Reviewed, UniProtKB/Swiss-Prot P08216 (MASY_CUCSA)

Names and origin

Protein names	Recommended name:     Malate synthase, glyoxysomal     EC=2.3.3.9
Organism	Cucumis sativus (Cucumber)
Taxonomic identifier	3659 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Transferase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Malate synthase, glyoxysomal		PRO_0000166868
Regions
	Motif	566 – 568	3	Microbody targeting signal Potential
Sites
	Active site	183	1	Proton acceptor By similarity
	Active site	469	1	Proton donor By similarity
Experimental info
	Sequence conflict	475	1	I → G Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P08216-1 [UniParc]. Last modified April 1, 1990. Version 2. Checksum: 0C25AB66288ECAA4	FASTA	568	64,961
	10 20 30 40 50 60 MGSLGMYSES GLTKKGSSRG YDVPEGVDIR GRYDEEFAKI LNKEALLFIA DLQRTFRNHI 70 80 90 100 110 120 KYSMECRREA KRRYNEGGLP GFDPATKYIR DSEWTCAPVP PAVADRRVEI TGPVERKMII 130 140 150 160 170 180 NALNSGAKVF MADFEDALSP NWENLMRGQI NLKDAVDGTI SFHDRVRNRV YKLNDRTAKL 190 200 210 220 230 240 FVRPRGWHLP EAHIFIDGEP ATGCLVDFGL YFFHNHANFR RSQGQGYGPF FYLPKMEHSR 250 260 270 280 290 300 EAKIWNSVFE RAEKMAGIER GSIRATVLIE TLPAVFQMNE ILYELRDHSV GLNCGRWDYI 310 320 330 340 350 360 FSYVKTFQAH PDRLLPDRVL VGMTQHFMRS YSDLLIRTCH RRGVHAMGGM AAQIPIRDDP 370 380 390 400 410 420 KANEVALELV RKDKLREVKA GHDGTWAAHP GLIPACMEVF TNNMGNAPNQ IRSMRRDDAA 430 440 450 460 470 480 NLTEEDLLQQ PRGVRTMEGL RLNTRVGIQY LAAWLTGAGS VPLYNLAEDA ATAEISRVQN 490 500 510 520 530 540 WQWLKYGVEL DGDGLGVRVN KELFGRVVEE EMERIEREVG KERFKKGMYK EACKMFTRQC 550 560 TAPNLDDFLT LDAYNYIVIH HPRELSKL

References

[1]	"The malate synthase gene of cucumber." Graham I.A., Smith L.M., Brown J.W.S., Leaver C.J., Smith S.M. Plant Mol. Biol. 13:673-684(1989) [PubMed: 2491683] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Long green ridge.
[2]	"Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination." Smith S.M., Leaver C.J. Plant Physiol. 81:762-767(1986) [PubMed: 16664899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 475-568.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15425 Genomic DNA. Translation: CAA33465.1. M16219 mRNA. Translation: AAA33123.1.
PIR	SYKVMA. S07550.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.3.9. 1241.
Family and domain databases
InterPro	IPR011076. Malate_synth-like_core. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_CUCSA

Accession

Primary (citable) accession number: P08216

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	April 1, 1990
Last modified:	October 13, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)