Reviewed, UniProtKB/Swiss-Prot P08252 (CHI1_TOBAC)

Names and origin

Protein names

Recommended name:
    Endochitinase A
      Short name=CHN-A
    EC=3.2.1.14

Gene names

Name:

CHN48

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Vacuole. Note: Vacuolar and protoplast. Ref.3
Developmental stage	Expressed during flower formation. Ref.2
Induction	By ethylene.
Post-translational modification	The 4-hydroxyproline residues are not glycosylated in this plant vacuolar protein.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Vacuole
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Hydroxylation
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 322	299	Endochitinase A		PRO_0000005330
	Propeptide	323 – 329	7	Removed in mature form		PRO_0000005331
Regions
	Domain	24 – 65	42	Chitin-binding type-1
Amino acid modifications
	Modified residue	67	1	4-hydroxyproline; partial Ref.4
	Modified residue	69	1	4-hydroxyproline Ref.4
	Modified residue	71	1	4-hydroxyproline Ref.4
	Modified residue	72	1	4-hydroxyproline Ref.4
	Modified residue	74	1	4-hydroxyproline Ref.4
	Modified residue	75	1	4-hydroxyproline; partial Ref.4
	Disulfide bond	26 ↔ 41		By similarity
	Disulfide bond	35 ↔ 47		By similarity
	Disulfide bond	40 ↔ 54		By similarity
	Disulfide bond	59 ↔ 63		By similarity
	Disulfide bond	101 ↔ 163		By similarity
	Disulfide bond	175 ↔ 183		By similarity
	Disulfide bond	282 ↔ 314		By similarity
Experimental info
	Sequence conflict	73 – 78	6	TPPGGG → HPTRC in AAB23374. Ref.2
	Sequence conflict	263	1	A → S in AAB23374. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08252-1 [UniParc]. Last modified August 1, 1990. Version 2. Checksum: 3EC99D96E6C0114C	FASTA	329	35,156
	10 20 30 40 50 60 MRLCKFTALS SLLFSLLLLS ASAEQCGSQA GGARCPSGLC CSKFGWCGNT NDYCGPGNCQ 70 80 90 100 110 120 SQCPGGPTPT PPTPPGGGDL GSIISSSMFD QMLKHRNDNA CQGKGFYSYN AFINAARSFP 130 140 150 160 170 180 GFGTSGDTTA RKREIAAFFA QTSHETTGGW ATAPDGPYAW GYCWLREQGS PGDYCTPSGQ 190 200 210 220 230 240 WPCAPGRKYF GRGPIQISHN YNYGPCGRAI GVDLLNNPDL VATDPVISFK SALWFWMTPQ 250 260 270 280 290 300 SPKPSCHDVI IGRWQPSAGD RAANRLPGFG VITNIINGGL ECGRGTDSRV QDRIGFYRRY 310 320 CSILGVSPGD NLDCGNQRSF GNGLLVDTM

References

[1]	"Structure of a tobacco endochitinase gene: evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain." Shinshi H., Neuhaus J.-M., Ryals J., Meins F. Jr. Plant Mol. Biol. 14:357-368(1990) [PubMed: 1966383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Havana 425. Tissue: Leaf.
[2]	"Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation." Neale A.D., Wahleithner J.A., Lund M., Bonnett H.T., Kelly A., Meeks-Wagner D.R., Peacock W.J., Dennis E.S. Plant Cell 2:673-684(1990) [PubMed: 2152343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Strain: cv. Samsun. Tissue: Flower bud.
[3]	"A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole." Neuhaus J.-M., Sticher L., Meins F. Jr., Boller T. Proc. Natl. Acad. Sci. U.S.A. 88:10362-10366(1991) [PubMed: 1946457] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Vacuolar chitinases of tobacco: a new class of hydroxyproline-containing proteins." Sticher L., Hofsteenge J., Milani A., Neuhaus J.-M., Meins F. Jr. Science 257:655-657(1992) [PubMed: 1496378] [Abstract] Cited for: HYDROXYLATION AT PRO-67; PRO-69; PRO-71; PRO-72; PRO-74 AND PRO-75, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16938 Genomic DNA. Translation: CAA34812.1. X16939 mRNA. Translation: CAA34813.1. S44869 mRNA. Translation: AAB23374.1.
PIR	S08627.
3D structure databases
HSSP	HSSP built from PDB template 2BAA based on UniProtKB P23951.
SMR	P08252. Positions 24-320.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 298.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI1_TOBAC

Accession

Primary (citable) accession number: P08252
Secondary accession number(s): Q41180

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)