Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Early growth response protein 1

Gene

Egr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri311 – 33525C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri341 – 36323C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri369 – 39123C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • double-stranded DNA binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • promoter-specific chromatin binding Source: RGD
  • RNA polymerase II core promoter sequence-specific DNA binding Source: RGD
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: RGD
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: RGD
  • transcription factor binding Source: RGD

GO - Biological processi

  • cellular response to antibiotic Source: RGD
  • cellular response to cAMP Source: RGD
  • cellular response to drug Source: RGD
  • cellular response to follicle-stimulating hormone stimulus Source: RGD
  • cellular response to gonadotropin stimulus Source: RGD
  • cellular response to growth factor stimulus Source: RGD
  • cellular response to heparin Source: UniProtKB
  • cellular response to hyperoxia Source: RGD
  • cellular response to hypoxia Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • cellular response to isoquinoline alkaloid Source: RGD
  • cellular response to mechanical stimulus Source: RGD
  • cellular response to mycophenolic acid Source: UniProtKB
  • cellular response to steroid hormone stimulus Source: RGD
  • circadian rhythm Source: RGD
  • glomerular mesangial cell proliferation Source: UniProtKB
  • learning or memory Source: RGD
  • long-term memory Source: RGD
  • long-term synaptic potentiation Source: RGD
  • oligodendrocyte differentiation Source: RGD
  • positive regulation of cell death Source: RGD
  • positive regulation of gene expression Source: RGD
  • positive regulation of glomerular metanephric mesangial cell proliferation Source: UniProtKB
  • positive regulation of neuron apoptotic process Source: RGD
  • positive regulation of smooth muscle cell migration Source: RGD
  • positive regulation of smooth muscle cell proliferation Source: RGD
  • positive regulation of transcription, DNA-templated Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of long-term neuronal synaptic plasticity Source: RGD
  • response to amphetamine Source: RGD
  • response to carbon monoxide Source: RGD
  • response to cocaine Source: RGD
  • response to drug Source: RGD
  • response to electrical stimulus Source: RGD
  • response to ethanol Source: RGD
  • response to follicle-stimulating hormone Source: RGD
  • response to gonadotropin Source: RGD
  • response to norepinephrine Source: RGD
  • response to nutrient levels Source: RGD
  • transcription from RNA polymerase II promoter Source: GOC
  • wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Early growth response protein 1
Short name:
EGR-1
Alternative name(s):
Nerve growth factor-induced protein A
Short name:
NGFI-A
Transcription factor Zif268
Zinc finger protein Krox-24
Gene namesi
Name:Egr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2544. Egr1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi265 – 2651I → F: Increases transcriptional activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508Early growth response protein 1PRO_0000047111Add
BLAST

Proteomic databases

PaxDbiP08154.

Expressioni

Inductioni

By growth factors.

Gene expression databases

GenevisibleiP08154. RN.

Interactioni

Subunit structurei

Interacts with SNAI1 and SP1 upon 12-O-tetradecanoylphorbol-13-acetate (TPA) induction.By similarity

GO - Molecular functioni

  • transcription factor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026303.

Structurei

3D structure databases

ProteinModelPortaliP08154.
SMRiP08154. Positions 308-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati413 – 42081
Repeati421 – 42882
Repeati429 – 43683
Repeati437 – 44484
Repeati445 – 45285
Repeati453 – 46086
Repeati462 – 46877

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni413 – 468567 X 8 AA tandem repeats of [TS](2)-[FY]-[PS]-S-P-[GSAV]-XAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 5628Gly/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri311 – 33525C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri341 – 36323C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri369 – 39123C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP08154.
KOiK09203.
OMAiFATQTGS.
OrthoDBiEOG7Z69C7.
PhylomeDBiP08154.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 1 hit.
PF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P08154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNYPKLEEM MLLSNGAPQF LGAAGTPEGS GGNNSSSSSS SSSGGGGGGG
60 70 80 90 100
SNSGSSAFNP QGEPSEQPYE HLTTESFSDI ALNNEKALVE TSYPSQTTRL
110 120 130 140 150
PPITYTGRFS LEPAPNSGNT LWPEPLFSLV SGLVSMTNPP TSSSSAPSPA
160 170 180 190 200
ASSSSSASQS PPLSCAVPSN DSSPIYSAAP TFPTPNTDIF PEPQSQAFPG
210 220 230 240 250
SAGTALQYPP PAYPATKGGF QVPMIPDYLF PQQQGDLSLG TPDQKPFQGL
260 270 280 290 300
ENRTQQPSLT PLSTIKAFAT QSGSQDLKAL NNTYQSQLIK PSRMRKYPNR
310 320 330 340 350
PSKTPPHERP YACPVESCDR RFSRSDELTR HIRIHTGQKP FQCRICMRNF
360 370 380 390 400
SRSDHLTTHI RTHTGEKPFA CDICGRKFAR SDERKRHTKI HLRQKDKKAD
410 420 430 440 450
KSVVASSAAS SLSSYPSPVA TSYPSPATTS FPSPVPTSYS SPGSSTYPSP
460 470 480 490 500
AHSGFPSPSV ATTYASVPPA FPAQVSTFQS AGVSNSFSTS TGLSDMTATF

SPRTIEIC
Length:508
Mass (Da):53,935
Last modified:January 1, 1990 - v2
Checksum:i3947BBA871BB8FA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18416 mRNA. Translation: AAA61927.1.
J04154 Genomic DNA. Translation: AAA60740.1.
AY551092 mRNA. Translation: AAS58455.1.
PIRiA32225.
RefSeqiNP_036683.1. NM_012551.2.
UniGeneiRn.9096.

Genome annotation databases

GeneIDi24330.
KEGGirno:24330.
UCSCiRGD:2544. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18416 mRNA. Translation: AAA61927.1.
J04154 Genomic DNA. Translation: AAA60740.1.
AY551092 mRNA. Translation: AAS58455.1.
PIRiA32225.
RefSeqiNP_036683.1. NM_012551.2.
UniGeneiRn.9096.

3D structure databases

ProteinModelPortaliP08154.
SMRiP08154. Positions 308-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026303.

Proteomic databases

PaxDbiP08154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24330.
KEGGirno:24330.
UCSCiRGD:2544. rat.

Organism-specific databases

CTDi1958.
RGDi2544. Egr1.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
HOGENOMiHOG000036856.
HOVERGENiHBG003909.
InParanoidiP08154.
KOiK09203.
OMAiFATQTGS.
OrthoDBiEOG7Z69C7.
PhylomeDBiP08154.

Miscellaneous databases

PROiP08154.

Gene expression databases

GenevisibleiP08154. RN.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR021839. DUF3432.
IPR021849. DUF3446.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF11914. DUF3432. 1 hit.
PF11928. DUF3446. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor."
    Milbrandt J.
    Science 238:797-799(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure of the NGFI-A gene and detection of upstream sequences responsible for its transcriptional induction by nerve growth factor."
    Changelian P.S., Feng P., King T.C., Milbrandt J.
    Proc. Natl. Acad. Sci. U.S.A. 86:377-381(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning and sequencing of cDNA encoding EGR1 from rat."
    Ju S.K.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Midbrain.
  4. "Transcriptional activity of the zinc finger protein NGFI-A is influenced by its interaction with a cellular factor."
    Russo M.W., Matheny C., Milbrandt J.
    Mol. Cell. Biol. 13:6858-6865(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ILE-265.

Entry informationi

Entry nameiEGR1_RAT
AccessioniPrimary (citable) accession number: P08154
Secondary accession number(s): Q53YM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 1, 1990
Last modified: June 8, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.