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P08228

- SODC_MOUSE

UniProt

P08228 - SODC_MOUSE

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Protein

Superoxide dismutase [Cu-Zn]

Gene
Sod1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Catalytic activityi

2 superoxide + 2 H+ = O2 + H2O2.

Cofactori

Binds 1 copper ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Copper; catalytic By similarity
Metal bindingi49 – 491Copper; catalytic By similarity
Metal bindingi64 – 641Copper; catalytic By similarity
Metal bindingi64 – 641Zinc; via pros nitrogen
Metal bindingi72 – 721Zinc; via pros nitrogen
Metal bindingi81 – 811Zinc; via pros nitrogen
Metal bindingi84 – 841Zinc; structural
Metal bindingi121 – 1211Copper; catalytic By similarity

GO - Molecular functioni

  1. chaperone binding Source: UniProtKB
  2. copper ion binding Source: UniProtKB
  3. protein binding Source: IntAct
  4. protein phosphatase 2B binding Source: UniProtKB
  5. superoxide dismutase activity Source: MGI
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. activation of MAPK activity Source: MGI
  2. aging Source: MGI
  3. anterograde axon cargo transport Source: BHF-UCL
  4. auditory receptor cell stereocilium organization Source: MGI
  5. cell aging Source: Ensembl
  6. cellular iron ion homeostasis Source: MGI
  7. embryo implantation Source: MGI
  8. glutathione metabolic process Source: MGI
  9. heart contraction Source: MGI
  10. hydrogen peroxide biosynthetic process Source: MGI
  11. locomotory behavior Source: MGI
  12. muscle cell cellular homeostasis Source: MGI
  13. myeloid cell homeostasis Source: MGI
  14. negative regulation of apoptotic process Source: MGI
  15. negative regulation of cholesterol biosynthetic process Source: UniProtKB
  16. negative regulation of neuron apoptotic process Source: MGI
  17. neurofilament cytoskeleton organization Source: MGI
  18. ovarian follicle development Source: MGI
  19. peripheral nervous system myelin maintenance Source: MGI
  20. positive regulation of catalytic activity Source: UniProtKB
  21. positive regulation of cytokine production Source: UniProtKB
  22. positive regulation of intrinsic apoptotic signaling pathway in response to oxidative stress Source: Ensembl
  23. positive regulation of superoxide anion generation Source: Ensembl
  24. reactive oxygen species metabolic process Source: UniProtKB
  25. regulation of blood pressure Source: MGI
  26. regulation of mitochondrial membrane potential Source: UniProtKB
  27. regulation of multicellular organism growth Source: MGI
  28. regulation of protein kinase activity Source: UniProtKB
  29. regulation of Rac GTPase activity Source: Ensembl
  30. relaxation of vascular smooth muscle Source: MGI
  31. removal of superoxide radicals Source: MGI
  32. response to amphetamine Source: Ensembl
  33. response to axon injury Source: MGI
  34. response to copper ion Source: Ensembl
  35. response to drug Source: MGI
  36. response to ethanol Source: MGI
  37. response to heat Source: MGI
  38. response to hydrogen peroxide Source: MGI
  39. response to nutrient levels Source: Ensembl
  40. response to organic substance Source: UniProtKB
  41. response to oxidative stress Source: MGI
  42. response to reactive oxygen species Source: MGI
  43. response to superoxide Source: MGI
  44. retina homeostasis Source: MGI
  45. retrograde axon cargo transport Source: BHF-UCL
  46. sensory perception of sound Source: MGI
  47. spermatogenesis Source: MGI
  48. superoxide anion generation Source: MGI
  49. superoxide metabolic process Source: MGI
  50. transmission of nerve impulse Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_189141. Detoxification of Reactive Oxygen Species.

Names & Taxonomyi

Protein namesi
Recommended name:
Superoxide dismutase [Cu-Zn] (EC:1.15.1.1)
Gene namesi
Name:Sod1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:98351. Sod1.

Subcellular locationi

Cytoplasm. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytoplasmic vesicle Source: UniProtKB
  3. cytosol Source: UniProtKB
  4. dendrite cytoplasm Source: UniProtKB
  5. extracellular matrix Source: UniProtKB
  6. extracellular space Source: UniProtKB
  7. mitochondrion Source: UniProtKB
  8. neuronal cell body Source: UniProtKB
  9. nucleus Source: UniProtKB
  10. peroxisome Source: Ensembl
  11. plasma membrane Source: Ensembl
  12. protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

40% reduction in hepatic GPX1 activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 154153Superoxide dismutase [Cu-Zn]PRO_0000164062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei4 – 41N6-succinyllysine1 Publication
Lipidationi7 – 71S-palmitoyl cysteine By similarity
Modified residuei10 – 101N6-succinyllysine1 Publication
Disulfide bondi58 ↔ 147 By similarity
Modified residuei92 – 921N6-succinyllysine1 Publication
Modified residuei99 – 991Phosphoserine By similarity
Modified residuei123 – 1231N6-acetyllysine; alternate1 Publication
Modified residuei123 – 1231N6-succinyllysine; alternate1 Publication
Modified residuei137 – 1371N6-acetyllysine; alternate1 Publication
Modified residuei137 – 1371N6-succinyllysine; alternate1 Publication

Post-translational modificationi

Palmitoylation helps nuclear targeting and decreases catalytic activity By similarity.
Succinylation, adjacent to copper catalytic site probably inhibit activity. Desuccinylated by SIRT5, enhancing activity By similarity.

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP08228.
PaxDbiP08228.
PRIDEiP08228.

2D gel databases

DOSAC-COBS-2DPAGEP08228.
REPRODUCTION-2DPAGEIPI00130589.
P08228.
SWISS-2DPAGEP08228.
UCD-2DPAGEP08228.

PTM databases

PhosphoSiteiP08228.

Expressioni

Gene expression databases

BgeeiP08228.
CleanExiMM_SOD1.
GenevestigatoriP08228.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
Rac1P630014EBI-1635090,EBI-413646

Protein-protein interaction databases

BioGridi203387. 9 interactions.
DIPiDIP-48691N.
IntActiP08228. 11 interactions.
MINTiMINT-1869618.
STRINGi10090.ENSMUSP00000023707.

Structurei

Secondary structure

1
154
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108
Beta strandi12 – 143
Beta strandi16 – 2510
Beta strandi30 – 389
Beta strandi41 – 5010
Turni55 – 584
Helixi59 – 613
Beta strandi77 – 793
Beta strandi84 – 907
Beta strandi96 – 10914
Beta strandi116 – 1238
Beta strandi130 – 1323
Helixi133 – 1386
Beta strandi143 – 1497

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GTTX-ray2.40A/B/C/D/E/F2-154[»]
3GTVX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L82-154[»]
3LTVX-ray2.45A/B/C/D/E/F2-154[»]
ProteinModelPortaliP08228.
SMRiP08228. Positions 2-154.

Miscellaneous databases

EvolutionaryTraceiP08228.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2032.
GeneTreeiENSGT00530000063226.
HOGENOMiHOG000263447.
HOVERGENiHBG000062.
InParanoidiP08228.
KOiK04565.
OMAiNDPNAKR.
OrthoDBiEOG776SR4.
PhylomeDBiP08228.
TreeFamiTF105131.

Family and domain databases

Gene3Di2.60.40.200. 1 hit.
InterProiIPR018152. SOD_Cu/Zn_BS.
IPR001424. SOD_Cu_Zn_dom.
[Graphical view]
PfamiPF00080. Sod_Cu. 1 hit.
[Graphical view]
PRINTSiPR00068. CUZNDISMTASE.
SUPFAMiSSF49329. SSF49329. 1 hit.
PROSITEiPS00087. SOD_CU_ZN_1. 1 hit.
PS00332. SOD_CU_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08228-1 [UniParc]FASTAAdd to Basket

« Hide

MAMKAVCVLK GDGPVQGTIH FEQKASGEPV VLSGQITGLT EGQHGFHVHQ    50
YGDNTQGCTS AGPHFNPHSK KHGGPADEER HVGDLGNVTA GKDGVANVSI 100
EDRVISLSGE HSIIGRTMVV HEKQDDLGKG GNEESTKTGN AGSRLACGVI 150
GIAQ 154
Length:154
Mass (Da):15,943
Last modified:January 23, 2007 - v2
Checksum:iCAE548C66043BAC4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021D → H in AAA40121. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06683 mRNA. Translation: CAA29880.1.
M60798
, M60794, M60795, M60796, M60797 Genomic DNA. Translation: AAA40121.1.
M35725 mRNA. Translation: AAA37518.1.
AK020624 mRNA. Translation: BAB32154.1.
AK077284 mRNA. Translation: BAC36730.1.
BC002066 mRNA. Translation: AAH02066.1.
BC048874 mRNA. Translation: AAH48874.1.
BC086886 mRNA. Translation: AAH86886.1.
CCDSiCCDS37395.1.
PIRiJQ0915.
RefSeqiNP_035564.1. NM_011434.1.
UniGeneiMm.276325.
Mm.466779.

Genome annotation databases

EnsembliENSMUST00000023707; ENSMUSP00000023707; ENSMUSG00000022982.
GeneIDi20655.
KEGGimmu:20655.
UCSCiuc007zvz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06683 mRNA. Translation: CAA29880.1 .
M60798
, M60794 , M60795 , M60796 , M60797 Genomic DNA. Translation: AAA40121.1 .
M35725 mRNA. Translation: AAA37518.1 .
AK020624 mRNA. Translation: BAB32154.1 .
AK077284 mRNA. Translation: BAC36730.1 .
BC002066 mRNA. Translation: AAH02066.1 .
BC048874 mRNA. Translation: AAH48874.1 .
BC086886 mRNA. Translation: AAH86886.1 .
CCDSi CCDS37395.1.
PIRi JQ0915.
RefSeqi NP_035564.1. NM_011434.1.
UniGenei Mm.276325.
Mm.466779.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3GTT X-ray 2.40 A/B/C/D/E/F 2-154 [» ]
3GTV X-ray 2.20 A/B/C/D/E/F/G/H/I/J/K/L 82-154 [» ]
3LTV X-ray 2.45 A/B/C/D/E/F 2-154 [» ]
ProteinModelPortali P08228.
SMRi P08228. Positions 2-154.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203387. 9 interactions.
DIPi DIP-48691N.
IntActi P08228. 11 interactions.
MINTi MINT-1869618.
STRINGi 10090.ENSMUSP00000023707.

PTM databases

PhosphoSitei P08228.

2D gel databases

DOSAC-COBS-2DPAGE P08228.
REPRODUCTION-2DPAGE IPI00130589.
P08228.
SWISS-2DPAGE P08228.
UCD-2DPAGE P08228.

Proteomic databases

MaxQBi P08228.
PaxDbi P08228.
PRIDEi P08228.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023707 ; ENSMUSP00000023707 ; ENSMUSG00000022982 .
GeneIDi 20655.
KEGGi mmu:20655.
UCSCi uc007zvz.1. mouse.

Organism-specific databases

CTDi 6647.
MGIi MGI:98351. Sod1.

Phylogenomic databases

eggNOGi COG2032.
GeneTreei ENSGT00530000063226.
HOGENOMi HOG000263447.
HOVERGENi HBG000062.
InParanoidi P08228.
KOi K04565.
OMAi NDPNAKR.
OrthoDBi EOG776SR4.
PhylomeDBi P08228.
TreeFami TF105131.

Enzyme and pathway databases

Reactomei REACT_189141. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

ChiTaRSi SOD1. mouse.
EvolutionaryTracei P08228.
NextBioi 299081.
PROi P08228.
SOURCEi Search...

Gene expression databases

Bgeei P08228.
CleanExi MM_SOD1.
Genevestigatori P08228.

Family and domain databases

Gene3Di 2.60.40.200. 1 hit.
InterProi IPR018152. SOD_Cu/Zn_BS.
IPR001424. SOD_Cu_Zn_dom.
[Graphical view ]
Pfami PF00080. Sod_Cu. 1 hit.
[Graphical view ]
PRINTSi PR00068. CUZNDISMTASE.
SUPFAMi SSF49329. SSF49329. 1 hit.
PROSITEi PS00087. SOD_CU_ZN_1. 1 hit.
PS00332. SOD_CU_ZN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA and deduced amino acid sequence of murine Cu-Zn superoxide dismutase."
    Bewley G.C.
    Nucleic Acids Res. 16:2728-2728(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SWR/J.
    Tissue: Liver.
  2. "Isolation and analysis of the mouse genomic sequence encoding Cu(2+)-Zn2+ superoxide dismutase."
    Benedetto M.T., Anzai Y., Gordon J.W.
    Gene 99:191-195(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Ovary, Urinary bladder and Uterus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver, Mammary gland and Retina.
  5. "Purification of an inhibitor of erythroid progenitor cell cycling and antagonist to interleukin 3 from mouse marrow cell supernatants and its identification as cytosolic superoxide dismutase."
    Pluthero F.G., Shreeve M., Eskinazi D., van der Gaag H., Huang K.S., Hulmes J.D., Blum M., Axelrad A.A.
    J. Cell Biol. 111:1217-1223(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 4-23.
  6. Lubec G., Klug S., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 11-24 AND 104-116, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  7. "Knockout of SOD1 promotes conversion of selenocysteine to dehydroalanine in murine hepatic GPX1 protein."
    Wang S.K., Weaver J.D., Zhang S., Lei X.G.
    Free Radic. Biol. Med. 51:197-204(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Tissue: Liver.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 AND LYS-123, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-4; LYS-10; LYS-92; LYS-123 AND LYS-137, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast and Liver.
  9. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-137, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  10. Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 2-154 IN COMPLEX WITH ZINC.

Entry informationi

Entry nameiSODC_MOUSE
AccessioniPrimary (citable) accession number: P08228
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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