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P08289 (PPBT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase, tissue-nonspecific isozyme

Short name=AP-TNAP
Short name=TNSALP
EC=3.1.3.1
Alternative name(s):
Alkaline phosphatase liver/bone/kidney isozyme
Gene names
Name:Alpl
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Ref.5.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to organic cyclic compound

Inferred from electronic annotation. Source: Ensembl

cementum mineralization

Inferred from expression pattern PubMed 17043865. Source: RGD

dephosphorylation

Inferred from mutant phenotype PubMed 18288101. Source: RGD

developmental process involved in reproduction

Inferred from electronic annotation. Source: Ensembl

endochondral ossification

Inferred from electronic annotation. Source: Ensembl

response to antibiotic

Inferred from electronic annotation. Source: Ensembl

response to glucocorticoid

Inferred from direct assay PubMed 2039500. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern PubMed 11810315. Source: RGD

response to vitamin D

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentanchored component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

extracellular matrix

Inferred from electronic annotation. Source: Ensembl

extracellular membrane-bounded organelle

Inferred from electronic annotation. Source: Ensembl

extracellular space

Inferred from direct assay PubMed 19697513. Source: RGD

plasma membrane

Inferred from direct assay PubMed 19697513. Source: RGD

   Molecular_functionalkaline phosphatase activity

Inferred from direct assay PubMed 3472490. Source: RGD

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

pyrophosphatase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.5
Chain18 – 501484Alkaline phosphatase, tissue-nonspecific isozyme
PRO_0000024027
Propeptide502 – 52423Removed in mature form Potential
PRO_0000024028

Sites

Active site1101Phosphoserine intermediate
Metal binding601Magnesium By similarity
Metal binding601Zinc 1 By similarity
Metal binding1101Zinc 1 By similarity
Metal binding1731Magnesium By similarity
Metal binding3321Magnesium By similarity
Metal binding3371Zinc 2 By similarity
Metal binding3411Zinc 2; via tele nitrogen By similarity
Metal binding3781Zinc 1 By similarity
Metal binding3791Zinc 1; via tele nitrogen By similarity
Metal binding4541Zinc 2; via tele nitrogen By similarity

Amino acid modifications

Lipidation5011GPI-anchor amidated serine Potential
Glycosylation1401N-linked (GlcNAc...) Potential
Glycosylation2301N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation4301N-linked (GlcNAc...) Potential
Disulfide bond139 ↔ 201 By similarity
Disulfide bond489 ↔ 497 By similarity

Experimental info

Sequence conflict121T → P in AAA41845. Ref.1
Sequence conflict1161A → Y Ref.2
Sequence conflict1931P → R in AAA41845. Ref.1
Sequence conflict2341V → E in CAA68703. Ref.2
Sequence conflict2541S → T in CAA68703. Ref.2
Sequence conflict3311V → E in CAA68703. Ref.2
Sequence conflict3741V → L in CAA68703. Ref.2
Sequence conflict380 – 3889SHVFTFGGY → HPTFSRLVA Ref.2
Sequence conflict3911R → Q Ref.2
Sequence conflict4631A → C in CAA68703. Ref.2
Sequence conflict4741V → I in CAA68703. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P08289 [UniParc].

Last modified May 1, 1992. Version 2.
Checksum: BD75A4B87117DF03

FASTA52457,659
        10         20         30         40         50         60 
MILPFLVLAI GTCLTNSFVP EKEKDPSYWR QQAQETLKNA LKLQKLNTNV AKNIIMFLGD 

        70         80         90        100        110        120 
GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT YNTNAQVPDS AGTATAYLCG 

       130        140        150        160        170        180 
VKANEGTVGV SAATERTRCN TTQGNEVTSI LRWAKDAGKS VGIVTTTRVN HATPSAAYAH 

       190        200        210        220        230        240 
SADRDWYSDN EMPPEALSQG CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE 

       250        260        270        280        290        300 
KARGTRLDGL DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN 

       310        320        330        340        350        360 
RNNLTDPSLS EMVEVALRIL TKNPKGFFLL VEGGRIDHGH HEGKAKQALH EAVEMDEAIG 

       370        380        390        400        410        420 
KAGTMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP MVSDTDKKPF TAILYGNGPG 

       430        440        450        460        470        480 
YKVVDGEREN VSMVDYAHNN YQAQSAVPLR HETHGGEDVA VFAKGPMAHL LHGVHEQNYI 

       490        500        510        520 
PHVMAYASCI GANLDHCAWA SSASSPSPGA LLLPLALFPL RTLF 

« Hide

References

« Hide 'large scale' references
[1]"Structure and expression of rat osteosarcoma (ROS 17/2.8) alkaline phosphatase: product of a single copy gene."
Thiede M.A., Yoon K., Golub E.E., Noda M., Rodan G.A.
Proc. Natl. Acad. Sci. U.S.A. 85:319-323(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"Primary structure of rat liver alkaline phosphatase deduced from its cDNA."
Misumi Y., Tashiro K., Hattori M., Sakaki Y., Ikehara Y.
Biochem. J. 249:661-668(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Liver.
[3]"Isolation and characterization of a rat liver alkaline phosphatase gene. A single gene with two promoters."
Toh Y., Yamamoto M., Endo H., Misumi Y., Ikehara Y.
Eur. J. Biochem. 182:231-237(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[5]"Chemical identification of lipid components in the membranous form of rat liver alkaline phosphatase."
Ogata S., Hayashi Y., Yasutake K., Ikehara Y.
J. Biochem. 102:1609-1615(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 18-47, GPI-ANCHOR.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00714 mRNA. Translation: CAA68703.1.
J03572 mRNA. Translation: AAA41845.1.
X16028 expand/collapse EMBL AC list , X16029, X16030, X16031, X16032, X16033, X16034, X16035, X16036, X16037, X16038 Genomic DNA. Translation: CAA34160.1.
BC088399 mRNA. Translation: AAH88399.1.
PIRA28114.
S00289.
RefSeqNP_037191.1. NM_013059.1.
XP_006239198.1. XM_006239136.1.
XP_006239199.1. XM_006239137.1.
UniGeneRn.82764.

3D structure databases

ProteinModelPortalP08289.
SMRP08289. Positions 18-493.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP08289. 1 interaction.

Chemistry

BindingDBP08289.
ChEMBLCHEMBL2729.

PTM databases

PhosphoSiteP08289.
UniCarbKBP08289.

Proteomic databases

PaxDbP08289.
PRIDEP08289.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000019004; ENSRNOP00000019004; ENSRNOG00000013954.
GeneID25586.
KEGGrno:25586.
UCSCRGD:2100. rat.

Organism-specific databases

CTD249.
RGD2100. Alpl.

Phylogenomic databases

eggNOGCOG1785.
GeneTreeENSGT00390000008704.
HOGENOMHOG000099118.
HOVERGENHBG007345.
InParanoidP08289.
KOK01077.
OMAMQEALVP.
OrthoDBEOG7SN8CH.
PhylomeDBP08289.
TreeFamTF323513.

Enzyme and pathway databases

SABIO-RKP08289.

Gene expression databases

GenevestigatorP08289.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio607251.
PROP08289.

Entry information

Entry namePPBT_RAT
AccessionPrimary (citable) accession number: P08289
Secondary accession number(s): P14055, P70707
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families