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Protein

Ribulokinase

Gene

araB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L(or D)-ribulose = ADP + L(or D)-ribulose 5-phosphate.

Pathwayi: L-arabinose degradation via L-ribulose

This protein is involved in step 2 of the subpathway that synthesizes D-xylulose 5-phosphate from L-arabinose (bacterial route).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. L-arabinose isomerase (araA)
  2. Ribulokinase (araB)
  3. L-ribulose-5-phosphate 4-epimerase (araD)
This subpathway is part of the pathway L-arabinose degradation via L-ribulose, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-xylulose 5-phosphate from L-arabinose (bacterial route), the pathway L-arabinose degradation via L-ribulose and in Carbohydrate degradation.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphotransferase activity, alcohol group as acceptor Source: EcoliWiki
  • ribulokinase activity Source: EcoCyc

GO - Biological processi

  • arabinose catabolic process Source: EcoliWiki
  • carbohydrate catabolic process Source: EcoliWiki
  • L-arabinose catabolic process Source: EcoliWiki
  • L-arabinose catabolic process to xylulose 5-phosphate Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Arabinose catabolism, Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:RIBULOKIN-MONOMER.
ECOL316407:JW0062-MONOMER.
MetaCyc:RIBULOKIN-MONOMER.
UniPathwayiUPA00145; UER00566.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulokinase (EC:2.7.1.16)
Gene namesi
Name:araB
Ordered Locus Names:b0063, JW0062
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10053. araB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 566565RibulokinasePRO_0000198358Add
BLAST

Proteomic databases

PaxDbiP08204.
PRIDEiP08204.

Interactioni

Protein-protein interaction databases

IntActiP08204. 1 interaction.
STRINGi511145.b0063.

Structurei

3D structure databases

ProteinModelPortaliP08204.
SMRiP08204. Positions 5-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribulokinase family.Curated

Phylogenomic databases

eggNOGiENOG4105EF1. Bacteria.
COG1069. LUCA.
HOGENOMiHOG000236883.
InParanoidiP08204.
KOiK00853.
OMAiACTMLPI.
OrthoDBiEOG644ZKP.
PhylomeDBiP08204.

Family and domain databases

HAMAPiMF_00520. Ribulokinase.
InterProiIPR018485. Carb_kinase_FGGY_C.
IPR005929. Ribulokinase.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01234. L-ribulokinase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIAIGLDFG SDSVRALAVD CATGEEIATS VEWYPRWQKG QFCDAPNNQF
60 70 80 90 100
RHHPRDYIES MEAALKTVLA ELSVEQRAAV VGIGVDSTGS TPAPIDADGN
110 120 130 140 150
VLALRPEFAE NPNAMFVLWK DHTAVEEAEE ITRLCHAPGN VDYSRYIGGI
160 170 180 190 200
YSSEWFWAKI LHVTRQDSAV AQSAASWIEL CDWVPALLSG TTRPQDIRRG
210 220 230 240 250
RCSAGHKSLW HESWGGLPPA SFFDELDPIL NRHLPSPLFT DTWTADIPVG
260 270 280 290 300
TLCPEWAQRL GLPESVVISG GAFDCHMGAV GAGAQPNALV KVIGTSTCDI
310 320 330 340 350
LIADKQSVGE RAVKGICGQV DGSVVPGFIG LEAGQSAFGD IYAWFGRVLG
360 370 380 390 400
WPLEQLAAQH PELKTQINAS QKQLLPALTE AWAKNPSLDH LPVVLDWFNG
410 420 430 440 450
RRTPNANQRL KGVITDLNLA TDAPLLFGGL IAATAFGARA IMECFTDQGI
460 470 480 490 500
AVNNVMALGG IARKNQVIMQ ACCDVLNRPL QIVASDQCCA LGAAIFAAVA
510 520 530 540 550
AKVHADIPSA QQKMASAVEK TLQPCSEQAQ RFEQLYRRYQ QWAMSAEQHY
560
LPTSAPAQAA QAVATL
Length:566
Mass (Da):61,089
Last modified:January 23, 2007 - v4
Checksum:i4A12010092139D8A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231T → S in AAA23462 (PubMed:3549454).Curated
Sequence conflicti127 – 1282EA → RS in AAA23462 (PubMed:3549454).Curated
Sequence conflicti350 – 3501G → S in AAA23462 (PubMed:3549454).Curated
Sequence conflicti365 – 3651T → A in AAA23462 (PubMed:3549454).Curated
Sequence conflicti403 – 4031T → S in AAA23462 (PubMed:3549454).Curated
Sequence conflicti525 – 5251C → R in AAA23462 (PubMed:3549454).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15263 Genomic DNA. Translation: AAA23462.1.
J01641 Genomic DNA. Translation: AAA23467.1.
U00096 Genomic DNA. Translation: AAC73174.1.
AP009048 Genomic DNA. Translation: BAB96632.2.
K01304 mRNA. Translation: AAA23465.1.
PIRiG64727. KIECRU.
RefSeqiNP_414605.1. NC_000913.3.
WP_000951856.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73174; AAC73174; b0063.
BAB96632; BAB96632; BAB96632.
GeneIDi946017.
KEGGiecj:JW0062.
eco:b0063.
PATRICi32115227. VBIEscCol129921_0065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15263 Genomic DNA. Translation: AAA23462.1.
J01641 Genomic DNA. Translation: AAA23467.1.
U00096 Genomic DNA. Translation: AAC73174.1.
AP009048 Genomic DNA. Translation: BAB96632.2.
K01304 mRNA. Translation: AAA23465.1.
PIRiG64727. KIECRU.
RefSeqiNP_414605.1. NC_000913.3.
WP_000951856.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP08204.
SMRiP08204. Positions 5-539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08204. 1 interaction.
STRINGi511145.b0063.

Proteomic databases

PaxDbiP08204.
PRIDEiP08204.

Protocols and materials databases

DNASUi946017.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73174; AAC73174; b0063.
BAB96632; BAB96632; BAB96632.
GeneIDi946017.
KEGGiecj:JW0062.
eco:b0063.
PATRICi32115227. VBIEscCol129921_0065.

Organism-specific databases

EchoBASEiEB0051.
EcoGeneiEG10053. araB.

Phylogenomic databases

eggNOGiENOG4105EF1. Bacteria.
COG1069. LUCA.
HOGENOMiHOG000236883.
InParanoidiP08204.
KOiK00853.
OMAiACTMLPI.
OrthoDBiEOG644ZKP.
PhylomeDBiP08204.

Enzyme and pathway databases

UniPathwayiUPA00145; UER00566.
BioCyciEcoCyc:RIBULOKIN-MONOMER.
ECOL316407:JW0062-MONOMER.
MetaCyc:RIBULOKIN-MONOMER.

Miscellaneous databases

PROiP08204.

Family and domain databases

HAMAPiMF_00520. Ribulokinase.
InterProiIPR018485. Carb_kinase_FGGY_C.
IPR005929. Ribulokinase.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01234. L-ribulokinase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The organization of the araBAD operon of Escherichia coli."
    Lee N., Gielow W., Martin R., Hamilton E., Fowler A.
    Gene 47:231-244(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Nucleotide sequence of the 5' end of araBAD operon messenger RNA in Escherichia coli B/r."
    Lee N., Carbon J.
    Proc. Natl. Acad. Sci. U.S.A. 74:49-53(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-14, PROTEIN SEQUENCE OF 2-14.
  6. "Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12."
    Smith B.R., Schleif R.
    J. Biol. Chem. 253:6931-6933(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
    Strain: K12.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiARAB_ECOLI
AccessioniPrimary (citable) accession number: P08204
Secondary accession number(s): P78041
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.