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P08282

- GLNA1_PEA

UniProt

P08282 - GLNA1_PEA

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Protein

Glutamine synthetase nodule isozyme

Gene

GS1

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase nodule isozyme (EC:6.3.1.2)
Alternative name(s):
Cytosolic GS1
Glutamate--ammonia ligase
Gene namesi
Name:GS1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Glutamine synthetase nodule isozymePRO_0000153188Add
BLAST

Proteomic databases

PRIDEiP08282.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP08282.
SMRiP08282. Positions 3-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08282-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLSDLINLD LSGTTEKIIA EYIWIGGSGL DLRCKARTLP GPVTDPSELP
60 70 80 90 100
KWNYDGSSTG QAPGQDSEVI LYPQAIFKDP FRRGNHILVM CDAYSPAGEP
110 120 130 140 150
IPTNKRHAAA KVFSHPDVVA EETWYGIEQE YTLLQKDINW PLGWPAGGYP
160 170 180 190 200
GPQGPYYCSV GADKAFGRDV VEAHYKACLF AGINISGING EVMPGQWEFQ
210 220 230 240 250
VGPSVGISAG DEIWVARYIL ERITEVAGVV LTFDPKPIKG DWNGAGAHTN
260 270 280 290 300
YSTKSMREDG GYEIIKKAIE KLGKRLPEHI SAYGEGNERR LTGKHETADI
310 320 330 340 350
NTFSWGVANR GASVRVGRDT EKEGKGYFED RRPASNMDPY VVTSMIAETT

ILLKP
Length:355
Mass (Da):38,928
Last modified:July 1, 1989 - v2
Checksum:i2AB833FAD7AA3687
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20663 mRNA. Translation: AAA33669.1.
X05515 mRNA. Translation: CAA29058.1.
PIRiB28089. AJPMQ1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20663 mRNA. Translation: AAA33669.1 .
X05515 mRNA. Translation: CAA29058.1 .
PIRi B28089. AJPMQ1.

3D structure databases

ProteinModelPortali P08282.
SMRi P08282. Positions 3-354.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P08282.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chloroplast and cytosolic glutamine synthetase are encoded by homologous nuclear genes which are differentially expressed in vivo."
    Tingey S.V., Tsai F., Edwards J., Walker E.L., Coruzzi G.M.
    J. Biol. Chem. 263:9651-9657(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules."
    Tingey S.V., Walker E.L., Coruzzi G.M.
    EMBO J. 6:1-9(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 97-355.
    Strain: cv. Sparkle.

Entry informationi

Entry nameiGLNA1_PEA
AccessioniPrimary (citable) accession number: P08282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In pea there are distinct isozymes in leaves, roots and nodules.
Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).
This root isozyme is responsible for the assimilation of ammonia fixed by bacteroids.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3