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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601MagnesiumBy similarity
Metal bindingi60 – 601Zinc 1By similarity
Active sitei110 – 1101Phosphoserine intermediate
Metal bindingi110 – 1101Zinc 1By similarity
Metal bindingi173 – 1731MagnesiumBy similarity
Metal bindingi332 – 3321MagnesiumBy similarity
Metal bindingi337 – 3371Zinc 2By similarity
Metal bindingi341 – 3411Zinc 2; via tele nitrogenBy similarity
Metal bindingi378 – 3781Zinc 1By similarity
Metal bindingi379 – 3791Zinc 1; via tele nitrogenBy similarity
Metal bindingi454 – 4541Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  • alkaline phosphatase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • pyrophosphatase activity Source: Ensembl

GO - Biological processi

  • cellular response to organic cyclic compound Source: Ensembl
  • cementum mineralization Source: RGD
  • dephosphorylation Source: RGD
  • developmental process involved in reproduction Source: Ensembl
  • endochondral ossification Source: Ensembl
  • osteoblast differentiation Source: Ensembl
  • response to antibiotic Source: Ensembl
  • response to glucocorticoid Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to vitamin D Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1. 5301.
SABIO-RKP08289.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:Alpl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2100. Alpl.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular exosome Source: Ensembl
  • extracellular matrix Source: Ensembl
  • extracellular space Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Chaini18 – 501484Alkaline phosphatase, tissue-nonspecific isozymePRO_0000024027Add
BLAST
Propeptidei502 – 52423Removed in mature formSequence AnalysisPRO_0000024028Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi489 ↔ 497By similarity
Lipidationi501 – 5011GPI-anchor amidated serineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08289.
PRIDEiP08289.

PTM databases

PhosphoSiteiP08289.
UniCarbKBiP08289.

Expressioni

Gene expression databases

GenevisibleiP08289. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP08289. 1 interaction.
STRINGi10116.ENSRNOP00000019004.

Structurei

3D structure databases

ProteinModelPortaliP08289.
SMRiP08289. Positions 18-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP08289.
KOiK01077.
OMAiMQEALVP.
OrthoDBiEOG7SN8CH.
PhylomeDBiP08289.
TreeFamiTF323513.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILPFLVLAI GTCLTNSFVP EKEKDPSYWR QQAQETLKNA LKLQKLNTNV
60 70 80 90 100
AKNIIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNLTDPSLS EMVEVALRIL TKNPKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDEAIG KAGTMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA
510 520
SSASSPSPGA LLLPLALFPL RTLF
Length:524
Mass (Da):57,659
Last modified:May 1, 1992 - v2
Checksum:iBD75A4B87117DF03
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121T → P in AAA41845 (PubMed:3422431).Curated
Sequence conflicti116 – 1161A → Y (PubMed:2895632).Curated
Sequence conflicti193 – 1931P → R in AAA41845 (PubMed:3422431).Curated
Sequence conflicti234 – 2341V → E in CAA68703 (PubMed:2895632).Curated
Sequence conflicti254 – 2541S → T in CAA68703 (PubMed:2895632).Curated
Sequence conflicti331 – 3311V → E in CAA68703 (PubMed:2895632).Curated
Sequence conflicti374 – 3741V → L in CAA68703 (PubMed:2895632).Curated
Sequence conflicti380 – 3889SHVFTFGGY → HPTFSRLVA (PubMed:2895632).Curated
Sequence conflicti391 – 3911R → Q (PubMed:2895632).Curated
Sequence conflicti463 – 4631A → C in CAA68703 (PubMed:2895632).Curated
Sequence conflicti474 – 4741V → I in CAA68703 (PubMed:2895632).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00714 mRNA. Translation: CAA68703.1.
J03572 mRNA. Translation: AAA41845.1.
X16028
, X16029, X16030, X16031, X16032, X16033, X16034, X16035, X16036, X16037, X16038 Genomic DNA. Translation: CAA34160.1.
BC088399 mRNA. Translation: AAH88399.1.
PIRiA28114.
S00289.
RefSeqiNP_037191.1. NM_013059.1.
XP_006239198.1. XM_006239136.2.
UniGeneiRn.82764.

Genome annotation databases

EnsembliENSRNOT00000019004; ENSRNOP00000019004; ENSRNOG00000013954.
GeneIDi25586.
KEGGirno:25586.
UCSCiRGD:2100. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00714 mRNA. Translation: CAA68703.1.
J03572 mRNA. Translation: AAA41845.1.
X16028
, X16029, X16030, X16031, X16032, X16033, X16034, X16035, X16036, X16037, X16038 Genomic DNA. Translation: CAA34160.1.
BC088399 mRNA. Translation: AAH88399.1.
PIRiA28114.
S00289.
RefSeqiNP_037191.1. NM_013059.1.
XP_006239198.1. XM_006239136.2.
UniGeneiRn.82764.

3D structure databases

ProteinModelPortaliP08289.
SMRiP08289. Positions 18-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08289. 1 interaction.
STRINGi10116.ENSRNOP00000019004.

Chemistry

BindingDBiP08289.
ChEMBLiCHEMBL2729.

PTM databases

PhosphoSiteiP08289.
UniCarbKBiP08289.

Proteomic databases

PaxDbiP08289.
PRIDEiP08289.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019004; ENSRNOP00000019004; ENSRNOG00000013954.
GeneIDi25586.
KEGGirno:25586.
UCSCiRGD:2100. rat.

Organism-specific databases

CTDi249.
RGDi2100. Alpl.

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP08289.
KOiK01077.
OMAiMQEALVP.
OrthoDBiEOG7SN8CH.
PhylomeDBiP08289.
TreeFamiTF323513.

Enzyme and pathway databases

BRENDAi3.1.3.1. 5301.
SABIO-RKP08289.

Miscellaneous databases

NextBioi607251.
PROiP08289.

Gene expression databases

GenevisibleiP08289. RN.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of rat osteosarcoma (ROS 17/2.8) alkaline phosphatase: product of a single copy gene."
    Thiede M.A., Yoon K., Golub E.E., Noda M., Rodan G.A.
    Proc. Natl. Acad. Sci. U.S.A. 85:319-323(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "Primary structure of rat liver alkaline phosphatase deduced from its cDNA."
    Misumi Y., Tashiro K., Hattori M., Sakaki Y., Ikehara Y.
    Biochem. J. 249:661-668(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  3. "Isolation and characterization of a rat liver alkaline phosphatase gene. A single gene with two promoters."
    Toh Y., Yamamoto M., Endo H., Misumi Y., Ikehara Y.
    Eur. J. Biochem. 182:231-237(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  5. "Chemical identification of lipid components in the membranous form of rat liver alkaline phosphatase."
    Ogata S., Hayashi Y., Yasutake K., Ikehara Y.
    J. Biochem. 102:1609-1615(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-47, GPI-ANCHOR.
    Tissue: Liver.

Entry informationi

Entry nameiPPBT_RAT
AccessioniPrimary (citable) accession number: P08289
Secondary accession number(s): P14055, P70707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 1992
Last modified: June 24, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.