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P08262 (HEM1_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-aminolevulinate synthase

EC=2.3.1.37
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene names
Name:hemA
Ordered Locus Names:bll1200
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactor

Pyridoxal phosphate.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
   Biological processHeme biosynthesis
   LigandPyridoxal phosphate
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function5-aminolevulinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4094095-aminolevulinate synthase
PRO_0000163825

Sites

Active site2471 By similarity
Binding site211Substrate By similarity
Binding site1361Substrate By similarity
Binding site1881Pyridoxal phosphate By similarity
Binding site2161Pyridoxal phosphate By similarity
Binding site2441Pyridoxal phosphate By similarity
Binding site2761Pyridoxal phosphate By similarity
Binding site2771Pyridoxal phosphate By similarity
Binding site3621Substrate By similarity

Amino acid modifications

Modified residue2471N6-(pyridoxal phosphate)lysine Probable

Experimental info

Sequence conflict2611Q → R in AAA26216. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P08262 [UniParc].

Last modified February 28, 2003. Version 2.
Checksum: B21B011EB9542D66

FASTA40944,574
        10         20         30         40         50         60 
MDYSQFFNSA LDRLHTERRY RVFADLERMA GRFPHAIWHS PKGKRDVVIW CSNDYLGMGQ 

        70         80         90        100        110        120 
HPKVVGAMVE TATRVGTGAG GTRNIAGTHH PLVQLEAELA DLHGKEAALL FTSGYVSNQT 

       130        140        150        160        170        180 
GIATIAKLIP NCLILSDELN HNSMIEGIRQ SGCERQVFRH NDLADLEALL KAAGANRPKL 

       190        200        210        220        230        240 
IACESLYSMD GDVAPLAKIC DLAEKYNAMT YVDEVHAVGM YGPRGGGIAE RDGVMHRIDI 

       250        260        270        280        290        300 
LEGTLAKAFG CLGGYIAANG QIIDAVRSYA PGFIFTTALP PAICSAATAA IKHLKTSSWE 

       310        320        330        340        350        360 
RERHQDRAAR VKAILNAAGL PVMSSDTHIV PLFIGDAEKC KQASDLLLEE HGIYIQPINY 

       370        380        390        400 
PTVAKGSERL RITPSPYHDD GLIDQLAEAL LQVWDRLGLP LKQKSLAAE 

« Hide

References

« Hide 'large scale' references
[1]"Structure of the Bradyrhizobium japonicum gene hemA encoding 5-aminolevulinic acid synthase."
McClung C.R., Somerville J.E., Guerinot M.L., Chelm B.K.
Gene 54:133-139(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16751 Genomic DNA. Translation: AAA26216.1.
BA000040 Genomic DNA. Translation: BAC46465.1.
PIRSYZJAL. A27478.
RefSeqNP_767840.1. NC_004463.1.

3D structure databases

ProteinModelPortalP08262.
SMRP08262. Positions 2-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll1200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC46465; BAC46465; BAC46465.
GeneID1049580.
KEGGbja:bll1200.
PATRIC21185942. VBIBraJap65052_1241.

Phylogenomic databases

eggNOGCOG0156.
HOGENOMHOG000221020.
KOK00643.
OMARAEKYIY.
OrthoDBEOG6Q8HZD.
ProtClustDBPRK09064.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-1210-MONOMER.
UniPathwayUPA00251; UER00375.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR01821. 5aminolev_synth. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM1_BRADU
AccessionPrimary (citable) accession number: P08262
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: February 28, 2003
Last modified: December 11, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways