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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This isozyme may play a role in skeletal mineralization.By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60MagnesiumBy similarity1
Metal bindingi60Zinc 1By similarity1
Active sitei110Phosphoserine intermediateBy similarity1
Metal bindingi110Zinc 1By similarity1
Metal bindingi173MagnesiumBy similarity1
Metal bindingi332MagnesiumBy similarity1
Metal bindingi337Zinc 2By similarity1
Metal bindingi341Zinc 2; via tele nitrogenBy similarity1
Metal bindingi378Zinc 1By similarity1
Metal bindingi379Zinc 1; via tele nitrogenBy similarity1
Metal bindingi454Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • pyrophosphatase activity Source: Ensembl

GO - Biological processi

  • cellular response to organic cyclic compound Source: Ensembl
  • cementum mineralization Source: RGD
  • dephosphorylation Source: RGD
  • developmental process involved in reproduction Source: Ensembl
  • endochondral ossification Source: Ensembl
  • osteoblast differentiation Source: Ensembl
  • response to antibiotic Source: Ensembl
  • response to glucocorticoid Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to vitamin D Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1. 5301.
SABIO-RKP08289.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.11 Publication)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:Alpl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2100. Alpl.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2729.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000002402718 – 501Alkaline phosphatase, tissue-nonspecific isozymeAdd BLAST484
PropeptideiPRO_0000024028502 – 524Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140N-linked (GlcNAc...)Sequence analysis1
Glycosylationi230N-linked (GlcNAc...)Sequence analysis1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi303N-linked (GlcNAc...)Sequence analysis1
Glycosylationi430N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi489 ↔ 497By similarity
Lipidationi501GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08289.
PRIDEiP08289.

PTM databases

iPTMnetiP08289.
PhosphoSitePlusiP08289.
UniCarbKBiP08289.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013954.
ExpressionAtlasiP08289. baseline and differential.
GenevisibleiP08289. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP08289. 1 interactor.
STRINGi10116.ENSRNOP00000019004.

Chemistry databases

BindingDBiP08289.

Structurei

3D structure databases

ProteinModelPortaliP08289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP08289.
KOiK01077.
OMAiYTYVTTG.
OrthoDBiEOG091G067H.
PhylomeDBiP08289.
TreeFamiTF323513.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILPFLVLAI GTCLTNSFVP EKEKDPSYWR QQAQETLKNA LKLQKLNTNV
60 70 80 90 100
AKNIIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNLTDPSLS EMVEVALRIL TKNPKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDEAIG KAGTMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA
510 520
SSASSPSPGA LLLPLALFPL RTLF
Length:524
Mass (Da):57,659
Last modified:May 1, 1992 - v2
Checksum:iBD75A4B87117DF03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12T → P in AAA41845 (PubMed:3422431).Curated1
Sequence conflicti116A → Y (PubMed:2895632).Curated1
Sequence conflicti193P → R in AAA41845 (PubMed:3422431).Curated1
Sequence conflicti234V → E in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti254S → T in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti331V → E in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti374V → L in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti380 – 388SHVFTFGGY → HPTFSRLVA (PubMed:2895632).Curated9
Sequence conflicti391R → Q (PubMed:2895632).Curated1
Sequence conflicti463A → C in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti474V → I in CAA68703 (PubMed:2895632).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00714 mRNA. Translation: CAA68703.1.
J03572 mRNA. Translation: AAA41845.1.
X16028
, X16029, X16030, X16031, X16032, X16033, X16034, X16035, X16036, X16037, X16038 Genomic DNA. Translation: CAA34160.1.
BC088399 mRNA. Translation: AAH88399.1.
PIRiA28114.
S00289.
RefSeqiNP_037191.1. NM_013059.1.
XP_006239198.1. XM_006239136.3.
XP_017448654.1. XM_017593165.1.
UniGeneiRn.82764.

Genome annotation databases

EnsembliENSRNOT00000019004; ENSRNOP00000019004; ENSRNOG00000013954.
GeneIDi25586.
KEGGirno:25586.
UCSCiRGD:2100. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00714 mRNA. Translation: CAA68703.1.
J03572 mRNA. Translation: AAA41845.1.
X16028
, X16029, X16030, X16031, X16032, X16033, X16034, X16035, X16036, X16037, X16038 Genomic DNA. Translation: CAA34160.1.
BC088399 mRNA. Translation: AAH88399.1.
PIRiA28114.
S00289.
RefSeqiNP_037191.1. NM_013059.1.
XP_006239198.1. XM_006239136.3.
XP_017448654.1. XM_017593165.1.
UniGeneiRn.82764.

3D structure databases

ProteinModelPortaliP08289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08289. 1 interactor.
STRINGi10116.ENSRNOP00000019004.

Chemistry databases

BindingDBiP08289.
ChEMBLiCHEMBL2729.

PTM databases

iPTMnetiP08289.
PhosphoSitePlusiP08289.
UniCarbKBiP08289.

Proteomic databases

PaxDbiP08289.
PRIDEiP08289.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019004; ENSRNOP00000019004; ENSRNOG00000013954.
GeneIDi25586.
KEGGirno:25586.
UCSCiRGD:2100. rat.

Organism-specific databases

CTDi249.
RGDi2100. Alpl.

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP08289.
KOiK01077.
OMAiYTYVTTG.
OrthoDBiEOG091G067H.
PhylomeDBiP08289.
TreeFamiTF323513.

Enzyme and pathway databases

BRENDAi3.1.3.1. 5301.
SABIO-RKP08289.

Miscellaneous databases

PROiP08289.

Gene expression databases

BgeeiENSRNOG00000013954.
ExpressionAtlasiP08289. baseline and differential.
GenevisibleiP08289. RN.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPBT_RAT
AccessioniPrimary (citable) accession number: P08289
Secondary accession number(s): P14055, P70707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 1992
Last modified: November 30, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.