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Protein

Muscarinic acetylcholine receptor M4

Gene

CHRM4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

GO - Molecular functioni

  • G-protein coupled acetylcholine receptor activity Source: ProtInc

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  • cell proliferation Source: ProtInc
  • cell surface receptor signaling pathway Source: ProtInc
  • G-protein coupled acetylcholine receptor signaling pathway Source: ProtInc
  • phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  • regulation of locomotion Source: InterPro
  • signal transduction Source: ProtInc
  • synaptic transmission, cholinergic Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-390648. Muscarinic acetylcholine receptors.
R-HSA-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Gene namesi
Name:CHRM4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:1953. CHRM4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 31ExtracellularBy similarityAdd BLAST31
Transmembranei32 – 54Helical; Name=1By similarityAdd BLAST23
Topological domaini55 – 68CytoplasmicBy similarityAdd BLAST14
Transmembranei69 – 89Helical; Name=2By similarityAdd BLAST21
Topological domaini90 – 106ExtracellularBy similarityAdd BLAST17
Transmembranei107 – 128Helical; Name=3By similarityAdd BLAST22
Topological domaini129 – 148CytoplasmicBy similarityAdd BLAST20
Transmembranei149 – 171Helical; Name=4By similarityAdd BLAST23
Topological domaini172 – 193ExtracellularBy similarityAdd BLAST22
Transmembranei194 – 216Helical; Name=5By similarityAdd BLAST23
Topological domaini217 – 401CytoplasmicBy similarityAdd BLAST185
Transmembranei402 – 422Helical; Name=6By similarityAdd BLAST21
Topological domaini423 – 436ExtracellularBy similarityAdd BLAST14
Transmembranei437 – 456Helical; Name=7By similarityAdd BLAST20
Topological domaini457 – 479CytoplasmicBy similarityAdd BLAST23

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
  • postsynaptic membrane Source: UniProtKB-SubCell
  • synapse Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi1132.
OpenTargetsiENSG00000180720.
PharmGKBiPA26485.

Chemistry databases

ChEMBLiCHEMBL1821.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00835. Brompheniramine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00363. Clozapine.
DB00785. Cryptenamine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB01142. Doxepin.
DB06702. Fesoterodine.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB01625. Isopropamide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB01403. Methotrimeprazine.
DB00340. Metixene.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB00715. Paroxetine.
DB00454. Pethidine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB01036. Tolterodine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB09076. Umeclidinium.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi16.

Polymorphism and mutation databases

BioMutaiCHRM4.
DMDMi23503039.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690371 – 479Muscarinic acetylcholine receptor M4Add BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi8N-linked (GlcNAc...)Sequence analysis1
Glycosylationi13N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi105 ↔ 185PROSITE-ProRule annotation
Modified residuei459PhosphothreonineSequence analysis1
Modified residuei463PhosphothreonineSequence analysis1
Modified residuei477PhosphothreonineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP08173.
PeptideAtlasiP08173.
PRIDEiP08173.

PTM databases

iPTMnetiP08173.
PhosphoSitePlusiP08173.

Expressioni

Gene expression databases

BgeeiENSG00000180720.
CleanExiHS_CHRM4.
GenevisibleiP08173. HS.

Organism-specific databases

HPAiCAB022444.

Interactioni

Protein-protein interaction databases

BioGridi107554. 56 interactors.
DIPiDIP-61455N.
STRINGi9606.ENSP00000409378.

Chemistry databases

BindingDBiP08173.

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 59Combined sources34
Helixi61 – 63Combined sources3
Helixi66 – 83Combined sources18
Helixi85 – 95Combined sources11
Helixi101 – 135Combined sources35
Turni137 – 140Combined sources4
Helixi141 – 143Combined sources3
Helixi146 – 169Combined sources24
Helixi171 – 175Combined sources5
Helixi188 – 190Combined sources3
Helixi193 – 203Combined sources11
Helixi205 – 220Combined sources16
Beta strandi223 – 225Combined sources3
Helixi397 – 422Combined sources26
Turni423 – 425Combined sources3
Helixi432 – 456Combined sources25
Helixi458 – 468Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DSGX-ray2.60A/B22-226[»]
A/B390-478[»]
ProteinModelPortaliP08173.
SMRiP08173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08173.
KOiK04132.
OMAiMCQYRNI.
OrthoDBiEOG091G06VI.
PhylomeDBiP08173.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFTPVNGS SGNQSVRLVT SSSHNRYETV EMVFIATVTG SLSLVTVVGN
60 70 80 90 100
ILVMLSIKVN RQLQTVNNYF LFSLACADLI IGAFSMNLYT VYIIKGYWPL
110 120 130 140 150
GAVVCDLWLA LDYVVSNASV MNLLIISFDR YFCVTKPLTY PARRTTKMAG
160 170 180 190 200
LMIAAAWVLS FVLWAPAILF WQFVVGKRTV PDNQCFIQFL SNPAVTFGTA
210 220 230 240 250
IAAFYLPVVI MTVLYIHISL ASRSRVHKHR PEGPKEKKAK TLAFLKSPLM
260 270 280 290 300
KQSVKKPPPG EAAREELRNG KLEEAPPPAL PPPPRPVADK DTSNESSSGS
310 320 330 340 350
ATQNTKERPA TELSTTEATT PAMPAPPLQP RALNPASRWS KIQIVTKQTG
360 370 380 390 400
NECVTAIEIV PATPAGMRPA ANVARKFASI ARNQVRKKRQ MAARERKVTR
410 420 430 440 450
TIFAILLAFI LTWTPYNVMV LVNTFCQSCI PDTVWSIGYW LCYVNSTINP
460 470
ACYALCNATF KKTFRHLLLC QYRNIGTAR
Length:479
Mass (Da):53,049
Last modified:September 19, 2002 - v2
Checksum:i9C1B05B359A64289
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16405 Genomic DNA. Translation: AAA51571.2.
X15265 Genomic DNA. Translation: CAA33336.1.
AF498918 mRNA. Translation: AAM18941.1.
CH471064 Genomic DNA. Translation: EAW68004.1.
BC095546 mRNA. Translation: AAH95546.1.
BC119775 mRNA. Translation: AAI19776.1.
BC119817 mRNA. Translation: AAI19818.1.
BC137535 mRNA. Translation: AAI37536.1.
CCDSiCCDS44581.1.
PIRiS10127.
RefSeqiNP_000732.2. NM_000741.3.
UniGeneiHs.248100.

Genome annotation databases

EnsembliENST00000433765; ENSP00000409378; ENSG00000180720.
GeneIDi1132.
KEGGihsa:1132.
UCSCiuc001nct.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16405 Genomic DNA. Translation: AAA51571.2.
X15265 Genomic DNA. Translation: CAA33336.1.
AF498918 mRNA. Translation: AAM18941.1.
CH471064 Genomic DNA. Translation: EAW68004.1.
BC095546 mRNA. Translation: AAH95546.1.
BC119775 mRNA. Translation: AAI19776.1.
BC119817 mRNA. Translation: AAI19818.1.
BC137535 mRNA. Translation: AAI37536.1.
CCDSiCCDS44581.1.
PIRiS10127.
RefSeqiNP_000732.2. NM_000741.3.
UniGeneiHs.248100.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DSGX-ray2.60A/B22-226[»]
A/B390-478[»]
ProteinModelPortaliP08173.
SMRiP08173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107554. 56 interactors.
DIPiDIP-61455N.
STRINGi9606.ENSP00000409378.

Chemistry databases

BindingDBiP08173.
ChEMBLiCHEMBL1821.
DrugBankiDB08897. Aclidinium.
DB00321. Amitriptyline.
DB00543. Amoxapine.
DB01238. Aripiprazole.
DB00572. Atropine.
DB00835. Brompheniramine.
DB01239. Chlorprothixene.
DB00568. Cinnarizine.
DB00363. Clozapine.
DB00785. Cryptenamine.
DB00496. Darifenacin.
DB01151. Desipramine.
DB01142. Doxepin.
DB06702. Fesoterodine.
DB00725. Homatropine Methylbromide.
DB00424. Hyoscyamine.
DB00458. Imipramine.
DB01625. Isopropamide.
DB01221. Ketamine.
DB00408. Loxapine.
DB00934. Maprotiline.
DB01403. Methotrimeprazine.
DB00340. Metixene.
DB00622. Nicardipine.
DB00540. Nortriptyline.
DB00334. Olanzapine.
DB00715. Paroxetine.
DB00454. Pethidine.
DB00387. Procyclidine.
DB00420. Promazine.
DB01069. Promethazine.
DB00777. Propiomazine.
DB01224. Quetiapine.
DB00747. Scopolamine.
DB01591. Solifenacin.
DB01036. Tolterodine.
DB00376. Trihexyphenidyl.
DB00726. Trimipramine.
DB00809. Tropicamide.
DB09076. Umeclidinium.
DB00246. Ziprasidone.
GuidetoPHARMACOLOGYi16.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP08173.
PhosphoSitePlusiP08173.

Polymorphism and mutation databases

BioMutaiCHRM4.
DMDMi23503039.

Proteomic databases

PaxDbiP08173.
PeptideAtlasiP08173.
PRIDEiP08173.

Protocols and materials databases

DNASUi1132.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000433765; ENSP00000409378; ENSG00000180720.
GeneIDi1132.
KEGGihsa:1132.
UCSCiuc001nct.2. human.

Organism-specific databases

CTDi1132.
DisGeNETi1132.
GeneCardsiCHRM4.
HGNCiHGNC:1953. CHRM4.
HPAiCAB022444.
MIMi118495. gene.
neXtProtiNX_P08173.
OpenTargetsiENSG00000180720.
PharmGKBiPA26485.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP08173.
KOiK04132.
OMAiMCQYRNI.
OrthoDBiEOG091G06VI.
PhylomeDBiP08173.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-HSA-390648. Muscarinic acetylcholine receptors.
R-HSA-418594. G alpha (i) signalling events.

Miscellaneous databases

GeneWikiiMuscarinic_acetylcholine_receptor_M4.
GenomeRNAii1132.
PROiP08173.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000180720.
CleanExiHS_CHRM4.
GenevisibleiP08173. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM4_HUMAN
AccessioniPrimary (citable) accession number: P08173
Secondary accession number(s): B2RPP4, Q0VD60, Q4VBK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: September 19, 2002
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.