Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Muscarinic acetylcholine receptor M4

Gene

CHRM4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functioni

  • G-protein coupled acetylcholine receptor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
REACT_19231. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Gene namesi
Name:CHRM4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:1953. CHRM4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131ExtracellularBy similarityAdd
BLAST
Transmembranei32 – 5423Helical; Name=1By similarityAdd
BLAST
Topological domaini55 – 6814CytoplasmicBy similarityAdd
BLAST
Transmembranei69 – 8921Helical; Name=2By similarityAdd
BLAST
Topological domaini90 – 10617ExtracellularBy similarityAdd
BLAST
Transmembranei107 – 12822Helical; Name=3By similarityAdd
BLAST
Topological domaini129 – 14820CytoplasmicBy similarityAdd
BLAST
Transmembranei149 – 17123Helical; Name=4By similarityAdd
BLAST
Topological domaini172 – 19322ExtracellularBy similarityAdd
BLAST
Transmembranei194 – 21623Helical; Name=5By similarityAdd
BLAST
Topological domaini217 – 401185CytoplasmicBy similarityAdd
BLAST
Transmembranei402 – 42221Helical; Name=6By similarityAdd
BLAST
Topological domaini423 – 43614ExtracellularBy similarityAdd
BLAST
Transmembranei437 – 45620Helical; Name=7By similarityAdd
BLAST
Topological domaini457 – 47923CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
  • postsynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26485.

Chemistry

DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00835. Brompheniramine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00363. Clozapine.
DB00785. Cryptenamine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB01142. Doxepin.
DB06702. Fesoterodine.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB01625. Isopropamide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB01403. Methotrimeprazine.
DB00340. Metixene.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB00715. Paroxetine.
DB00454. Pethidine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB01036. Tolterodine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB00246. Ziprasidone.

Polymorphism and mutation databases

BioMutaiCHRM4.
DMDMi23503039.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Muscarinic acetylcholine receptor M4PRO_0000069037Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence Analysis
Glycosylationi13 – 131N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi105 ↔ 185PROSITE-ProRule annotation
Modified residuei459 – 4591PhosphothreonineSequence Analysis
Modified residuei463 – 4631PhosphothreonineSequence Analysis
Modified residuei477 – 4771PhosphothreonineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08173.
PRIDEiP08173.

PTM databases

PhosphoSiteiP08173.

Expressioni

Gene expression databases

BgeeiP08173.
CleanExiHS_CHRM4.
GenevestigatoriP08173.

Organism-specific databases

HPAiCAB022444.

Interactioni

Protein-protein interaction databases

BioGridi107554. 1 interaction.
STRINGi9606.ENSP00000409378.

Structurei

3D structure databases

ProteinModelPortaliP08173.
SMRiP08173. Positions 28-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08173.
KOiK04132.
OMAiCIPDTVW.
OrthoDBiEOG7V49Z7.
PhylomeDBiP08173.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFTPVNGS SGNQSVRLVT SSSHNRYETV EMVFIATVTG SLSLVTVVGN
60 70 80 90 100
ILVMLSIKVN RQLQTVNNYF LFSLACADLI IGAFSMNLYT VYIIKGYWPL
110 120 130 140 150
GAVVCDLWLA LDYVVSNASV MNLLIISFDR YFCVTKPLTY PARRTTKMAG
160 170 180 190 200
LMIAAAWVLS FVLWAPAILF WQFVVGKRTV PDNQCFIQFL SNPAVTFGTA
210 220 230 240 250
IAAFYLPVVI MTVLYIHISL ASRSRVHKHR PEGPKEKKAK TLAFLKSPLM
260 270 280 290 300
KQSVKKPPPG EAAREELRNG KLEEAPPPAL PPPPRPVADK DTSNESSSGS
310 320 330 340 350
ATQNTKERPA TELSTTEATT PAMPAPPLQP RALNPASRWS KIQIVTKQTG
360 370 380 390 400
NECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR
410 420 430 440 450
TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PDTVWSIGYW LCYVNSTINP
460 470
ACYALCNATF KKTFRHLLLC QYRNIGTAR
Length:479
Mass (Da):53,049
Last modified:September 19, 2002 - v2
Checksum:i9C1B05B359A64289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16405 Genomic DNA. Translation: AAA51571.2.
X15265 Genomic DNA. Translation: CAA33336.1.
AF498918 mRNA. Translation: AAM18941.1.
CH471064 Genomic DNA. Translation: EAW68004.1.
BC095546 mRNA. Translation: AAH95546.1.
BC119775 mRNA. Translation: AAI19776.1.
BC119817 mRNA. Translation: AAI19818.1.
BC137535 mRNA. Translation: AAI37536.1.
CCDSiCCDS44581.1.
PIRiS10127.
RefSeqiNP_000732.2. NM_000741.3.
UniGeneiHs.248100.

Genome annotation databases

EnsembliENST00000433765; ENSP00000409378; ENSG00000180720.
GeneIDi1132.
KEGGihsa:1132.
UCSCiuc001nct.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16405 Genomic DNA. Translation: AAA51571.2.
X15265 Genomic DNA. Translation: CAA33336.1.
AF498918 mRNA. Translation: AAM18941.1.
CH471064 Genomic DNA. Translation: EAW68004.1.
BC095546 mRNA. Translation: AAH95546.1.
BC119775 mRNA. Translation: AAI19776.1.
BC119817 mRNA. Translation: AAI19818.1.
BC137535 mRNA. Translation: AAI37536.1.
CCDSiCCDS44581.1.
PIRiS10127.
RefSeqiNP_000732.2. NM_000741.3.
UniGeneiHs.248100.

3D structure databases

ProteinModelPortaliP08173.
SMRiP08173. Positions 28-469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107554. 1 interaction.
STRINGi9606.ENSP00000409378.

Chemistry

BindingDBiP08173.
ChEMBLiCHEMBL2111445.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00835. Brompheniramine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00363. Clozapine.
DB00785. Cryptenamine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB01142. Doxepin.
DB06702. Fesoterodine.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB01625. Isopropamide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB01403. Methotrimeprazine.
DB00340. Metixene.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB00715. Paroxetine.
DB00454. Pethidine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB01036. Tolterodine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi16.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP08173.

Polymorphism and mutation databases

BioMutaiCHRM4.
DMDMi23503039.

Proteomic databases

PaxDbiP08173.
PRIDEiP08173.

Protocols and materials databases

DNASUi1132.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000433765; ENSP00000409378; ENSG00000180720.
GeneIDi1132.
KEGGihsa:1132.
UCSCiuc001nct.1. human.

Organism-specific databases

CTDi1132.
GeneCardsiGC11M046406.
HGNCiHGNC:1953. CHRM4.
HPAiCAB022444.
MIMi118495. gene.
neXtProtiNX_P08173.
PharmGKBiPA26485.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08173.
KOiK04132.
OMAiCIPDTVW.
OrthoDBiEOG7V49Z7.
PhylomeDBiP08173.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiREACT_16943. Muscarinic acetylcholine receptors.
REACT_19231. G alpha (i) signalling events.

Miscellaneous databases

GeneWikiiMuscarinic_acetylcholine_receptor_M4.
GenomeRNAii1132.
NextBioi4708.
PROiP08173.
SOURCEiSearch...

Gene expression databases

BgeeiP08173.
CleanExiHS_CHRM4.
GenevestigatoriP08173.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a family of muscarinic acetylcholine receptor genes."
    Bonner T.I., Buckley N.J., Young A.C., Brann M.R.
    Science 237:527-532(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes."
    Bonner T.I., Young A.C., Brann M.R., Buckley N.J.
    Neuron 1:403-410(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 258 AND 261-266.
  3. "Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors."
    Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J.
    EMBO J. 6:3923-3929(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiACM4_HUMAN
AccessioniPrimary (citable) accession number: P08173
Secondary accession number(s): B2RPP4, Q0VD60, Q4VBK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: September 19, 2002
Last modified: April 29, 2015
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.