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Protein

Glycerol-3-phosphate transporter

Gene

glpT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for glycerol-3-phosphate uptake.

GO - Molecular functioni

  • glycerol-3-phosphate transmembrane transporter activity Source: InterPro
  • glycerol-phosphate:inorganic phosphate antiporter activity Source: EcoCyc
  • organophosphate:inorganic phosphate antiporter activity Source: EcoCyc

GO - Biological processi

  • glycerol-3-phosphate transport Source: EcoCyc
  • glycerol metabolic process Source: UniProtKB-KW
  • glycerol transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Glycerol metabolism, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GLPT-MONOMER.
ECOL316407:JW2234-MONOMER.
MetaCyc:GLPT-MONOMER.

Protein family/group databases

TCDBi2.A.1.4.3. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate transporter
Short name:
G-3-P transporter
Alternative name(s):
G-3-P permease
Gene namesi
Name:glpT
Ordered Locus Names:b2240, JW2234
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10401. glpT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3636CytoplasmicAdd
BLAST
Transmembranei37 – 5721Helical; Name=1Add
BLAST
Topological domaini58 – 636Periplasmic
Transmembranei64 – 8421Helical; Name=2Add
BLAST
Topological domaini85 – 939Cytoplasmic
Transmembranei94 – 11219Helical; Name=3Add
BLAST
Topological domaini113 – 1208Periplasmic
Transmembranei121 – 14121Helical; Name=4Add
BLAST
Topological domaini142 – 16019CytoplasmicAdd
BLAST
Transmembranei161 – 18020Helical; Name=5Add
BLAST
Topological domaini181 – 1899Periplasmic
Transmembranei190 – 20718Helical; Name=6Add
BLAST
Topological domaini208 – 26154CytoplasmicAdd
BLAST
Transmembranei262 – 28221Helical; Name=7Add
BLAST
Topological domaini283 – 2875Periplasmic
Transmembranei288 – 30821Helical; Name=8Add
BLAST
Topological domaini309 – 32113CytoplasmicAdd
BLAST
Transmembranei322 – 34120Helical; Name=9Add
BLAST
Topological domaini342 – 3465Periplasmic
Transmembranei347 – 36822Helical; Name=10Add
BLAST
Topological domaini369 – 38719CytoplasmicAdd
BLAST
Transmembranei388 – 40922Helical; Name=11Add
BLAST
Topological domaini410 – 4145Periplasmic
Transmembranei415 – 43521Helical; Name=12Add
BLAST
Topological domaini436 – 45217CytoplasmicAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Glycerol-3-phosphate transporterPRO_0000199878Add
BLAST

Proteomic databases

PaxDbiP08194.
PRIDEiP08194.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4261310. 156 interactions.
IntActiP08194. 1 interaction.
STRINGi511145.b2240.

Structurei

Secondary structure

1
452
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 4526Combined sources
Helixi48 – 547Combined sources
Helixi65 – 8925Combined sources
Helixi92 – 11221Combined sources
Helixi114 – 1174Combined sources
Beta strandi118 – 1214Combined sources
Helixi122 – 13615Combined sources
Helixi138 – 1469Combined sources
Turni150 – 1523Combined sources
Helixi153 – 16917Combined sources
Helixi172 – 18211Combined sources
Helixi191 – 20717Combined sources
Turni213 – 2153Combined sources
Turni221 – 2233Combined sources
Helixi243 – 2486Combined sources
Helixi253 – 27927Combined sources
Helixi288 – 31528Combined sources
Helixi321 – 33818Combined sources
Helixi347 – 36115Combined sources
Helixi363 – 37412Combined sources
Helixi380 – 40930Combined sources
Helixi414 – 44330Combined sources
Helixi444 – 4485Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PW4X-ray3.30A3-448[»]
ProteinModelPortaliP08194.
SMRiP08194. Positions 5-448.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08194.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CXY. Bacteria.
COG2271. LUCA.
HOGENOMiHOG000274729.
InParanoidiP08194.
KOiK02445.
OMAiAPWVSAS.
OrthoDBiEOG6M9DW7.
PhylomeDBiP08194.

Family and domain databases

InterProiIPR005267. G3P_transporter.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR021159. Sugar-P_transporter_CS.
IPR000849. Sugar_P_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002808. Hexose_phosphate_transp. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00712. glpT. 1 hit.
PROSITEiPS00942. GLPT. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSIFKPAPH KARLPAAEID PTYRRLRWQI FLGIFFGYAA YYLVRKNFAL
60 70 80 90 100
AMPYLVEQGF SRGDLGFALS GISIAYGFSK FIMGSVSDRS NPRVFLPAGL
110 120 130 140 150
ILAAAVMLFM GFVPWATSSI AVMFVLLFLC GWFQGMGWPP CGRTMVHWWS
160 170 180 190 200
QKERGGIVSV WNCAHNVGGG IPPLLFLLGM AWFNDWHAAL YMPAFCAILV
210 220 230 240 250
ALFAFAMMRD TPQSCGLPPI EEYKNDYPDD YNEKAEQELT AKQIFMQYVL
260 270 280 290 300
PNKLLWYIAI ANVFVYLLRY GILDWSPTYL KEVKHFALDK SSWAYFLYEY
310 320 330 340 350
AGIPGTLLCG WMSDKVFRGN RGATGVFFMT LVTIATIVYW MNPAGNPTVD
360 370 380 390 400
MICMIVIGFL IYGPVMLIGL HALELAPKKA AGTAAGFTGL FGYLGGSVAA
410 420 430 440 450
SAIVGYTVDF FGWDGGFMVM IGGSILAVIL LIVVMIGEKR RHEQLLQERN

GG
Length:452
Mass (Da):50,310
Last modified:August 1, 1988 - v1
Checksum:i0DE70D08D40AD445
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00536 Genomic DNA. Translation: CAA68598.1.
U00096 Genomic DNA. Translation: AAC75300.1.
AP009048 Genomic DNA. Translation: BAA16059.1.
PIRiS00868. JNECGT.
RefSeqiNP_416743.1. NC_000913.3.
WP_000948731.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75300; AAC75300; b2240.
BAA16059; BAA16059; BAA16059.
GeneIDi946704.
KEGGiecj:JW2234.
eco:b2240.
PATRICi32119839. VBIEscCol129921_2329.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00536 Genomic DNA. Translation: CAA68598.1.
U00096 Genomic DNA. Translation: AAC75300.1.
AP009048 Genomic DNA. Translation: BAA16059.1.
PIRiS00868. JNECGT.
RefSeqiNP_416743.1. NC_000913.3.
WP_000948731.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PW4X-ray3.30A3-448[»]
ProteinModelPortaliP08194.
SMRiP08194. Positions 5-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261310. 156 interactions.
IntActiP08194. 1 interaction.
STRINGi511145.b2240.

Protein family/group databases

TCDBi2.A.1.4.3. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP08194.
PRIDEiP08194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75300; AAC75300; b2240.
BAA16059; BAA16059; BAA16059.
GeneIDi946704.
KEGGiecj:JW2234.
eco:b2240.
PATRICi32119839. VBIEscCol129921_2329.

Organism-specific databases

EchoBASEiEB0396.
EcoGeneiEG10401. glpT.

Phylogenomic databases

eggNOGiENOG4105CXY. Bacteria.
COG2271. LUCA.
HOGENOMiHOG000274729.
InParanoidiP08194.
KOiK02445.
OMAiAPWVSAS.
OrthoDBiEOG6M9DW7.
PhylomeDBiP08194.

Enzyme and pathway databases

BioCyciEcoCyc:GLPT-MONOMER.
ECOL316407:JW2234-MONOMER.
MetaCyc:GLPT-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP08194.
PROiP08194.

Family and domain databases

InterProiIPR005267. G3P_transporter.
IPR011701. MFS.
IPR020846. MFS_dom.
IPR021159. Sugar-P_transporter_CS.
IPR000849. Sugar_P_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002808. Hexose_phosphate_transp. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00712. glpT. 1 hit.
PROSITEiPS00942. GLPT. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phosphate transport protein with components of the hexose-6-phosphate transport system."
    Eiglmeier K., Boos W., Cole S.
    Mol. Microbiol. 1:251-258(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The transmembrane topology of the sn-glycerol-3-phosphate permease of Escherichia coli analysed by phoA and lacZ protein fusions."
    Goett P., Boos W.
    Mol. Microbiol. 2:655-663(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli."
    Huang Y., Lemieux M.J., Song J., Auer M., Wang D.-N.
    Science 301:616-620(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 5-451.

Entry informationi

Entry nameiGLPT_ECOLI
AccessioniPrimary (citable) accession number: P08194
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: July 6, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.