Reviewed, UniProtKB/Swiss-Prot P08262 (HEM1_BRAJA)

Names and origin

Protein names

Recommended name:
    5-aminolevulinate synthase
    EC=2.3.1.37
Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-aminolevulinate synthase
    Delta-ALA synthetase

Gene names

Name:	hemA
Ordered Locus Names:	bll1200

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	5-aminolevulinate synthase		PRO_0000163825
Amino acid modifications
	Modified residue	247	1	N6-(pyridoxal phosphate)lysine Probable
Experimental info
	Sequence conflict	261	1	Q → R in AAA26216. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P08262-1 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: B21B011EB9542D66	FASTA	409	44,574
	10 20 30 40 50 60 MDYSQFFNSA LDRLHTERRY RVFADLERMA GRFPHAIWHS PKGKRDVVIW CSNDYLGMGQ 70 80 90 100 110 120 HPKVVGAMVE TATRVGTGAG GTRNIAGTHH PLVQLEAELA DLHGKEAALL FTSGYVSNQT 130 140 150 160 170 180 GIATIAKLIP NCLILSDELN HNSMIEGIRQ SGCERQVFRH NDLADLEALL KAAGANRPKL 190 200 210 220 230 240 IACESLYSMD GDVAPLAKIC DLAEKYNAMT YVDEVHAVGM YGPRGGGIAE RDGVMHRIDI 250 260 270 280 290 300 LEGTLAKAFG CLGGYIAANG QIIDAVRSYA PGFIFTTALP PAICSAATAA IKHLKTSSWE 310 320 330 340 350 360 RERHQDRAAR VKAILNAAGL PVMSSDTHIV PLFIGDAEKC KQASDLLLEE HGIYIQPINY 370 380 390 400 PTVAKGSERL RITPSPYHDD GLIDQLAEAL LQVWDRLGLP LKQKSLAAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the Bradyrhizobium japonicum gene hemA encoding 5-aminolevulinic acid synthase." McClung C.R., Somerville J.E., Guerinot M.L., Chelm B.K. Gene 54:133-139(1987) [PubMed: 3609750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16751 Genomic DNA. Translation: AAA26216.1. BA000040 Genomic DNA. Translation: BAC46465.1.
PIR	SYZJAL. A27478.
RefSeq	NP_767840.1.
3D structure databases
HSSP	HSSP built from PDB template 1FC4 based on UniProtKB P07912.
ModBase	Search...
Genome annotation databases
GeneID	1049580.
GenomeReviews	Gene locus bll1200 in contig BA000040_GR.
KEGG	bja:bll1200.
NMPDR	fig|224911.1.peg.1200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P08262.
OMA	P08262. AMCETAG.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL1200-MON.
BRENDA	2.3.1.37. 280.
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR004839. Aminotrans_I/II. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_BRAJA

Accession

Primary (citable) accession number: P08262

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	February 28, 2003
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)