Apolipoprotein E precursor - Mus musculus (Mouse)

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P08226 (APOE_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Apolipoprotein E
Short name=Apo-E

Gene names

Name:

Apoe

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Subcellular location	Secreted.
Tissue specificity	Secreted in plasma.
Post-translational modification	Phosphorylation sites are present in the extracellular medium By similarity.
Sequence similarities	Belongs to the apolipoprotein A1/A4/E family.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Chylomicron HDL Secreted VLDL
Domain	Repeat Signal
Ligand	Heparin-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara aging Inferred from electronic annotation. Source: Compara artery morphogenesis Inferred from genetic interaction PubMed 16230502. Source: MGI cGMP-mediated signaling Inferred from electronic annotation. Source: Compara cellular calcium ion homeostasis Inferred from direct assay PubMed 8185566. Source: MGI cellular response to cholesterol Inferred from electronic annotation. Source: Compara cellular response to growth factor stimulus Inferred from electronic annotation. Source: Compara cellular response to interleukin-1 Inferred from electronic annotation. Source: Compara cholesterol catabolic process Inferred from mutant phenotype PubMed 9826706. Source: MGI cholesterol efflux Inferred from direct assay PubMed 15269218. Source: MGI cholesterol homeostasis Inferred from mutant phenotype PubMed 1423598 PubMed 7798939. Source: MGI chylomicron remnant clearance Inferred from electronic annotation. Source: Compara high-density lipoprotein particle assembly Inferred from electronic annotation. Source: Compara high-density lipoprotein particle clearance Inferred from electronic annotation. Source: Compara high-density lipoprotein particle remodeling Inferred from electronic annotation. Source: Compara induction of apoptosis Inferred from sequence or structural similarity. Source: UniProtKB lipoprotein biosynthetic process Inferred from genetic interaction PubMed 14595002. Source: MGI lipoprotein catabolic process Inferred from mutant phenotype PubMed 17720994. Source: MGI low-density lipoprotein particle remodeling Inferred from mutant phenotype PubMed 15654758. Source: BHF-UCL maintenance of location in cell Inferred from mutant phenotype PubMed 17720994. Source: MGI negative regulation of MAP kinase activity Inferred from electronic annotation. Source: Compara negative regulation of blood vessel endothelial cell migration Inferred from electronic annotation. Source: Compara negative regulation of cholesterol biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of endothelial cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of inflammatory response Inferred from genetic interaction PubMed 21784977. Source: UniProtKB negative regulation of neuron apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of platelet activation Inferred from electronic annotation. Source: Compara nitric oxide mediated signal transduction Inferred from electronic annotation. Source: Compara oligodendrocyte differentiation Inferred from electronic annotation. Source: Compara phospholipid efflux Inferred from electronic annotation. Source: Compara positive regulation of axon extension Inferred from electronic annotation. Source: Compara positive regulation of cGMP biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of cholesterol efflux Inferred from electronic annotation. Source: Compara positive regulation of cholesterol esterification Inferred from mutant phenotype PubMed 15654758. Source: BHF-UCL positive regulation of low-density lipoprotein particle receptor catabolic process Inferred from electronic annotation. Source: Compara positive regulation of membrane protein ectodomain proteolysis Inferred from electronic annotation. Source: Compara positive regulation of nitric-oxide synthase activity Inferred from electronic annotation. Source: Compara receptor-mediated endocytosis Inferred from electronic annotation. Source: Compara regulation of Cdc42 protein signal transduction Inferred from electronic annotation. Source: Compara regulation of gene expression Inferred from mutant phenotype PubMed 17720994. Source: MGI response to dietary excess Inferred from mutant phenotype PubMed 17720994. Source: MGI response to ethanol Inferred from electronic annotation. Source: Compara response to insulin stimulus Inferred from electronic annotation. Source: Compara response to oxidative stress Inferred from mutant phenotype PubMed 8773783. Source: MGI response to retinoic acid Inferred from electronic annotation. Source: Compara reverse cholesterol transport Inferred from electronic annotation. Source: Compara triglyceride metabolic process Inferred from electronic annotation. Source: Compara vasodilation Inferred from mutant phenotype PubMed 9826706. Source: MGI very-low-density lipoprotein particle clearance Inferred from electronic annotation. Source: Compara very-low-density lipoprotein particle remodeling Inferred from mutant phenotype PubMed 15654758. Source: BHF-UCL
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara chylomicron Inferred from electronic annotation. Source: UniProtKB-KW dendrite Inferred from electronic annotation. Source: Compara extrinsic to external side of plasma membrane Inferred from electronic annotation. Source: Compara high-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW intermediate-density lipoprotein particle Inferred from electronic annotation. Source: Compara late endosome Inferred from electronic annotation. Source: Compara low-density lipoprotein particle Inferred from direct assay PubMed 15654758. Source: BHF-UCL neuronal cell body Inferred from electronic annotation. Source: Compara very-low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	antioxidant activity Inferred from electronic annotation. Source: Compara beta-amyloid binding Inferred from sequence or structural similarity. Source: UniProtKB cholesterol transporter activity Inferred from direct assay PubMed 15269218. Source: MGI heparin binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyapatite binding Inferred from electronic annotation. Source: Compara lipoprotein particle binding Inferred from direct assay PubMed 11553788. Source: MGI metal chelating activity Inferred from electronic annotation. Source: Compara phosphatidylcholine-sterol O-acyltransferase activator activity Inferred from mutant phenotype PubMed 15654758. Source: BHF-UCL phospholipid binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

Chain

19 – 311

293

Apolipoprotein E

PRO_0000001990

Regions

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Repeat

Region

176

8 X 22 AA approximate tandem repeats

Region

150 – 160

LDL receptor binding Potential

Region

154 – 157

Heparin-binding By similarity

Region

221 – 228

Heparin-binding By similarity

Experimental info

Sequence conflict

163

E → D in AAA37251. Ref.2

Secondary structure

311

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P08226 [UniParc].

Last modified August 1, 1990. Version 2.
Checksum: 3B36FA897CC34170
Show »

FASTA

311

35,867

        10         20         30         40         50         60 
MKALWAVLLV TLLTGCLAEG EPEVTDQLEW QSNQPWEQAL NRFWDYLRWV QTLSDQVQEE 

        70         80         90        100        110        120 
LQSSQVTQEL TALMEDTMTE VKAYKKELEE QLGPVAEETR ARLGKEVQAA QARLGADMED 

       130        140        150        160        170        180 
LRNRLGQYRN EVHTMLGQST EEIRARLSTH LRKMRKRLMR DAEDLQKRLA VYKAGAREGA 

       190        200        210        220        230        240 
ERGVSAIRER LGPLVEQGRQ RTANLGAGAA QPLRDRAQAF GDRIRGRLEE VGNQARDRLE 

       250        260        270        280        290        300 
EVREHMEEVR SKMEEQTQQI RLQAEIFQAR LKGWFEPIVE DMHRQWANLM EKIQASVATN 

       310 
PIITPVAQEN Q

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of mouse apolipoprotein E gene." Horiuchi K., Tajima S., Menju M., Yamamoto A. J. Biochem. 106:98-103(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evolution of apolipoprotein E: mouse sequence and evidence for an 11-nucleotide ancestral unit." Rajavashisth T.B., Kaptein J.S., Reue K.L., Lusis A.J. Proc. Natl. Acad. Sci. U.S.A. 82:8085-8089(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Neuropathological changes in scrapie and Alzheimer's disease are associated with increased expression of apolipoprotein E and cathepsin D in astrocytes." Diedrich J.F., Minnigan M., Carp R.I., Whitaker J.N., Race R., Frey W. II, Haase A.T. J. Virol. 65:4759-4768(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-311.
[5]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 114-122, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D00466 Genomic DNA. Translation: BAA00361.1.
M12414 mRNA. Translation: AAA37251.1.
BC083351 mRNA. Translation: AAH83351.1.
M73490 mRNA. Translation: AAA37252.1.

IPI

IPI00323571.

PIR

JU0036.

RefSeq

NP_033826.2. NM_009696.3.

UniGene

Mm.305152.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YA9	X-ray	2.09	A	20-200	[»]

ProteinModelPortal

P08226.

SMR

P08226. Positions 19-305.

ModBase

Search...

Protein-protein interaction databases

IntAct

P08226. 4 interactions.

MINT

MINT-242645.

PTM databases

PhosphoSite

P08226.

Proteomic databases

PaxDb

P08226.

PRIDE

P08226.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000003066; ENSMUSP00000003066; ENSMUSG00000002985.
ENSMUST00000173739; ENSMUSP00000133371; ENSMUSG00000002985.
ENSMUST00000174064; ENSMUSP00000133302; ENSMUSG00000002985.
ENSMUST00000174355; ENSMUSP00000134160; ENSMUSG00000002985.

GeneID

11816.

KEGG

mmu:11816.

Organism-specific databases

CTD

348.

MGI

MGI:88057. Apoe.

Phylogenomic databases

eggNOG

NOG44867.

HOGENOM

HOG000034006.

HOVERGEN

HBG010582.

InParanoid

P08226.

K04524.

OMA

EMGSRTR.

OrthoDB

EOG4GB76S.

Gene expression databases

ArrayExpress

P08226.

Bgee

P08226.

CleanEx

MM_APOE.

Genevestigator

P08226.

GermOnline

ENSMUSG00000002985. Mus musculus.

Family and domain databases

InterPro

IPR013326. ApoA.
IPR000074. ApoA1_A4_E.
[Graphical view]

Pfam

PF01442. Apolipoprotein. 1 hit.
[Graphical view]

SUPFAM

SSF47162. SSF47162. 2 hits.

ProtoNet

Search...

Other

ChiTaRS

APOE. mouse.

EvolutionaryTrace

P08226.

NextBio

279703.

SOURCE

Search...

Entry information

Entry name

APOE_MOUSE

Accession

Primary (citable) accession number: P08226

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents