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Protein

Adenylate kinase 2, mitochondrial

Gene

AK2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.UniRule annotation

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48AMPUniRule annotation1
Binding sitei53AMPUniRule annotation1
Binding sitei109AMPUniRule annotation1
Binding sitei144ATPUniRule annotation1
Binding sitei177AMPUniRule annotation1
Binding sitei188AMPUniRule annotation1
Binding sitei216ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 32ATPUniRule annotation6
Nucleotide bindingi74 – 76AMPUniRule annotation3
Nucleotide bindingi102 – 105AMPUniRule annotation4
Nucleotide bindingi153 – 154ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
SABIO-RKP08166.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 2, mitochondrialUniRule annotation (EC:2.7.4.3UniRule annotation)
Short name:
AK 2UniRule annotation
Alternative name(s):
ATP-AMP transphosphorylase 2UniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
Cleaved into the following chain:
Adenylate kinase 2, mitochondrial, N-terminally processedUniRule annotation
Gene namesi
Name:AK2UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004232111 – 241Adenylate kinase 2, mitochondrialAdd BLAST241
Initiator methionineiRemoved; alternateUniRule annotation1 Publication
ChainiPRO_00001589162 – 241Adenylate kinase 2, mitochondrial, N-terminally processedAdd BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Disulfide bondi44 ↔ 94UniRule annotation1 Publication
Modified residuei60PhosphoserineBy similarity1
Modified residuei64N6-succinyllysineBy similarity1
Modified residuei95N6-succinyllysineBy similarity1
Modified residuei135PhosphoserineBy similarity1
Modified residuei183N6-acetyllysineBy similarity1
Modified residuei197PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP08166.
PeptideAtlasiP08166.
PRIDEiP08166.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017605.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP08166. 1 interactor.
STRINGi9913.ENSBTAP00000023406.

Structurei

Secondary structure

1241
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 23Combined sources5
Helixi30 – 41Combined sources12
Beta strandi44 – 47Combined sources4
Helixi48 – 58Combined sources11
Helixi61 – 71Combined sources11
Helixi78 – 89Combined sources12
Helixi92 – 94Combined sources3
Beta strandi98 – 102Combined sources5
Helixi107 – 120Combined sources14
Beta strandi126 – 131Combined sources6
Helixi134 – 142Combined sources9
Turni148 – 150Combined sources3
Beta strandi153 – 155Combined sources3
Turni156 – 158Combined sources3
Turni168 – 170Combined sources3
Helixi182 – 205Combined sources24
Beta strandi209 – 213Combined sources5
Helixi218 – 232Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AK2X-ray1.92A2-233[»]
2AK2X-ray2.10A2-233[»]
ProteinModelPortaliP08166.
SMRiP08166.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP08166.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 76NMPbindAdd BLAST30
Regioni143 – 180LIDAdd BLAST38

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.

Sequence similaritiesi

Belongs to the adenylate kinase family. AK2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOGENOMiHOG000238772.
HOVERGENiHBG000458.
InParanoidiP08166.
KOiK00939.
OMAiCPKGIRA.
OrthoDBiEOG091G06BH.
TreeFamiTF300896.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03168. Adenylate_kinase_AK2. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR007862. Adenylate_kinase_lid-dom.
IPR028587. AK2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform AK2A (identifier: P08166-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPNVPAAEP VPESPKGVRA VLLGPPGAGK GTQAPKLAKN FCVCHLATGD
60 70 80 90 100
MLRAMVASGS ELGKKLKATM DAGKLVSDEM VLELIEKNLE TPPCKNGFLL
110 120 130 140 150
DGFPRTVRQA EMLDDLMEKR KEKLDSVIEF SIPDSLLIRR ITGRLIHPQS
160 170 180 190 200
GRSYHEEFNP PKEPMKDDIT GEPLIRRSDD NKKALKIRLE AYHTQTTPLV
210 220 230 240
EYYSKRGIHS AIDASQTPDV VFASILAAFS KATCKDLVMF I
Length:241
Mass (Da):26,497
Last modified:January 23, 2007 - v2
Checksum:iD9232C61C431F9F4
GO
Isoform AK2B (identifier: P08166-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     234-241: CKDLVMFI → S

Show »
Length:234
Mass (Da):25,634
Checksum:i1FC8BBCA024B7DD6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002789234 – 241CKDLVMFI → S in isoform AK2B. 3 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16224 mRNA. Translation: AAA30364.1.
M16225 mRNA. Translation: AAA30365.1.
D90069 Genomic DNA. Translation: BAA14110.1.
D90069 Genomic DNA. Translation: BAA14109.1.
BT025476 mRNA. Translation: ABF57432.1.
BC112613 mRNA. Translation: AAI12614.1.
PIRiB29792.
JS0422.
RefSeqiNP_776314.1. NM_173889.1. [P08166-2]
XP_005202939.1. XM_005202882.1. [P08166-1]
UniGeneiBt.946.

Genome annotation databases

EnsembliENSBTAT00000023406; ENSBTAP00000023406; ENSBTAG00000017605. [P08166-2]
GeneIDi280716.
KEGGibta:280716.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16224 mRNA. Translation: AAA30364.1.
M16225 mRNA. Translation: AAA30365.1.
D90069 Genomic DNA. Translation: BAA14110.1.
D90069 Genomic DNA. Translation: BAA14109.1.
BT025476 mRNA. Translation: ABF57432.1.
BC112613 mRNA. Translation: AAI12614.1.
PIRiB29792.
JS0422.
RefSeqiNP_776314.1. NM_173889.1. [P08166-2]
XP_005202939.1. XM_005202882.1. [P08166-1]
UniGeneiBt.946.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AK2X-ray1.92A2-233[»]
2AK2X-ray2.10A2-233[»]
ProteinModelPortaliP08166.
SMRiP08166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP08166. 1 interactor.
STRINGi9913.ENSBTAP00000023406.

Proteomic databases

PaxDbiP08166.
PeptideAtlasiP08166.
PRIDEiP08166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000023406; ENSBTAP00000023406; ENSBTAG00000017605. [P08166-2]
GeneIDi280716.
KEGGibta:280716.

Organism-specific databases

CTDi204.

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00830000128340.
HOGENOMiHOG000238772.
HOVERGENiHBG000458.
InParanoidiP08166.
KOiK00939.
OMAiCPKGIRA.
OrthoDBiEOG091G06BH.
TreeFamiTF300896.

Enzyme and pathway databases

ReactomeiR-BTA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
SABIO-RKP08166.

Miscellaneous databases

EvolutionaryTraceiP08166.

Gene expression databases

BgeeiENSBTAG00000017605.

Family and domain databases

CDDicd01428. ADK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk. 1 hit.
MF_03168. Adenylate_kinase_AK2. 1 hit.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR033690. Adenylat_kinase_CS.
IPR007862. Adenylate_kinase_lid-dom.
IPR028587. AK2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAD2_BOVIN
AccessioniPrimary (citable) accession number: P08166
Secondary accession number(s): P08167, Q2KIJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.