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P08179

- PUR3_ECOLI

UniProt

P08179 - PUR3_ECOLI

Protein

Phosphoribosylglycinamide formyltransferase

Gene

purN

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 64110-formyltetrahydrofolate
    Binding sitei106 – 106110-formyltetrahydrofolate
    Active sitei108 – 1081Proton donor2 Publications
    Sitei144 – 1441Raises pKa of active site His

    GO - Molecular functioni

    1. methyltransferase activity Source: InterPro
    2. phosphoribosylglycinamide formyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. cellular response to DNA damage stimulus Source: EcoliWiki

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:GART-MONOMER.
    ECOL316407:JW2485-MONOMER.
    MetaCyc:GART-MONOMER.
    RETL1328306-WGS:GSTH-1500-MONOMER.
    UniPathwayiUPA00074; UER00126.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name:
    GART
    Gene namesi
    Name:purN
    Ordered Locus Names:b2500, JW2485
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10799. purN.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi70 – 701E → A: Loss of homodimerization. No effect on activity.
    Mutagenesisi106 – 1061N → A, D, G or S: Reduces activity about 2000-fold.
    Mutagenesisi106 – 1061N → E, H, I, K, L or Y: Loss of activity.
    Mutagenesisi108 – 1081H → A, G, M, N, Q or R: Loss of activity.
    Mutagenesisi108 – 1081H → E, S or T: Reduces activity about 1000-fold.
    Mutagenesisi119 – 1191H → A: No effect.
    Mutagenesisi121 – 1211H → Q: Increases Km for 5'-phosphoribosylglycinamide 4-fold.
    Mutagenesisi135 – 1351S → A or L: Reduces activity about 1000-fold.
    Mutagenesisi137 – 1371H → F or Q: No effect.
    Mutagenesisi144 – 1441D → A, E, S or Y: Reduces activity about 1000-fold.
    Mutagenesisi144 – 1441D → C, F, H, K, L, N, P, Q, R, T or V: Loss of activity.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 212212Phosphoribosylglycinamide formyltransferasePRO_0000074943Add
    BLAST

    Proteomic databases

    PaxDbiP08179.
    PRIDEiP08179.

    Expressioni

    Gene expression databases

    GenevestigatoriP08179.

    Interactioni

    Subunit structurei

    Monomer. Homodimer below pH 6.8.4 Publications

    Protein-protein interaction databases

    IntActiP08179. 1 interaction.
    STRINGi511145.b2500.

    Structurei

    Secondary structure

    1
    212
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 98
    Helixi12 – 2211
    Beta strandi25 – 3612
    Helixi41 – 488
    Beta strandi52 – 554
    Helixi58 – 603
    Beta strandi61 – 633
    Helixi64 – 7512
    Helixi76 – 783
    Beta strandi81 – 877
    Helixi94 – 996
    Turni100 – 1023
    Beta strandi103 – 1108
    Beta strandi112 – 1143
    Helixi120 – 1267
    Beta strandi130 – 1389
    Beta strandi141 – 1466
    Beta strandi148 – 1558
    Helixi162 – 18524
    Beta strandi189 – 1924
    Beta strandi195 – 1984

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1C2TX-ray2.10A/B1-212[»]
    1C3EX-ray2.10A/B1-209[»]
    1CDDX-ray2.80A/B1-212[»]
    1CDEX-ray2.50A/B/C/D1-212[»]
    1GARX-ray1.96A/B1-212[»]
    1GRCX-ray3.00A/B1-212[»]
    1JKXX-ray1.60A/B/C/D1-212[»]
    2GARX-ray1.80A1-212[»]
    3GARX-ray1.90A1-212[»]
    ProteinModelPortaliP08179.
    SMRiP08179. Positions 1-209.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08179.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni11 – 1335'-phosphoribosylglycinamide binding
    Regioni89 – 92410-formyltetrahydrofolate binding
    Regioni140 – 144510-formyltetrahydrofolate binding
    Regioni170 – 17345'-phosphoribosylglycinamide binding

    Sequence similaritiesi

    Belongs to the GART family.Curated

    Phylogenomic databases

    eggNOGiCOG0299.
    HOGENOMiHOG000033575.
    KOiK11175.
    OMAiSPAFVAH.
    OrthoDBiEOG615VP4.
    PhylomeDBiP08179.

    Family and domain databases

    Gene3Di3.40.50.170. 1 hit.
    InterProiIPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR004607. PurN_trans.
    [Graphical view]
    PfamiPF00551. Formyl_trans_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53328. SSF53328. 1 hit.
    TIGRFAMsiTIGR00639. PurN. 1 hit.
    PROSITEiPS00373. GART. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08179-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIVVLISGN GSNLQAIIDA CKTNKIKGTV RAVFSNKADA FGLERARQAG    50
    IATHTLIASA FDSREAYDRE LIHEIDMYAP DVVVLAGFMR ILSPAFVSHY 100
    AGRLLNIHPS LLPKYPGLHT HRQALENGDE EHGTSVHFVT DELDGGPVIL 150
    QAKVPVFAGD SEDDITARVQ TQEHAIYPLV ISWFADGRLK MHENAAWLDG 200
    QRLPPQGYAA DE 212
    Length:212
    Mass (Da):23,238
    Last modified:August 1, 1988 - v1
    Checksum:iD38F326BCBA103FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13747 Genomic DNA. Translation: AAA83899.1.
    U00096 Genomic DNA. Translation: AAC75553.1.
    AP009048 Genomic DNA. Translation: BAA16388.1.
    AF293167 Genomic DNA. Translation: AAG14584.1.
    PIRiA28486. XYECGF.
    RefSeqiNP_416995.1. NC_000913.3.
    YP_490728.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75553; AAC75553; b2500.
    BAA16388; BAA16388; BAA16388.
    GeneIDi12932446.
    946973.
    KEGGiecj:Y75_p2453.
    eco:b2500.
    PATRICi32120389. VBIEscCol129921_2597.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13747 Genomic DNA. Translation: AAA83899.1 .
    U00096 Genomic DNA. Translation: AAC75553.1 .
    AP009048 Genomic DNA. Translation: BAA16388.1 .
    AF293167 Genomic DNA. Translation: AAG14584.1 .
    PIRi A28486. XYECGF.
    RefSeqi NP_416995.1. NC_000913.3.
    YP_490728.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1C2T X-ray 2.10 A/B 1-212 [» ]
    1C3E X-ray 2.10 A/B 1-209 [» ]
    1CDD X-ray 2.80 A/B 1-212 [» ]
    1CDE X-ray 2.50 A/B/C/D 1-212 [» ]
    1GAR X-ray 1.96 A/B 1-212 [» ]
    1GRC X-ray 3.00 A/B 1-212 [» ]
    1JKX X-ray 1.60 A/B/C/D 1-212 [» ]
    2GAR X-ray 1.80 A 1-212 [» ]
    3GAR X-ray 1.90 A 1-212 [» ]
    ProteinModelPortali P08179.
    SMRi P08179. Positions 1-209.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08179. 1 interaction.
    STRINGi 511145.b2500.

    Chemistry

    BindingDBi P08179.

    Proteomic databases

    PaxDbi P08179.
    PRIDEi P08179.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75553 ; AAC75553 ; b2500 .
    BAA16388 ; BAA16388 ; BAA16388 .
    GeneIDi 12932446.
    946973.
    KEGGi ecj:Y75_p2453.
    eco:b2500.
    PATRICi 32120389. VBIEscCol129921_2597.

    Organism-specific databases

    EchoBASEi EB0792.
    EcoGenei EG10799. purN.

    Phylogenomic databases

    eggNOGi COG0299.
    HOGENOMi HOG000033575.
    KOi K11175.
    OMAi SPAFVAH.
    OrthoDBi EOG615VP4.
    PhylomeDBi P08179.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00126 .
    BioCyci EcoCyc:GART-MONOMER.
    ECOL316407:JW2485-MONOMER.
    MetaCyc:GART-MONOMER.
    RETL1328306-WGS:GSTH-1500-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P08179.
    PROi P08179.

    Gene expression databases

    Genevestigatori P08179.

    Family and domain databases

    Gene3Di 3.40.50.170. 1 hit.
    InterProi IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR004607. PurN_trans.
    [Graphical view ]
    Pfami PF00551. Formyl_trans_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53328. SSF53328. 1 hit.
    TIGRFAMsi TIGR00639. PurN. 1 hit.
    PROSITEi PS00373. GART. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12."
      Smith J.M., Daum H.A. III
      J. Biol. Chem. 262:10565-10569(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics."
      Pupo G.M., Lan R., Reeves P.R.
      Proc. Natl. Acad. Sci. U.S.A. 97:10567-10572(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ECOR 7.
    6. "Active-site mapping and site-specific mutagenesis of glycinamide ribonucleotide transformylase from Escherichia coli."
      Inglese J., Smith J.M., Benkovic S.J.
      Biochemistry 29:6678-6687(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVE SITE, PARTIAL PROTEIN SEQUENCE.
    7. "A rapid screen of active site mutants in glycinamide ribonucleotide transformylase."
      Warren M.S., Marolewski A.E., Benkovic S.J.
      Biochemistry 35:8855-8862(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    8. "Catalytic mechanism of Escherichia coli glycinamide ribonucleotide transformylase probed by site-directed mutagenesis and pH-dependent studies."
      Shim J.H., Benkovic S.J.
      Biochemistry 38:10024-10031(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS, ACTIVE SITE.
    9. "Crystal structure of glycinamide ribonucleotide transformylase from Escherichia coli at 3.0-A resolution. A target enzyme for chemotherapy."
      Chen P., Schulze-Gahmen U., Stura E.A., Inglese J., Johnson D.L., Marolewski A., Benkovic S.J., Wilson I.A.
      J. Mol. Biol. 227:283-292(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG.
    10. "Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase."
      Almassy R.J., Janson C.A., Kan C.-C., Hostomska Z.
      Proc. Natl. Acad. Sci. U.S.A. 89:6114-6118(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS.
    11. "A pH-dependent stabilization of an active site loop observed from low and high pH crystal structures of mutant monomeric glycinamide ribonucleotide transformylase at 1.8 to 1.9 A."
      Su Y., Yamashita M.M., Greasley S.E., Mullen C.A., Shim J.H., Jennings P.A., Benkovic S.J., Wilson I.A.
      J. Mol. Biol. 281:485-499(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANT ALA-70 IN COMPLEX WITH SUBSTRATE ANALOGS, SUBUNIT.
    12. "New insights into inhibitor design from the crystal structure and NMR studies of Escherichia coli GAR transformylase in complex with beta-GAR and 10-formyl-5,8,10-trideazafolic acid."
      Greasley S.E., Yamashita M.M., Cai H., Benkovic S.J., Boger D.L., Wilson I.A.
      Biochemistry 38:16783-16793(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS.

    Entry informationi

    Entry nameiPUR3_ECOLI
    AccessioniPrimary (citable) accession number: P08179
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3