Reviewed, UniProtKB/Swiss-Prot P08166 (KAD2_BOVIN)

Names and origin

Protein names

Recommended name:
    Adenylate kinase 2, mitochondrial
      Short name=AK 2
    EC=2.7.4.3
Alternative name(s):
    ATP-AMP transphosphorylase 2

Gene names

Name:

AK2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis By similarity.
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer.
Subcellular location	Mitochondrion intermembrane space.
Sequence similarities	Belongs to the adenylate kinase family. AK2 subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	nucleobase, nucleoside, nucleotide and nucleic acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial inner membrane Inferred from sequence or structural similarity. Source: AgBase mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform AK2A (identifier: P08166-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform AK2B (identifier: P08166-2)

The sequence of this isoform differs from the canonical sequence as follows:
     234-241: CKDLVMFI → S

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 241

240

Adenylate kinase 2, mitochondrial

PRO_0000158916

Regions

Nucleotide binding

24 – 32

9

ATP By similarity

Nucleotide binding

47 – 76

30

AMP By similarity

Sites

Binding site

48

1

AMP By similarity

Amino acid modifications

Modified residue

95

1

N6-acetyllysine By similarity

Disulfide bond

44 ↔ 94

Ref.6

Natural variations

Alternative sequence

234 – 241

8

CKDLVMFI → S in isoform AK2B.

VSP_002789

Secondary structure

1

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241

Helix Strand Turn

Details...

Beta strand

19 – 23

5

Helix

30 – 41

12

Beta strand

44 – 47

4

Helix

48 – 58

11

Helix

61 – 71

11

Helix

78 – 89

12

Helix

92 – 94

3

Beta strand

98 – 102

5

Helix

107 – 120

14

Beta strand

126 – 131

6

Helix

134 – 142

9

Turn

148 – 150

3

Beta strand

153 – 155

3

Turn

156 – 158

3

Turn

168 – 170

3

Helix

182 – 205

24

Beta strand

209 – 213

5

Helix

218 – 232

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform AK2A [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D9232C61C431F9F4	FASTA	241	26,497
	10 20 30 40 50 60 MAPNVPAAEP VPESPKGVRA VLLGPPGAGK GTQAPKLAKN FCVCHLATGD MLRAMVASGS 70 80 90 100 110 120 ELGKKLKATM DAGKLVSDEM VLELIEKNLE TPPCKNGFLL DGFPRTVRQA EMLDDLMEKR 130 140 150 160 170 180 KEKLDSVIEF SIPDSLLIRR ITGRLIHPQS GRSYHEEFNP PKEPMKDDIT GEPLIRRSDD 190 200 210 220 230 240 NKKALKIRLE AYHTQTTPLV EYYSKRGIHS AIDASQTPDV VFASILAAFS KATCKDLVMF I
	Isoform AK2B. Checksum: 1FC8BBCA024B7DD6	FASTA	234	25,634
	10 20 30 40 50 60 MAPNVPAAEP VPESPKGVRA VLLGPPGAGK GTQAPKLAKN FCVCHLATGD MLRAMVASGS 70 80 90 100 110 120 ELGKKLKATM DAGKLVSDEM VLELIEKNLE TPPCKNGFLL DGFPRTVRQA EMLDDLMEKR 130 140 150 160 170 180 KEKLDSVIEF SIPDSLLIRR ITGRLIHPQS GRSYHEEFNP PKEPMKDDIT GEPLIRRSDD 190 200 210 220 230 NKKALKIRLE AYHTQTTPLV EYYSKRGIHS AIDASQTPDV VFASILAAFS KATS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of two types of cDNA for mitochondrial adenylate kinase and their expression in Escherichia coli." Kishi F., Tanizawa Y., Nakazawa A. J. Biol. Chem. 262:11785-11789(1987) [PubMed: 3040716] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS AK2A AND AK2B).
[2]	"Isolation and characterization of the gene encoding bovine adenylate kinase isozyme 2." Tanaka H., Yamada M., Kishi F., Nakazawa A. Gene 93:221-227(1990) [PubMed: 2227435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM AK2B).
[4]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM AK2B). Strain: Hereford. Tissue: Testis.
[5]	"Mitochondrial adenylate kinase (AK2) from bovine heart. The complete primary structure." Frank R., Trosin M., Tomasselli A.G., Noda L., Krauth-Siegel R.L., Schirmer R.H. Eur. J. Biochem. 154:205-211(1986) [PubMed: 3002789] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-239.
[6]	"The structure of bovine mitochondrial adenylate kinase: comparison with isoenzymes in other compartments." Schlauderer G.J., Schulz G.E. Protein Sci. 5:434-441(1996) [PubMed: 8868479] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS), DISULFIDE BOND.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M16224 mRNA. Translation: AAA30364.1.
M16225 mRNA. Translation: AAA30365.1.
D90069 Genomic DNA. Translation: BAA14110.1.
D90069 Genomic DNA. Translation: BAA14109.1.
BT025476 mRNA. Translation: ABF57432.1.
BC112613 mRNA. Translation: AAI12614.1.

IPI

IPI00691138.
IPI00714525.

PIR

B29792.
JS0422.

RefSeq

NP_776314.1.

UniGene

Bt.946

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AK2	X-ray	1.92	A	2-233	[»]
2AK2	X-ray	2.10	A	2-233	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSBTAG00000017605. Bos taurus. [Contig view]

GeneID

280716.

KEGG

bta:280716.

Phylogenomic databases

HOVERGEN

P08166.

OMA

P08166. KQTEPIV.

Enzyme and pathway databases

BRENDA

2.7.4.3. 251.

Family and domain databases

InterPro

IPR006259. Adenyl_kin_sub.
IPR000850. Adenylate_kin.
IPR007862. Adenylate_kinase_Znf_lid.
[Graphical view]

PANTHER

PTHR23359. Adenylate_kin. 1 hit.

Pfam

PF00406. ADK. 1 hit.
PF05191. ADK_lid. 1 hit.
[Graphical view]

PRINTS

PR00094. ADENYLTKNASE.

ProDom

PD000657. Adenylate_kin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01351. adk. 1 hit.

PROSITE

PS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KAD2_BOVIN

Accession

Primary (citable) accession number: P08166
Secondary accession number(s): P08167, Q2KIJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)