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P08196

- PSY1_SOLLC

UniProt

P08196 - PSY1_SOLLC

Protein

Phytoene synthase 1, chloroplastic

Gene

PSY1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the reaction from prephytoene diphosphate to phytoene.

    Catalytic activityi

    2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carotenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carotenoid biosynthesis, Isoprene biosynthesis

    Enzyme and pathway databases

    SABIO-RKP08196.
    UniPathwayiUPA00799; UER00773.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phytoene synthase 1, chloroplastic (EC:2.5.1.32)
    Alternative name(s):
    Fruit-ripening-specific protein pTOM5
    Gene namesi
    Name:PSY1
    Synonyms:GTOM5, PTOM5, TOM5
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Chromosome 3

    Subcellular locationi

    GO - Cellular componenti

    1. plastoglobule Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi388 – 41225KKLIA…ASLQR → NMLKDFFSNFKGSKRGSNAT TTLVG: Lack of carotenoids in fruits. Add
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 129129ChloroplastSequence AnalysisAdd
    BLAST
    Chaini130 – 412283Phytoene synthase 1, chloroplasticPRO_0000029856Add
    BLAST

    Expressioni

    Developmental stagei

    In seedlings and in late stages of fruit ripening.

    Inductioni

    By fruit ripening.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP08196.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK02291.
    OMAiDDSIDVH.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08196-1 [UniParc]FASTAAdd to Basket

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    MSVALLWVVS PCDVSNGTSF MESVREGNRF FDSSRHRNLV SNERINRGGG    50
    KQTNNGRKFS VRSAILATPS GERTMTSEQM VYDVVLRQAA LVKRQLRSTN 100
    ELEVKPDIPI PGNLGLLSEA YDRCGEVCAE YAKTFNLGTM LMTPERRRAI 150
    WAIYVWCRRT DELVDGPNAS YITPAALDRW ENRLEDVFNG RPFDMLDGAL 200
    SDTVSNFPVD IQPFRDMIEG MRMDLRKSRY KNFDELYLYC YYVAGTVGLM 250
    SVPIMGIAPE SKATTESVYN AALALGIANQ LTNILRDVGE DARRGRVYLP 300
    QDELAQAGLS DEDIFAGRVT DKWRIFMKKQ IHRARKFFDE AEKGVTELSS 350
    ASRFPVWASL VLYRKILDEI EANDYNNFTK RAYVSKSKKL IALPIAYAKS 400
    LVPPTKTASL QR 412
    Length:412
    Mass (Da):46,615
    Last modified:October 1, 1994 - v2
    Checksum:iDBED05945D6F4C9A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti389 – 40719KLIAL…PPTKT → QVDCITYCICKISCASYKN in CAA68575. (PubMed:3697097)CuratedAdd
    BLAST
    Sequence conflicti389 – 40517KLIAL…LVPPT → QVDCITYCICKISCASY in CAA47625. (PubMed:1623189)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84744 mRNA. Translation: AAA34153.1.
    Y00521 mRNA. Translation: CAA68575.1.
    X60441 Genomic DNA. Translation: CAA42969.1.
    X67144 mRNA. Translation: CAA47625.1.
    PIRiA42102.
    S22474.
    RefSeqiNP_001234812.1. NM_001247883.1.
    UniGeneiLes.13761.

    Genome annotation databases

    EnsemblPlantsiSolyc03g031860.2.1; Solyc03g031860.2.1; Solyc03g031860.2.
    GeneIDi543988.
    KEGGisly:543988.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84744 mRNA. Translation: AAA34153.1 .
    Y00521 mRNA. Translation: CAA68575.1 .
    X60441 Genomic DNA. Translation: CAA42969.1 .
    X67144 mRNA. Translation: CAA47625.1 .
    PIRi A42102.
    S22474.
    RefSeqi NP_001234812.1. NM_001247883.1.
    UniGenei Les.13761.

    3D structure databases

    ProteinModelPortali P08196.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi Solyc03g031860.2.1 ; Solyc03g031860.2.1 ; Solyc03g031860.2 .
    GeneIDi 543988.
    KEGGi sly:543988.

    Phylogenomic databases

    KOi K02291.
    OMAi DDSIDVH.

    Enzyme and pathway databases

    UniPathwayi UPA00799 ; UER00773 .
    SABIO-RK P08196.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway."
      Bartley G.E., Viitanen P.V., Bacot K.O., Scolnik P.A.
      J. Biol. Chem. 267:5036-5039(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    2. "Expression of the genes encoding the early carotenoid biosynthetic enzymes in Capsicum annuum."
      Roemer S., Hugueney P., Bouvier F., Camara B., Kuntz M.
      Biochem. Biophys. Res. Commun. 196:1414-1421(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Marmande.
    3. "Sequence of pTOM5, a ripening related cDNA from tomato."
      Ray J., Bird C.R., Maunders M., Grierson D., Schuch W.
      Nucleic Acids Res. 15:10587-10587(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Ailsa Craig.
    4. "Cloning and characterization of a gene involved in phytoene synthesis from tomato."
      Ray J., Moureau P., Bird C., Bird A., Grierson D., Maunders M., Truesdale M., Bramley P., Schuch W.
      Plant Mol. Biol. 19:401-404(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Ailsa Craig.
      Tissue: Leaf.
    5. "Identification and genetic analysis of normal and mutant phytoene synthase genes of tomato by sequencing, complementation and co-suppression."
      Fray R.G., Grierson D.
      Plant Mol. Biol. 22:589-602(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTANT.
      Strain: cv. Ailsa Craig.
      Tissue: Pericarp.

    Entry informationi

    Entry nameiPSY1_SOLLC
    AccessioniPrimary (citable) accession number: P08196
    Secondary accession number(s): Q40166
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3