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Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoribosylformylglycinamidine cyclo-ligase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:AIRS-MONOMER
MetaCyc:AIRS-MONOMER
BRENDAi6.3.3.1 2026
UniPathwayiUPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:purM
Synonyms:purG
Ordered Locus Names:b2499, JW2484
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10798 purM

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001482112 – 345Phosphoribosylformylglycinamidine cyclo-ligaseAdd BLAST344

Proteomic databases

PaxDbiP08178
PRIDEiP08178

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4261439, 54 interactors
IntActiP08178, 4 interactors
STRINGi316385.ECDH10B_2665

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni7 – 9Combined sources3
Beta strandi10 – 12Combined sources3
Helixi18 – 25Combined sources8
Helixi27 – 31Combined sources5
Beta strandi38 – 40Combined sources3
Beta strandi42 – 50Combined sources9
Beta strandi56 – 66Combined sources11
Helixi70 – 76Combined sources7
Helixi83 – 95Combined sources13
Helixi96 – 98Combined sources3
Beta strandi101 – 113Combined sources13
Helixi116 – 133Combined sources18
Beta strandi136 – 144Combined sources9
Turni146 – 148Combined sources3
Beta strandi154 – 165Combined sources12
Helixi166 – 168Combined sources3
Beta strandi180 – 185Combined sources6
Beta strandi187 – 189Combined sources3
Helixi194 – 203Combined sources10
Turni208 – 210Combined sources3
Beta strandi212 – 217Combined sources6
Helixi218 – 223Combined sources6
Helixi230 – 239Combined sources10
Beta strandi244 – 247Combined sources4
Helixi252 – 256Combined sources5
Helixi257 – 259Combined sources3
Beta strandi265 – 269Combined sources5
Helixi271 – 273Combined sources3
Helixi278 – 287Combined sources10
Helixi291 – 297Combined sources7
Beta strandi302 – 308Combined sources7
Helixi310 – 312Combined sources3
Helixi313 – 321Combined sources9
Turni322 – 324Combined sources3
Beta strandi327 – 335Combined sources9
Beta strandi340 – 343Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLIX-ray2.50A/B/C/D2-345[»]
ProteinModelPortaliP08178
SMRiP08178
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08178

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105CXB Bacteria
COG0150 LUCA
HOGENOMiHOG000229091
InParanoidiP08178
KOiK01933
OMAiNHCVNDI
PhylomeDBiP08178

Family and domain databases

CDDicd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
InterProiView protein in InterPro
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
PANTHERiPTHR10520 PTHR10520, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
SUPFAMiSSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00878 purM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKTSLSYK DAGVDIDAGN ALVGRIKGVV KKTRRPEVMG GLGGFGALCA
60 70 80 90 100
LPQKYREPVL VSGTDGVGTK LRLAMDLKRH DTIGIDLVAM CVNDLVVQGA
110 120 130 140 150
EPLFFLDYYA TGKLDVDTAS AVISGIAEGC LQSGCSLVGG ETAEMPGMYH
160 170 180 190 200
GEDYDVAGFC VGVVEKSEII DGSKVSDGDV LIALGSSGPH SNGYSLVRKI
210 220 230 240 250
LEVSGCDPQT TELDGKPLAD HLLAPTRIYV KSVLELIEKV DVHAIAHLTG
260 270 280 290 300
GGFWENIPRV LPDNTQAVID ESSWQWPEVF NWLQTAGNVE HHEMYRTFNC
310 320 330 340
GVGMIIALPA PEVDKALALL NANGENAWKI GIIKASDSEQ RVVIE
Length:345
Mass (Da):36,854
Last modified:January 23, 2007 - v3
Checksum:i8DA61375E8180401
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12A → D AA sequence (PubMed:3530323).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13747 Genomic DNA Translation: AAA83898.1
U00096 Genomic DNA Translation: AAC75552.1
AP009048 Genomic DNA Translation: BAA16387.1
PIRiA25955 AJECPC
RefSeqiNP_416994.1, NC_000913.3
WP_001336050.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75552; AAC75552; b2499
BAA16387; BAA16387; BAA16387
GeneIDi946975
KEGGiecj:JW2484
eco:b2499
PATRICifig|1411691.4.peg.4239

Entry informationi

Entry nameiPUR5_ECOLI
AccessioniPrimary (citable) accession number: P08178
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health