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Protein

Glutamine synthetase nodule isozyme

Gene

GS1

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Miscellaneous

In pea there are distinct isozymes in leaves, roots and nodules.
Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).
This root isozyme is responsible for the assimilation of ammonia fixed by bacteroids.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase nodule isozyme (EC:6.3.1.2)
Alternative name(s):
Cytosolic GS1
Glutamate--ammonia ligase
Gene namesi
Name:GS1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001531881 – 355Glutamine synthetase nodule isozymeAdd BLAST355

Proteomic databases

PRIDEiP08282.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP08282.
SMRiP08282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P08282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSDLINLD LSGTTEKIIA EYIWIGGSGL DLRCKARTLP GPVTDPSELP
60 70 80 90 100
KWNYDGSSTG QAPGQDSEVI LYPQAIFKDP FRRGNHILVM CDAYSPAGEP
110 120 130 140 150
IPTNKRHAAA KVFSHPDVVA EETWYGIEQE YTLLQKDINW PLGWPAGGYP
160 170 180 190 200
GPQGPYYCSV GADKAFGRDV VEAHYKACLF AGINISGING EVMPGQWEFQ
210 220 230 240 250
VGPSVGISAG DEIWVARYIL ERITEVAGVV LTFDPKPIKG DWNGAGAHTN
260 270 280 290 300
YSTKSMREDG GYEIIKKAIE KLGKRLPEHI SAYGEGNERR LTGKHETADI
310 320 330 340 350
NTFSWGVANR GASVRVGRDT EKEGKGYFED RRPASNMDPY VVTSMIAETT

ILLKP
Length:355
Mass (Da):38,928
Last modified:July 1, 1989 - v2
Checksum:i2AB833FAD7AA3687
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20663 mRNA. Translation: AAA33669.1.
X05515 mRNA. Translation: CAA29058.1.
PIRiB28089. AJPMQ1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLNA1_PEA
AccessioniPrimary (citable) accession number: P08282
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 1, 1989
Last modified: March 15, 2017
This is version 84 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families