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Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoribosylformylglycinamidine cyclo-ligase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:AIRS-MONOMER.
ECOL316407:JW2484-MONOMER.
MetaCyc:AIRS-MONOMER.
BRENDAi6.3.3.1. 2026.
UniPathwayiUPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:purM
Synonyms:purG
Ordered Locus Names:b2499, JW2484
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10798. purM.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001482112 – 345Phosphoribosylformylglycinamidine cyclo-ligaseAdd BLAST344

Proteomic databases

PaxDbiP08178.
PRIDEiP08178.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4261439. 18 interactors.
IntActiP08178. 4 interactors.
STRINGi511145.b2499.

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni7 – 9Combined sources3
Beta strandi10 – 12Combined sources3
Helixi18 – 25Combined sources8
Helixi27 – 31Combined sources5
Beta strandi38 – 40Combined sources3
Beta strandi42 – 50Combined sources9
Beta strandi56 – 66Combined sources11
Helixi70 – 76Combined sources7
Helixi83 – 95Combined sources13
Helixi96 – 98Combined sources3
Beta strandi101 – 113Combined sources13
Helixi116 – 133Combined sources18
Beta strandi136 – 144Combined sources9
Turni146 – 148Combined sources3
Beta strandi154 – 165Combined sources12
Helixi166 – 168Combined sources3
Beta strandi180 – 185Combined sources6
Beta strandi187 – 189Combined sources3
Helixi194 – 203Combined sources10
Turni208 – 210Combined sources3
Beta strandi212 – 217Combined sources6
Helixi218 – 223Combined sources6
Helixi230 – 239Combined sources10
Beta strandi244 – 247Combined sources4
Helixi252 – 256Combined sources5
Helixi257 – 259Combined sources3
Beta strandi265 – 269Combined sources5
Helixi271 – 273Combined sources3
Helixi278 – 287Combined sources10
Helixi291 – 297Combined sources7
Beta strandi302 – 308Combined sources7
Helixi310 – 312Combined sources3
Helixi313 – 321Combined sources9
Turni322 – 324Combined sources3
Beta strandi327 – 335Combined sources9
Beta strandi340 – 343Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLIX-ray2.50A/B/C/D2-345[»]
ProteinModelPortaliP08178.
SMRiP08178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08178.

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105CXB. Bacteria.
COG0150. LUCA.
HOGENOMiHOG000229091.
InParanoidiP08178.
KOiK01933.
OMAiLNRHDTV.
PhylomeDBiP08178.

Family and domain databases

CDDicd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKTSLSYK DAGVDIDAGN ALVGRIKGVV KKTRRPEVMG GLGGFGALCA
60 70 80 90 100
LPQKYREPVL VSGTDGVGTK LRLAMDLKRH DTIGIDLVAM CVNDLVVQGA
110 120 130 140 150
EPLFFLDYYA TGKLDVDTAS AVISGIAEGC LQSGCSLVGG ETAEMPGMYH
160 170 180 190 200
GEDYDVAGFC VGVVEKSEII DGSKVSDGDV LIALGSSGPH SNGYSLVRKI
210 220 230 240 250
LEVSGCDPQT TELDGKPLAD HLLAPTRIYV KSVLELIEKV DVHAIAHLTG
260 270 280 290 300
GGFWENIPRV LPDNTQAVID ESSWQWPEVF NWLQTAGNVE HHEMYRTFNC
310 320 330 340
GVGMIIALPA PEVDKALALL NANGENAWKI GIIKASDSEQ RVVIE
Length:345
Mass (Da):36,854
Last modified:January 23, 2007 - v3
Checksum:i8DA61375E8180401
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12A → D AA sequence (PubMed:3530323).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13747 Genomic DNA. Translation: AAA83898.1.
U00096 Genomic DNA. Translation: AAC75552.1.
AP009048 Genomic DNA. Translation: BAA16387.1.
PIRiA25955. AJECPC.
RefSeqiNP_416994.1. NC_000913.3.
WP_001336050.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75552; AAC75552; b2499.
BAA16387; BAA16387; BAA16387.
GeneIDi946975.
KEGGiecj:JW2484.
eco:b2499.
PATRICi32120387. VBIEscCol129921_2596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13747 Genomic DNA. Translation: AAA83898.1.
U00096 Genomic DNA. Translation: AAC75552.1.
AP009048 Genomic DNA. Translation: BAA16387.1.
PIRiA25955. AJECPC.
RefSeqiNP_416994.1. NC_000913.3.
WP_001336050.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLIX-ray2.50A/B/C/D2-345[»]
ProteinModelPortaliP08178.
SMRiP08178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261439. 18 interactors.
IntActiP08178. 4 interactors.
STRINGi511145.b2499.

Proteomic databases

PaxDbiP08178.
PRIDEiP08178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75552; AAC75552; b2499.
BAA16387; BAA16387; BAA16387.
GeneIDi946975.
KEGGiecj:JW2484.
eco:b2499.
PATRICi32120387. VBIEscCol129921_2596.

Organism-specific databases

EchoBASEiEB0791.
EcoGeneiEG10798. purM.

Phylogenomic databases

eggNOGiENOG4105CXB. Bacteria.
COG0150. LUCA.
HOGENOMiHOG000229091.
InParanoidiP08178.
KOiK01933.
OMAiLNRHDTV.
PhylomeDBiP08178.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00129.
BioCyciEcoCyc:AIRS-MONOMER.
ECOL316407:JW2484-MONOMER.
MetaCyc:AIRS-MONOMER.
BRENDAi6.3.3.1. 2026.

Miscellaneous databases

EvolutionaryTraceiP08178.
PROiP08178.

Family and domain databases

CDDicd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUR5_ECOLI
AccessioniPrimary (citable) accession number: P08178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.