Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P08216

- MASY_CUCSA

UniProt

P08216 - MASY_CUCSA

Protein

Malate synthase, glyoxysomal

Gene
N/A
Organism
Cucumis sativus (Cucumber)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (01 Apr 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei183 – 1831Proton acceptorBy similarity
    Active sitei469 – 4691Proton donorBy similarity

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase, glyoxysomal (EC:2.3.3.9)
    OrganismiCucumis sativus (Cucumber)
    Taxonomic identifieri3659 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 568568Malate synthase, glyoxysomalPRO_0000166868Add
    BLAST

    Proteomic databases

    PRIDEiP08216.

    Structurei

    3D structure databases

    ProteinModelPortaliP08216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi566 – 5683Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the malate synthase family.Curated

    Family and domain databases

    InterProiIPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001363. Malate_synth. 1 hit.
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
    PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08216-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSLGMYSES GLTKKGSSRG YDVPEGVDIR GRYDEEFAKI LNKEALLFIA    50
    DLQRTFRNHI KYSMECRREA KRRYNEGGLP GFDPATKYIR DSEWTCAPVP 100
    PAVADRRVEI TGPVERKMII NALNSGAKVF MADFEDALSP NWENLMRGQI 150
    NLKDAVDGTI SFHDRVRNRV YKLNDRTAKL FVRPRGWHLP EAHIFIDGEP 200
    ATGCLVDFGL YFFHNHANFR RSQGQGYGPF FYLPKMEHSR EAKIWNSVFE 250
    RAEKMAGIER GSIRATVLIE TLPAVFQMNE ILYELRDHSV GLNCGRWDYI 300
    FSYVKTFQAH PDRLLPDRVL VGMTQHFMRS YSDLLIRTCH RRGVHAMGGM 350
    AAQIPIRDDP KANEVALELV RKDKLREVKA GHDGTWAAHP GLIPACMEVF 400
    TNNMGNAPNQ IRSMRRDDAA NLTEEDLLQQ PRGVRTMEGL RLNTRVGIQY 450
    LAAWLTGAGS VPLYNLAEDA ATAEISRVQN WQWLKYGVEL DGDGLGVRVN 500
    KELFGRVVEE EMERIEREVG KERFKKGMYK EACKMFTRQC TAPNLDDFLT 550
    LDAYNYIVIH HPRELSKL 568
    Length:568
    Mass (Da):64,961
    Last modified:April 1, 1990 - v2
    Checksum:i0C25AB66288ECAA4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti475 – 4751I → G(PubMed:16664899)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15425 Genomic DNA. Translation: CAA33465.1.
    M16219 mRNA. Translation: AAA33123.1.
    PIRiS07550. SYKVMA.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15425 Genomic DNA. Translation: CAA33465.1 .
    M16219 mRNA. Translation: AAA33123.1 .
    PIRi S07550. SYKVMA.

    3D structure databases

    ProteinModelPortali P08216.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P08216.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Family and domain databases

    InterProi IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001363. Malate_synth. 1 hit.
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
    PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Long green ridge.
    2. "Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination."
      Smith S.M., Leaver C.J.
      Plant Physiol. 81:762-767(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 475-568.

    Entry informationi

    Entry nameiMASY_CUCSA
    AccessioniPrimary (citable) accession number: P08216
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3