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Protein

Malate synthase, glyoxysomal

Gene
N/A
Organism
Cucumis sativus (Cucumber)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei183 – 1831Proton acceptorBy similarity
Active sitei469 – 4691Proton donorBy similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase, glyoxysomal (EC:2.3.3.9)
OrganismiCucumis sativus (Cucumber)
Taxonomic identifieri3659 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 568568Malate synthase, glyoxysomalPRO_0000166868Add
BLAST

Proteomic databases

PRIDEiP08216.

Structurei

3D structure databases

ProteinModelPortaliP08216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi566 – 5683Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLGMYSES GLTKKGSSRG YDVPEGVDIR GRYDEEFAKI LNKEALLFIA
60 70 80 90 100
DLQRTFRNHI KYSMECRREA KRRYNEGGLP GFDPATKYIR DSEWTCAPVP
110 120 130 140 150
PAVADRRVEI TGPVERKMII NALNSGAKVF MADFEDALSP NWENLMRGQI
160 170 180 190 200
NLKDAVDGTI SFHDRVRNRV YKLNDRTAKL FVRPRGWHLP EAHIFIDGEP
210 220 230 240 250
ATGCLVDFGL YFFHNHANFR RSQGQGYGPF FYLPKMEHSR EAKIWNSVFE
260 270 280 290 300
RAEKMAGIER GSIRATVLIE TLPAVFQMNE ILYELRDHSV GLNCGRWDYI
310 320 330 340 350
FSYVKTFQAH PDRLLPDRVL VGMTQHFMRS YSDLLIRTCH RRGVHAMGGM
360 370 380 390 400
AAQIPIRDDP KANEVALELV RKDKLREVKA GHDGTWAAHP GLIPACMEVF
410 420 430 440 450
TNNMGNAPNQ IRSMRRDDAA NLTEEDLLQQ PRGVRTMEGL RLNTRVGIQY
460 470 480 490 500
LAAWLTGAGS VPLYNLAEDA ATAEISRVQN WQWLKYGVEL DGDGLGVRVN
510 520 530 540 550
KELFGRVVEE EMERIEREVG KERFKKGMYK EACKMFTRQC TAPNLDDFLT
560
LDAYNYIVIH HPRELSKL
Length:568
Mass (Da):64,961
Last modified:April 1, 1990 - v2
Checksum:i0C25AB66288ECAA4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti475 – 4751I → G (PubMed:16664899).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15425 Genomic DNA. Translation: CAA33465.1.
M16219 mRNA. Translation: AAA33123.1.
PIRiS07550. SYKVMA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15425 Genomic DNA. Translation: CAA33465.1.
M16219 mRNA. Translation: AAA33123.1.
PIRiS07550. SYKVMA.

3D structure databases

ProteinModelPortaliP08216.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP08216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Long green ridge.
  2. "Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination."
    Smith S.M., Leaver C.J.
    Plant Physiol. 81:762-767(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 475-568.

Entry informationi

Entry nameiMASY_CUCSA
AccessioniPrimary (citable) accession number: P08216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: April 1, 1990
Last modified: January 7, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.