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Protein

Malate dehydrogenase, mitochondrial

Gene

Mdh2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Enzyme regulationi

Enzyme activity is enhanced by acetylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57NADBy similarity1
Binding sitei104Substrate1
Binding sitei110Substrate1
Binding sitei117NADBy similarity1
Binding sitei142Substrate1
Binding sitei176Substrate1
Active sitei200Proton acceptorBy similarity1
Binding sitei251NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 37NADBy similarity7
Nucleotide bindingi140 – 142NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-70263. Gluconeogenesis.
R-MMU-71403. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:Mdh2
Synonyms:Mor1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97050. Mdh2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • membrane Source: ParkinsonsUK-UCL
  • mitochondrial inner membrane Source: MGI
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 24MitochondrionAdd BLAST24
ChainiPRO_000001862925 – 338Malate dehydrogenase, mitochondrialAdd BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33O-linked (GlcNAc)By similarity1
Modified residuei78N6-acetyllysine; alternateCombined sources1
Modified residuei78N6-succinyllysine; alternateCombined sources1
Modified residuei91N6-acetyllysine; alternateCombined sources1
Modified residuei91N6-succinyllysine; alternateCombined sources1
Modified residuei165N6-acetyllysineCombined sources1
Modified residuei185N6-acetyllysine; alternateCombined sources1
Modified residuei185N6-succinyllysine; alternateCombined sources1
Modified residuei203N6-succinyllysineCombined sources1
Modified residuei215N6-acetyllysine; alternateCombined sources1
Modified residuei215N6-succinyllysine; alternateCombined sources1
Modified residuei239N6-acetyllysine; alternateCombined sources1
Modified residuei239N6-malonyllysine; alternateBy similarity1
Modified residuei239N6-succinyllysine; alternateCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei269N6-succinyllysineCombined sources1
Modified residuei296N6-acetyllysine; alternateCombined sources1
Modified residuei296N6-succinyllysine; alternateCombined sources1
Modified residuei301N6-acetyllysine; alternateCombined sources1
Modified residuei301N6-succinyllysine; alternateCombined sources1
Modified residuei307N6-acetyllysine; alternateCombined sources1
Modified residuei307N6-malonyllysine; alternateBy similarity1
Modified residuei307N6-succinyllysine; alternateCombined sources1
Modified residuei309PhosphothreonineCombined sources1
Modified residuei314N6-acetyllysine; alternateCombined sources1
Modified residuei314N6-succinyllysine; alternateCombined sources1
Modified residuei324N6-acetyllysine; alternateCombined sources1
Modified residuei324N6-succinyllysine; alternateCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei328N6-acetyllysine; alternateBy similarity1
Modified residuei328N6-succinyllysine; alternateBy similarity1
Modified residuei329N6-acetyllysine; alternateCombined sources1
Modified residuei329N6-malonyllysine; alternateBy similarity1
Modified residuei335N6-acetyllysine; alternateCombined sources1
Modified residuei335N6-succinyllysine; alternateCombined sources1

Post-translational modificationi

Acetylation is enhanced by up to 67% after treatment either with trichostin A (TCA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60% (By similarity). Acetylation of Lys-239 and Lys-314 is observed in liver mitochondria from fasted mice but not from fed mice.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP08249.
MaxQBiP08249.
PaxDbiP08249.
PeptideAtlasiP08249.
PRIDEiP08249.
TopDownProteomicsiP08249.

2D gel databases

REPRODUCTION-2DPAGEP08249.
SWISS-2DPAGEP08249.
UCD-2DPAGEP08249.

PTM databases

iPTMnetiP08249.
PhosphoSitePlusiP08249.
SwissPalmiP08249.

Expressioni

Gene expression databases

BgeeiENSMUSG00000019179.
CleanExiMM_MDH2.
ExpressionAtlasiP08249. baseline and differential.
GenevisibleiP08249. MM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi201467. 6 interactors.
IntActiP08249. 8 interactors.
MINTiMINT-4101644.
STRINGi10090.ENSMUSP00000019323.

Structurei

3D structure databases

ProteinModelPortaliP08249.
SMRiP08249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1494. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00390000016686.
HOGENOMiHOG000213792.
HOVERGENiHBG001662.
InParanoidiP08249.
KOiK00026.
OMAiVEVKGFA.
OrthoDBiEOG091G0AVO.
PhylomeDBiP08249.
TreeFamiTF300834.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSALARPAG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV
60 70 80 90 100
SRLTLYDIAH TPGVAADLSH IETRANVKGY LGPEQLPDCL KGCDVVVIPA
110 120 130 140 150
GVPRKPGMTR DDLFNTNATI VATLTAACAQ HCPEAMVCII ANPVNSTIPI
160 170 180 190 200
TAEVFKKHGV YNPNKIFGVT TLDIVRANTF VAELKGLDPA RVNVPVIGGH
210 220 230 240 250
AGKTIIPLIS QCTPKVDFPQ DQLATLTGRI QEAGTEVVKA KAGAGSATLS
260 270 280 290 300
MAYAGARFVF SLVDAMNGKE GVVECSFVQS KETECTYFST PLLLGKKGLE
310 320 330
KNLGIGKITP FEEKMIAEAI PELKASIKKG EDFVKNMK
Length:338
Mass (Da):35,611
Last modified:May 1, 2007 - v3
Checksum:i99D13BB2099C19F1
GO

Sequence cautioni

The sequence BAC24986 differs from that shown. Reason: Frameshift at position 39.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76N → K in AAA39509 (PubMed:3038184).Curated1
Sequence conflicti269K → L in AAA39509 (PubMed:3038184).Curated1
Sequence conflicti269K → L in CAA30274 (PubMed:3379635).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16229 mRNA. Translation: AAA39509.1.
X07295
, X07296, X07297, X07298, X07299, X07300, X07301 Genomic DNA. Translation: CAA30274.1.
AK002305 mRNA. Translation: BAC24986.1. Frameshift.
AK167809 mRNA. Translation: BAE39836.1.
AK160553 mRNA. Translation: BAE35869.1.
AK135162 mRNA. Translation: BAE22447.1.
BC023482 mRNA. Translation: AAH23482.1.
DQ402950 mRNA. Translation: ABD77283.1.
CCDSiCCDS19746.1.
PIRiS01350. DEMSMM.
RefSeqiNP_032643.2. NM_008617.2.
UniGeneiMm.297096.

Genome annotation databases

EnsembliENSMUST00000019323; ENSMUSP00000019323; ENSMUSG00000019179.
GeneIDi17448.
KEGGimmu:17448.
UCSCiuc008zyz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16229 mRNA. Translation: AAA39509.1.
X07295
, X07296, X07297, X07298, X07299, X07300, X07301 Genomic DNA. Translation: CAA30274.1.
AK002305 mRNA. Translation: BAC24986.1. Frameshift.
AK167809 mRNA. Translation: BAE39836.1.
AK160553 mRNA. Translation: BAE35869.1.
AK135162 mRNA. Translation: BAE22447.1.
BC023482 mRNA. Translation: AAH23482.1.
DQ402950 mRNA. Translation: ABD77283.1.
CCDSiCCDS19746.1.
PIRiS01350. DEMSMM.
RefSeqiNP_032643.2. NM_008617.2.
UniGeneiMm.297096.

3D structure databases

ProteinModelPortaliP08249.
SMRiP08249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201467. 6 interactors.
IntActiP08249. 8 interactors.
MINTiMINT-4101644.
STRINGi10090.ENSMUSP00000019323.

PTM databases

iPTMnetiP08249.
PhosphoSitePlusiP08249.
SwissPalmiP08249.

2D gel databases

REPRODUCTION-2DPAGEP08249.
SWISS-2DPAGEP08249.
UCD-2DPAGEP08249.

Proteomic databases

EPDiP08249.
MaxQBiP08249.
PaxDbiP08249.
PeptideAtlasiP08249.
PRIDEiP08249.
TopDownProteomicsiP08249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019323; ENSMUSP00000019323; ENSMUSG00000019179.
GeneIDi17448.
KEGGimmu:17448.
UCSCiuc008zyz.1. mouse.

Organism-specific databases

CTDi4191.
MGIiMGI:97050. Mdh2.

Phylogenomic databases

eggNOGiKOG1494. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00390000016686.
HOGENOMiHOG000213792.
HOVERGENiHBG001662.
InParanoidiP08249.
KOiK00026.
OMAiVEVKGFA.
OrthoDBiEOG091G0AVO.
PhylomeDBiP08249.
TreeFamiTF300834.

Enzyme and pathway databases

ReactomeiR-MMU-70263. Gluconeogenesis.
R-MMU-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

ChiTaRSiMdh2. mouse.
PROiP08249.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019179.
CleanExiMM_MDH2.
ExpressionAtlasiP08249. baseline and differential.
GenevisibleiP08249. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDHM_MOUSE
AccessioniPrimary (citable) accession number: P08249
Secondary accession number(s): Q0QF44, Q8CF79, Q8R1P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 173 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.