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P08178

- PUR5_ECOLI

UniProt

P08178 - PUR5_ECOLI

Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphoribosylformylglycinamidine cyclo-ligase activity Source: EcoCyc

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:AIRS-MONOMER.
    ECOL316407:JW2484-MONOMER.
    MetaCyc:AIRS-MONOMER.
    UniPathwayiUPA00074; UER00129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
    Alternative name(s):
    AIR synthase
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    Gene namesi
    Name:purM
    Synonyms:purG
    Ordered Locus Names:b2499, JW2484
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10798. purM.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 345344Phosphoribosylformylglycinamidine cyclo-ligasePRO_0000148211Add
    BLAST

    Proteomic databases

    PaxDbiP08178.
    PRIDEiP08178.

    Expressioni

    Gene expression databases

    GenevestigatoriP08178.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiP08178. 4 interactions.
    STRINGi511145.b2499.

    Structurei

    Secondary structure

    1
    345
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni7 – 93
    Beta strandi10 – 123
    Helixi18 – 258
    Helixi27 – 315
    Beta strandi38 – 403
    Beta strandi42 – 509
    Beta strandi56 – 6611
    Helixi70 – 767
    Helixi83 – 9513
    Helixi96 – 983
    Beta strandi101 – 11313
    Helixi116 – 13318
    Beta strandi136 – 1449
    Turni146 – 1483
    Beta strandi154 – 16512
    Helixi166 – 1683
    Beta strandi180 – 1856
    Beta strandi187 – 1893
    Helixi194 – 20310
    Turni208 – 2103
    Beta strandi212 – 2176
    Helixi218 – 2236
    Helixi230 – 23910
    Beta strandi244 – 2474
    Helixi252 – 2565
    Helixi257 – 2593
    Beta strandi265 – 2695
    Helixi271 – 2733
    Helixi278 – 28710
    Helixi291 – 2977
    Beta strandi302 – 3087
    Helixi310 – 3123
    Helixi313 – 3219
    Turni322 – 3243
    Beta strandi327 – 3359
    Beta strandi340 – 3434

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CLIX-ray2.50A/B/C/D2-345[»]
    ProteinModelPortaliP08178.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP08178.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AIR synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0150.
    HOGENOMiHOG000229091.
    KOiK01933.
    OMAiVIGKIEH.
    OrthoDBiEOG61CM1V.
    PhylomeDBiP08178.

    Family and domain databases

    Gene3Di3.30.1330.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPiMF_00741_B. AIRS_B.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsiTIGR00878. purM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P08178-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDKTSLSYK DAGVDIDAGN ALVGRIKGVV KKTRRPEVMG GLGGFGALCA    50
    LPQKYREPVL VSGTDGVGTK LRLAMDLKRH DTIGIDLVAM CVNDLVVQGA 100
    EPLFFLDYYA TGKLDVDTAS AVISGIAEGC LQSGCSLVGG ETAEMPGMYH 150
    GEDYDVAGFC VGVVEKSEII DGSKVSDGDV LIALGSSGPH SNGYSLVRKI 200
    LEVSGCDPQT TELDGKPLAD HLLAPTRIYV KSVLELIEKV DVHAIAHLTG 250
    GGFWENIPRV LPDNTQAVID ESSWQWPEVF NWLQTAGNVE HHEMYRTFNC 300
    GVGMIIALPA PEVDKALALL NANGENAWKI GIIKASDSEQ RVVIE 345
    Length:345
    Mass (Da):36,854
    Last modified:January 23, 2007 - v3
    Checksum:i8DA61375E8180401
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121A → D AA sequence (PubMed:3530323)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13747 Genomic DNA. Translation: AAA83898.1.
    U00096 Genomic DNA. Translation: AAC75552.1.
    AP009048 Genomic DNA. Translation: BAA16387.1.
    PIRiA25955. AJECPC.
    RefSeqiNP_416994.1. NC_000913.3.
    YP_490727.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75552; AAC75552; b2499.
    BAA16387; BAA16387; BAA16387.
    GeneIDi12932445.
    946975.
    KEGGiecj:Y75_p2452.
    eco:b2499.
    PATRICi32120387. VBIEscCol129921_2596.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13747 Genomic DNA. Translation: AAA83898.1 .
    U00096 Genomic DNA. Translation: AAC75552.1 .
    AP009048 Genomic DNA. Translation: BAA16387.1 .
    PIRi A25955. AJECPC.
    RefSeqi NP_416994.1. NC_000913.3.
    YP_490727.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CLI X-ray 2.50 A/B/C/D 2-345 [» ]
    ProteinModelPortali P08178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P08178. 4 interactions.
    STRINGi 511145.b2499.

    Proteomic databases

    PaxDbi P08178.
    PRIDEi P08178.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75552 ; AAC75552 ; b2499 .
    BAA16387 ; BAA16387 ; BAA16387 .
    GeneIDi 12932445.
    946975.
    KEGGi ecj:Y75_p2452.
    eco:b2499.
    PATRICi 32120387. VBIEscCol129921_2596.

    Organism-specific databases

    EchoBASEi EB0791.
    EcoGenei EG10798. purM.

    Phylogenomic databases

    eggNOGi COG0150.
    HOGENOMi HOG000229091.
    KOi K01933.
    OMAi VIGKIEH.
    OrthoDBi EOG61CM1V.
    PhylomeDBi P08178.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00129 .
    BioCyci EcoCyc:AIRS-MONOMER.
    ECOL316407:JW2484-MONOMER.
    MetaCyc:AIRS-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P08178.
    PROi P08178.

    Gene expression databases

    Genevestigatori P08178.

    Family and domain databases

    Gene3Di 3.30.1330.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPi MF_00741_B. AIRS_B.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsi TIGR00878. purM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12."
      Smith J.M., Daum H.A. III
      J. Biol. Chem. 261:10632-10636(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Purification and characterization of aminoimidazole ribonucleotide synthetase from Escherichia coli."
      Schrimsher J.L., Schendel F.J., Stubbe J., Smith J.M.
      Biochemistry 25:4366-4371(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-12.
      Strain: K12 / EMG2.
    7. "X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5-A resolution."
      Li C., Kappock T.J., Stubbe J., Weaver T.M., Ealick S.E.
      Structure 7:1155-1166(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

    Entry informationi

    Entry nameiPUR5_ECOLI
    AccessioniPrimary (citable) accession number: P08178
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3