P08196 (PSY1_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytoene synthase 1, chloroplastic
EC=2.5.1.32

Alternative name(s):
Fruit-ripening-specific protein pTOM5

Gene names

Name:	PSY1
Synonyms:	GTOM5, PTOM5, TOM5

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene.
Catalytic activity	2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast.
Developmental stage	In seedlings and in late stages of fruit ripening.
Induction	By fruit ripening.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 129	129	Chloroplast Potential
	Chain	130 – 412	283	Phytoene synthase 1, chloroplastic		PRO_0000029856
Experimental info
	Mutagenesis	388 – 412	25	KKLIA…ASLQR → NMLKDFFSNFKGSKRGSNAT TTLVG: Lack of carotenoids in fruits.
	Sequence conflict	389 – 407	19	KLIAL…PPTKT → QVDCITYCICKISCASYKN in CAA68575. Ref.3
	Sequence conflict	389 – 405	17	KLIAL…LVPPT → QVDCITYCICKISCASY in CAA47625. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P08196 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: DBED05945D6F4C9A	FASTA	412	46,615
	10 20 30 40 50 60 MSVALLWVVS PCDVSNGTSF MESVREGNRF FDSSRHRNLV SNERINRGGG KQTNNGRKFS 70 80 90 100 110 120 VRSAILATPS GERTMTSEQM VYDVVLRQAA LVKRQLRSTN ELEVKPDIPI PGNLGLLSEA 130 140 150 160 170 180 YDRCGEVCAE YAKTFNLGTM LMTPERRRAI WAIYVWCRRT DELVDGPNAS YITPAALDRW 190 200 210 220 230 240 ENRLEDVFNG RPFDMLDGAL SDTVSNFPVD IQPFRDMIEG MRMDLRKSRY KNFDELYLYC 250 260 270 280 290 300 YYVAGTVGLM SVPIMGIAPE SKATTESVYN AALALGIANQ LTNILRDVGE DARRGRVYLP 310 320 330 340 350 360 QDELAQAGLS DEDIFAGRVT DKWRIFMKKQ IHRARKFFDE AEKGVTELSS ASRFPVWASL 370 380 390 400 410 VLYRKILDEI EANDYNNFTK RAYVSKSKKL IALPIAYAKS LVPPTKTASL QR

References

[1]	"A tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway." Bartley G.E., Viitanen P.V., Bacot K.O., Scolnik P.A. J. Biol. Chem. 267:5036-5039(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]	"Expression of the genes encoding the early carotenoid biosynthetic enzymes in Capsicum annuum." Roemer S., Hugueney P., Bouvier F., Camara B., Kuntz M. Biochem. Biophys. Res. Commun. 196:1414-1421(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Marmande.
[3]	"Sequence of pTOM5, a ripening related cDNA from tomato." Ray J., Bird C.R., Maunders M., Grierson D., Schuch W. Nucleic Acids Res. 15:10587-10587(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ailsa Craig.
[4]	"Cloning and characterization of a gene involved in phytoene synthesis from tomato." Ray J., Moureau P., Bird C., Bird A., Grierson D., Maunders M., Truesdale M., Bramley P., Schuch W. Plant Mol. Biol. 19:401-404(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Ailsa Craig. Tissue: Leaf.
[5]	"Identification and genetic analysis of normal and mutant phytoene synthase genes of tomato by sequencing, complementation and co-suppression." Fray R.G., Grierson D. Plant Mol. Biol. 22:589-602(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTANT. Strain: cv. Ailsa Craig. Tissue: Pericarp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84744 mRNA. Translation: AAA34153.1. Y00521 mRNA. Translation: CAA68575.1. X60441 Genomic DNA. Translation: CAA42969.1. X67144 mRNA. Translation: CAA47625.1.
PIR	A42102. S22474.
RefSeq	NP_001234812.1. NM_001247883.1.
UniGene	Les.13761.
3D structure databases
ProteinModelPortal	P08196.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	Solyc03g031860.2.1; Solyc03g031860.2.1; Solyc03g031860.2.
GeneID	543988.
Enzyme and pathway databases
SABIO-RK	P08196.
UniPathway	UPA00799; UER00773.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSY1_SOLLC

Accession

Primary (citable) accession number: P08196
Secondary accession number(s): Q40166

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program