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Protein

Glutamine synthetase leaf isozyme, chloroplastic

Gene

GS2

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.

Miscellaneous

In pea there are distinct isozymes in leaves, roots and nodules.
Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase leaf isozyme, chloroplastic (EC:6.3.1.2)
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene namesi
Name:GS2
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 49ChloroplastAdd BLAST49
ChainiPRO_000001118250 – 430Glutamine synthetase leaf isozyme, chloroplasticAdd BLAST381

Proteomic databases

PRIDEiP08281

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP08281
SMRiP08281
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.10.20.70, 1 hit
InterProiView protein in InterPro
IPR008147 Gln_synt_b-grasp
IPR036651 Gln_synt_N
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR008146 Gln_synth_cat_dom
IPR027303 Gln_synth_gly_rich_site
IPR027302 Gln_synth_N_conserv_site
PfamiView protein in Pfam
PF00120 Gln-synt_C, 1 hit
PF03951 Gln-synt_N, 1 hit
SMARTiView protein in SMART
SM01230 Gln-synt_C, 1 hit
SUPFAMiSSF54368 SSF54368, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00180 GLNA_1, 1 hit
PS00181 GLNA_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08281-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQILAPSTQ WQMRITKTSP CATPITSKMW SSLVMKQTKK VAHSAKFRVM
60 70 80 90 100
AVNSENGTIN RVEDLLNLDI TPFTDSIIAE YIWIGGTGID VRSKSRTISK
110 120 130 140 150
PVSHPSEVPK WNYDGSSTGQ APGEDSEVIL YPQAIFKDPF RGGNNILVVC
160 170 180 190 200
DAYTPAGEPI PTNKRHRAAE IFSNPKVEAE IPWYGIEQEY TLLQTNVKWP
210 220 230 240 250
LGWPVGGYPG PQGPYYCAAG ADKSFGRDIS DAHYKACIYA GINISGTNGE
260 270 280 290 300
VMPGQWEYQV GPSVGIEAGD HIWASRYILE RITEQAGVVL TLDPKPIEGD
310 320 330 340 350
WNGAGCHTNY STKSMREDGG FEVIKKAILN LSLRHKIHIE AYGEGNERRL
360 370 380 390 400
TGKHETASIN DFSWGVANRG CSIRVGRDTE KNGKGYLEDR RPASNMDPYV
410 420 430
VTALLAESTL LWEPTLEAEA LAAQKIALKV
Length:430
Mass (Da):47,346
Last modified:July 1, 1989 - v2
Checksum:iAB7EFA4F53970D7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20664 mRNA Translation: AAA33653.1
X05514 mRNA Translation: CAA29057.1
PIRiA28089 AJPMQ2

Similar proteinsi

Entry informationi

Entry nameiGLNA2_PEA
AccessioniPrimary (citable) accession number: P08281
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health