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P08173 (ACM4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M4
Gene names
Name:CHRM4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Muscarinic acetylcholine receptor M4
PRO_0000069037

Regions

Topological domain1 – 3131Extracellular By similarity
Transmembrane32 – 5423Helical; Name=1; By similarity
Topological domain55 – 6814Cytoplasmic By similarity
Transmembrane69 – 8921Helical; Name=2; By similarity
Topological domain90 – 10617Extracellular By similarity
Transmembrane107 – 12822Helical; Name=3; By similarity
Topological domain129 – 14820Cytoplasmic By similarity
Transmembrane149 – 17123Helical; Name=4; By similarity
Topological domain172 – 19322Extracellular By similarity
Transmembrane194 – 21623Helical; Name=5; By similarity
Topological domain217 – 401185Cytoplasmic By similarity
Transmembrane402 – 42221Helical; Name=6; By similarity
Topological domain423 – 43614Extracellular By similarity
Transmembrane437 – 45620Helical; Name=7; By similarity
Topological domain457 – 47923Cytoplasmic By similarity

Amino acid modifications

Modified residue4591Phosphothreonine Potential
Modified residue4631Phosphothreonine Potential
Modified residue4771Phosphothreonine Potential
Glycosylation81N-linked (GlcNAc...) Potential
Glycosylation131N-linked (GlcNAc...) Potential
Disulfide bond105 ↔ 185 By similarity

Sequences

Sequence LengthMass (Da)Tools
P08173 [UniParc].

Last modified September 19, 2002. Version 2.
Checksum: 9C1B05B359A64289

FASTA47953,049
        10         20         30         40         50         60 
MANFTPVNGS SGNQSVRLVT SSSHNRYETV EMVFIATVTG SLSLVTVVGN ILVMLSIKVN 

        70         80         90        100        110        120 
RQLQTVNNYF LFSLACADLI IGAFSMNLYT VYIIKGYWPL GAVVCDLWLA LDYVVSNASV 

       130        140        150        160        170        180 
MNLLIISFDR YFCVTKPLTY PARRTTKMAG LMIAAAWVLS FVLWAPAILF WQFVVGKRTV 

       190        200        210        220        230        240 
PDNQCFIQFL SNPAVTFGTA IAAFYLPVVI MTVLYIHISL ASRSRVHKHR PEGPKEKKAK 

       250        260        270        280        290        300 
TLAFLKSPLM KQSVKKPPPG EAAREELRNG KLEEAPPPAL PPPPRPVADK DTSNESSSGS 

       310        320        330        340        350        360 
ATQNTKERPA TELSTTEATT PAMPAPPLQP RALNPASRWS KIQIVTKQTG NECVTAIEIV 

       370        380        390        400        410        420 
PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR TIFAILLAFI LTWTPYNVMV 

       430        440        450        460        470 
LVNTFCQSCI PDTVWSIGYW LCYVNSTINP ACYALCNATF KKTFRHLLLC QYRNIGTAR 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a family of muscarinic acetylcholine receptor genes."
Bonner T.I., Buckley N.J., Young A.C., Brann M.R.
Science 237:527-532(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes."
Bonner T.I., Young A.C., Brann M.R., Buckley N.J.
Neuron 1:403-410(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 258 AND 261-266.
[3]"Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors."
Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J., Capon D.J.
EMBO J. 6:3923-3929(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16405 Genomic DNA. Translation: AAA51571.2.
X15265 Genomic DNA. Translation: CAA33336.1.
AF498918 mRNA. Translation: AAM18941.1.
CH471064 Genomic DNA. Translation: EAW68004.1.
BC095546 mRNA. Translation: AAH95546.1.
BC119775 mRNA. Translation: AAI19776.1.
BC119817 mRNA. Translation: AAI19818.1.
BC137535 mRNA. Translation: AAI37536.1.
CCDSCCDS44581.1.
PIRS10127.
RefSeqNP_000732.2. NM_000741.2.
UniGeneHs.248100.

3D structure databases

ProteinModelPortalP08173.
SMRP08173. Positions 29-469.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107554. 1 interaction.
STRING9606.ENSP00000409378.

Chemistry

BindingDBP08173.
ChEMBLCHEMBL2094109.
DrugBankDB00572. Atropine.
DB00767. Benzquinamide.
DB00785. Cryptenamine.
DB00725. Homatropine Methylbromide.
DB01403. Methotrimeprazine.
DB00340. Metixene.
DB00334. Olanzapine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB00372. Thiethylperazine.
DB00809. Tropicamide.
GuidetoPHARMACOLOGY16.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP08173.

Polymorphism databases

DMDM23503039.

Proteomic databases

PaxDbP08173.
PRIDEP08173.

Protocols and materials databases

DNASU1132.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000433765; ENSP00000409378; ENSG00000180720.
GeneID1132.
KEGGhsa:1132.
UCSCuc001nct.1. human.

Organism-specific databases

CTD1132.
GeneCardsGC11M046406.
HGNCHGNC:1953. CHRM4.
HPACAB022444.
MIM118495. gene.
neXtProtNX_P08173.
PharmGKBPA26485.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG250863.
HOGENOMHOG000231484.
HOVERGENHBG105720.
KOK04132.
OMACIPDTVW.
OrthoDBEOG7V49Z7.
PhylomeDBP08173.
TreeFamTF320495.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP08173.
CleanExHS_CHRM4.
GenevestigatorP08173.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERPTHR24249:SF60. PTHR24249:SF60. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMuscarinic_acetylcholine_receptor_M4.
GenomeRNAi1132.
NextBio4708.
PROP08173.
SOURCESearch...

Entry information

Entry nameACM4_HUMAN
AccessionPrimary (citable) accession number: P08173
Secondary accession number(s): B2RPP4, Q0VD60, Q4VBK7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: September 19, 2002
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries