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P08281 (GLNA2_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase leaf isozyme, chloroplastic

EC=6.3.1.2
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene names
Name:GS2
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Plastidchloroplast.

Miscellaneous

In pea there are distinct isozymes in leaves, roots and nodules.

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast
Chain50 – 430381Glutamine synthetase leaf isozyme, chloroplastic
PRO_0000011182

Sequences

Sequence LengthMass (Da)Tools
P08281 [UniParc].

Last modified July 1, 1989. Version 2.
Checksum: AB7EFA4F53970D7E

FASTA43047,346
        10         20         30         40         50         60 
MAQILAPSTQ WQMRITKTSP CATPITSKMW SSLVMKQTKK VAHSAKFRVM AVNSENGTIN 

        70         80         90        100        110        120 
RVEDLLNLDI TPFTDSIIAE YIWIGGTGID VRSKSRTISK PVSHPSEVPK WNYDGSSTGQ 

       130        140        150        160        170        180 
APGEDSEVIL YPQAIFKDPF RGGNNILVVC DAYTPAGEPI PTNKRHRAAE IFSNPKVEAE 

       190        200        210        220        230        240 
IPWYGIEQEY TLLQTNVKWP LGWPVGGYPG PQGPYYCAAG ADKSFGRDIS DAHYKACIYA 

       250        260        270        280        290        300 
GINISGTNGE VMPGQWEYQV GPSVGIEAGD HIWASRYILE RITEQAGVVL TLDPKPIEGD 

       310        320        330        340        350        360 
WNGAGCHTNY STKSMREDGG FEVIKKAILN LSLRHKIHIE AYGEGNERRL TGKHETASIN 

       370        380        390        400        410        420 
DFSWGVANRG CSIRVGRDTE KNGKGYLEDR RPASNMDPYV VTALLAESTL LWEPTLEAEA 

       430 
LAAQKIALKV 

« Hide

References

[1]"Chloroplast and cytosolic glutamine synthetase are encoded by homologous nuclear genes which are differentially expressed in vivo."
Tingey S.V., Tsai F., Edwards J., Walker E.L., Coruzzi G.M.
J. Biol. Chem. 263:9651-9657(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules."
Tingey S.V., Walker E.L., Coruzzi G.M.
EMBO J. 6:1-9(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-430.
Strain: cv. Sparkle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20664 mRNA. Translation: AAA33653.1.
X05514 mRNA. Translation: CAA29057.1.
PIRAJPMQ2. A28089.

3D structure databases

ProteinModelPortalP08281.
SMRP08281. Positions 64-413.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA2_PEA
AccessionPrimary (citable) accession number: P08281
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 1, 1989
Last modified: February 19, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families