P08173 (ACM4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Muscarinic acetylcholine receptor M4

Gene names

Name:

CHRM4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Inferred from electronic annotation. Source: Compara cell proliferation Traceable author statement PubMed 2739737. Source: ProtInc
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled acetylcholine receptor activity Traceable author statement PubMed 9603968. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Muscarinic acetylcholine receptor M4		PRO_0000069037
Regions
	Topological domain	1 – 31	31	Extracellular By similarity
	Transmembrane	32 – 54	23	Helical; Name=1; By similarity
	Topological domain	55 – 68	14	Cytoplasmic By similarity
	Transmembrane	69 – 89	21	Helical; Name=2; By similarity
	Topological domain	90 – 106	17	Extracellular By similarity
	Transmembrane	107 – 128	22	Helical; Name=3; By similarity
	Topological domain	129 – 148	20	Cytoplasmic By similarity
	Transmembrane	149 – 171	23	Helical; Name=4; By similarity
	Topological domain	172 – 193	22	Extracellular By similarity
	Transmembrane	194 – 216	23	Helical; Name=5; By similarity
	Topological domain	217 – 401	185	Cytoplasmic By similarity
	Transmembrane	402 – 422	21	Helical; Name=6; By similarity
	Topological domain	423 – 436	14	Extracellular By similarity
	Transmembrane	437 – 456	20	Helical; Name=7; By similarity
	Topological domain	457 – 479	23	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	459	1	Phosphothreonine Potential
	Modified residue	463	1	Phosphothreonine Potential
	Modified residue	477	1	Phosphothreonine Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Disulfide bond	105 ↔ 185		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P08173 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 9C1B05B359A64289	FASTA	479	53,049
	10 20 30 40 50 60 MANFTPVNGS SGNQSVRLVT SSSHNRYETV EMVFIATVTG SLSLVTVVGN ILVMLSIKVN 70 80 90 100 110 120 RQLQTVNNYF LFSLACADLI IGAFSMNLYT VYIIKGYWPL GAVVCDLWLA LDYVVSNASV 130 140 150 160 170 180 MNLLIISFDR YFCVTKPLTY PARRTTKMAG LMIAAAWVLS FVLWAPAILF WQFVVGKRTV 190 200 210 220 230 240 PDNQCFIQFL SNPAVTFGTA IAAFYLPVVI MTVLYIHISL ASRSRVHKHR PEGPKEKKAK 250 260 270 280 290 300 TLAFLKSPLM KQSVKKPPPG EAAREELRNG KLEEAPPPAL PPPPRPVADK DTSNESSSGS 310 320 330 340 350 360 ATQNTKERPA TELSTTEATT PAMPAPPLQP RALNPASRWS KIQIVTKQTG NECVTAIEIV 370 380 390 400 410 420 PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR TIFAILLAFI LTWTPYNVMV 430 440 450 460 470 LVNTFCQSCI PDTVWSIGYW LCYVNSTINP ACYALCNATF KKTFRHLLLC QYRNIGTAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a family of muscarinic acetylcholine receptor genes." Bonner T.I., Buckley N.J., Young A.C., Brann M.R. Science 237:527-532(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes." Bonner T.I., Young A.C., Brann M.R., Buckley N.J. Neuron 1:403-410(1988) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 258 AND 261-266.
[3]	"Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors." Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J. EMBO J. 6:3923-3929(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16405 Genomic DNA. Translation: AAA51571.2. X15265 Genomic DNA. Translation: CAA33336.1. AF498918 mRNA. Translation: AAM18941.1. CH471064 Genomic DNA. Translation: EAW68004.1. BC095546 mRNA. Translation: AAH95546.1. BC119775 mRNA. Translation: AAI19776.1. BC119817 mRNA. Translation: AAI19818.1. BC137535 mRNA. Translation: AAI37536.1.
IPI	IPI00027474.
PIR	S10127.
RefSeq	NP_000732.2. NM_000741.2.
UniGene	Hs.248100.
3D structure databases
ProteinModelPortal	P08173.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000409378.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P08173.
Polymorphism databases
DMDM	23503039.
Proteomic databases
PaxDb	P08173.
PRIDE	P08173.
Protocols and materials databases
DNASU	1132.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000433765; ENSP00000409378; ENSG00000180720.
GeneID	1132.
KEGG	hsa:1132.
UCSC	uc001nct.1. human.
Organism-specific databases
CTD	1132.
GeneCards	GC11M046406.
HGNC	HGNC:1953. CHRM4.
HPA	CAB022444.
MIM	118495. gene.
neXtProt	NX_P08173.
PharmGKB	PA26485.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG250863.
HOGENOM	HOG000231484.
HOVERGEN	HBG105720.
KO	K04132.
OMA	CIPDTVW.
OrthoDB	EOG41JZCC.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P08173.
CleanEx	HS_CHRM4.
Genevestigator	P08173.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001432. Musac_M4_rcpt. IPR000995. Musac_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00541. MUSCRINICM4R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P08173.
ChEMBL	CHEMBL1821.
DrugBank	DB00572. Atropine. DB00767. Benzquinamide. DB00785. Cryptenamine. DB00725. Homatropine Methylbromide. DB01403. Methotrimeprazine. DB00340. Metixene. DB00334. Olanzapine. DB00387. Procyclidine. DB00420. Promazine. DB01069. Promethazine. DB00777. Propiomazine. DB00372. Thiethylperazine. DB00809. Tropicamide.
GenomeRNAi	1132.
NextBio	4708.
SOURCE	Search...

Entry information

Entry name

ACM4_HUMAN

Accession

Primary (citable) accession number: P08173
Secondary accession number(s): B2RPP4, Q0VD60, Q4VBK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	September 19, 2002
Last modified:	May 1, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.