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Protein

S-arrestin

Gene

SAG

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated-phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. Isoform B plays a role in the phototransduction cascade.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-BTA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
S-arrestin
Alternative name(s):
48 kDa protein
Retinal S-antigen
Short name:
S-AG
Rod photoreceptor arrestin
Cleaved into the following chain:
Gene namesi
Name:SAG
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Involvement in diseasei

S-antigen induces autoimmune uveitis.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85F → A: Abrogates tetramerization, reduces dimerization, does not affect binding to microtubules and to phosphorylated light-activated rhodopsin; when associated with A-197. 1 Publication1
Mutagenesisi197F → A: Abrogates tetramerization, reduces dimerization, does not affect binding to microtubules and to phosphorylated light-activated rhodopsin; when associated with A-85. 1 Publication1

Keywords - Diseasei

Autoimmune uveitis

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000023091 – 404S-arrestinAdd BLAST404
ChainiPRO_00000023115 – 404S-arrestin short formAdd BLAST400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine; partial1 Publication1
Disulfide bondi128 ↔ 143Curated
Modified residuei230PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP08168.
PRIDEiP08168.

Expressioni

Tissue specificityi

Retina and pineal gland. Isoform B is localized in the photoreceptor outer segment.

Gene expression databases

BgeeiENSBTAG00000021480.
ExpressionAtlasiP08168. differential.

Interactioni

Subunit structurei

Monomer. Homodimer. Homotetramer. Self-association is cooperative.1 Publication

Protein-protein interaction databases

BioGridi158306. 1 interactor.
DIPiDIP-47525N.
IntActiP08168. 3 interactors.
STRINGi9913.ENSBTAP00000028633.

Structurei

Secondary structure

1404
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni8 – 11Combined sources4
Beta strandi13 – 16Combined sources4
Beta strandi20 – 27Combined sources8
Beta strandi29 – 33Combined sources5
Beta strandi41 – 47Combined sources7
Helixi49 – 52Combined sources4
Beta strandi56 – 67Combined sources12
Helixi73 – 75Combined sources3
Beta strandi79 – 92Combined sources14
Beta strandi95 – 97Combined sources3
Helixi102 – 111Combined sources10
Beta strandi115 – 120Combined sources6
Beta strandi129 – 132Combined sources4
Beta strandi143 – 155Combined sources13
Beta strandi158 – 163Combined sources6
Helixi166 – 168Combined sources3
Beta strandi169 – 179Combined sources11
Beta strandi189 – 195Combined sources7
Beta strandi202 – 210Combined sources9
Beta strandi212 – 215Combined sources4
Beta strandi220 – 228Combined sources9
Beta strandi230 – 232Combined sources3
Beta strandi234 – 247Combined sources14
Beta strandi249 – 251Combined sources3
Beta strandi254 – 263Combined sources10
Beta strandi272 – 280Combined sources9
Helixi284 – 286Combined sources3
Beta strandi291 – 293Combined sources3
Beta strandi295 – 297Combined sources3
Beta strandi301 – 303Combined sources3
Helixi318 – 321Combined sources4
Beta strandi322 – 335Combined sources14
Turni336 – 341Combined sources6
Beta strandi345 – 357Combined sources13
Beta strandi375 – 381Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AYRX-ray3.30A/B/C/D1-367[»]
1CF1X-ray2.80A/B/C/D2-404[»]
1Y6Ymodel-B10-362[»]
3UGUX-ray1.85A1-370[»]
3UGXX-ray2.65A/B/C/D1-404[»]
4J2QX-ray3.00A/B1-369[»]
4PXFX-ray2.75B67-77[»]
4ZRGX-ray2.70A1-404[»]
ProteinModelPortaliP08168.
SMRiP08168.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08168.

Family & Domainsi

Sequence similaritiesi

Belongs to the arrestin family.Curated

Phylogenomic databases

eggNOGiKOG3865. Eukaryota.
ENOG410XR0F. LUCA.
GeneTreeiENSGT00390000013152.
HOGENOMiHOG000231319.
HOVERGENiHBG002399.
InParanoidiP08168.
KOiK19627.
OMAiKEIYFHG.
OrthoDBiEOG091G05M2.
TreeFamiTF314260.

Family and domain databases

Gene3Di2.60.40.640. 1 hit.
2.60.40.840. 1 hit.
InterProiIPR000698. Arrestin.
IPR011021. Arrestin-like_N.
IPR014752. Arrestin_C.
IPR011022. Arrestin_C-like.
IPR017864. Arrestin_CS.
IPR014753. Arrestin_N.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR11792. PTHR11792. 1 hit.
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
PRINTSiPR00309. ARRESTIN.
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 2 hits.
PROSITEiPS00295. ARRESTINS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P08168-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKANKPAPNH VIFKKISRDK SVTIYLGKRD YIDHVERVEP VDGVVLVDPE
60 70 80 90 100
LVKGKRVYVS LTCAFRYGQE DIDVMGLSFR RDLYFSQVQV FPPVGASGAT
110 120 130 140 150
TRLQESLIKK LGANTYPFLL TFPDYLPCSV MLQPAPQDVG KSCGVDFEIK
160 170 180 190 200
AFATHSTDVE EDKIPKKSSV RLLIRKVQHA PRDMGPQPRA EASWQFFMSD
210 220 230 240 250
KPLRLAVSLS KEIYYHGEPI PVTVAVTNST EKTVKKIKVL VEQVTNVVLY
260 270 280 290 300
SSDYYIKTVA AEEAQEKVPP NSSLTKTLTL VPLLANNRER RGIALDGKIK
310 320 330 340 350
HEDTNLASST IIKEGIDKTV MGILVSYQIK VKLTVSGLLG ELTSSEVATE
360 370 380 390 400
VPFRLMHPQP EDPDTAKESF QDENFVFEEF ARQNLKDAGE YKEEKTDQEA

AMDE
Length:404
Mass (Da):45,275
Last modified:August 1, 1988 - v1
Checksum:i26B1D80B652AF1EF
GO
Isoform B (identifier: P08168-2) [UniParc]FASTAAdd to basket
Also known as: p44

The sequence of this isoform differs from the canonical sequence as follows:
     370-370: F → A
     371-404: Missing.

Show »
Length:370
Mass (Da):41,162
Checksum:i0FFC995F47FEFBA8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35V → L in AAA30377 (PubMed:2950857).Curated1
Sequence conflicti119L → V in AAA30377 (PubMed:2950857).Curated1
Sequence conflicti177V → I in AAA30377 (PubMed:2950857).Curated1
Sequence conflicti317D → H in AAA30377 (PubMed:2950857).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000319370F → A in isoform B. Curated1
Alternative sequenceiVSP_000320371 – 404Missing in isoform B. CuratedAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02955 mRNA. Translation: AAA30378.1.
M15115 mRNA. Translation: AAA30377.1.
U08346 mRNA. Translation: AAA20465.1.
X03454 mRNA. Translation: CAA27179.1.
PIRiB28404. A28404.
RefSeqiNP_851343.1. NM_181000.2. [P08168-2]
XP_010802144.1. XM_010803842.2. [P08168-1]
UniGeneiBt.32317.

Genome annotation databases

EnsembliENSBTAT00000028633; ENSBTAP00000028633; ENSBTAG00000021480. [P08168-1]
ENSBTAT00000033205; ENSBTAP00000033126; ENSBTAG00000021480. [P08168-2]
GeneIDi280922.
KEGGibta:280922.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02955 mRNA. Translation: AAA30378.1.
M15115 mRNA. Translation: AAA30377.1.
U08346 mRNA. Translation: AAA20465.1.
X03454 mRNA. Translation: CAA27179.1.
PIRiB28404. A28404.
RefSeqiNP_851343.1. NM_181000.2. [P08168-2]
XP_010802144.1. XM_010803842.2. [P08168-1]
UniGeneiBt.32317.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AYRX-ray3.30A/B/C/D1-367[»]
1CF1X-ray2.80A/B/C/D2-404[»]
1Y6Ymodel-B10-362[»]
3UGUX-ray1.85A1-370[»]
3UGXX-ray2.65A/B/C/D1-404[»]
4J2QX-ray3.00A/B1-369[»]
4PXFX-ray2.75B67-77[»]
4ZRGX-ray2.70A1-404[»]
ProteinModelPortaliP08168.
SMRiP08168.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi158306. 1 interactor.
DIPiDIP-47525N.
IntActiP08168. 3 interactors.
STRINGi9913.ENSBTAP00000028633.

Proteomic databases

PaxDbiP08168.
PRIDEiP08168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000028633; ENSBTAP00000028633; ENSBTAG00000021480. [P08168-1]
ENSBTAT00000033205; ENSBTAP00000033126; ENSBTAG00000021480. [P08168-2]
GeneIDi280922.
KEGGibta:280922.

Organism-specific databases

CTDi6295.

Phylogenomic databases

eggNOGiKOG3865. Eukaryota.
ENOG410XR0F. LUCA.
GeneTreeiENSGT00390000013152.
HOGENOMiHOG000231319.
HOVERGENiHBG002399.
InParanoidiP08168.
KOiK19627.
OMAiKEIYFHG.
OrthoDBiEOG091G05M2.
TreeFamiTF314260.

Enzyme and pathway databases

ReactomeiR-BTA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

EvolutionaryTraceiP08168.

Gene expression databases

BgeeiENSBTAG00000021480.
ExpressionAtlasiP08168. differential.

Family and domain databases

Gene3Di2.60.40.640. 1 hit.
2.60.40.840. 1 hit.
InterProiIPR000698. Arrestin.
IPR011021. Arrestin-like_N.
IPR014752. Arrestin_C.
IPR011022. Arrestin_C-like.
IPR017864. Arrestin_CS.
IPR014753. Arrestin_N.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR11792. PTHR11792. 1 hit.
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
PRINTSiPR00309. ARRESTIN.
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 2 hits.
PROSITEiPS00295. ARRESTINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARRS_BOVIN
AccessioniPrimary (citable) accession number: P08168
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Arrestin binds calcium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.