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P08216 (MASY_CUCSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase, glyoxysomal

EC=2.3.3.9
OrganismCucumis sativus (Cucumber)
Taxonomic identifier3659 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 568568Malate synthase, glyoxysomal
PRO_0000166868

Regions

Motif566 – 5683Microbody targeting signal Potential

Sites

Active site1831Proton acceptor By similarity
Active site4691Proton donor By similarity

Experimental info

Sequence conflict4751I → G Ref.2

Sequences

Sequence LengthMass (Da)Tools
P08216 [UniParc].

Last modified April 1, 1990. Version 2.
Checksum: 0C25AB66288ECAA4

FASTA56864,961
        10         20         30         40         50         60 
MGSLGMYSES GLTKKGSSRG YDVPEGVDIR GRYDEEFAKI LNKEALLFIA DLQRTFRNHI 

        70         80         90        100        110        120 
KYSMECRREA KRRYNEGGLP GFDPATKYIR DSEWTCAPVP PAVADRRVEI TGPVERKMII 

       130        140        150        160        170        180 
NALNSGAKVF MADFEDALSP NWENLMRGQI NLKDAVDGTI SFHDRVRNRV YKLNDRTAKL 

       190        200        210        220        230        240 
FVRPRGWHLP EAHIFIDGEP ATGCLVDFGL YFFHNHANFR RSQGQGYGPF FYLPKMEHSR 

       250        260        270        280        290        300 
EAKIWNSVFE RAEKMAGIER GSIRATVLIE TLPAVFQMNE ILYELRDHSV GLNCGRWDYI 

       310        320        330        340        350        360 
FSYVKTFQAH PDRLLPDRVL VGMTQHFMRS YSDLLIRTCH RRGVHAMGGM AAQIPIRDDP 

       370        380        390        400        410        420 
KANEVALELV RKDKLREVKA GHDGTWAAHP GLIPACMEVF TNNMGNAPNQ IRSMRRDDAA 

       430        440        450        460        470        480 
NLTEEDLLQQ PRGVRTMEGL RLNTRVGIQY LAAWLTGAGS VPLYNLAEDA ATAEISRVQN 

       490        500        510        520        530        540 
WQWLKYGVEL DGDGLGVRVN KELFGRVVEE EMERIEREVG KERFKKGMYK EACKMFTRQC 

       550        560 
TAPNLDDFLT LDAYNYIVIH HPRELSKL 

« Hide

References

[1]"The malate synthase gene of cucumber."
Graham I.A., Smith L.M., Brown J.W.S., Leaver C.J., Smith S.M.
Plant Mol. Biol. 13:673-684(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Long green ridge.
[2]"Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination."
Smith S.M., Leaver C.J.
Plant Physiol. 81:762-767(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 475-568.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15425 Genomic DNA. Translation: CAA33465.1.
M16219 mRNA. Translation: AAA33123.1.
PIRSYKVMA. S07550.

3D structure databases

ProteinModelPortalP08216.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP08216.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMASY_CUCSA
AccessionPrimary (citable) accession number: P08216
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: April 1, 1990
Last modified: February 19, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways