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P08173

- ACM4_HUMAN

UniProt

P08173 - ACM4_HUMAN

Protein

Muscarinic acetylcholine receptor M4

Gene

CHRM4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (19 Sep 2002)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

    GO - Molecular functioni

    1. G-protein coupled acetylcholine receptor activity Source: ProtInc

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: Ensembl
    2. cell proliferation Source: ProtInc
    3. cell surface receptor signaling pathway Source: ProtInc
    4. G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
    5. regulation of locomotion Source: InterPro
    6. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M4
    Gene namesi
    Name:CHRM4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:1953. CHRM4.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of plasma membrane Source: ProtInc
    3. plasma membrane Source: Reactome
    4. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26485.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Muscarinic acetylcholine receptor M4PRO_0000069037Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi13 – 131N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi105 ↔ 185PROSITE-ProRule annotation
    Modified residuei459 – 4591PhosphothreonineSequence Analysis
    Modified residuei463 – 4631PhosphothreonineSequence Analysis
    Modified residuei477 – 4771PhosphothreonineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP08173.
    PRIDEiP08173.

    PTM databases

    PhosphoSiteiP08173.

    Expressioni

    Gene expression databases

    BgeeiP08173.
    CleanExiHS_CHRM4.
    GenevestigatoriP08173.

    Organism-specific databases

    HPAiCAB022444.

    Interactioni

    Protein-protein interaction databases

    BioGridi107554. 1 interaction.
    STRINGi9606.ENSP00000409378.

    Structurei

    3D structure databases

    ProteinModelPortaliP08173.
    SMRiP08173. Positions 29-469.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3131ExtracellularBy similarityAdd
    BLAST
    Topological domaini55 – 6814CytoplasmicBy similarityAdd
    BLAST
    Topological domaini90 – 10617ExtracellularBy similarityAdd
    BLAST
    Topological domaini129 – 14820CytoplasmicBy similarityAdd
    BLAST
    Topological domaini172 – 19322ExtracellularBy similarityAdd
    BLAST
    Topological domaini217 – 401185CytoplasmicBy similarityAdd
    BLAST
    Topological domaini423 – 43614ExtracellularBy similarityAdd
    BLAST
    Topological domaini457 – 47923CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei32 – 5423Helical; Name=1By similarityAdd
    BLAST
    Transmembranei69 – 8921Helical; Name=2By similarityAdd
    BLAST
    Transmembranei107 – 12822Helical; Name=3By similarityAdd
    BLAST
    Transmembranei149 – 17123Helical; Name=4By similarityAdd
    BLAST
    Transmembranei194 – 21623Helical; Name=5By similarityAdd
    BLAST
    Transmembranei402 – 42221Helical; Name=6By similarityAdd
    BLAST
    Transmembranei437 – 45620Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    KOiK04132.
    OMAiCIPDTVW.
    OrthoDBiEOG7V49Z7.
    PhylomeDBiP08173.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001432. Musac_Ach_M4_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PANTHERiPTHR24249:SF60. PTHR24249:SF60. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00541. MUSCRINICM4R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P08173-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANFTPVNGS SGNQSVRLVT SSSHNRYETV EMVFIATVTG SLSLVTVVGN    50
    ILVMLSIKVN RQLQTVNNYF LFSLACADLI IGAFSMNLYT VYIIKGYWPL 100
    GAVVCDLWLA LDYVVSNASV MNLLIISFDR YFCVTKPLTY PARRTTKMAG 150
    LMIAAAWVLS FVLWAPAILF WQFVVGKRTV PDNQCFIQFL SNPAVTFGTA 200
    IAAFYLPVVI MTVLYIHISL ASRSRVHKHR PEGPKEKKAK TLAFLKSPLM 250
    KQSVKKPPPG EAAREELRNG KLEEAPPPAL PPPPRPVADK DTSNESSSGS 300
    ATQNTKERPA TELSTTEATT PAMPAPPLQP RALNPASRWS KIQIVTKQTG 350
    NECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR 400
    TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PDTVWSIGYW LCYVNSTINP 450
    ACYALCNATF KKTFRHLLLC QYRNIGTAR 479
    Length:479
    Mass (Da):53,049
    Last modified:September 19, 2002 - v2
    Checksum:i9C1B05B359A64289
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16405 Genomic DNA. Translation: AAA51571.2.
    X15265 Genomic DNA. Translation: CAA33336.1.
    AF498918 mRNA. Translation: AAM18941.1.
    CH471064 Genomic DNA. Translation: EAW68004.1.
    BC095546 mRNA. Translation: AAH95546.1.
    BC119775 mRNA. Translation: AAI19776.1.
    BC119817 mRNA. Translation: AAI19818.1.
    BC137535 mRNA. Translation: AAI37536.1.
    CCDSiCCDS44581.1.
    PIRiS10127.
    RefSeqiNP_000732.2. NM_000741.2.
    UniGeneiHs.248100.

    Genome annotation databases

    EnsembliENST00000433765; ENSP00000409378; ENSG00000180720.
    GeneIDi1132.
    KEGGihsa:1132.
    UCSCiuc001nct.1. human.

    Polymorphism databases

    DMDMi23503039.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M16405 Genomic DNA. Translation: AAA51571.2 .
    X15265 Genomic DNA. Translation: CAA33336.1 .
    AF498918 mRNA. Translation: AAM18941.1 .
    CH471064 Genomic DNA. Translation: EAW68004.1 .
    BC095546 mRNA. Translation: AAH95546.1 .
    BC119775 mRNA. Translation: AAI19776.1 .
    BC119817 mRNA. Translation: AAI19818.1 .
    BC137535 mRNA. Translation: AAI37536.1 .
    CCDSi CCDS44581.1.
    PIRi S10127.
    RefSeqi NP_000732.2. NM_000741.2.
    UniGenei Hs.248100.

    3D structure databases

    ProteinModelPortali P08173.
    SMRi P08173. Positions 29-469.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107554. 1 interaction.
    STRINGi 9606.ENSP00000409378.

    Chemistry

    BindingDBi P08173.
    ChEMBLi CHEMBL2094109.
    DrugBanki DB00572. Atropine.
    DB00767. Benzquinamide.
    DB00785. Cryptenamine.
    DB00725. Homatropine Methylbromide.
    DB01403. Methotrimeprazine.
    DB00340. Metixene.
    DB00334. Olanzapine.
    DB00387. Procyclidine.
    DB00420. Promazine.
    DB01069. Promethazine.
    DB00777. Propiomazine.
    DB00372. Thiethylperazine.
    DB00809. Tropicamide.
    GuidetoPHARMACOLOGYi 16.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P08173.

    Polymorphism databases

    DMDMi 23503039.

    Proteomic databases

    PaxDbi P08173.
    PRIDEi P08173.

    Protocols and materials databases

    DNASUi 1132.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000433765 ; ENSP00000409378 ; ENSG00000180720 .
    GeneIDi 1132.
    KEGGi hsa:1132.
    UCSCi uc001nct.1. human.

    Organism-specific databases

    CTDi 1132.
    GeneCardsi GC11M046406.
    HGNCi HGNC:1953. CHRM4.
    HPAi CAB022444.
    MIMi 118495. gene.
    neXtProti NX_P08173.
    PharmGKBi PA26485.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG250863.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    KOi K04132.
    OMAi CIPDTVW.
    OrthoDBi EOG7V49Z7.
    PhylomeDBi P08173.
    TreeFami TF320495.

    Enzyme and pathway databases

    Reactomei REACT_16943. Muscarinic acetylcholine receptors.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    GeneWikii Muscarinic_acetylcholine_receptor_M4.
    GenomeRNAii 1132.
    NextBioi 4708.
    PROi P08173.
    SOURCEi Search...

    Gene expression databases

    Bgeei P08173.
    CleanExi HS_CHRM4.
    Genevestigatori P08173.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001432. Musac_Ach_M4_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    PANTHERi PTHR24249:SF60. PTHR24249:SF60. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00541. MUSCRINICM4R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a family of muscarinic acetylcholine receptor genes."
      Bonner T.I., Buckley N.J., Young A.C., Brann M.R.
      Science 237:527-532(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes."
      Bonner T.I., Young A.C., Brann M.R., Buckley N.J.
      Neuron 1:403-410(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 258 AND 261-266.
    3. "Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors."
      Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J.
      EMBO J. 6:3923-3929(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiACM4_HUMAN
    AccessioniPrimary (citable) accession number: P08173
    Secondary accession number(s): B2RPP4, Q0VD60, Q4VBK7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: September 19, 2002
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3