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P08178

- PUR5_ECOLI

UniProt

P08178 - PUR5_ECOLI

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Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoribosylformylglycinamidine cyclo-ligase activity Source: EcoCyc

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:AIRS-MONOMER.
ECOL316407:JW2484-MONOMER.
MetaCyc:AIRS-MONOMER.
UniPathwayiUPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:purM
Synonyms:purG
Ordered Locus Names:b2499, JW2484
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10798. purM.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 345344Phosphoribosylformylglycinamidine cyclo-ligasePRO_0000148211Add
BLAST

Proteomic databases

PaxDbiP08178.
PRIDEiP08178.

Expressioni

Gene expression databases

GenevestigatoriP08178.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP08178. 4 interactions.
STRINGi511145.b2499.

Structurei

Secondary structure

1
345
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni7 – 93Combined sources
Beta strandi10 – 123Combined sources
Helixi18 – 258Combined sources
Helixi27 – 315Combined sources
Beta strandi38 – 403Combined sources
Beta strandi42 – 509Combined sources
Beta strandi56 – 6611Combined sources
Helixi70 – 767Combined sources
Helixi83 – 9513Combined sources
Helixi96 – 983Combined sources
Beta strandi101 – 11313Combined sources
Helixi116 – 13318Combined sources
Beta strandi136 – 1449Combined sources
Turni146 – 1483Combined sources
Beta strandi154 – 16512Combined sources
Helixi166 – 1683Combined sources
Beta strandi180 – 1856Combined sources
Beta strandi187 – 1893Combined sources
Helixi194 – 20310Combined sources
Turni208 – 2103Combined sources
Beta strandi212 – 2176Combined sources
Helixi218 – 2236Combined sources
Helixi230 – 23910Combined sources
Beta strandi244 – 2474Combined sources
Helixi252 – 2565Combined sources
Helixi257 – 2593Combined sources
Beta strandi265 – 2695Combined sources
Helixi271 – 2733Combined sources
Helixi278 – 28710Combined sources
Helixi291 – 2977Combined sources
Beta strandi302 – 3087Combined sources
Helixi310 – 3123Combined sources
Helixi313 – 3219Combined sources
Turni322 – 3243Combined sources
Beta strandi327 – 3359Combined sources
Beta strandi340 – 3434Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CLIX-ray2.50A/B/C/D2-345[»]
ProteinModelPortaliP08178.
SMRiP08178. Positions 5-345.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08178.

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.Curated

Phylogenomic databases

eggNOGiCOG0150.
HOGENOMiHOG000229091.
InParanoidiP08178.
KOiK01933.
OMAiVIGKIEH.
OrthoDBiEOG61CM1V.
PhylomeDBiP08178.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08178-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDKTSLSYK DAGVDIDAGN ALVGRIKGVV KKTRRPEVMG GLGGFGALCA
60 70 80 90 100
LPQKYREPVL VSGTDGVGTK LRLAMDLKRH DTIGIDLVAM CVNDLVVQGA
110 120 130 140 150
EPLFFLDYYA TGKLDVDTAS AVISGIAEGC LQSGCSLVGG ETAEMPGMYH
160 170 180 190 200
GEDYDVAGFC VGVVEKSEII DGSKVSDGDV LIALGSSGPH SNGYSLVRKI
210 220 230 240 250
LEVSGCDPQT TELDGKPLAD HLLAPTRIYV KSVLELIEKV DVHAIAHLTG
260 270 280 290 300
GGFWENIPRV LPDNTQAVID ESSWQWPEVF NWLQTAGNVE HHEMYRTFNC
310 320 330 340
GVGMIIALPA PEVDKALALL NANGENAWKI GIIKASDSEQ RVVIE
Length:345
Mass (Da):36,854
Last modified:January 23, 2007 - v3
Checksum:i8DA61375E8180401
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121A → D AA sequence (PubMed:3530323)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13747 Genomic DNA. Translation: AAA83898.1.
U00096 Genomic DNA. Translation: AAC75552.1.
AP009048 Genomic DNA. Translation: BAA16387.1.
PIRiA25955. AJECPC.
RefSeqiNP_416994.1. NC_000913.3.
YP_490727.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75552; AAC75552; b2499.
BAA16387; BAA16387; BAA16387.
GeneIDi12932445.
946975.
KEGGiecj:Y75_p2452.
eco:b2499.
PATRICi32120387. VBIEscCol129921_2596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13747 Genomic DNA. Translation: AAA83898.1 .
U00096 Genomic DNA. Translation: AAC75552.1 .
AP009048 Genomic DNA. Translation: BAA16387.1 .
PIRi A25955. AJECPC.
RefSeqi NP_416994.1. NC_000913.3.
YP_490727.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CLI X-ray 2.50 A/B/C/D 2-345 [» ]
ProteinModelPortali P08178.
SMRi P08178. Positions 5-345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P08178. 4 interactions.
STRINGi 511145.b2499.

Proteomic databases

PaxDbi P08178.
PRIDEi P08178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75552 ; AAC75552 ; b2499 .
BAA16387 ; BAA16387 ; BAA16387 .
GeneIDi 12932445.
946975.
KEGGi ecj:Y75_p2452.
eco:b2499.
PATRICi 32120387. VBIEscCol129921_2596.

Organism-specific databases

EchoBASEi EB0791.
EcoGenei EG10798. purM.

Phylogenomic databases

eggNOGi COG0150.
HOGENOMi HOG000229091.
InParanoidi P08178.
KOi K01933.
OMAi VIGKIEH.
OrthoDBi EOG61CM1V.
PhylomeDBi P08178.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00129 .
BioCyci EcoCyc:AIRS-MONOMER.
ECOL316407:JW2484-MONOMER.
MetaCyc:AIRS-MONOMER.

Miscellaneous databases

EvolutionaryTracei P08178.
PROi P08178.

Gene expression databases

Genevestigatori P08178.

Family and domain databases

Gene3Di 3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPi MF_00741. AIRS.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsi TIGR00878. purM. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12."
    Smith J.M., Daum H.A. III
    J. Biol. Chem. 261:10632-10636(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Purification and characterization of aminoimidazole ribonucleotide synthetase from Escherichia coli."
    Schrimsher J.L., Schendel F.J., Stubbe J., Smith J.M.
    Biochemistry 25:4366-4371(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
  6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-12.
    Strain: K12 / EMG2.
  7. "X-ray crystal structure of aminoimidazole ribonucleotide synthetase (PurM), from the Escherichia coli purine biosynthetic pathway at 2.5-A resolution."
    Li C., Kappock T.J., Stubbe J., Weaver T.M., Ealick S.E.
    Structure 7:1155-1166(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiPUR5_ECOLI
AccessioniPrimary (citable) accession number: P08178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3