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Protein

Phytoene synthase 1, chloroplastic

Gene

PSY1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reaction from prephytoene diphosphate to phytoene.

Catalytic activityi

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathwayi: phytoene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Phytoene synthase 1, chloroplastic (PSY1), Phytoene synthase 2, chloroplastic (PSY2)
This subpathway is part of the pathway phytoene biosynthesis, which is itself part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes all-trans-phytoene from geranylgeranyl diphosphate, the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processCarotenoid biosynthesis, Isoprene biosynthesis

Enzyme and pathway databases

BRENDAi2.5.1.32 3101
SABIO-RKiP08196
UniPathwayiUPA00799; UER00773

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene synthase 1, chloroplastic (EC:2.5.1.32)
Alternative name(s):
Fruit-ripening-specific protein pTOM5
Gene namesi
Name:PSY1
Synonyms:GTOM5, PTOM5, TOM5
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi388 – 412KKLIA…ASLQR → NMLKDFFSNFKGSKRGSNAT TTLVG: Lack of carotenoids in fruits. Add BLAST25

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 129ChloroplastSequence analysisAdd BLAST129
ChainiPRO_0000029856130 – 412Phytoene synthase 1, chloroplasticAdd BLAST283

Proteomic databases

PaxDbiP08196
PRIDEiP08196

Expressioni

Developmental stagei

In seedlings and in late stages of fruit ripening.

Inductioni

By fruit ripening.

Gene expression databases

ExpressionAtlasiP08196 baseline and differential

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi4081.Solyc03g031860.2.1

Structurei

3D structure databases

ProteinModelPortaliP08196
SMRiP08196
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1459 Eukaryota
COG1562 LUCA
InParanoidiP08196
KOiK02291
OMAiQLSIIHH
OrthoDBiEOG093609FK

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVALLWVVS PCDVSNGTSF MESVREGNRF FDSSRHRNLV SNERINRGGG
60 70 80 90 100
KQTNNGRKFS VRSAILATPS GERTMTSEQM VYDVVLRQAA LVKRQLRSTN
110 120 130 140 150
ELEVKPDIPI PGNLGLLSEA YDRCGEVCAE YAKTFNLGTM LMTPERRRAI
160 170 180 190 200
WAIYVWCRRT DELVDGPNAS YITPAALDRW ENRLEDVFNG RPFDMLDGAL
210 220 230 240 250
SDTVSNFPVD IQPFRDMIEG MRMDLRKSRY KNFDELYLYC YYVAGTVGLM
260 270 280 290 300
SVPIMGIAPE SKATTESVYN AALALGIANQ LTNILRDVGE DARRGRVYLP
310 320 330 340 350
QDELAQAGLS DEDIFAGRVT DKWRIFMKKQ IHRARKFFDE AEKGVTELSS
360 370 380 390 400
ASRFPVWASL VLYRKILDEI EANDYNNFTK RAYVSKSKKL IALPIAYAKS
410
LVPPTKTASL QR
Length:412
Mass (Da):46,615
Last modified:October 1, 1994 - v2
Checksum:iDBED05945D6F4C9A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti389 – 407KLIAL…PPTKT → QVDCITYCICKISCASYKN in CAA68575 (PubMed:3697097).CuratedAdd BLAST19
Sequence conflicti389 – 405KLIAL…LVPPT → QVDCITYCICKISCASY in CAA47625 (PubMed:1623189).CuratedAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84744 mRNA Translation: AAA34153.1
Y00521 mRNA Translation: CAA68575.1
X60441 Genomic DNA Translation: CAA42969.1
X67144 mRNA Translation: CAA47625.1
PIRiA42102
S22474
RefSeqiNP_001234812.1, NM_001247883.2
NP_001334763.1, NM_001347834.1
NP_001334765.1, NM_001347836.1
NP_001334767.1, NM_001347838.1
XP_010317654.1, XM_010319352.2
UniGeneiLes.13761
Les.19303

Genome annotation databases

EnsemblPlantsiSolyc03g031860.2.1; Solyc03g031860.2.1; Solyc03g031860.2
GeneIDi543988
GrameneiSolyc03g031860.2.1; Solyc03g031860.2.1; Solyc03g031860.2
KEGGisly:543988

Similar proteinsi

Entry informationi

Entry nameiPSY1_SOLLC
AccessioniPrimary (citable) accession number: P08196
Secondary accession number(s): Q40166
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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