameiPPBT_RAT
    AccessioniPrimary (citable) accession number: P08289
    Secondary accession number(s): P14055, P70707
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3