P08240 (SRPR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle receptor subunit alpha
Short name=SR-alpha

Alternative name(s):
Docking protein alpha
Short name=DP-alpha

Gene names

Name:

SRPR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system.
Subunit structure	Heterodimer with SRPRB. Ref.10
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Thought to be anchored in the membrane through an interaction with SR-beta, which contains a bona fide transmembrane domain.
Sequence similarities	Belongs to the GTP-binding SRP family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Receptor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	SRP-dependent cotranslational protein targeting to membrane Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Traceable author statement. Source: ProtInc signal recognition particle receptor complex Traceable author statement. Source: ProtInc
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro receptor activity Inferred from electronic annotation. Source: UniProtKB-KW signal recognition particle binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 638

638

Signal recognition particle receptor subunit alpha

PRO_0000101213

Regions

Nucleotide binding

425 – 432

8

GTP By similarity

Nucleotide binding

520 – 524

5

GTP By similarity

Nucleotide binding

588 – 591

4

GTP By similarity

Amino acid modifications

Modified residue

261

1

Phosphotyrosine Ref.6

Modified residue

296

1

Phosphoserine Ref.7 Ref.8

Modified residue

297

1

Phosphoserine Ref.7 Ref.8

Modified residue

298

1

Phosphoserine Ref.7 Ref.8

Experimental info

Sequence conflict

81

1

K → R in CAA29608. Ref.1

Sequence conflict

201

1

G → E in CAA29608. Ref.1

Sequence conflict

306 – 307

2

NS → TL in CAA29608. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

638

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Beta strand

12 – 19

8

Turn

20 – 22

3

Helix

28 – 35

8

Helix

37 – 39

3

Beta strand

53 – 59

7

Turn

60 – 63

4

Beta strand

64 – 71

8

Helix

73 – 75

3

Helix

76 – 93

18

Helix

95 – 99

5

Beta strand

100 – 102

3

Helix

104 – 107

4

Helix

114 – 127

14

Sequences

	Sequence		Length	Mass (Da)
	P08240 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 967F943CEE3FA79E	FASTA	638	69,811
	10 20 30 40 50 60 MLDFFTIFSK GGLVLWCFQG VSDSCTGPVN ALIRSVLLQE RGGNNSFTHE ALTLKYKLDN 70 80 90 100 110 120 QFELVFVVGF QKILTLTYVD KLIDDVHRLF RDKYRTEIQQ QSALSLLNGT FDFQNDFLRL 130 140 150 160 170 180 LREAEESSKI RAPTTMKKFE DSEKAKKPVR SMIETRGEKP KEKAKNSKKK GAKKEGSDGP 190 200 210 220 230 240 LATSKPVPAE KSGLPVGPEN GVELSKEELI RRKREEFIQK HGRGMEKSNK STKSDAPKEK 250 260 270 280 290 300 GKKAPRVWEL GGCANKEVLD YSTPTTNGTP EAALSEDINL IRGTGSGGQL QDLDCSSSDD 310 320 330 340 350 360 EGAAQNSTKP SATKGTLGGM FGMLKGLVGS KSLSREDMES VLDKMRDHLI AKNVAADIAV 370 380 390 400 410 420 QLCESVANKL EGKVMGTFST VTSTVKQALQ ESLVQILQPQ RRVDMLRDIM DAQRRQRPYV 430 440 450 460 470 480 VTFCGVNGVG KSTNLAKISF WLLENGFSVL IAACDTFRAG AVEQLRTHTR RLSALHPPEK 490 500 510 520 530 540 HGGRTMVQLF EKGYGKDAAG IAMEAIAFAR NQGFDVVLVD TAGRMQDNAP LMTALAKLIT 550 560 570 580 590 600 VNTPDLVLFV GEALVGNEAV DQLVKFNRAL ADHSMAQTPR LIDGIVLTKF DTIDDKVGAA 610 620 630 ISMTYITSKP IVFVGTGQTY CDLRSLNAKA VVAALMKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete cDNA sequence coding for human docking protein." Hortsch M., Labeit S., Meyer D.I. Nucleic Acids Res. 16:361-362(1988) [PubMed: 3340536] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung, Muscle and Placenta.
[6]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-261, MASS SPECTROMETRY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-296; SER-297 AND SER-298, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-296; SER-297 AND SER-298, MASS SPECTROMETRY.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"The structure of the mammalian signal recognition particle (SRP) receptor as prototype for the interaction of small GTPases with Longin domains." Schlenker O., Hendricks A., Sinning I., Wild K. J. Biol. Chem. 281:8898-8906(2006) [PubMed: 16439358] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 3-176 IN COMPLEX WITH SRPRB, SUBUNIT.
[11]	"Signal recognition particle receptor exposes the ribosomal translocon binding site." Halic M., Gartmann M., Schlenker O., Mielke T., Pool M.R., Sinning I., Beckmann R. Science 312:745-747(2006) [PubMed: 16675701] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.4 ANGSTROMS) OF 3-176 OF SIGNAL RECOGNITION PARTICLE IN COMPLEX WITH THE 80S RIBOSOME AND THE SRP RECEPTOR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X06272 mRNA. Translation: CAA29608.1.
AK312377 mRNA. Translation: BAG35295.1.
AP001318 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW67686.1.
BC001162 mRNA. Translation: AAH01162.1.
BC009110 mRNA. Translation: AAH09110.1.
BC013583 mRNA. Translation: AAH13583.1.

IPI

IPI00385267.

PIR

A29440.

RefSeq

NP_001171313.1. NM_001177842.1.
NP_003130.2. NM_003139.3.

UniGene

Hs.368376.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FH5	X-ray	2.45	A	3-176	[»]
2GO5	electron microscopy	7.40	1	3-176	[»]

ProteinModelPortal

P08240.

SMR

P08240. Positions 1-130, 330-637.

ModBase

Search...

Protein-protein interaction databases

IntAct

P08240. 2 interactions.

MINT

MINT-1404634.

STRING

P08240.

PTM databases

PhosphoSite

P08240.

Polymorphism databases

DMDM

20455516.

Proteomic databases

PRIDE

P08240.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000332118; ENSP00000328023; ENSG00000182934.

GeneID

6734.

KEGG

hsa:6734.

UCSC

uc001qdh.1. human.

Organism-specific databases

CTD

6734.

GeneCards

GC11M126132.

H-InvDB

HIX0010252.

HGNC

HGNC:11307. SRPR.

MIM

182180. gene.

neXtProt

NX_P08240.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG12415.

HOGENOM

HBG562580.

HOVERGEN

HBG047566.

InParanoid

P08240.

OMA

DNQFELV.

OrthoDB

EOG4W0XCP.

PhylomeDB

P08240.

Enzyme and pathway databases

Reactome

REACT_15380. Diabetes pathways.

Gene expression databases

ArrayExpress

P08240.

Bgee

P08240.

CleanEx

HS_SRPR.

Genevestigator

P08240.

GermOnline

ENSG00000182934. Homo sapiens.

Family and domain databases

InterPro

IPR003593. ATPase_AAA+_core.
IPR011012. Longin-like.
IPR007222. Sig_recog_particle_rcpt_asu_N.
IPR000897. Signal_recog_part_SRP54_GTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
[Graphical view]

Gene3D

G3DSA:1.20.120.140. SRP54_helical. 1 hit.

KO

K13431.

Pfam

PF04086. SRP-alpha_N. 1 hit.
PF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]

SUPFAM

SSF64356. Longin_like. 1 hit.
SSF47364. SRP54. 1 hit.

PROSITE

PS00300. SRP54. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

26272.

SOURCE

Search...

Entry information

Entry name

SRPR_HUMAN

Accession

Primary (citable) accession number: P08240
Secondary accession number(s): A6NIB3, B2R5Z8, Q9BVJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	May 2, 2002
Last modified:	January 25, 2012
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.