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Reviewed, UniProtKB/Swiss-Prot P08166 (KAD2_BOVIN)

Last modified February 9, 2010. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Adenylate kinase 2, mitochondrial
      Short name=AK 2
    EC=2.7.4.3
Alternative name(s):
    ATP-AMP transphosphorylase 2
Gene names
Name: AK2
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis By similarity.

Catalytic activity

ATP + AMP = 2 ADP.

Subunit structure

Monomer.

Subcellular location

Mitochondrion intermembrane space.

Sequence similarities

Belongs to the adenylate kinase family. AK2 subfamily.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform AK2A (identifier: P08166-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform AK2B (identifier: P08166-2)

The sequence of this isoform differs from the canonical sequence as follows:
     234-241: CKDLVMFI → S

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 241240Adenylate kinase 2, mitochondrial
PRO_0000158916

Regions

Nucleotide binding24 – 329ATP By similarity
Nucleotide binding47 – 7630AMP By similarity

Sites

Binding site481AMP By similarity

Amino acid modifications

Modified residue161N6-acetyllysine By similarity
Modified residue951N6-acetyllysine By similarity
Disulfide bond44 ↔ 94 Ref.6

Natural variations

Alternative sequence234 – 2418CKDLVMFI → S in isoform AK2B.
VSP_002789

Secondary structure

1 ................................... 241
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform AK2A [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: D9232C61C431F9F4

FASTA24126,497
        10         20         30         40         50         60 
MAPNVPAAEP VPESPKGVRA VLLGPPGAGK GTQAPKLAKN FCVCHLATGD MLRAMVASGS 

        70         80         90        100        110        120 
ELGKKLKATM DAGKLVSDEM VLELIEKNLE TPPCKNGFLL DGFPRTVRQA EMLDDLMEKR 

       130        140        150        160        170        180 
KEKLDSVIEF SIPDSLLIRR ITGRLIHPQS GRSYHEEFNP PKEPMKDDIT GEPLIRRSDD 

       190        200        210        220        230        240 
NKKALKIRLE AYHTQTTPLV EYYSKRGIHS AIDASQTPDV VFASILAAFS KATCKDLVMF 


I

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Isoform AK2B.

Checksum: 1FC8BBCA024B7DD6
Show »

FASTA23425,634

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References

« Hide 'large scale' references
[1]"Isolation and characterization of two types of cDNA for mitochondrial adenylate kinase and their expression in Escherichia coli."
Kishi F., Tanizawa Y., Nakazawa A.
J. Biol. Chem. 262:11785-11789(1987) [PubMed: 3040716] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS AK2A AND AK2B).
[2]"Isolation and characterization of the gene encoding bovine adenylate kinase isozyme 2."
Tanaka H., Yamada M., Kishi F., Nakazawa A.
Gene 93:221-227(1990) [PubMed: 2227435] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM AK2B).
[4]NIH - Mammalian Gene Collection (MGC) project
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM AK2B).
Strain: Hereford.
Tissue: Testis.
[5]"Mitochondrial adenylate kinase (AK2) from bovine heart. The complete primary structure."
Frank R., Trosin M., Tomasselli A.G., Noda L., Krauth-Siegel R.L., Schirmer R.H.
Eur. J. Biochem. 154:205-211(1986) [PubMed: 3002789] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-239.
[6]"The structure of bovine mitochondrial adenylate kinase: comparison with isoenzymes in other compartments."
Schlauderer G.J., Schulz G.E.
Protein Sci. 5:434-441(1996) [PubMed: 8868479] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS), DISULFIDE BOND.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M16224 mRNA. Translation: AAA30364.1.
M16225 mRNA. Translation: AAA30365.1.
D90069 Genomic DNA. Translation: BAA14110.1.
D90069 Genomic DNA. Translation: BAA14109.1.
BT025476 mRNA. Translation: ABF57432.1.
BC112613 mRNA. Translation: AAI12614.1.
IPIIPI00691138.
IPI00714525.
PIRB29792.
JS0422.
RefSeqNP_776314.1.
UniGeneBt.946

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AK2X-ray1.92A2-233[»]
2AK2X-ray2.10A2-233[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGP08166.

Genome annotation databases

EnsemblENSBTAT00000023406; ENSBTAP00000023406; ENSBTAG00000017605; Bos taurus. [Genome view]
GeneID280716.
KEGGbta:280716.

Organism-specific databases

CTD280716.

Phylogenomic databases

eggNOGmaNOG07052.
HOVERGENP08166.
InParanoidP08166.
OMAERIDQPD.
PhylomeDBP08166.

Enzyme and pathway databases

BRENDA2.7.4.3. 251.

Family and domain databases

InterProIPR006259. Adenyl_kin_sub.
IPR000850. Adenylate_kin.
IPR007862. Adenylate_kinase_lid-dom.
[Graphical view]
PANTHERPTHR23359. Adenylate_kin. 1 hit.
PfamPF00406. ADK. 1 hit.
PF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSPR00094. ADENYLTKNASE.
TIGRFAMsTIGR01351. adk. 1 hit.
PROSITEPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameKAD2_BOVIN
AccessionPrimary (citable) accession number: P08166
Secondary accession number(s): P08167, Q2KIJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: February 9, 2010
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents