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Protein

Malate dehydrogenase, mitochondrial

Gene

Mdh2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Enzyme regulationi

Enzyme activity is enhanced by acetylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57NADBy similarity1
Binding sitei104SubstrateBy similarity1
Binding sitei110SubstrateBy similarity1
Binding sitei117NADBy similarity1
Binding sitei142SubstrateBy similarity1
Binding sitei176SubstrateBy similarity1
Active sitei200Proton acceptorBy similarity1
Binding sitei251NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 37NADBy similarity7
Nucleotide bindingi140 – 142NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis
R-MMU-71403 Citric acid cycle (TCA cycle)

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:Mdh2
Synonyms:Mor1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97050 Mdh2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 24MitochondrionBy similarityAdd BLAST24
ChainiPRO_000001862925 – 338Malate dehydrogenase, mitochondrialAdd BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33O-linked (GlcNAc) serineBy similarity1
Modified residuei78N6-acetyllysine; alternateCombined sources1
Modified residuei78N6-succinyllysine; alternateCombined sources1
Modified residuei91N6-acetyllysine; alternateCombined sources1
Modified residuei91N6-succinyllysine; alternateCombined sources1
Modified residuei165N6-acetyllysineCombined sources1
Modified residuei185N6-acetyllysine; alternateCombined sources1
Modified residuei185N6-succinyllysine; alternateCombined sources1
Modified residuei203N6-succinyllysineCombined sources1
Modified residuei215N6-acetyllysine; alternateCombined sources1
Modified residuei215N6-succinyllysine; alternateCombined sources1
Modified residuei239N6-acetyllysine; alternateCombined sources1
Modified residuei239N6-malonyllysine; alternateBy similarity1
Modified residuei239N6-succinyllysine; alternateCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei269N6-succinyllysineCombined sources1
Modified residuei296N6-acetyllysine; alternateCombined sources1
Modified residuei296N6-succinyllysine; alternateCombined sources1
Modified residuei301N6-acetyllysine; alternateCombined sources1
Modified residuei301N6-succinyllysine; alternateCombined sources1
Modified residuei307N6-acetyllysine; alternateCombined sources1
Modified residuei307N6-malonyllysine; alternateBy similarity1
Modified residuei307N6-succinyllysine; alternateCombined sources1
Modified residuei309PhosphothreonineCombined sources1
Modified residuei314N6-acetyllysine; alternateCombined sources1
Modified residuei314N6-succinyllysine; alternateCombined sources1
Modified residuei324N6-acetyllysine; alternateCombined sources1
Modified residuei324N6-succinyllysine; alternateCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei328N6-acetyllysine; alternateBy similarity1
Modified residuei328N6-succinyllysine; alternateBy similarity1
Modified residuei329N6-acetyllysine; alternateCombined sources1
Modified residuei329N6-malonyllysine; alternateBy similarity1
Modified residuei335N6-acetyllysine; alternateCombined sources1
Modified residuei335N6-succinyllysine; alternateCombined sources1

Post-translational modificationi

Acetylation is enhanced after treatment either with trichostin A (TCA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation (By similarity). Acetylation of Lys-239 and Lys-314 is observed in liver mitochondria from fasted mice but not from fed mice.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP08249
MaxQBiP08249
PaxDbiP08249
PeptideAtlasiP08249
PRIDEiP08249
TopDownProteomicsiP08249

2D gel databases

REPRODUCTION-2DPAGEiP08249
SWISS-2DPAGEiP08249
UCD-2DPAGEiP08249

PTM databases

CarbonylDBiP08249
iPTMnetiP08249
PhosphoSitePlusiP08249
SwissPalmiP08249

Expressioni

Gene expression databases

BgeeiENSMUSG00000019179
CleanExiMM_MDH2
ExpressionAtlasiP08249 baseline and differential
GenevisibleiP08249 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201467, 5 interactors
IntActiP08249, 9 interactors
MINTiP08249
STRINGi10090.ENSMUSP00000019323

Structurei

3D structure databases

ProteinModelPortaliP08249
SMRiP08249
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1494 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00390000016686
HOGENOMiHOG000213792
HOVERGENiHBG001662
InParanoidiP08249
KOiK00026
OMAiQCTPKVE
OrthoDBiEOG091G0AVO
PhylomeDBiP08249
TreeFamiTF300834

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSALARPAG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV
60 70 80 90 100
SRLTLYDIAH TPGVAADLSH IETRANVKGY LGPEQLPDCL KGCDVVVIPA
110 120 130 140 150
GVPRKPGMTR DDLFNTNATI VATLTAACAQ HCPEAMVCII ANPVNSTIPI
160 170 180 190 200
TAEVFKKHGV YNPNKIFGVT TLDIVRANTF VAELKGLDPA RVNVPVIGGH
210 220 230 240 250
AGKTIIPLIS QCTPKVDFPQ DQLATLTGRI QEAGTEVVKA KAGAGSATLS
260 270 280 290 300
MAYAGARFVF SLVDAMNGKE GVVECSFVQS KETECTYFST PLLLGKKGLE
310 320 330
KNLGIGKITP FEEKMIAEAI PELKASIKKG EDFVKNMK
Length:338
Mass (Da):35,611
Last modified:May 1, 2007 - v3
Checksum:i99D13BB2099C19F1
GO

Sequence cautioni

The sequence BAC24986 differs from that shown. Reason: Frameshift at position 39.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76N → K in AAA39509 (PubMed:3038184).Curated1
Sequence conflicti269K → L in AAA39509 (PubMed:3038184).Curated1
Sequence conflicti269K → L in CAA30274 (PubMed:3379635).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16229 mRNA Translation: AAA39509.1
X07295
, X07296, X07297, X07298, X07299, X07300, X07301 Genomic DNA Translation: CAA30274.1
AK002305 mRNA Translation: BAC24986.1 Frameshift.
AK167809 mRNA Translation: BAE39836.1
AK160553 mRNA Translation: BAE35869.1
AK135162 mRNA Translation: BAE22447.1
BC023482 mRNA Translation: AAH23482.1
DQ402950 mRNA Translation: ABD77283.1
CCDSiCCDS19746.1
PIRiS01350 DEMSMM
RefSeqiNP_032643.2, NM_008617.2
UniGeneiMm.297096

Genome annotation databases

EnsembliENSMUST00000019323; ENSMUSP00000019323; ENSMUSG00000019179
GeneIDi17448
KEGGimmu:17448
UCSCiuc008zyz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMDHM_MOUSE
AccessioniPrimary (citable) accession number: P08249
Secondary accession number(s): Q0QF44, Q8CF79, Q8R1P0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 183 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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