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P08201

- NIRB_ECOLI

UniProt

P08201 - NIRB_ECOLI

Protein

Nitrite reductase (NADH) large subunit

Gene

nirB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 4 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Ammonia + 3 NAD+ + 2 H2O = nitrite + 3 NADH.

    Cofactori

    Binds 1 siroheme per subunit.
    Binds 1 4Fe-4S cluster per subunit.
    FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi641 – 6411Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi647 – 6471Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi681 – 6811Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi685 – 6851Iron (siroheme axial ligand)By similarity
    Metal bindingi685 – 6851Iron-sulfur (4Fe-4S)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi44 – 7936FADSequence AnalysisAdd
    BLAST
    Nucleotide bindingi193 – 22533NAD or NADPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. flavin adenine dinucleotide binding Source: EcoCyc
    3. heme binding Source: EcoCyc
    4. iron-sulfur cluster binding Source: EcoCyc
    5. metal ion binding Source: UniProtKB-KW
    6. NADP binding Source: EcoCyc
    7. nitrite reductase [NAD(P)H] activity Source: EcoCyc

    GO - Biological processi

    1. anaerobic respiration Source: EcoCyc
    2. nitrate assimilation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrate assimilation

    Keywords - Ligandi

    4Fe-4S, FAD, Flavoprotein, Heme, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:NIRB-MONOMER.
    ECOL316407:JW3328-MONOMER.
    MetaCyc:NIRB-MONOMER.
    UniPathwayiUPA00653.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrite reductase (NADH) large subunit (EC:1.7.1.15)
    Gene namesi
    Name:nirB
    Ordered Locus Names:b3365, JW3328
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10653. nirB.

    Subcellular locationi

    GO - Cellular componenti

    1. nitrite reductase complex [NAD(P)H] Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 847847Nitrite reductase (NADH) large subunitPRO_0000199962Add
    BLAST

    Proteomic databases

    PaxDbiP08201.

    Expressioni

    Gene expression databases

    GenevestigatoriP08201.

    Interactioni

    Subunit structurei

    Homodimer which associates with NirD.

    Protein-protein interaction databases

    IntActiP08201. 1 interaction.
    STRINGi511145.b3365.

    Structurei

    3D structure databases

    ProteinModelPortaliP08201.
    SMRiP08201. Positions 3-400.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1251.
    HOGENOMiHOG000196164.
    KOiK00362.
    OMAiIRSPHKI.
    OrthoDBiEOG6G4VT5.
    PhylomeDBiP08201.

    Family and domain databases

    InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR005117. NiRdtase/SiRdtase_haem-b_fer.
    IPR012744. Nitri_red_NirB.
    IPR017121. Nitrite_Rdtase_lsu.
    IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
    IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view]
    PfamiPF04324. Fer2_BFD. 1 hit.
    PF01077. NIR_SIR. 1 hit.
    PF03460. NIR_SIR_ferr. 1 hit.
    PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037149. NirB. 1 hit.
    PRINTSiPR00368. FADPNR.
    PR00397. SIROHAEM.
    SUPFAMiSSF55124. SSF55124. 1 hit.
    TIGRFAMsiTIGR02374. nitri_red_nirB. 1 hit.
    PROSITEiPS00365. NIR_SIR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08201-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKVRLAIIG NGMVGHRFIE DLLDKSDAAN FDITVFCEEP RIAYDRVHLS    50
    SYFSHHTAEE LSLVREGFYE KHGIKVLVGE RAITINRQEK VIHSSAGRTV 100
    FYDKLIMATG SYPWIPPIKG SDTQDCFVYR TIEDLNAIES CARRSKRGAV 150
    VGGGLLGLEA AGALKNLGIE THVIEFAPML MAEQLDQMGG EQLRRKIESM 200
    GVRVHTSKNT LEIVQEGVEA RKTMRFADGS ELEVDFIVFS TGIRPRDKLA 250
    TQCGLDVAPR GGIVINDSCQ TSDPDIYAIG ECASWNNRVF GLVAPGYKMA 300
    QVAVDHILGS ENAFEGADLS AKLKLLGVDV GGIGDAHGRT PGARSYVYLD 350
    ESKEIYKRLI VSEDNKTLLG AVLVGDTSDY GNLLQLVLNA IELPENPDSL 400
    ILPAHSGSGK PSIGVDKLPD SAQICSCFDV TKGDLIAAIN KGCHTVAALK 450
    AETKAGTGCG GCIPLVTQVL NAELAKQGIE VNNNLCEHFA YSRQELFHLI 500
    RVEGIKTFEE LLAKHGKGYG CEVCKPTVGS LLASCWNEYI LKPEHTPLQD 550
    SNDNFLANIQ KDGTYSVIPR SPGGEITPEG LMAVGRIARE FNLYTKITGS 600
    QRLAMFGAQK DDLPEIWRQL IEAGFETGHA YAKALRMAKT CVGSTWCRYG 650
    VGDSVGLGVE LENRYKGIRT PHKMKFGVSG CTRECSEAQG KDVGIIATEK 700
    GWNLYVCGNG GMKPRHADLL AADIDRETLI KYLDRFMMFY IRTADKLTRT 750
    APWLENLEGG IDYLKAVIID DKLGLNAHLE EEMARLREAV LCEWTETVNT 800
    PSAQTRFKHF INSDKRDPNV QMVPEREQHR PATPYERIPV TLVEDNA 847
    Length:847
    Mass (Da):93,121
    Last modified:November 1, 1995 - v4
    Checksum:i5265AD93FD390EB4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti442 – 4421G → A(PubMed:2543955)Curated
    Sequence conflicti442 – 4421G → A(PubMed:2200672)Curated
    Sequence conflicti835 – 84713YERIP…VEDNA → MNVSQ(PubMed:2543955)CuratedAdd
    BLAST
    Sequence conflicti835 – 84713YERIP…VEDNA → MNVSQ(PubMed:2200672)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14202 Genomic DNA. Translation: CAA32416.1.
    U18997 Genomic DNA. Translation: AAA58162.1.
    U00096 Genomic DNA. Translation: AAC76390.1.
    AP009048 Genomic DNA. Translation: BAE77925.1.
    PIRiH65130.
    RefSeqiNP_417824.1. NC_000913.3.
    YP_492066.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76390; AAC76390; b3365.
    BAE77925; BAE77925; BAE77925.
    GeneIDi12932093.
    947868.
    KEGGiecj:Y75_p3810.
    eco:b3365.
    PATRICi32122164. VBIEscCol129921_3459.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14202 Genomic DNA. Translation: CAA32416.1 .
    U18997 Genomic DNA. Translation: AAA58162.1 .
    U00096 Genomic DNA. Translation: AAC76390.1 .
    AP009048 Genomic DNA. Translation: BAE77925.1 .
    PIRi H65130.
    RefSeqi NP_417824.1. NC_000913.3.
    YP_492066.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P08201.
    SMRi P08201. Positions 3-400.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08201. 1 interaction.
    STRINGi 511145.b3365.

    Proteomic databases

    PaxDbi P08201.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76390 ; AAC76390 ; b3365 .
    BAE77925 ; BAE77925 ; BAE77925 .
    GeneIDi 12932093.
    947868.
    KEGGi ecj:Y75_p3810.
    eco:b3365.
    PATRICi 32122164. VBIEscCol129921_3459.

    Organism-specific databases

    EchoBASEi EB0647.
    EcoGenei EG10653. nirB.

    Phylogenomic databases

    eggNOGi COG1251.
    HOGENOMi HOG000196164.
    KOi K00362.
    OMAi IRSPHKI.
    OrthoDBi EOG6G4VT5.
    PhylomeDBi P08201.

    Enzyme and pathway databases

    UniPathwayi UPA00653 .
    BioCyci EcoCyc:NIRB-MONOMER.
    ECOL316407:JW3328-MONOMER.
    MetaCyc:NIRB-MONOMER.

    Miscellaneous databases

    PROi P08201.

    Gene expression databases

    Genevestigatori P08201.

    Family and domain databases

    InterProi IPR007419. BFD-like_2Fe2S-bd_dom.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR005117. NiRdtase/SiRdtase_haem-b_fer.
    IPR012744. Nitri_red_NirB.
    IPR017121. Nitrite_Rdtase_lsu.
    IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
    IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF04324. Fer2_BFD. 1 hit.
    PF01077. NIR_SIR. 1 hit.
    PF03460. NIR_SIR_ferr. 1 hit.
    PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037149. NirB. 1 hit.
    PRINTSi PR00368. FADPNR.
    PR00397. SIROHAEM.
    SUPFAMi SSF55124. SSF55124. 1 hit.
    TIGRFAMsi TIGR02374. nitri_red_nirB. 1 hit.
    PROSITEi PS00365. NIR_SIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of binding sequences for the Escherichia coli transcription activators, FNR and CRP: location of bases involved in discrimination between FNR and CRP."
      Bell A.I., Gaston K.L., Cole J.A., Busby S.J.W.
      Nucleic Acids Res. 17:3865-3874(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome."
      Peakman T., Crouzet J., Mayaux J.F., Busby S.J.W., Mohan S., Harborne N., Wootton J., Nicolson R., Cole J.A.
      Eur. J. Biochem. 191:315-323(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Location and sequence of the promoter of the gene for the NADH-dependent nitrite reductase of Escherichia coli and its regulation by oxygen, the Fnr protein and nitrite."
      Jayaraman P.S., Peakman T.C., Busby S.J.W., Quincey R.V., Cole J.A.
      J. Mol. Biol. 196:781-788(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.

    Entry informationi

    Entry nameiNIRB_ECOLI
    AccessioniPrimary (citable) accession number: P08201
    Secondary accession number(s): Q2M731
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 139 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3