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P08201 (NIRB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrite reductase (NADH) large subunit

EC=1.7.1.15
Gene names
Name:nirB
Ordered Locus Names:b3365, JW3328
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length847 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Ammonia + 3 NAD+ + 2 H2O = nitrite + 3 NADH.

Cofactor

Binds 1 siroheme per subunit.

Binds 1 4Fe-4S cluster per subunit.

FAD.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation).

Subunit structure

Homodimer which associates with NirD.

Sequence similarities

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 847847Nitrite reductase (NADH) large subunit
PRO_0000199962

Regions

Nucleotide binding44 – 7936FAD Potential
Nucleotide binding193 – 22533NAD or NADP Potential

Sites

Metal binding6411Iron-sulfur (4Fe-4S) By similarity
Metal binding6471Iron-sulfur (4Fe-4S) By similarity
Metal binding6811Iron-sulfur (4Fe-4S) By similarity
Metal binding6851Iron (siroheme axial ligand) By similarity
Metal binding6851Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict4421G → A Ref.1
Sequence conflict4421G → A Ref.2
Sequence conflict835 – 84713YERIP…VEDNA → MNVSQ Ref.1
Sequence conflict835 – 84713YERIP…VEDNA → MNVSQ Ref.2

Sequences

Sequence LengthMass (Da)Tools
P08201 [UniParc].

Last modified November 1, 1995. Version 4.
Checksum: 5265AD93FD390EB4

FASTA84793,121
        10         20         30         40         50         60 
MSKVRLAIIG NGMVGHRFIE DLLDKSDAAN FDITVFCEEP RIAYDRVHLS SYFSHHTAEE 

        70         80         90        100        110        120 
LSLVREGFYE KHGIKVLVGE RAITINRQEK VIHSSAGRTV FYDKLIMATG SYPWIPPIKG 

       130        140        150        160        170        180 
SDTQDCFVYR TIEDLNAIES CARRSKRGAV VGGGLLGLEA AGALKNLGIE THVIEFAPML 

       190        200        210        220        230        240 
MAEQLDQMGG EQLRRKIESM GVRVHTSKNT LEIVQEGVEA RKTMRFADGS ELEVDFIVFS 

       250        260        270        280        290        300 
TGIRPRDKLA TQCGLDVAPR GGIVINDSCQ TSDPDIYAIG ECASWNNRVF GLVAPGYKMA 

       310        320        330        340        350        360 
QVAVDHILGS ENAFEGADLS AKLKLLGVDV GGIGDAHGRT PGARSYVYLD ESKEIYKRLI 

       370        380        390        400        410        420 
VSEDNKTLLG AVLVGDTSDY GNLLQLVLNA IELPENPDSL ILPAHSGSGK PSIGVDKLPD 

       430        440        450        460        470        480 
SAQICSCFDV TKGDLIAAIN KGCHTVAALK AETKAGTGCG GCIPLVTQVL NAELAKQGIE 

       490        500        510        520        530        540 
VNNNLCEHFA YSRQELFHLI RVEGIKTFEE LLAKHGKGYG CEVCKPTVGS LLASCWNEYI 

       550        560        570        580        590        600 
LKPEHTPLQD SNDNFLANIQ KDGTYSVIPR SPGGEITPEG LMAVGRIARE FNLYTKITGS 

       610        620        630        640        650        660 
QRLAMFGAQK DDLPEIWRQL IEAGFETGHA YAKALRMAKT CVGSTWCRYG VGDSVGLGVE 

       670        680        690        700        710        720 
LENRYKGIRT PHKMKFGVSG CTRECSEAQG KDVGIIATEK GWNLYVCGNG GMKPRHADLL 

       730        740        750        760        770        780 
AADIDRETLI KYLDRFMMFY IRTADKLTRT APWLENLEGG IDYLKAVIID DKLGLNAHLE 

       790        800        810        820        830        840 
EEMARLREAV LCEWTETVNT PSAQTRFKHF INSDKRDPNV QMVPEREQHR PATPYERIPV 


TLVEDNA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of binding sequences for the Escherichia coli transcription activators, FNR and CRP: location of bases involved in discrimination between FNR and CRP."
Bell A.I., Gaston K.L., Cole J.A., Busby S.J.W.
Nucleic Acids Res. 17:3865-3874(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome."
Peakman T., Crouzet J., Mayaux J.F., Busby S.J.W., Mohan S., Harborne N., Wootton J., Nicolson R., Cole J.A.
Eur. J. Biochem. 191:315-323(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Location and sequence of the promoter of the gene for the NADH-dependent nitrite reductase of Escherichia coli and its regulation by oxygen, the Fnr protein and nitrite."
Jayaraman P.S., Peakman T.C., Busby S.J.W., Quincey R.V., Cole J.A.
J. Mol. Biol. 196:781-788(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14202 Genomic DNA. Translation: CAA32416.1.
U18997 Genomic DNA. Translation: AAA58162.1.
U00096 Genomic DNA. Translation: AAC76390.1.
AP009048 Genomic DNA. Translation: BAE77925.1.
PIRH65130.
RefSeqNP_417824.1. NC_000913.3.
YP_492066.1. NC_007779.1.

3D structure databases

ProteinModelPortalP08201.
SMRP08201. Positions 3-400.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP08201. 1 interaction.
STRING511145.b3365.

Proteomic databases

PaxDbP08201.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76390; AAC76390; b3365.
BAE77925; BAE77925; BAE77925.
GeneID12932093.
947868.
KEGGecj:Y75_p3810.
eco:b3365.
PATRIC32122164. VBIEscCol129921_3459.

Organism-specific databases

EchoBASEEB0647.
EcoGeneEG10653. nirB.

Phylogenomic databases

eggNOGCOG1251.
HOGENOMHOG000196164.
KOK00362.
OMAIRSPHKI.
OrthoDBEOG6G4VT5.
PhylomeDBP08201.

Enzyme and pathway databases

BioCycEcoCyc:NIRB-MONOMER.
ECOL316407:JW3328-MONOMER.
MetaCyc:NIRB-MONOMER.
UniPathwayUPA00653.

Gene expression databases

GenevestigatorP08201.

Family and domain databases

InterProIPR007419. BFD-like_2Fe2S-bd_dom.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR012744. Nitri_red_NirB.
IPR017121. Nitrite_Rdtase_lsu.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF04324. Fer2_BFD. 1 hit.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PIRSFPIRSF037149. NirB. 1 hit.
PRINTSPR00368. FADPNR.
PR00397. SIROHAEM.
SUPFAMSSF55124. SSF55124. 1 hit.
TIGRFAMsTIGR02374. nitri_red_nirB. 1 hit.
PROSITEPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP08201.

Entry information

Entry nameNIRB_ECOLI
AccessionPrimary (citable) accession number: P08201
Secondary accession number(s): Q2M731
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 138 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene