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P08196 (PSY1_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phytoene synthase 1, chloroplastic

EC=2.5.1.32
Alternative name(s):
Fruit-ripening-specific protein pTOM5
Gene names
Name:PSY1
Synonyms:GTOM5, PTOM5, TOM5
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reaction from prephytoene diphosphate to phytoene.

Catalytic activity

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathway

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Subunit structure

Monomer By similarity.

Subcellular location

Plastidchloroplast.

Developmental stage

In seedlings and in late stages of fruit ripening.

Induction

By fruit ripening.

Sequence similarities

Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
   Biological processCarotenoid biosynthesis
Isoprene biosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionTransferase
   Technical termComplete proteome
Multifunctional enzyme
Reference proteome
Gene Ontology (GO)
   Biological_processcarotenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplastoglobule

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functiongeranylgeranyl-diphosphate geranylgeranyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 129129Chloroplast Potential
Chain130 – 412283Phytoene synthase 1, chloroplastic
PRO_0000029856

Experimental info

Mutagenesis388 – 41225KKLIA…ASLQR → NMLKDFFSNFKGSKRGSNAT TTLVG: Lack of carotenoids in fruits.
Sequence conflict389 – 40719KLIAL…PPTKT → QVDCITYCICKISCASYKN in CAA68575. Ref.3
Sequence conflict389 – 40517KLIAL…LVPPT → QVDCITYCICKISCASY in CAA47625. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P08196 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: DBED05945D6F4C9A

FASTA41246,615
        10         20         30         40         50         60 
MSVALLWVVS PCDVSNGTSF MESVREGNRF FDSSRHRNLV SNERINRGGG KQTNNGRKFS 

        70         80         90        100        110        120 
VRSAILATPS GERTMTSEQM VYDVVLRQAA LVKRQLRSTN ELEVKPDIPI PGNLGLLSEA 

       130        140        150        160        170        180 
YDRCGEVCAE YAKTFNLGTM LMTPERRRAI WAIYVWCRRT DELVDGPNAS YITPAALDRW 

       190        200        210        220        230        240 
ENRLEDVFNG RPFDMLDGAL SDTVSNFPVD IQPFRDMIEG MRMDLRKSRY KNFDELYLYC 

       250        260        270        280        290        300 
YYVAGTVGLM SVPIMGIAPE SKATTESVYN AALALGIANQ LTNILRDVGE DARRGRVYLP 

       310        320        330        340        350        360 
QDELAQAGLS DEDIFAGRVT DKWRIFMKKQ IHRARKFFDE AEKGVTELSS ASRFPVWASL 

       370        380        390        400        410 
VLYRKILDEI EANDYNNFTK RAYVSKSKKL IALPIAYAKS LVPPTKTASL QR 

« Hide

References

[1]"A tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway."
Bartley G.E., Viitanen P.V., Bacot K.O., Scolnik P.A.
J. Biol. Chem. 267:5036-5039(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]"Expression of the genes encoding the early carotenoid biosynthetic enzymes in Capsicum annuum."
Roemer S., Hugueney P., Bouvier F., Camara B., Kuntz M.
Biochem. Biophys. Res. Commun. 196:1414-1421(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Marmande.
[3]"Sequence of pTOM5, a ripening related cDNA from tomato."
Ray J., Bird C.R., Maunders M., Grierson D., Schuch W.
Nucleic Acids Res. 15:10587-10587(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Ailsa Craig.
[4]"Cloning and characterization of a gene involved in phytoene synthesis from tomato."
Ray J., Moureau P., Bird C., Bird A., Grierson D., Maunders M., Truesdale M., Bramley P., Schuch W.
Plant Mol. Biol. 19:401-404(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Ailsa Craig.
Tissue: Leaf.
[5]"Identification and genetic analysis of normal and mutant phytoene synthase genes of tomato by sequencing, complementation and co-suppression."
Fray R.G., Grierson D.
Plant Mol. Biol. 22:589-602(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTANT.
Strain: cv. Ailsa Craig.
Tissue: Pericarp.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84744 mRNA. Translation: AAA34153.1.
Y00521 mRNA. Translation: CAA68575.1.
X60441 Genomic DNA. Translation: CAA42969.1.
X67144 mRNA. Translation: CAA47625.1.
PIRA42102.
S22474.
RefSeqNP_001234812.1. NM_001247883.1.
UniGeneLes.13761.

3D structure databases

ProteinModelPortalP08196.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsSolyc03g031860.2.1; Solyc03g031860.2.1; Solyc03g031860.2.
GeneID543988.
KEGGsly:543988.

Phylogenomic databases

OMADDSIDVH.

Enzyme and pathway databases

SABIO-RKP08196.
UniPathwayUPA00799; UER00773.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSY1_SOLLC
AccessionPrimary (citable) accession number: P08196
Secondary accession number(s): Q40166
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways