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P08262

- HEM1_BRADU

UniProt

P08262 - HEM1_BRADU

Protein

5-aminolevulinate synthase

Gene

hemA

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (28 Feb 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei21 – 211SubstrateBy similarity
    Binding sitei136 – 1361SubstrateBy similarity
    Binding sitei188 – 1881Pyridoxal phosphateBy similarity
    Binding sitei216 – 2161Pyridoxal phosphateBy similarity
    Binding sitei244 – 2441Pyridoxal phosphateBy similarity
    Active sitei247 – 2471By similarity
    Binding sitei276 – 2761Pyridoxal phosphateBy similarity
    Binding sitei277 – 2771Pyridoxal phosphateBy similarity
    Binding sitei362 – 3621SubstrateBy similarity

    GO - Molecular functioni

    1. 5-aminolevulinate synthase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Heme biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-1210-MONOMER.
    UniPathwayiUPA00251; UER00375.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-aminolevulinate synthase (EC:2.3.1.37)
    Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-ALA synthase
    Delta-aminolevulinate synthase
    Gene namesi
    Name:hemA
    Ordered Locus Names:bll1200
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4094095-aminolevulinate synthasePRO_0000163825Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei247 – 2471N6-(pyridoxal phosphate)lysineCurated

    Interactioni

    Protein-protein interaction databases

    STRINGi224911.bll1200.

    Structurei

    3D structure databases

    ProteinModelPortaliP08262.
    SMRiP08262. Positions 2-394.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0156.
    HOGENOMiHOG000221020.
    KOiK00643.
    OrthoDBiEOG6Q8HZD.
    PhylomeDBiP08262.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08262-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYSQFFNSA LDRLHTERRY RVFADLERMA GRFPHAIWHS PKGKRDVVIW    50
    CSNDYLGMGQ HPKVVGAMVE TATRVGTGAG GTRNIAGTHH PLVQLEAELA 100
    DLHGKEAALL FTSGYVSNQT GIATIAKLIP NCLILSDELN HNSMIEGIRQ 150
    SGCERQVFRH NDLADLEALL KAAGANRPKL IACESLYSMD GDVAPLAKIC 200
    DLAEKYNAMT YVDEVHAVGM YGPRGGGIAE RDGVMHRIDI LEGTLAKAFG 250
    CLGGYIAANG QIIDAVRSYA PGFIFTTALP PAICSAATAA IKHLKTSSWE 300
    RERHQDRAAR VKAILNAAGL PVMSSDTHIV PLFIGDAEKC KQASDLLLEE 350
    HGIYIQPINY PTVAKGSERL RITPSPYHDD GLIDQLAEAL LQVWDRLGLP 400
    LKQKSLAAE 409
    Length:409
    Mass (Da):44,574
    Last modified:February 28, 2003 - v2
    Checksum:iB21B011EB9542D66
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti261 – 2611Q → R in AAA26216. (PubMed:3609750)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16751 Genomic DNA. Translation: AAA26216.1.
    BA000040 Genomic DNA. Translation: BAC46465.1.
    PIRiA27478. SYZJAL.
    RefSeqiNP_767840.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC46465; BAC46465; BAC46465.
    GeneIDi1049580.
    KEGGibja:bll1200.
    PATRICi21185942. VBIBraJap65052_1241.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16751 Genomic DNA. Translation: AAA26216.1 .
    BA000040 Genomic DNA. Translation: BAC46465.1 .
    PIRi A27478. SYZJAL.
    RefSeqi NP_767840.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali P08262.
    SMRi P08262. Positions 2-394.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.bll1200.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC46465 ; BAC46465 ; BAC46465 .
    GeneIDi 1049580.
    KEGGi bja:bll1200.
    PATRICi 21185942. VBIBraJap65052_1241.

    Phylogenomic databases

    eggNOGi COG0156.
    HOGENOMi HOG000221020.
    KOi K00643.
    OrthoDBi EOG6Q8HZD.
    PhylomeDBi P08262.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00375 .
    BioCyci BJAP224911:GJEJ-1210-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the Bradyrhizobium japonicum gene hemA encoding 5-aminolevulinic acid synthase."
      McClung C.R., Somerville J.E., Guerinot M.L., Chelm B.K.
      Gene 54:133-139(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiHEM1_BRADU
    AccessioniPrimary (citable) accession number: P08262
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: February 28, 2003
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3