P08178 (PUR5_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Synonyms:	purG
Ordered Locus Names:	b2499, JW2484

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741_B
Subunit structure	Homodimer.
Subcellular location	Cytoplasm HAMAP-Rule MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from direct assay Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.6

Chain

2 – 345

344

Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741_B

PRO_0000148211

Experimental info

Sequence conflict

12

1

A → D AA sequence Ref.5

Secondary structure

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345

Helix Strand Turn

Details...

Turn

7 – 9

3

Beta strand

10 – 12

3

Helix

18 – 25

8

Helix

27 – 31

5

Beta strand

38 – 40

3

Beta strand

42 – 50

9

Beta strand

56 – 66

11

Helix

70 – 76

7

Helix

83 – 95

13

Helix

96 – 98

3

Beta strand

101 – 113

13

Helix

116 – 133

18

Beta strand

136 – 144

9

Turn

146 – 148

3

Beta strand

154 – 165

12

Helix

166 – 168

3

Beta strand

180 – 185

6

Beta strand

187 – 189

3

Helix

194 – 203

10

Turn

208 – 210

3

Beta strand

212 – 217

6

Helix

218 – 223

6

Helix

230 – 239

10

Beta strand

244 – 247

4

Helix

252 – 256

5

Helix

257 – 259

3

Beta strand

265 – 269

5

Helix

271 – 273

3

Helix

278 – 287

10

Helix

291 – 297

7

Beta strand

302 – 308

7

Helix

310 – 312

3

Helix

313 – 321

9

Turn

322 – 324

3

Beta strand

327 – 335

9

Beta strand

340 – 343

4

Sequences

	Sequence		Length	Mass (Da)
	P08178 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8DA61375E8180401	FASTA	345	36,854
	10 20 30 40 50 60 MTDKTSLSYK DAGVDIDAGN ALVGRIKGVV KKTRRPEVMG GLGGFGALCA LPQKYREPVL 70 80 90 100 110 120 VSGTDGVGTK LRLAMDLKRH DTIGIDLVAM CVNDLVVQGA EPLFFLDYYA TGKLDVDTAS 130 140 150 160 170 180 AVISGIAEGC LQSGCSLVGG ETAEMPGMYH GEDYDVAGFC VGVVEKSEII DGSKVSDGDV 190 200 210 220 230 240 LIALGSSGPH SNGYSLVRKI LEVSGCDPQT TELDGKPLAD HLLAPTRIYV KSVLELIEKV 250 260 270 280 290 300 DVHAIAHLTG GGFWENIPRV LPDNTQAVID ESSWQWPEVF NWLQTAGNVE HHEMYRTFNC 310 320 330 340 GVGMIIALPA PEVDKALALL NANGENAWKI GIIKASDSEQ RVVIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12." Smith J.M., Daum H.A. III J. Biol. Chem. 261:10632-10636(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Purification and characterization of aminoimidazole ribonucleotide synthetase from Escherichia coli." Schrimsher J.L., Schendel F.J., Stubbe J., Smith J.M. Biochemistry 25:4366-4371(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[6]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12. Strain: K12 / EMG2.
[7]	"X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5-A resolution." Li C., Kappock T.J., Stubbe J., Weaver T.M., Ealick S.E. Structure 7:1155-1166(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M13747 Genomic DNA. Translation: AAA83898.1.
U00096 Genomic DNA. Translation: AAC75552.1.
AP009048 Genomic DNA. Translation: BAA16387.1.

PIR

AJECPC. A25955.

RefSeq

NP_416994.1. NC_000913.2.
YP_490727.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CLI	X-ray	2.50	A/B/C/D	2-345	[»]

ProteinModelPortal

P08178.

SMR

P08178. Positions 5-345.

ModBase

Search...

Protein-protein interaction databases

IntAct

P08178. 3 interactions.

STRING

511145.b2499.

Proteomic databases

PaxDb

P08178.

PRIDE

P08178.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC75552; AAC75552; b2499.
BAA16387; BAA16387; BAA16387.

GeneID

12932445.
946975.

KEGG

ecj:Y75_p2452.
eco:b2499.

PATRIC

32120387. VBIEscCol129921_2596.

Organism-specific databases

EchoBASE

EB0791.

EcoGene

EG10798. purM.

Phylogenomic databases

eggNOG

COG0150.

HOGENOM

HOG000229091.

KO

K01933.

OMA

IDMIAMN.

ProtClustDB

PRK05385.

Enzyme and pathway databases

BioCyc

EcoCyc:AIRS-MONOMER.
ECOL316407:JW2484-MONOMER.
MetaCyc:AIRS-MONOMER.

UniPathway

UPA00074; UER00129.

Gene expression databases

Genevestigator

P08178.

Family and domain databases

HAMAP

MF_00741_B. AIRS_B.

InterPro

IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]

Pfam

PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]

SUPFAM

SSF56042. AIR_synth_C. 1 hit.
SSF55326. PurM_N-like. 1 hit.

TIGRFAMs

TIGR00878. purM. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P08178.

Entry information

Entry name

PUR5_ECOLI

Accession

Primary (citable) accession number: P08178

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 126 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program