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P08281

- GLNA2_PEA

UniProt

P08281 - GLNA2_PEA

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Protein
Glutamine synthetase leaf isozyme, chloroplastic
Gene
GS2
Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase leaf isozyme, chloroplastic (EC:6.3.1.2)
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene namesi
Name:GS2
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4949Chloroplast
Add
BLAST
Chaini50 – 430381Glutamine synthetase leaf isozyme, chloroplastic
PRO_0000011182Add
BLAST

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP08281.
SMRiP08281. Positions 64-413.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08281-1 [UniParc]FASTAAdd to Basket

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MAQILAPSTQ WQMRITKTSP CATPITSKMW SSLVMKQTKK VAHSAKFRVM    50
AVNSENGTIN RVEDLLNLDI TPFTDSIIAE YIWIGGTGID VRSKSRTISK 100
PVSHPSEVPK WNYDGSSTGQ APGEDSEVIL YPQAIFKDPF RGGNNILVVC 150
DAYTPAGEPI PTNKRHRAAE IFSNPKVEAE IPWYGIEQEY TLLQTNVKWP 200
LGWPVGGYPG PQGPYYCAAG ADKSFGRDIS DAHYKACIYA GINISGTNGE 250
VMPGQWEYQV GPSVGIEAGD HIWASRYILE RITEQAGVVL TLDPKPIEGD 300
WNGAGCHTNY STKSMREDGG FEVIKKAILN LSLRHKIHIE AYGEGNERRL 350
TGKHETASIN DFSWGVANRG CSIRVGRDTE KNGKGYLEDR RPASNMDPYV 400
VTALLAESTL LWEPTLEAEA LAAQKIALKV 430
Length:430
Mass (Da):47,346
Last modified:July 1, 1989 - v2
Checksum:iAB7EFA4F53970D7E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20664 mRNA. Translation: AAA33653.1.
X05514 mRNA. Translation: CAA29057.1.
PIRiA28089. AJPMQ2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20664 mRNA. Translation: AAA33653.1 .
X05514 mRNA. Translation: CAA29057.1 .
PIRi A28089. AJPMQ2.

3D structure databases

ProteinModelPortali P08281.
SMRi P08281. Positions 64-413.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chloroplast and cytosolic glutamine synthetase are encoded by homologous nuclear genes which are differentially expressed in vivo."
    Tingey S.V., Tsai F., Edwards J., Walker E.L., Coruzzi G.M.
    J. Biol. Chem. 263:9651-9657(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules."
    Tingey S.V., Walker E.L., Coruzzi G.M.
    EMBO J. 6:1-9(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 58-430.
    Strain: cv. Sparkle.

Entry informationi

Entry nameiGLNA2_PEA
AccessioniPrimary (citable) accession number: P08281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 1, 1989
Last modified: February 19, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In pea there are distinct isozymes in leaves, roots and nodules.
Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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