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Protein

L-arabinose isomerase

Gene

araA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of L-arabinose to L-ribulose.

Catalytic activityi

L-arabinose = L-ribulose.

Cofactori

Mn2+Note: Binds 1 Mn2+ ion per subunit.

Pathwayi: L-arabinose degradation via L-ribulose

This protein is involved in step 1 of the subpathway that synthesizes D-xylulose 5-phosphate from L-arabinose (bacterial route).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. L-arabinose isomerase (araA)
  2. Ribulokinase (araB)
  3. L-ribulose-5-phosphate 4-epimerase (araD)
This subpathway is part of the pathway L-arabinose degradation via L-ribulose, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-xylulose 5-phosphate from L-arabinose (bacterial route), the pathway L-arabinose degradation via L-ribulose and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi306Manganese1
Metal bindingi333Manganese1
Metal bindingi350Manganese1
Metal bindingi450Manganese1

GO - Molecular functioni

  • L-arabinose isomerase activity Source: EcoCyc
  • manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  • arabinose catabolic process Source: EcoliWiki
  • L-arabinose catabolic process to xylulose 5-phosphate Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Arabinose catabolism, Carbohydrate metabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:ARABISOM-MONOMER.
ECOL316407:JW0061-MONOMER.
MetaCyc:ARABISOM-MONOMER.
BRENDAi5.3.1.4. 2026.
UniPathwayiUPA00145; UER00565.

Names & Taxonomyi

Protein namesi
Recommended name:
L-arabinose isomerase (EC:5.3.1.4)
Gene namesi
Name:araA
Ordered Locus Names:b0062, JW0061
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10052. araA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001983851 – 500L-arabinose isomeraseAdd BLAST500

Proteomic databases

PaxDbiP08202.
PRIDEiP08202.

Interactioni

Subunit structurei

Homohexamer.2 Publications

Protein-protein interaction databases

DIPiDIP-9123N.
IntActiP08202. 4 interactors.
STRINGi511145.b0062.

Structurei

Secondary structure

1500
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 6Combined sources4
Beta strandi8 – 14Combined sources7
Beta strandi17 – 19Combined sources3
Helixi23 – 41Combined sources19
Beta strandi45 – 50Combined sources6
Helixi57 – 69Combined sources13
Beta strandi73 – 79Combined sources7
Helixi87 – 95Combined sources9
Beta strandi100 – 104Combined sources5
Beta strandi107 – 110Combined sources4
Turni113 – 115Combined sources3
Helixi118 – 123Combined sources6
Helixi126 – 139Combined sources14
Beta strandi144 – 149Combined sources6
Helixi154 – 173Combined sources20
Beta strandi177 – 182Combined sources6
Helixi195 – 202Combined sources8
Beta strandi205 – 209Combined sources5
Helixi211 – 219Combined sources9
Helixi223 – 236Combined sources14
Beta strandi237 – 239Combined sources3
Helixi241 – 243Combined sources3
Helixi250 – 270Combined sources21
Beta strandi273 – 276Combined sources4
Helixi291 – 298Combined sources8
Beta strandi302 – 305Combined sources4
Helixi309 – 321Combined sources13
Turni322 – 324Combined sources3
Beta strandi325 – 327Combined sources3
Beta strandi329 – 338Combined sources10
Beta strandi345 – 349Combined sources5
Helixi356 – 358Combined sources3
Beta strandi361 – 363Combined sources3
Beta strandi365 – 368Combined sources4
Turni372 – 375Combined sources4
Beta strandi380 – 383Combined sources4
Beta strandi389 – 398Combined sources10
Beta strandi400 – 412Combined sources13
Beta strandi427 – 433Combined sources7
Helixi435 – 444Combined sources10
Beta strandi449 – 456Combined sources8
Helixi459 – 468Combined sources10
Beta strandi472 – 477Combined sources6
Helixi482 – 496Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AJTX-ray2.60A/B/C1-500[»]
2HXGX-ray2.80A/B/C1-500[»]
4F2DX-ray2.30A/B/C1-500[»]
ProteinModelPortaliP08202.
SMRiP08202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08202.

Family & Domainsi

Sequence similaritiesi

Belongs to the arabinose isomerase family.Curated

Phylogenomic databases

eggNOGiENOG4105CAC. Bacteria.
COG2160. LUCA.
HOGENOMiHOG000252817.
InParanoidiP08202.
KOiK01804.
OMAiLMEDYTY.
PhylomeDBiP08202.

Family and domain databases

HAMAPiMF_00519. Arabinose_Isome. 1 hit.
InterProiIPR024664. Ara_Isoase_C.
IPR004216. Fuc/Ara_isomerase_C.
IPR009015. Fucose_isomerase_N/cen.
IPR003762. Lara_isomerase.
[Graphical view]
PfamiPF11762. Arabinose_Iso_C. 1 hit.
PF02610. Arabinose_Isome. 1 hit.
[Graphical view]
PIRSFiPIRSF001478. L-ara_isomerase. 1 hit.
ProDomiPD018364. Lara_isomerase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50443. SSF50443. 1 hit.
SSF53743. SSF53743. 1 hit.

Sequencei

Sequence statusi: Complete.

P08202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIFDNYEVW FVIGSQHLYG PETLRQVTQH AEHVVNALNT EAKLPCKLVL
60 70 80 90 100
KPLGTTPDEI TAICRDANYD DRCAGLVVWL HTFSPAKMWI NGLTMLNKPL
110 120 130 140 150
LQFHTQFNAA LPWDSIDMDF MNLNQTAHGG REFGFIGARM RQQHAVVTGH
160 170 180 190 200
WQDKQAHERI GSWMRQAVSK QDTRHLKVCR FGDNMREVAV TDGDKVAAQI
210 220 230 240 250
KFGFSVNTWA VGDLVQVVNS ISDGDVNALV DEYESCYTMT PATQIHGKKR
260 270 280 290 300
QNVLEAARIE LGMKRFLEQG GFHAFTTTFE DLHGLKQLPG LAVQRLMQQG
310 320 330 340 350
YGFAGEGDWK TAALLRIMKV MSTGLQGGTS FMEDYTYHFE KGNDLVLGSH
360 370 380 390 400
MLEVCPSIAA EEKPILDVQH LGIGGKDDPA RLIFNTQTGP AIVASLIDLG
410 420 430 440 450
DRYRLLVNCI DTVKTPHSLP KLPVANALWK AQPDLPTASE AWILAGGAHH
460 470 480 490 500
TVFSHALNLN DMRQFAEMHD IEITVIDNDT RLPAFKDALR WNEVYYGFRR
Length:500
Mass (Da):56,074
Last modified:June 27, 2006 - v3
Checksum:iE427A145A3455D36
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72R → P in AAA23463 (PubMed:3549454).Curated1
Sequence conflicti248K → E in AAA23463 (PubMed:3549454).Curated1
Sequence conflicti360A → V in AAA23463 (PubMed:3549454).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15263 Genomic DNA. Translation: AAA23463.1.
U00096 Genomic DNA. Translation: AAC73173.1.
AP009048 Genomic DNA. Translation: BAB96631.2.
X74279 Genomic DNA. Translation: CAA52340.1.
PIRiF64727. ISECAB.
RefSeqiNP_414604.1. NC_000913.3.
WP_000151748.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73173; AAC73173; b0062.
BAB96631; BAB96631; BAB96631.
GeneIDi947511.
KEGGiecj:JW0061.
eco:b0062.
PATRICi32115225. VBIEscCol129921_0064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15263 Genomic DNA. Translation: AAA23463.1.
U00096 Genomic DNA. Translation: AAC73173.1.
AP009048 Genomic DNA. Translation: BAB96631.2.
X74279 Genomic DNA. Translation: CAA52340.1.
PIRiF64727. ISECAB.
RefSeqiNP_414604.1. NC_000913.3.
WP_000151748.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AJTX-ray2.60A/B/C1-500[»]
2HXGX-ray2.80A/B/C1-500[»]
4F2DX-ray2.30A/B/C1-500[»]
ProteinModelPortaliP08202.
SMRiP08202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9123N.
IntActiP08202. 4 interactors.
STRINGi511145.b0062.

Proteomic databases

PaxDbiP08202.
PRIDEiP08202.

Protocols and materials databases

DNASUi947511.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73173; AAC73173; b0062.
BAB96631; BAB96631; BAB96631.
GeneIDi947511.
KEGGiecj:JW0061.
eco:b0062.
PATRICi32115225. VBIEscCol129921_0064.

Organism-specific databases

EchoBASEiEB0050.
EcoGeneiEG10052. araA.

Phylogenomic databases

eggNOGiENOG4105CAC. Bacteria.
COG2160. LUCA.
HOGENOMiHOG000252817.
InParanoidiP08202.
KOiK01804.
OMAiLMEDYTY.
PhylomeDBiP08202.

Enzyme and pathway databases

UniPathwayiUPA00145; UER00565.
BioCyciEcoCyc:ARABISOM-MONOMER.
ECOL316407:JW0061-MONOMER.
MetaCyc:ARABISOM-MONOMER.
BRENDAi5.3.1.4. 2026.

Miscellaneous databases

EvolutionaryTraceiP08202.
PROiP08202.

Family and domain databases

HAMAPiMF_00519. Arabinose_Isome. 1 hit.
InterProiIPR024664. Ara_Isoase_C.
IPR004216. Fuc/Ara_isomerase_C.
IPR009015. Fucose_isomerase_N/cen.
IPR003762. Lara_isomerase.
[Graphical view]
PfamiPF11762. Arabinose_Iso_C. 1 hit.
PF02610. Arabinose_Isome. 1 hit.
[Graphical view]
PIRSFiPIRSF001478. L-ara_isomerase. 1 hit.
ProDomiPD018364. Lara_isomerase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50443. SSF50443. 1 hit.
SSF53743. SSF53743. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARAA_ECOLI
AccessioniPrimary (citable) accession number: P08202
Secondary accession number(s): P78040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 27, 2006
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.