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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This isozyme may play a role in skeletal mineralization.By similarity

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60MagnesiumBy similarity1
Metal bindingi60Zinc 1By similarity1
Active sitei110Phosphoserine intermediateBy similarity1
Metal bindingi110Zinc 1By similarity1
Metal bindingi173MagnesiumBy similarity1
Metal bindingi332MagnesiumBy similarity1
Metal bindingi337Zinc 2By similarity1
Metal bindingi341Zinc 2; via tele nitrogenBy similarity1
Metal bindingi378Zinc 1By similarity1
Metal bindingi379Zinc 1; via tele nitrogenBy similarity1
Metal bindingi454Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • pyrophosphatase activity Source: Ensembl

GO - Biological processi

  • cellular response to organic cyclic compound Source: Ensembl
  • cementum mineralization Source: RGD
  • dephosphorylation Source: RGD
  • developmental process involved in reproduction Source: Ensembl
  • endochondral ossification Source: Ensembl
  • response to antibiotic Source: Ensembl
  • response to glucocorticoid Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to vitamin D Source: Ensembl

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1 5301
ReactomeiR-RNO-163125 Post-translational modification: synthesis of GPI-anchored proteins
SABIO-RKiP08289

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.11 Publication)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:Alpl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2100 Alpl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2729

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000002402718 – 501Alkaline phosphatase, tissue-nonspecific isozymeAdd BLAST484
PropeptideiPRO_0000024028502 – 524Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi230N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi430N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi489 ↔ 497By similarity
Lipidationi501GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08289
PRIDEiP08289

PTM databases

GlyConnecti12
iPTMnetiP08289
PhosphoSitePlusiP08289
UniCarbKBiP08289

Expressioni

Gene expression databases

BgeeiENSRNOG00000013954
ExpressionAtlasiP08289 baseline and differential
GenevisibleiP08289 RN

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP08289, 1 interactor
STRINGi10116.ENSRNOP00000019004

Chemistry databases

BindingDBiP08289

Structurei

3D structure databases

ProteinModelPortaliP08289
SMRiP08289
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126 Eukaryota
COG1785 LUCA
GeneTreeiENSGT00390000008704
HOGENOMiHOG000099118
HOVERGENiHBG007345
InParanoidiP08289
KOiK01077
OMAiEHTGTQL
OrthoDBiEOG091G067H
PhylomeDBiP08289
TreeFamiTF323513

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILPFLVLAI GTCLTNSFVP EKEKDPSYWR QQAQETLKNA LKLQKLNTNV
60 70 80 90 100
AKNIIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNLTDPSLS EMVEVALRIL TKNPKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDEAIG KAGTMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA
510 520
SSASSPSPGA LLLPLALFPL RTLF
Length:524
Mass (Da):57,659
Last modified:May 1, 1992 - v2
Checksum:iBD75A4B87117DF03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12T → P in AAA41845 (PubMed:3422431).Curated1
Sequence conflicti116A → Y (PubMed:2895632).Curated1
Sequence conflicti193P → R in AAA41845 (PubMed:3422431).Curated1
Sequence conflicti234V → E in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti254S → T in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti331V → E in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti374V → L in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti380 – 388SHVFTFGGY → HPTFSRLVA (PubMed:2895632).Curated9
Sequence conflicti391R → Q (PubMed:2895632).Curated1
Sequence conflicti463A → C in CAA68703 (PubMed:2895632).Curated1
Sequence conflicti474V → I in CAA68703 (PubMed:2895632).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00714 mRNA Translation: CAA68703.1
J03572 mRNA Translation: AAA41845.1
X16028
, X16029, X16030, X16031, X16032, X16033, X16034, X16035, X16036, X16037, X16038 Genomic DNA Translation: CAA34160.1
BC088399 mRNA Translation: AAH88399.1
PIRiA28114
S00289
RefSeqiNP_037191.1, NM_013059.1
XP_006239198.1, XM_006239136.3
XP_017448654.1, XM_017593165.1
UniGeneiRn.82764

Genome annotation databases

EnsembliENSRNOT00000019004; ENSRNOP00000019004; ENSRNOG00000013954
GeneIDi25586
KEGGirno:25586
UCSCiRGD:2100 rat

Similar proteinsi

Entry informationi

Entry nameiPPBT_RAT
AccessioniPrimary (citable) accession number: P08289
Secondary accession number(s): P14055, P70707
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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