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P08217

- CEL2A_HUMAN

UniProt

P08217 - CEL2A_HUMAN

Protein

Chymotrypsin-like elastase family member 2A

Gene

CELA2A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Acts upon elastin.

    Catalytic activityi

    Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731Charge relay systemBy similarity
    Active sitei121 – 1211Charge relay systemBy similarity
    Active sitei216 – 2161Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine hydrolase activity Source: MGI
    2. serine-type endopeptidase activity Source: ProtInc

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.155.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chymotrypsin-like elastase family member 2A (EC:3.4.21.71)
    Alternative name(s):
    Elastase-2A
    Gene namesi
    Name:CELA2A
    Synonyms:ELA2A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:24609. CELA2A.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. keratohyalin granule Source: MGI

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA165750794.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Add
    BLAST
    Propeptidei17 – 2812Activation peptidePRO_0000027693Add
    BLAST
    Chaini29 – 269241Chymotrypsin-like elastase family member 2APRO_0000027694Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi58 ↔ 74PROSITE-ProRule annotation
    Disulfide bondi155 ↔ 222PROSITE-ProRule annotation
    Disulfide bondi186 ↔ 202PROSITE-ProRule annotation
    Disulfide bondi212 ↔ 243PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP08217.
    PRIDEiP08217.

    PTM databases

    PhosphoSiteiP08217.

    Miscellaneous databases

    PMAP-CutDBP08217.

    Expressioni

    Tissue specificityi

    Pancreas. Not detected in keratinocytes.1 Publication

    Gene expression databases

    BgeeiP08217.
    GenevestigatoriP08217.

    Interactioni

    Subunit structurei

    Interacts with CPA1.1 Publication

    Protein-protein interaction databases

    STRINGi9606.ENSP00000352639.

    Structurei

    3D structure databases

    ProteinModelPortaliP08217.
    SMRiP08217. Positions 29-269.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 267239Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    KOiK01346.
    OMAiAWWGSSV.
    OrthoDBiEOG75B84T.
    PhylomeDBiP08217.
    TreeFamiTF330455.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08217-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRTLLLSTL VAGALSCGDP TYPPYVTRVV GGEEARPNSW PWQVSLQYSS    50
    NGKWYHTCGG SLIANSWVLT AAHCISSSRT YRVGLGRHNL YVAESGSLAV 100
    SVSKIVVHKD WNSNQISKGN DIALLKLANP VSLTDKIQLA CLPPAGTILP 150
    NNYPCYVTGW GRLQTNGAVP DVLQQGRLLV VDYATCSSSA WWGSSVKTSM 200
    ICAGGDGVIS SCNGDSGGPL NCQASDGRWQ VHGIVSFGSR LGCNYYHKPS 250
    VFTRVSNYID WINSVIANN 269
    Length:269
    Mass (Da):28,888
    Last modified:August 1, 1988 - v1
    Checksum:iA2E05143EFF4987C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti202 – 2021C → V in BAA00165. (PubMed:2834346)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti257 – 2571N → S.
    Corresponds to variant rs2303193 [ dbSNP | Ensembl ].
    VAR_051837

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16631 mRNA. Translation: AAA52374.1.
    M16652 mRNA. Translation: AAA52380.1.
    D00236 mRNA. Translation: BAA00165.1.
    AK312198 mRNA. Translation: BAG35131.1.
    AK056678 mRNA. Translation: BAG51782.1.
    AL512883 Genomic DNA. Translation: CAC42421.1.
    CH471167 Genomic DNA. Translation: EAW51727.1.
    BC007031 mRNA. Translation: AAH07031.1.
    CCDSiCCDS157.1.
    PIRiB26823.
    RefSeqiNP_254275.1. NM_033440.2.
    UniGeneiHs.631866.

    Genome annotation databases

    EnsembliENST00000359621; ENSP00000352639; ENSG00000142615.
    GeneIDi63036.
    KEGGihsa:63036.
    UCSCiuc001awk.3. human.

    Polymorphism databases

    DMDMi119255.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16631 mRNA. Translation: AAA52374.1 .
    M16652 mRNA. Translation: AAA52380.1 .
    D00236 mRNA. Translation: BAA00165.1 .
    AK312198 mRNA. Translation: BAG35131.1 .
    AK056678 mRNA. Translation: BAG51782.1 .
    AL512883 Genomic DNA. Translation: CAC42421.1 .
    CH471167 Genomic DNA. Translation: EAW51727.1 .
    BC007031 mRNA. Translation: AAH07031.1 .
    CCDSi CCDS157.1.
    PIRi B26823.
    RefSeqi NP_254275.1. NM_033440.2.
    UniGenei Hs.631866.

    3D structure databases

    ProteinModelPortali P08217.
    SMRi P08217. Positions 29-269.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000352639.

    Chemistry

    BindingDBi P08217.

    Protein family/group databases

    MEROPSi S01.155.

    PTM databases

    PhosphoSitei P08217.

    Polymorphism databases

    DMDMi 119255.

    Proteomic databases

    PaxDbi P08217.
    PRIDEi P08217.

    Protocols and materials databases

    DNASUi 63036.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000359621 ; ENSP00000352639 ; ENSG00000142615 .
    GeneIDi 63036.
    KEGGi hsa:63036.
    UCSCi uc001awk.3. human.

    Organism-specific databases

    CTDi 63036.
    GeneCardsi GC01P015783.
    HGNCi HGNC:24609. CELA2A.
    MIMi 609443. gene.
    neXtProti NX_P08217.
    PharmGKBi PA165750794.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    KOi K01346.
    OMAi AWWGSSV.
    OrthoDBi EOG75B84T.
    PhylomeDBi P08217.
    TreeFami TF330455.

    Miscellaneous databases

    GeneWikii CELA2A.
    GenomeRNAii 63036.
    NextBioi 65535.
    PMAP-CutDB P08217.
    PROi P08217.
    SOURCEi Search...

    Gene expression databases

    Bgeei P08217.
    Genevestigatori P08217.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of human pancreatic elastase 2 determined by sequence analysis of the cloned mRNA."
      Fletcher T.S., Shen W.F., Largman C.
      Biochemistry 26:7256-7261(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization of pancreatic elastase II cDNAs: two elastase II mRNAs are expressed in human pancreas."
      Kawashima I., Tani T., Shimoda K., Takiguchi Y.
      DNA 6:163-172(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Molecular cloning and expression in Escherichia coli of a cDNA encoding human pancreatic elastase 2."
      Shirasu Y., Yoshida H., Matsuki S., Takemura K., Ikeda N., Shimada Y., Ozawa T., Mikayama T., Iijima H., Ishida A., Sato Y., Tamai Y., Tanaka J., Ikenaga H.
      J. Biochem. 102:1555-1563(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pancreas.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas and Prostate.
    5. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas.
    8. "Further studies on the human pancreatic binary complexes involving procarboxypeptidase A."
      Moulard M., Michon T., Kerfelec B., Chapus C.
      FEBS Lett. 261:179-183(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-50, INTERACTION WITH CPA1.
    9. "Human elastase 1: evidence for expression in the skin and the identification of a frequent frameshift polymorphism."
      Talas U., Dunlop J., Khalaf S., Leigh I.M., Kelsell D.P.
      J. Invest. Dermatol. 114:165-170(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCEL2A_HUMAN
    AccessioniPrimary (citable) accession number: P08217
    Secondary accession number(s): B2R5I4, Q14243
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3