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Protein

Esterase-6

Gene

Est-6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transferred from the ejaculatory bulbs of males to the female genitals upon copulation, plays an important role in the reproductive biology.

Catalytic activityi

A carboxylic ester + H2O = an alcohol + a carboxylate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei209Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei466Charge relay systemBy similarity1

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: FlyBase
  • short-chain carboxylesterase activity Source: FlyBase

GO - Biological processi

  • courtship behavior Source: FlyBase
  • mating Source: FlyBase
  • multicellular organism reproduction Source: FlyBase
  • organic substance catabolic process Source: FlyBase
  • ovulation Source: FlyBase
  • pheromone biosynthetic process Source: FlyBase
  • regulation of female receptivity, post-mating Source: FlyBase
  • regulation of oviposition Source: FlyBase
  • sperm storage Source: FlyBase

Keywordsi

Molecular functionHydrolase, Serine esterase

Protein family/group databases

ESTHERidrome-este6 Carb_B_Arthropoda
MEROPSiS09.947

Names & Taxonomyi

Protein namesi
Recommended name:
Esterase-6 (EC:3.1.1.1)
Short name:
Est-6
Alternative name(s):
Carboxylic-ester hydrolase 6
Short name:
Carboxylesterase-6
Gene namesi
Name:Est-6
Synonyms:EST6
ORF Names:CG6917
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0000592 Est-6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000000856122 – 544Esterase-6Add BLAST523

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi86 ↔ 105By similarity
Disulfide bondi261 ↔ 273By similarity
Glycosylationi420N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi456N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi506N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi514 ↔ 535Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP08171
PRIDEiP08171

PTM databases

iPTMnetiP08171

Expressioni

Tissue specificityi

Specifically expressed in the ejaculatory bulbs of male.

Gene expression databases

BgeeiFBgn0000592
ExpressionAtlasiP08171 baseline and differential
GenevisibleiP08171 DM

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi64746, 1 interactor
DIPiDIP-21801N
IntActiP08171, 1 interactor
STRINGi7227.FBpp0305575

Structurei

Secondary structure

1544
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni26 – 28Combined sources3
Beta strandi29 – 32Combined sources4
Beta strandi35 – 38Combined sources4
Beta strandi43 – 52Combined sources10
Helixi59 – 61Combined sources3
Beta strandi86 – 89Combined sources4
Beta strandi99 – 103Combined sources5
Beta strandi107 – 113Combined sources7
Beta strandi121 – 127Combined sources7
Turni131 – 133Combined sources3
Helixi137 – 139Combined sources3
Helixi143 – 148Combined sources6
Beta strandi152 – 156Combined sources5
Helixi161 – 165Combined sources5
Helixi177 – 186Combined sources10
Helixi193 – 196Combined sources4
Beta strandi198 – 208Combined sources11
Helixi210 – 219Combined sources10
Helixi224 – 226Combined sources3
Beta strandi231 – 235Combined sources5
Turni241 – 243Combined sources3
Helixi248 – 258Combined sources11
Helixi267 – 270Combined sources4
Helixi279 – 284Combined sources6
Helixi285 – 287Combined sources3
Beta strandi291 – 294Combined sources4
Helixi318 – 322Combined sources5
Beta strandi332 – 337Combined sources6
Helixi342 – 345Combined sources4
Helixi346 – 349Combined sources4
Helixi357 – 360Combined sources4
Helixi361 – 372Combined sources12
Helixi384 – 389Combined sources6
Turni394 – 398Combined sources5
Turni403 – 405Combined sources3
Helixi406 – 417Combined sources12
Helixi419 – 430Combined sources12
Beta strandi437 – 443Combined sources7
Helixi450 – 455Combined sources6
Beta strandi458 – 460Combined sources3
Turni466 – 469Combined sources4
Helixi470 – 473Combined sources4
Beta strandi477 – 479Combined sources3
Helixi485 – 503Combined sources19
Beta strandi526 – 531Combined sources6
Beta strandi534 – 539Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5THMX-ray2.15A22-544[»]
ProteinModelPortaliP08171
SMRiP08171
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1516 Eukaryota
COG2272 LUCA
GeneTreeiENSGT00680000100015
InParanoidiP08171
KOiK01044
OMAiIVLSCLW
OrthoDBiEOG091G046C
PhylomeDBiP08171

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002018 CarbesteraseB
IPR019826 Carboxylesterase_B_AS
IPR019819 Carboxylesterase_B_CS
PfamiView protein in Pfam
PF00135 COesterase, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00122 CARBOXYLESTERASE_B_1, 1 hit
PS00941 CARBOXYLESTERASE_B_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P08171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYVGLGLII VLSCLWLGSN ASDTDDPLLV QLPQGKLRGR DNGSYYSYES
60 70 80 90 100
IPYAEPPTGD LRFEAPEPYK QKWSDIFDAT KTPVACLQWD QFTPGANKLV
110 120 130 140 150
GEEDCLTVSV YKPKNSKRNS FPVVAHIHGG AFMFGAAWQN GHENVMREGK
160 170 180 190 200
FILVKISYRL GPLGFVSTGD RDLPGNYGLK DQRLALKWIK QNIASFGGEP
210 220 230 240 250
QNVLLVGHSA GGASVHLQML REDFGQLARA AFSFSGNALD PWVIQKGARG
260 270 280 290 300
RAFELGRNVG CESAEDSTSL KKCLKSKPAS ELVTAVRKFL IFSYVPFAPF
310 320 330 340 350
SPVLEPSDAP DAIITQDPRD VIKSGKFGQV PWAVSYVTED GGYNAALLLK
360 370 380 390 400
ERKSGIVIDD LNERWLELAP YLLFYRDTKT KKDMDDYSRK IKQEYIGNQR
410 420 430 440 450
FDIESYSELQ RLFTDILFKN STQESLDLHR KYGKSPAYAY VYDNPAEKGI
460 470 480 490 500
AQVLANRTDY DFGTVHGDDY FLIFENFVRD VEMRPDEQII SRNFINMLAD
510 520 530 540
FASSDNGSLK YGECDFKDNV GSEKFQLLAI YIDGCQNRQH VEFP
Length:544
Mass (Da):61,125
Last modified:November 1, 1990 - v2
Checksum:i5D99B80DF588F268
GO

Sequence cautioni

The sequence AAM11419 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti519N → S in AAA28517 (PubMed:3106966).Curated1
Sequence conflicti534 – 544GCQNRQHVEFP → AARIGSMWNFRKLHE in AAA28517 (PubMed:3106966).CuratedAdd BLAST11

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti10I → T in strain: 178.7, 357F, 517S, Bar-F-77F, Bar-F-79F, Bar-F-96S, DPF-13, DPF-30, DPF-82.1, EM-10, F-274F, F-517F, F-1461S, MA-4.4 and Zim-F-H31. 1
Natural varianti24T → I in strain: 178.7, 377F, 357F, 517S, Bar-F-79F, DPF-13, DPF-30, DPF-82.1, EM-10, F-274F, F-517F, F-775F, F-1461S, MA-4.4, Ven-S-13F, Zim-F-H27, Zim-F-H31, Zim-F-S11, Zim-F-S18, Zim-F-S53, Zim-S-S2, Zim-S-S30 and Zim-S-S34. 1
Natural varianti33P → S in strain: Zim-S-H13. 1
Natural varianti58T → I in strain: 178.7, 357F, 517S, Bar-F-77F, Bar-F-79F, Bar-F-96S, DPF-13, DPF-30, DPF-82.1, EM-10, F-274F, F-1461S, MA-4.2, VC-805, Ven-S-1F, Ven-S-2F, Ven-S-3F, Ven-S-11F, Ven-S-21F, Zim-F-H31, Zim-F-S18 and Zim-S-S10. 1
Natural varianti120S → T in strain: DPF-2 and 521S. 1
Natural varianti258N → D in strain: 178.7, 357F, 517S, 709.6, Bar-F-7F, Bar-F-77F, Bar-F-79F, Bar-F-93F, Bar-F-96S, DPF-13, DPF-30, DPF-46, DPF-77, DPF-82.1, EM-10, F-96S, F-274F, F-517F, F-531F, F-611F, F-775F, F-1461S, MA-4.2, Zim-F-H27, Zim-F-H31, Zim-F-S11, Zim-F-S18 and Zim-F-S53. 1
Natural varianti268T → A in strain: 174F, 178.7, 357F, 517S, 709.6, Bar-F-7F, Bar-F-77F, Bar-F-79F, Bar-F-93F, Bar-F-96S, Bar-S-24F, Bar-S-60F, Bar-S-78F, Bar-S-89F, Bar-S-99F, Bar-S-95F, DPF-13, DPF-30, DPF-46, DPF-77, DPF-82.1, EM-10, F-96S, F-274F, F-517F, F-531F, S-549S, F-611F, F-775F, F-1461S, MA-4.2, S-114S, S-2588S, VC-815, Ven-S-1F, Ven-S-2F, Ven-S-3F, Ven-S-4F, Ven-S-11F, Ven-S-21F, Zim-F-H27, Zim-F-H31, Zim-F-S11, Zim-F-S18, Zim-F-S53, Zim-S-S34 and Zim-S-44F. 1
Natural varianti276S → P in strain: VC-805. 1
Natural varianti309A → V in strain: Ven-S-13F. 1
Natural varianti356I → T in strain: F-775F. 1
Natural varianti363E → D in strain: F-775F. 1
Natural varianti371Y → H in strain: F-775F. 1
Natural varianti376R → G in strain: Zim-S-S34. 1
Natural varianti394E → D in strain: Ven-S-13F and Zim-F-S18. 1
Natural varianti397G → R in strain: US-255F. 1
Natural varianti409L → V in strain: 357F, 517S, Bar-F-7F, Bar-F-77F, Bar-F-79F, Bar-F-93F, Bar-F-96S, DPF-13, DPF-30, F-96S, F-274F, F-517F, F-1461S, S-114S, S-549S, S-2588S, VC-805, Ven-S-4F, Zim-S-S2, Zim-S-S30 and Zim-S-S34. 1
Natural varianti441V → I in strain: Zim-F-S53. 1
Natural varianti492R → K in strain: 94F, 174F, Bar-S-24F, Bar-S-89F, Bar-S-99F, Bar-S-95F, Bar-S-60F, F-531F, F-611F, MA-4.2, Zim-F-H27, Zim-F-S18 and Zim-S-44F. 1
Natural varianti500D → N in strain: Zim-F-S18, Zim-S-H32 and Zim-S-S2. 1
Natural varianti508S → A in strain: 357F, 517S, Bar-F-79F, Bar-S-78F, DPF-13, DPF-30, F-96S, F-517F, F-1461S, MA-4.2, VC-805, Ven-S-1F, Ven-S-2F, Ven-S-3F, Ven-S-11F, Ven-S-21F and Zim-F-S11. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33780 Genomic DNA Translation: AAA28519.1
J04167 Genomic DNA Translation: AAA28518.1
M15961 mRNA Translation: AAA28517.1
AF147095 Genomic DNA Translation: AAD39958.1
AF147096 Genomic DNA Translation: AAD39959.1
AF147097 Genomic DNA Translation: AAD39960.1
AF147098 Genomic DNA Translation: AAD39961.1
AF147099 Genomic DNA Translation: AAD39962.1
AF147100 Genomic DNA Translation: AAD39963.1
AF147101 Genomic DNA Translation: AAD39964.1
AF147102 Genomic DNA Translation: AAD39965.1
AF217624 Genomic DNA Translation: AAF61043.1
AF217625 Genomic DNA Translation: AAF61044.1
AF217626 Genomic DNA Translation: AAF61045.1
AF217627 Genomic DNA Translation: AAF61046.1
AF217628 Genomic DNA Translation: AAF61047.1
AF217629 Genomic DNA Translation: AAF61048.1
AF217630 Genomic DNA Translation: AAF61049.1
AF217631 Genomic DNA Translation: AAF61050.1
AF217632 Genomic DNA Translation: AAF61051.1
AF217633 Genomic DNA Translation: AAF61052.1
AF217634 Genomic DNA Translation: AAF61053.1
AF217635 Genomic DNA Translation: AAF61054.1
AF217636 Genomic DNA Translation: AAF61055.1
AF217637 Genomic DNA Translation: AAF61056.1
AF217638 Genomic DNA Translation: AAF61057.1
AF217639 Genomic DNA Translation: AAF61058.1
AF217640 Genomic DNA Translation: AAF61059.1
AF217641 Genomic DNA Translation: AAF61060.1
AF217642 Genomic DNA Translation: AAF61061.1
AF217643 Genomic DNA Translation: AAF61062.1
AF217644 Genomic DNA Translation: AAF61063.1
AF217645 Genomic DNA Translation: AAF61064.1
AY247664 Genomic DNA Translation: AAP20953.1
AY247665 Genomic DNA Translation: AAP20954.1
AY247666 Genomic DNA Translation: AAP20955.1
AY247667 Genomic DNA Translation: AAP20956.1
AY247668 Genomic DNA Translation: AAP20957.1
AY247669 Genomic DNA Translation: AAP20958.1
AY247670 Genomic DNA Translation: AAP20959.1
AY247671 Genomic DNA Translation: AAP20960.1
AY247672 Genomic DNA Translation: AAP20961.1
AY247673 Genomic DNA Translation: AAP20962.1
AY247674 Genomic DNA Translation: AAP20963.1
AY247675 Genomic DNA Translation: AAP20964.1
AY247676 Genomic DNA Translation: AAP20965.1
AY247677 Genomic DNA Translation: AAP20966.1
AY247678 Genomic DNA Translation: AAP20967.1
AY247679 Genomic DNA Translation: AAP20968.1
AY247680 Genomic DNA Translation: AAP20969.1
AY247681 Genomic DNA Translation: AAP20970.1
AY247682 Genomic DNA Translation: AAP20971.1
AY247683 Genomic DNA Translation: AAP20972.1
AY247684 Genomic DNA Translation: AAP20973.1
AY247685 Genomic DNA Translation: AAP20974.1
AY247686 Genomic DNA Translation: AAP20975.1
AY247687 Genomic DNA Translation: AAP20976.1
AY247688 Genomic DNA Translation: AAP20977.1
AY247689 Genomic DNA Translation: AAP20978.1
AY247690 Genomic DNA Translation: AAP20979.1
AY247691 Genomic DNA Translation: AAP20980.1
AY247692 Genomic DNA Translation: AAP20981.1
AY247693 Genomic DNA Translation: AAP20982.1
AY247694 Genomic DNA Translation: AAP20983.1
AY247695 Genomic DNA Translation: AAP20984.1
AY247696 Genomic DNA Translation: AAP20985.1
AY247697 Genomic DNA Translation: AAP20986.1
AY247698 Genomic DNA Translation: AAP20987.1
AY247699 Genomic DNA Translation: AAP20988.1
AY247700 Genomic DNA Translation: AAP20989.1
AY247701 Genomic DNA Translation: AAP20990.1
AY247702 Genomic DNA Translation: AAP20991.1
AY247703 Genomic DNA Translation: AAP20992.1
AY247704 Genomic DNA Translation: AAP20993.1
AY247705 Genomic DNA Translation: AAP20994.1
AY247706 Genomic DNA Translation: AAP20995.1
AY247707 Genomic DNA Translation: AAP20996.1
AY247708 Genomic DNA Translation: AAP20997.1
AY247709 Genomic DNA Translation: AAP20998.1
AY247710 Genomic DNA Translation: AAP20999.1
AY247711 Genomic DNA Translation: AAP21000.1
AY247712 Genomic DNA Translation: AAP21001.1
AY247713 Genomic DNA Translation: AAP21002.1
AE014296 Genomic DNA Translation: AAF49946.1
AY095091 mRNA Translation: AAM11419.1 Sequence problems.
U57474 Genomic DNA Translation: AAB46692.1
U57475 Genomic DNA Translation: AAB46693.1
U57476 Genomic DNA Translation: AAB46694.1
U57477 Genomic DNA Translation: AAB46695.1
U57478 Genomic DNA Translation: AAB46696.1
U57479 Genomic DNA Translation: AAB46697.1
U57480 Genomic DNA Translation: AAB46698.1
U57481 Genomic DNA Translation: AAB46699.1
U57482 Genomic DNA Translation: AAB46700.1
U57483 Genomic DNA Translation: AAB46701.1
U57484 Genomic DNA Translation: AAB46702.1
U57485 Genomic DNA Translation: AAB46703.1
U57486 Genomic DNA Translation: AAB46704.1
U57487 Genomic DNA Translation: AAB46705.1
U57488 Genomic DNA Translation: AAB46706.1
PIRiA28022
A34089
A40122
A41426
B40122
B41426
C41426
D41426
E41426
F41426
G41426
H41426
I41426
RefSeqiNP_001261749.1, NM_001274820.1
NP_788500.1, NM_176322.3
UniGeneiDm.2828

Genome annotation databases

EnsemblMetazoaiFBtr0076003; FBpp0075735; FBgn0000592
FBtr0333383; FBpp0305575; FBgn0000592
GeneIDi39392
KEGGidme:Dmel_CG6917

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiEST6_DROME
AccessioniPrimary (citable) accession number: P08171
Secondary accession number(s): P91646
, P91647, P91648, P91649, P91650, P92173, P92195, P92200, Q867G7, Q867V2, Q867Y9, Q868B8, Q86CX4, Q86CX5, Q86CX6, Q86CX7, Q86CX8, Q86CX9, Q86CY0, Q86CY1, Q86CY2, Q86CY3, Q86CY4, Q86CY5, Q86CY6, Q86CY7, Q8SWT4, Q9U797, Q9U798, Q9U799, Q9U7A0, Q9V3U8, Q9XTN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: November 1, 1990
Last modified: May 23, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health