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Protein

Nitrite reductase (NADH) large subunit

Gene

nirB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Ammonia + 3 NAD+ + 2 H2O = nitrite + 3 NADH.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi641Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi647Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi681Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi685Iron (siroheme axial ligand)By similarity1
Metal bindingi685Iron-sulfur (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 79FADSequence analysisAdd BLAST36
Nucleotide bindingi193 – 225NAD or NADPSequence analysisAdd BLAST33

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • flavin adenine dinucleotide binding Source: EcoCyc
  • heme binding Source: EcoCyc
  • iron-sulfur cluster binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • NADP binding Source: EcoCyc
  • nitrite reductase [NAD(P)H] activity Source: EcoCyc

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • nitrate assimilation Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
Ligand4Fe-4S, FAD, Flavoprotein, Heme, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:NIRB-MONOMER
MetaCyc:NIRB-MONOMER
UniPathwayiUPA00653

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase (NADH) large subunit (EC:1.7.1.15)
Gene namesi
Name:nirB
Ordered Locus Names:b3365, JW3328
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10653 nirB

Subcellular locationi

GO - Cellular componenti

  • nitrite reductase complex [NAD(P)H] Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001999621 – 847Nitrite reductase (NADH) large subunitAdd BLAST847

Proteomic databases

PaxDbiP08201
PRIDEiP08201

Interactioni

Subunit structurei

Homodimer which associates with NirD.

Protein-protein interaction databases

BioGridi4261087, 21 interactors
IntActiP08201, 1 interactor
STRINGi316385.ECDH10B_3541

Structurei

3D structure databases

ProteinModelPortaliP08201
SMRiP08201
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QZF Bacteria
COG1251 LUCA
HOGENOMiHOG000196164
InParanoidiP08201
KOiK00362
OMAiMYYIRTA
PhylomeDBiP08201

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR007419 BFD-like_2Fe2S-bd_dom
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR005117 NiRdtase/SiRdtase_haem-b_fer
IPR036136 Nit/Sulf_reduc_fer_like_dom_sf
IPR012744 Nitri_red_NirB
IPR017121 Nitrite_Rdtase_lsu
IPR006067 NO2/SO3_Rdtase_4Fe4S_dom
IPR006066 NO2/SO3_Rdtase_FeS/sirohaem_BS
PfamiView protein in Pfam
PF04324 Fer2_BFD, 1 hit
PF01077 NIR_SIR, 1 hit
PF03460 NIR_SIR_ferr, 1 hit
PF07992 Pyr_redox_2, 1 hit
PIRSFiPIRSF037149 NirB, 1 hit
PRINTSiPR00397 SIROHAEM
SUPFAMiSSF51905 SSF51905, 2 hits
SSF55124 SSF55124, 1 hit
TIGRFAMsiTIGR02374 nitri_red_nirB, 1 hit
PROSITEiView protein in PROSITE
PS00365 NIR_SIR, 1 hit

Sequencei

Sequence statusi: Complete.

P08201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVRLAIIG NGMVGHRFIE DLLDKSDAAN FDITVFCEEP RIAYDRVHLS
60 70 80 90 100
SYFSHHTAEE LSLVREGFYE KHGIKVLVGE RAITINRQEK VIHSSAGRTV
110 120 130 140 150
FYDKLIMATG SYPWIPPIKG SDTQDCFVYR TIEDLNAIES CARRSKRGAV
160 170 180 190 200
VGGGLLGLEA AGALKNLGIE THVIEFAPML MAEQLDQMGG EQLRRKIESM
210 220 230 240 250
GVRVHTSKNT LEIVQEGVEA RKTMRFADGS ELEVDFIVFS TGIRPRDKLA
260 270 280 290 300
TQCGLDVAPR GGIVINDSCQ TSDPDIYAIG ECASWNNRVF GLVAPGYKMA
310 320 330 340 350
QVAVDHILGS ENAFEGADLS AKLKLLGVDV GGIGDAHGRT PGARSYVYLD
360 370 380 390 400
ESKEIYKRLI VSEDNKTLLG AVLVGDTSDY GNLLQLVLNA IELPENPDSL
410 420 430 440 450
ILPAHSGSGK PSIGVDKLPD SAQICSCFDV TKGDLIAAIN KGCHTVAALK
460 470 480 490 500
AETKAGTGCG GCIPLVTQVL NAELAKQGIE VNNNLCEHFA YSRQELFHLI
510 520 530 540 550
RVEGIKTFEE LLAKHGKGYG CEVCKPTVGS LLASCWNEYI LKPEHTPLQD
560 570 580 590 600
SNDNFLANIQ KDGTYSVIPR SPGGEITPEG LMAVGRIARE FNLYTKITGS
610 620 630 640 650
QRLAMFGAQK DDLPEIWRQL IEAGFETGHA YAKALRMAKT CVGSTWCRYG
660 670 680 690 700
VGDSVGLGVE LENRYKGIRT PHKMKFGVSG CTRECSEAQG KDVGIIATEK
710 720 730 740 750
GWNLYVCGNG GMKPRHADLL AADIDRETLI KYLDRFMMFY IRTADKLTRT
760 770 780 790 800
APWLENLEGG IDYLKAVIID DKLGLNAHLE EEMARLREAV LCEWTETVNT
810 820 830 840
PSAQTRFKHF INSDKRDPNV QMVPEREQHR PATPYERIPV TLVEDNA
Length:847
Mass (Da):93,121
Last modified:November 1, 1995 - v4
Checksum:i5265AD93FD390EB4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti442G → A (PubMed:2543955).Curated1
Sequence conflicti442G → A (PubMed:2200672).Curated1
Sequence conflicti835 – 847YERIP…VEDNA → MNVSQ (PubMed:2543955).CuratedAdd BLAST13
Sequence conflicti835 – 847YERIP…VEDNA → MNVSQ (PubMed:2200672).CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14202 Genomic DNA Translation: CAA32416.1
U18997 Genomic DNA Translation: AAA58162.1
U00096 Genomic DNA Translation: AAC76390.1
AP009048 Genomic DNA Translation: BAE77925.1
PIRiH65130
RefSeqiNP_417824.1, NC_000913.3
WP_000049208.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76390; AAC76390; b3365
BAE77925; BAE77925; BAE77925
GeneIDi947868
KEGGiecj:JW3328
eco:b3365
PATRICifig|1411691.4.peg.3364

Entry informationi

Entry nameiNIRB_ECOLI
AccessioniPrimary (citable) accession number: P08201
Secondary accession number(s): Q2M731
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 163 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health