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P08195 (4F2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 165. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4F2 cell-surface antigen heavy chain

Short name=4F2hc
Alternative name(s):
4F2 heavy chain antigen
Lymphocyte activation antigen 4F2 large subunit
Solute carrier family 3 member 2
CD_antigen=CD98
Gene names
Name:SLC3A2
Synonyms:MDU1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length630 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. Ref.6 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20 Ref.22 Ref.23 Ref.25 Ref.26 Ref.28 Ref.29

Subunit structure

Disulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). Colocalizes with cadherins By similarity. Interacts with FAM57A/CT120 and ICAM1. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1. Ref.6 Ref.12 Ref.13 Ref.14 Ref.16 Ref.17 Ref.18 Ref.19 Ref.21 Ref.23 Ref.25 Ref.26 Ref.29 Ref.44

Subcellular location

Apical cell membrane; Single-pass type II membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8. Localized to the placental apical membrane. Located selectively at cell-cell adhesion sites By similarity. Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells By similarity. Ref.16 Ref.17 Ref.20 Ref.23 Ref.26 Ref.32 Ref.33 Ref.44

Tissue specificity

Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1. Ref.4 Ref.5 Ref.6 Ref.17 Ref.20 Ref.25 Ref.26 Ref.28 Ref.32

Induction

Expression is induced in resting peripheral blood T-lymphocytes following PHA stimulation. Expression increases at the time of maximal DNA synthesis, in fibroblasts stimulated to divide. Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Ref.4 Ref.5 Ref.20

Post-translational modification

Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.

Miscellaneous

Arginine uptake is inhibited by increasing concentrations of leucine in the presence of Na+.

Sequence similarities

Belongs to the SLC3A transporter family.

Biophysicochemical properties

Kinetic parameters:

KM=295 µM for glutamine (in the presence of NaCl) Ref.15

KM=236 µM for leucine (in the presence of NaCl)

KM=120 µM for arginine (in the presence of NaCl)

KM=138 µM for arginine (in the absence of NaCl)

Mass spectrometry

Molecular mass is 57944.93 Da from positions 1 - 529. Determined by MALDI. Ref.24

Ontologies

Keywords
   Biological processAmino-acid transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   PTMAcetylation
Disulfide bond
Glycoprotein
Isopeptide bond
Phosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processamino acid transport

Traceable author statement PubMed 10673541. Source: UniProtKB

blood coagulation

Traceable author statement. Source: Reactome

calcium ion transport

Non-traceable author statement Ref.3. Source: UniProtKB

carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

cell growth

Non-traceable author statement Ref.1. Source: UniProtKB

ion transport

Traceable author statement. Source: Reactome

leucine import

Inferred from sequence or structural similarity. Source: UniProtKB

leukocyte migration

Traceable author statement. Source: Reactome

response to exogenous dsRNA

Inferred from mutant phenotype PubMed 21266579. Source: MGI

sodium ion transmembrane transport

Traceable author statement PubMed 10673541. Source: GOC

transmembrane transport

Traceable author statement. Source: Reactome

tryptophan transport

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell junction

Inferred from direct assay. Source: HPA

cytoplasm

Inferred from direct assay. Source: HPA

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867PubMed 19199708PubMed 20458337. Source: UniProt

integral component of membrane

Non-traceable author statement Ref.1. Source: UniProtKB

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay. Source: HPA

plasma membrane

Inferred from direct assay. Source: HPA

   Molecular_functioncalcium:sodium antiporter activity

Traceable author statement PubMed 10673541. Source: UniProtKB

catalytic activity

Inferred from electronic annotation. Source: InterPro

cation binding

Inferred from electronic annotation. Source: InterPro

double-stranded RNA binding

Inferred from direct assay PubMed 21266579. Source: MGI

neutral amino acid transmembrane transporter activity

Inferred from sequence or structural similarity. Source: UniProtKB

poly(A) RNA binding

Inferred from direct assay PubMed 22658674. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P08195-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P08195-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-101: Missing.
Isoform 3 (identifier: P08195-3)

The sequence of this isoform differs from the canonical sequence as follows:
     38-99: Missing.
Isoform 4 (identifier: P08195-4)

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: V → VTETGFHHVSQADIEFLTSIDPTASASGSAGI

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6306304F2 cell-surface antigen heavy chain
PRO_0000064383

Regions

Topological domain102 – 18483Cytoplasmic Potential
Transmembrane185 – 20521Helical; Signal-anchor for type II membrane protein; Potential
Topological domain206 – 630425Extracellular Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.9 Ref.43
Modified residue1031Phosphoserine Ref.41
Modified residue1341Phosphoserine Ref.41
Modified residue1651Phosphoserine Ref.39
Modified residue4061Phosphoserine Probable
Modified residue4081Phosphoserine Probable
Modified residue4101Phosphoserine Probable
Modified residue5271Phosphoserine Probable
Modified residue5311Phosphoserine Probable
Glycosylation3651N-linked (GlcNAc...) Ref.27 Ref.36 Ref.38
Glycosylation3811N-linked (GlcNAc...) Ref.27 Ref.30 Ref.36 Ref.38
Glycosylation4241N-linked (GlcNAc...) (complex) Ref.27 Ref.30 Ref.37 Ref.38
Glycosylation5061N-linked (GlcNAc...) Ref.36 Ref.38
Disulfide bond210Interchain (with light chain) Ref.44
Cross-link147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Natural variations

Alternative sequence1 – 101101Missing in isoform 2.
VSP_037907
Alternative sequence38 – 9962Missing in isoform 3.
VSP_037908
Alternative sequence981V → VTETGFHHVSQADIEFLTSI DPTASASGSAGI in isoform 4.
VSP_037909

Experimental info

Mutagenesis2101C → S: Abolishes dimerization, leucine uptake and interaction with beta-1 integrins. Ref.12 Ref.18 Ref.44
Mutagenesis4311C → S: No effect on dimerization, leucine uptake or interaction with beta-1 integrins. Ref.12 Ref.18
Sequence conflict1371G → E in AAA35536. Ref.4
Sequence conflict1581A → P in AAA51540. Ref.3
Sequence conflict2231A → P in AAA35536. Ref.4
Sequence conflict3151E → D in AAA35536. Ref.4
Sequence conflict3201S → F in AAA35489. Ref.5
Sequence conflict3721E → G in AAA35536. Ref.4
Sequence conflict412 – 4132GE → PQ in AAA35536. Ref.4
Sequence conflict4651V → L in AAA35536. Ref.4
Sequence conflict4811G → P in AAA35536. Ref.4
Sequence conflict5491G → E in AAA35489. Ref.5
Sequence conflict6091L → P in AAA35536. Ref.4
Sequence conflict6121E → G in AAA35536. Ref.4

Secondary structure

.............................................................................................. 630
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 1, 2009. Version 3.
Checksum: AE427F8204CC10B0

FASTA63067,994
        10         20         30         40         50         60 
MELQPPEASI AVVSIPRQLP GSHSEAGVQG LSAGDDSELG SHCVAQTGLE LLASGDPLPS 

        70         80         90        100        110        120 
ASQNAEMIET GSDCVTQAGL QLLASSDPPA LASKNAEVTG TMSQDTEVDM KEVELNELEP 

       130        140        150        160        170        180 
EKQPMNAASG AAMSLAGAEK NGLVKIKVAE DEAEAAAAAK FTGLSKEELL KVAGSPGWVR 

       190        200        210        220        230        240 
TRWALLLLFW LGWLGMLAGA VVIIVRAPRC RELPAQKWWH TGALYRIGDL QAFQGHGAGN 

       250        260        270        280        290        300 
LAGLKGRLDY LSSLKVKGLV LGPIHKNQKD DVAQTDLLQI DPNFGSKEDF DSLLQSAKKK 

       310        320        330        340        350        360 
SIRVILDLTP NYRGENSWFS TQVDTVATKV KDALEFWLQA GVDGFQVRDI ENLKDASSFL 

       370        380        390        400        410        420 
AEWQNITKGF SEDRLLIAGT NSSDLQQILS LLESNKDLLL TSSYLSDSGS TGEHTKSLVT 

       430        440        450        460        470        480 
QYLNATGNRW CSWSLSQARL LTSFLPAQLL RLYQLMLFTL PGTPVFSYGD EIGLDAAALP 

       490        500        510        520        530        540 
GQPMEAPVML WDESSFPDIP GAVSANMTVK GQSEDPGSLL SLFRRLSDQR SKERSLLHGD 

       550        560        570        580        590        600 
FHAFSAGPGL FSYIRHWDQN ERFLVVLNFG DVGLSAGLQA SDLPASASLP AKADLLLSTQ 

       610        620        630 
PGREEGSPLE LERLKLEPHE GLLLRFPYAA 

« Hide

Isoform 2 [UniParc].

Checksum: 82F26856737E8F31
Show »

FASTA52957,945
Isoform 3 [UniParc].

Checksum: F2B9400F95F2E1BD
Show »

FASTA56861,816
Isoform 4 [UniParc].

Checksum: 06C2CDFD6B1D93F5
Show »

FASTA66171,123

References

« Hide 'large scale' references
[1]"Molecular cloning of complementary DNAs encoding the heavy chain of the human 4F2 cell-surface antigen: a type II membrane glycoprotein involved in normal and neoplastic cell growth."
Quackenbush E., Clabby M., Gottesdiener K.M., Barbosa J., Jones N.H., Strominger J.L., Speck S., Leiden J.M.
Proc. Natl. Acad. Sci. U.S.A. 84:6526-6530(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]Erratum
Quackenbush E., Clabby M., Gottesdiener K.M., Barbosa J., Jones N.H., Strominger J.L., Speck S., Leiden J.M.
Proc. Natl. Acad. Sci. U.S.A. 84:8618-8618(1987)
Cited for: SEQUENCE REVISION.
[3]"Primary structure of the human 4F2 antigen heavy chain predicts a transmembrane protein with a cytoplasmic NH2 terminus."
Teixeira S., di Grandi S., Kuehn L.C.
J. Biol. Chem. 262:9574-9580(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]"Cloning, sequence analysis, and expression of the large subunit of the human lymphocyte activation antigen 4F2."
Lumadue J.A., Glick A.B., Ruddle F.H.
Proc. Natl. Acad. Sci. U.S.A. 84:9204-9208(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, INDUCTION.
Tissue: Fibroblast.
[5]"Isolation and structural characterization of the human 4F2 heavy-chain gene, an inducible gene involved in T-lymphocyte activation."
Gottesdiener K.M., Karpinski B.A., Lindsten T., Strominger J.L., Jones N.H., Thompson C.B., Leiden J.M.
Mol. Cell. Biol. 8:3809-3819(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2), TISSUE SPECIFICITY, INDUCTION.
[6]"Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines."
Yanagida O., Kanai Y., Chairoungdua A., Kim D.K., Segawa H., Nii T., Cha S.H., Matsuo H., Fukushima J., Fukasawa Y., Tani Y., Taketani Y., Uchino H., Kim J.Y., Inatomi J., Okayasu I., Miyamoto K., Takeda E., Goya T., Endou H.
Biochim. Biophys. Acta 1514:291-302(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
Tissue: Placenta.
[7]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 4).
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Lung and Placenta.
[9]Bienvenut W.V., Lao L., Ryan K.L.
Submitted (OCT-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1-17; 146-171; 227-245; 304-313; 440-451; 511-525 AND 593-630, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[10]Strausberg R.L.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 83-209 (ISOFORM 4).
Tissue: Lung carcinoma.
[11]Bienvenut W.V.
Submitted (MAR-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 112-122; 148-160; 227-245; 248-255; 288-298; 304-313; 440-451; 511-524 AND 593-625, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: B-cell lymphoma.
[12]"Identification and characterization of a membrane protein (y+L amino acid transporter-1) that associates with 4F2hc to encode the amino acid transport activity y+L. A candidate gene for lysinuric protein intolerance."
Torrents D., Estevez R., Pineda M., Fernandez E., Lloberas J., Shi Y.-B., Zorzano A., Palacin M.
J. Biol. Chem. 273:32437-32445(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INHIBITION, MUTAGENESIS OF CYS-210 AND CYS-431.
[13]"Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family."
Mastroberardino L., Spindler B., Pfeiffer R., Skelly P.J., Loffing J., Shoemaker C.B., Verrey F.
Nature 395:288-291(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[14]"Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid transporter family."
Pfeiffer R., Rossier G., Spindler B., Meier C., Kuehn L.C., Verrey F.
EMBO J. 18:49-57(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[15]"The heterodimeric amino acid transporter 4F2hc/y+LAT2 mediates arginine efflux in exchange with glutamine."
Broeer A., Wagner C.A., Lang F., Broeer S.
Biochem. J. 349:787-795(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[16]"Association of 4F2hc with light chains LAT1, LAT2 or y+LAT2 requires different domains."
Broeer A., Friedrich B., Wagner C.A., Fillon S., Ganapathy V., Lang F., Broeer S.
Biochem. J. 355:725-731(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[17]"Role of the System L permease LAT1 in amino acid and iodothyronine transport in placenta."
Ritchie J.W.A., Taylor P.M.
Biochem. J. 356:719-725(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[18]"Beta1 integrins show specific association with CD98 protein in low density membranes."
Kolesnikova T.V., Mannion B.A., Berditchevski F., Hemler M.E.
BMC Biochem. 2:10-10(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH BETA-1 INTEGRINS, MUTAGENESIS OF CYS-210 AND CYS-431.
[19]"Thyroid hormone transport by the heterodimeric human system L amino acid transporter."
Friesema E.C.H., Docter R., Moerings E.P.C.M., Verrey F., Krenning E.P., Hennemann G., Visser T.J.
Endocrinology 142:4339-4348(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[20]"Expression and regulation of 4F2hc and hLAT1 in human trophoblasts."
Okamoto Y., Sakata M., Ogura K., Yamamoto T., Yamaguchi M., Tasaka K., Kurachi H., Tsurudome M., Murata Y.
Am. J. Physiol. 282:C196-C204(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[21]"Molecular cloning and characterization of CT120, a novel membrane-associated gene involved in amino acid transport and glutathione metabolism."
He X.H., Di Y., Li J., Xie Y., Tang Y., Zhang F., Wei L., Zhang Y., Qin W.X., Huo K., Li Y., Wan D.F., Gu J.R.
Biochem. Biophys. Res. Commun. 297:528-536(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FAM57A/CT120.
[22]"Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2."
Simmons-Willis T.A., Koh A.S., Clarkson T.W., Ballatori N.
Biochem. J. 367:239-246(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[23]"Characterization of the system L amino acid transporter in T24 human bladder carcinoma cells."
Kim D.K., Kanai Y., Choi H.W., Tangtrongsup S., Chairoungdua A., Babu E., Tachampa K., Anzai N., Iribe Y., Endou H.
Biochim. Biophys. Acta 1565:112-121(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[24]"Cluster analysis of an extensive human breast cancer cell line protein expression map database."
Harris R.A., Yang A., Stein R.C., Lucy K., Brusten L., Herath A., Parekh R., Waterfield M.D., O'Hare M.J., Neville M.A., Page M.J., Zvelebil M.J.
Proteomics 2:212-223(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
Tissue: Mammary cancer.
[25]"Nitric oxide synthesis requires activity of the cationic and neutral amino acid transport system y+L in human umbilical vein endothelium."
Arancibia-Garavilla Y., Toledo F., Casanello P., Sobrevia L.
Exp. Physiol. 88:699-710(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
[26]"CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT-2 in polarized intestinal epithelia."
Liu X., Charrier L., Gewirtz A., Sitaraman S., Merlin D.
J. Biol. Chem. 278:23672-23677(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH ICAM1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[27]"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
Zhang H., Li X.-J., Martin D.B., Aebersold R.
Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-365; ASN-381 AND ASN-424.
[28]"L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier."
Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K.
Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[29]"Identification of stereoselective transporters for S-nitroso-L-cysteine: role of LAT1 and LAT2 in biological activity of S-nitrosothiols."
Li S., Whorton A.R.
J. Biol. Chem. 280:20102-20110(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[30]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-381 AND ASN-424.
Tissue: Plasma.
[31]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[32]"L-type amino acid transporter 1 as a potential molecular target in human astrocytic tumors."
Nawashiro H., Otani N., Shinomiya N., Fukui S., Ooigawa H., Shima K., Matsuo H., Kanai Y., Endou H.
Int. J. Cancer 119:484-492(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[33]"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F.
J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Melanoma.
[34]"Ecto-phosphorylation of CD98 regulates cell-cell interactions."
Nguyen H.T.T., Dalmasso G., Yan Y., Obertone T.S., Sitaraman S.V., Merlin D.
PLoS ONE 3:E3895-E3895(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-406; SER-408; SER-410; SER-527 AND SER-531.
[35]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[36]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-365; ASN-381 AND ASN-506.
Tissue: Liver.
[37]"A strategy for precise and large scale identification of core fucosylated glycoproteins."
Jia W., Lu Z., Fu Y., Wang H.P., Wang L.H., Chi H., Yuan Z.F., Zheng Z.B., Song L.N., Han H.H., Liang Y.M., Wang J.L., Cai Y., Zhang Y.K., Deng Y.L., Ying W.T., He S.M., Qian X.H.
Mol. Cell. Proteomics 8:913-923(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-424.
[38]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-365; ASN-381; ASN-424 AND ASN-506.
Tissue: Leukemic T-cell.
[39]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[40]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[41]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-103 AND SER-134, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[42]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[43]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[44]"The structure of human 4F2hc ectodomain provides a model for homodimerization and electrostatic interaction with plasma membrane."
Fort J., de la Ballina L.R., Burghardt H.E., Ferrer-Costa C., Turnay J., Ferrer-Orta C., Uson I., Zorzano A., Fernandez-Recio J., Orozco M., Lizarbe M.A., Fita I., Palacin M.
J. Biol. Chem. 282:31444-31452(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 212-630, SUBUNIT, MUTAGENESIS OF CYS-210, SUBCELLULAR LOCATION, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02939 mRNA. Translation: AAA52497.1.
J02769 mRNA. Translation: AAA51540.1.
J03569 mRNA. Translation: AAA35536.1.
M21904 expand/collapse EMBL AC list , M21898, M21899, M21900, M21901, M21902, M21903 Genomic DNA. Translation: AAA35489.1.
AB018010 mRNA. Translation: BAA84649.1.
AP001160 Genomic DNA. No translation available.
BC001061 mRNA. Translation: AAH01061.2.
BC003000 mRNA. Translation: AAH03000.2.
BE794697 mRNA. No translation available.
PIRSAHU4F. A28455.
RefSeqNP_001012680.1. NM_001012662.2.
NP_001012682.1. NM_001012664.2.
NP_001013269.1. NM_001013251.2.
NP_002385.3. NM_002394.5.
UniGeneHs.502769.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DH2X-ray2.10A212-630[»]
2DH3X-ray2.80A/B212-630[»]
ProteinModelPortalP08195.
SMRP08195. Positions 212-630.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112411. 27 interactions.
IntActP08195. 11 interactions.
MINTMINT-4999018.
STRING9606.ENSP00000367124.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.
TCDB8.A.9.2.2. the rbat transport accessory protein (rbat) family.

PTM databases

PhosphoSiteP08195.

Polymorphism databases

DMDM257051063.

Proteomic databases

PaxDbP08195.
PRIDEP08195.

Protocols and materials databases

DNASU6520.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000338663; ENSP00000340815; ENSG00000168003. [P08195-2]
ENST00000377889; ENSP00000367121; ENSG00000168003. [P08195-3]
ENST00000377890; ENSP00000367122; ENSG00000168003. [P08195-1]
ENST00000377892; ENSP00000367124; ENSG00000168003. [P08195-4]
GeneID6520.
KEGGhsa:6520.
UCSCuc001nwd.3. human. [P08195-1]
uc001nwf.3. human. [P08195-3]

Organism-specific databases

CTD6520.
GeneCardsGC11P062623.
HGNCHGNC:11026. SLC3A2.
HPACAB010455.
HPA017980.
MIM158070. gene.
neXtProtNX_P08195.
PharmGKBPA35894.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0366.
HOGENOMHOG000233529.
HOVERGENHBG000023.
InParanoidP08195.
KOK06519.
OMANMTVKGQ.
OrthoDBEOG7ZSHSV.
PhylomeDBP08195.

Enzyme and pathway databases

BioCycMetaCyc:ENSG00000168003-MONOMER.
ReactomeREACT_15518. Transmembrane transport of small molecules.
REACT_19419. Amino acid and oligopeptide SLC transporters.
REACT_604. Hemostasis.
SABIO-RKP08195.

Gene expression databases

ArrayExpressP08195.
BgeeP08195.
CleanExHS_SLC3A2.
GenevestigatorP08195.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 2 hits.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSLC3A2. human.
EvolutionaryTraceP08195.
GeneWikiSLC3A2.
GenomeRNAi6520.
NextBio25353.
PROP08195.
SOURCESearch...

Entry information

Entry name4F2_HUMAN
AccessionPrimary (citable) accession number: P08195
Secondary accession number(s): Q13543
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: September 1, 2009
Last modified: April 16, 2014
This is version 165 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries